Objective:To document the effectiveness of ultrasound-guided genicular nerve block (GNB) in treating knee osteoarthritis (KOA).Methods:A total of 92 KOA patients were randomly divided into an observation group and a control group with 46 in each. Those in both groups were treated conventionally, including with non-steroidal anti-inflammatory drugs, acupuncture, ultrasound, laser irradiation and manipulation therapy. The observation group additionally underwent ultrasound-guided genicular nerve block treatment, once a week for 2 weeks. Pain scoring on a visual analog scale (VAS), the Western Ontario and McMaster University Osteoarthritis Index (WOMAC) and a 6-minute walk test (6MWT) were used to evaluate everyone before and after the treatment, and then 8 weeks later.Results:In the observation group the average VAS rating [(3.54±2.00) at week 2 and (4.13±2.04) at week 8] and the average WOMAC subscale and total scores [(36.91±16.91) at week 8] had improved significantly right after the experiment and 8 weeks later. But in the control group this was true only right after the treatment. The observation group also demonstrated superior improvements in 6MWT distance at week 2 [(434.22±125.19)m] and week 8 [(446.35±126.45)m] compared to both its own baseline and the control group.Conclusions:Ultrasound-guided genicular nerve block is a rapid, precise, effective, and long-lasting intervention for alleviating pain, improving knee function and enhancing walking endurance in KOA patients.

Objective:To document the effectiveness of ultrasound-guided genicular nerve block (GNB) in treating knee osteoarthritis (KOA).Methods:A total of 92 KOA patients were randomly divided into an observation group and a control group with 46 in each. Those in both groups were treated conventionally, including with non-steroidal anti-inflammatory drugs, acupuncture, ultrasound, laser irradiation and manipulation therapy. The observation group additionally underwent ultrasound-guided genicular nerve block treatment, once a week for 2 weeks. Pain scoring on a visual analog scale (VAS), the Western Ontario and McMaster University Osteoarthritis Index (WOMAC) and a 6-minute walk test (6MWT) were used to evaluate everyone before and after the treatment, and then 8 weeks later.Results:In the observation group the average VAS rating [(3.54±2.00) at week 2 and (4.13±2.04) at week 8] and the average WOMAC subscale and total scores [(36.91±16.91) at week 8] had improved significantly right after the experiment and 8 weeks later. But in the control group this was true only right after the treatment. The observation group also demonstrated superior improvements in 6MWT distance at week 2 [(434.22±125.19)m] and week 8 [(446.35±126.45)m] compared to both its own baseline and the control group.Conclusions:Ultrasound-guided genicular nerve block is a rapid, precise, effective, and long-lasting intervention for alleviating pain, improving knee function and enhancing walking endurance in KOA patients.

The liver has a complex cellular composition and a remarkable regenerative capacity. The primary cell types in the liver are two parenchymal cell populations, hepatocytes and cholangiocytes, that perform most of the functions of the liver and that are helped through interactions with non-parenchymal cell types comprising stellate cells, endothelia and various hemopoietic cell populations. The regulation of the cells in the liver is mediated by an insoluble complex of proteins and carbohydrates, the extracellular matrix, working synergistically with soluble paracrine and systemic signals. In recent years, with the rapid development of genetic sequencing technologies, research on the liver's cellular composition and its regulatory mechanisms during various conditions has been extensively explored. Meanwhile breakthroughs in strategies for cell transplantation are enabling a future in which there can be a rescue of patients with end-stage liver diseases, offering potential solutions to the chronic shortage of livers and alternatives to liver transplantation. This review will focus on the cellular mechanisms of liver homeostasis and how to select ideal sources of cells to be transplanted to achieve liver regeneration and repair. Recent advances are summarized for promoting the treatment of end-stage liver diseases by forms of cell transplantation that now include grafting strategies.
Humans ; Liver/surgery* ; Hepatocytes/transplantation* ; Stem Cells/metabolism* ; Liver Diseases/surgery*

