Objective:Taking 88 counties in Guizhou from 2021 to 2023 as the research sample,the indicator system of the dimension of Traditional Chinese Medicine(TCM)are constructed to analyze the spatial evolution and spatial effects of the allocation of TCM human resources in Guizhou.Methods:Using spatial correlation model and Vector Autoregression(VAR)model are applied to conduct the spatial autocorrelation test and impulse response study on the scale,quality and overall configuration of TCM human resources in Guizhou.Results:The allocation levels of TCM human resources in the northern,western and southwestern Guizhou are higher than the southern and southeast Guizhou.There is autocorrelation in the quality level and overall allocation of TCM human resources.The northern Guizhou shows a high-high agglomeration pattern.The scale and quality of TCM human resources are self-driven,while the quality of TCM human resources has a facilitating effect on the scale of human resources,and this facilitating effect gradually increases over time.Conclusion:It is needed to attach great importance to the improvement of TCM human resource quality;implement the policy of differentiated talent development;and give full play to the diffusion and radiation effect of TCM human resource quality and overall allocation in northern Guizhou.

New quality productivity is a strategic engine for promoting high-quality development and is an inherent requirement and important focus for enhancing new driving forces and building national advantages.The cultivation of innovative talents and technological innovation are key to the development of new quality productivity.Laboratory animal science is a comprehensive interdisciplinary subject that integrates multiple disciplines including biology,medicine,pharmacy,and biomedical engineering.Teaching and research of laboratory animal science not only promotes the creation of innovative talent teams by cultivating innovative consciousness,thinking,spirit,and operational abilities,but also promotes the development of cutting-edge technologies and the transformation of disruptive research result in the fields of basic research and clinical translation of biomedicine,thus providing important guarantees for China's scientific and technological progress and innovative development.

Preeclampsia(PE)is a severe pregnancy complication that poses significant risks to maternal and fetal health,and its pathogenesis remains unclear.Recent studies have shown that lactate dehydrogenase(LDH),a key enzyme in glycolysis,plays a piv-otal role in the development and progression of PE.This article reviews the abnormal changes in the levels of LDH and its isozymes in serum and placental tissue and their impact on the pathogenesis of PE and analyzes the molecular mechanisms by which LDH subunits contribute to placental dysfunction through multiple pathways including hypoxia,inflammation,autophagy dysregulation,and cellular damage.In addition,this article discusses the role of lactate,a metabolic product of LDH activity,in the pathogenesis of PE.LDH can be used as a potential biomarker for PE,and the regulation of non-metabolic functions and metabolic reprogramming mediated by LDH provide new targets for the prevention and treatment of PE.

Objective:To compare the efficacy differences between the combination therapy of Callicarpa nudiflora and dexamethasone versus dexamethasone monotherapy via retention enema in the treatment of acute radiation proctitis.Methods:A total of 100 patients with pelvic malignancies who developed acute radiation proctitis during radiotherapy at Jiangxi Provincial People's Hospital from November 2021 to June 2023 were enrolled,and randomly divided into two groups:a control group(50 cases)vs a treatment group(50 cases).Based on the acute radiation injury scoring criteria established by the Radiation Therapy Oncology Group(RTOG),patients with injury severity scores≤1 were excluded.Subsequently,the remaining patients received the following interventions:the control group(45 cases)underwent single-agent dexamethasone retention enema,while the treatment group(47 cases)received a combined regimen of Callicarpa nudiflora combined with dexamethasone retention enema.Clinical symptoms,defecation frequency,and routine fecal parameters(leukocyte and erythrocyte counts)were recorded before and at 2 weeks of retention enema,to compare the efficacy differences between the two regimens.Results:A statistically significant difference in therapeutic efficacy was observed between the treatment and control groups(χ2=11.37,P=0.003,P<0.05),with the regimen of Callicarpa nudiflora combined with dexamethasone retention enema in the treatment group demonstrating a markedly higher efficacy rate compared to the regimen of single-agent dexamethasone retention enema in the control group(82.2%vs 62.2%).Conclusion:Callicarpa nudiflora combined with dexamethasone retention enema in the treatment of acute radiation proctitis,can significantly alleviates clinical symptoms and improves life quality of patients with acute radiation proctitis.This approach provides novel insights for optimizing clinical treatment strategies for acute radiation proctitis.

Objective:Taking 88 counties in Guizhou from 2021 to 2023 as the research sample,the indicator system of the dimension of Traditional Chinese Medicine(TCM)are constructed to analyze the spatial evolution and spatial effects of the allocation of TCM human resources in Guizhou.Methods:Using spatial correlation model and Vector Autoregression(VAR)model are applied to conduct the spatial autocorrelation test and impulse response study on the scale,quality and overall configuration of TCM human resources in Guizhou.Results:The allocation levels of TCM human resources in the northern,western and southwestern Guizhou are higher than the southern and southeast Guizhou.There is autocorrelation in the quality level and overall allocation of TCM human resources.The northern Guizhou shows a high-high agglomeration pattern.The scale and quality of TCM human resources are self-driven,while the quality of TCM human resources has a facilitating effect on the scale of human resources,and this facilitating effect gradually increases over time.Conclusion:It is needed to attach great importance to the improvement of TCM human resource quality;implement the policy of differentiated talent development;and give full play to the diffusion and radiation effect of TCM human resource quality and overall allocation in northern Guizhou.

Objective:To compare the efficacy differences between the combination therapy of Callicarpa nudiflora and dexamethasone versus dexamethasone monotherapy via retention enema in the treatment of acute radiation proctitis.Methods:A total of 100 patients with pelvic malignancies who developed acute radiation proctitis during radiotherapy at Jiangxi Provincial People's Hospital from November 2021 to June 2023 were enrolled,and randomly divided into two groups:a control group(50 cases)vs a treatment group(50 cases).Based on the acute radiation injury scoring criteria established by the Radiation Therapy Oncology Group(RTOG),patients with injury severity scores≤1 were excluded.Subsequently,the remaining patients received the following interventions