Objective:To investigate the molecular mechanism of miR-132 regulation of neuronal apoptosis in Alzheimer′s disease model mice (AD mice).Methods:The study started and ended from 2016.01 to 2021.03. The animals were divided into the AD transgenic model mice group (AD model mice group) and the littermate negative control mice group (litter negative mice group). The number of animals in each group in the subsequent experiment was three mouses. The levels of miR-132 in the cortex and hippocampus of the two groups were detected by quantitative PCR. The TUNNEL(terminal-deoxynucleotidyl transferase-mediated nick end labeling) method was used to detect neuronal apoptosis in the brain tissue of the two groups of mice. The expression levels of apoptosis-related proteins in brain tissue of two groups of mice were detected by immunoblotting. The dual-luciferase reporter system recognizes downstream target proteins that miRNA-132 directly regulated. The independent sample Student′s t-test was used to compare the two groups, and the difference was statistically significant with P<0.05. Results:Compared with the littermate-negative mice, the relative expression of miRNA-132 in the cortex and hippocampus of AD mice was significantly down-regulated ( P<0.01). The results of TUNNEL showed a significant increase in neuronal apoptosis in the brain of AD mice (relative number of apoptotic cells ( P<0.01). Western blot results showed that the relative expression of apoptosis-related proteins in the brain of AD mice was significantly increased ( P<0.05 or P<0.01). The dual-luciferase reporter system showed that miRNA-132 could directly interact with the downstream protein FOXO3a, and the luciferase activity of the AD model group was significantly reduced ( P<0.01). Conclusions:miRNA-132 reduction may disinhibit apoptosis-related proteins BAX and Caspase-3 by up-regulating FOXO3a expression to speed up neuronal apoptosis in AD mice.

Objective:To investigate the molecular mechanism of miR-132 regulation of neuronal apoptosis in Alzheimer′s disease model mice (AD mice).Methods:The study started and ended from 2016.01 to 2021.03. The animals were divided into the AD transgenic model mice group (AD model mice group) and the littermate negative control mice group (litter negative mice group). The number of animals in each group in the subsequent experiment was three mouses. The levels of miR-132 in the cortex and hippocampus of the two groups were detected by quantitative PCR. The TUNNEL(terminal-deoxynucleotidyl transferase-mediated nick end labeling) method was used to detect neuronal apoptosis in the brain tissue of the two groups of mice. The expression levels of apoptosis-related proteins in brain tissue of two groups of mice were detected by immunoblotting. The dual-luciferase reporter system recognizes downstream target proteins that miRNA-132 directly regulated. The independent sample Student′s t-test was used to compare the two groups, and the difference was statistically significant with P<0.05. Results:Compared with the littermate-negative mice, the relative expression of miRNA-132 in the cortex and hippocampus of AD mice was significantly down-regulated ( P<0.01). The results of TUNNEL showed a significant increase in neuronal apoptosis in the brain of AD mice (relative number of apoptotic cells ( P<0.01). Western blot results showed that the relative expression of apoptosis-related proteins in the brain of AD mice was significantly increased ( P<0.05 or P<0.01). The dual-luciferase reporter system showed that miRNA-132 could directly interact with the downstream protein FOXO3a, and the luciferase activity of the AD model group was significantly reduced ( P<0.01). Conclusions:miRNA-132 reduction may disinhibit apoptosis-related proteins BAX and Caspase-3 by up-regulating FOXO3a expression to speed up neuronal apoptosis in AD mice.

Objective To investigate the expression of IL-24 in patients with non-small cell lung cancer (NSCLC),and to evaluate its influence on the bioactivity of NSCLC cells. Methods Thirty-nine patient with NSCLC (23 patients with adenocarcinoma and 16 patients with squamous carcinoma) and 17 healthy subjects were enrolled in this study. Serum samples and lung cancer tissues were collected. IL-24 expression in the serum samples was measured using enzyme-linked immunosorbent assay. Its expression at mRNA level in the lung cancer tissues was measured using reverse transcriptional real-time PCR. Adenocar-cinoma cell line A549 and squamous carcinoma cell line NCI-H520 were stimulated with recombinant human IL-24 (10 ng/ml and 100 ng/ml) for 24 hours. Cell proliferation was measured using CCK-8 method. Ap-optosis and cell cycle were measured using flow cytometry. Cell invasion was measured using Transwell as-say. Results Serum IL-24 was significantly elevated in patients with NSCLC in comparison with that in healthy subjects [(144.10±64.43) vs(48.47±18.00) pg/ml]. No significant difference in IL-24 expres-sion was found between patients with adenocarcinoma and squamous carcinoma. IL-24 expression at mRNA level in lung cancer tissues of patients with NSCLC was also significantly increased with an approximately 5-fold enhancement in comparison with that in normal lung tissues. Stimulation with low concentration of re-combinant IL-24(10 ng/ml) promoted the proliferation and suppressed the apoptosis of A549 and NCI-H520 cells. In contrast, high concentration of recombinant IL-24 (100 ng/ml) stimulation notably inhibited the proliferation and enhanced the apoptosis of lung cancer cell lines. No remarkable changes in cell cycle of the two kinds of lung cancer cells in response to IL-24 stimulation were observed. Moreover,low concentration of recombinant IL-24 (10 ng/ml) did not affect the invasion of A549 and NCI-H520 cells,while high concen-tration of recombinant IL-24 (100 ng/ml) significantly inhibited the invasion of lung cancer cells. Conclu-sion IL-24 might influence the bioactivity of NSCLC cells in a concentration-dependent manner. High con-centration of IL-24 might counteract the invasion and metastasis of NSCLC,which is important to prevent dis-ease promotion.

Objective To study the clinical effect of Cold-Knife Conization (CKC) in patients with early cervical cancer and its influence in the pregnancy outcome.Methods 40 early cervical cancer patients of adopting the CKC from January 2010 to September 2012 in our Department were selected as observation group.40 early cervical cancer patients without history of cervical conization were selected as control group.All patients were followed up for 8 to 36 months.Tumor recurrence,pregnancy and maternity history and pregnancy outcomes were recorded and compared between groups.Results In the observation group,32 cases were pregnant (the success rate of pregnancy was 80.0％),of which 4 cases were aborted and the delivery rate was 87.5％.In the control group,39 cases were pregnant(the success rate of pregnancy was 97.5％),no abortion was found,and the delivery rate was 100.0％.The pregnancy rate and delivery rate in the observation group were lower than those in the control group (P ＜ 0.05).There was no significant difference in delivery mode between the two groups (P ＞ 0.05).The observation group had 8 cases (28.6％) premature delivery,9 cases (32.1％) neonatal asphyxia,6 cases (21.4％) premature rupture of membranes,and the average weight of neonates was (2 842.17 ±48.99)g.The control group had 3 cases (7.7％) premature delivery,1 case (2.6％) neonatal asphyxia,2 cases (5.1％) premature rupture of membranes,and the average weight of neonates was (3 243.81 ±51.02)g.The perinatal premature birth rate,neonatal asphyxia rate and premature rupture rate of membranes in the observation group were higher than those in the control group,and the average weight of newborns was lower than that in the control group,with statistically significant difference (P ＜ 0.05).During the follow-up period,the recurrence rate of the observation group was lower than that ofthe control group [2.5％ (1/40) vs 17.5％ (7/40)],with statistical significant significance (P ＜ 0.05).Conclusions Although cold knife conization can reduce the recurrence rate of early cervical cancer patients,the fertility and pregnancy outcomes of patients after operation is greatly affected.Careful selection should be made in clinical treatment according to the actual situation.

Objective To establish the primary cat intestinal epithelial cells(IECs)culture methods and construct the cD-NA library for the following yeast two-hybrid experiment,so as to screen the virulence interaction factors among the final host. Methods The primary cat IECs were cultured by the tissue cultivation and combined digestion with collagenase XI and dispase I separately. Then the cat IECs cultured was identified with the morphological observation and cyto-keratin detection ,by using goat anti-cyto-keratin monoclonal antibodies. The mRNA of cat IECs was isolated and used as the template to synthesize the first strand cDNA by SMARTTM technology,and then the double-strand cDNAs were acquired by LD-PCR,which were subsequently cloned into the plasmid PGADT7-Rec to construct yeast two-hybrid cDNA library in the yeast strain Y187 by homologous recom-bination. Matchmaker?Insert Check PCR was used to detect the size distribution of cDNA fragments after the capacity calcula-tion of the cDNA library. Results The comparison of the two cultivation methods indicated that the combined digestion of colla-genase XI and dispase I was more effective than the tissue cultivation. The cat IECs system of continuous culture was established and the cat IECs with high purity were harvested for constructing the yeast two-hybrid cDNA library. The library contained 1.1× 106 independent clones. The titer was 2.8 × 109 cfu/ml. The size of inserted fragments was among 0.5-2.0 kb. Conclusion The yeast two-hybrid cDNA library of cat IECs meets the requirements of further screen research,and this study lays the foundation of screening the Toxoplasma gondii virulence interaction factors among the cDNA libraries of its final hosts.

Objective To explore the biological function of rhoptry protein 38(ROP38)of Toxoplasma gondii,and to iden?tify the reactogenicity of the recombinant protein(rROP38). Methods The ROP38 was amplified by RT?PCR from T. gondii RH strain,and was cloned into prokaryotic expression vector pET?28a(+). The recombinant plasmid was transformed into E. co?li BL21(DE3)competent cells. Then the rROP38 was analyzed by SDS?PAGE and identified by Western blot. Results SDS?PAGE showed that rROP38 was efficient expression with a molecular weight of about 43 kD. Western blot showed that rROP38 reacted with antibody of His tag or human positive antibody,which indicated that ROP38 had good reactogenicity and could be a serological diagnostic antigen. Conclusion The study successfully obtains the rROP38 of T. gondii with good reactogenicity.

Objective To detect the expression of SSX and to correlate it with clinical indicators of primary hepatocellular carcinoma (HCC).Methods The expression of SSX1-5 mRNA and SSX1 protein were respectively detected by RT-PCR and Western blot and immunohistochemistry staining.The relation between the expression of SSX mRNA and SSX1 protein with clinical indicators were analysed.Results SSX1,SSX2,and SSX3 mRNA were expressed in hepatocellular carcinoma cell lines BEL-7404,Hep G2,and SMMC-7721.In 26 HCC samples,SSX1-SSX5 mRNA was detectable in 53.8％,42.3％,50.0％,46.2％ and 26.9％.The expression of SSX1 mRNA was not related to serum AFP levels (P ＞0.05).Specific expression was both found in the normal group and the high value group.The expression rate of SSX1 mRNA was 85.7％ in the older group,which was higher than in the younger group (16.7％,P ＜ 0.05).The expression rate of SSX1 protein was 50％ in HCC tissues,which was not seen in the caner-adjacent or cirrhosis tissues.In 49 HCC paraffin tissue section samples,the expression rate of SSX1 protein was higher than that in caner-adjacent tissues (46.9％ vs 18.4％,P ＜ 0.05).The expression rate of SSX1 protein was 68.3％ in the large hepatocellular carcinoma group,which was higher than in the small hepatocellular carcinoma group (29.6％),(P ＜ 0.05).Conclusions SSX1 mRNA is expressed with a high percentage and specificity in HCC and their products are new potential promising targets for antigen-specific immunotherapy of HCC.The detection of SSX1 expression has the potential value for auxiliary diagnosis of HCC.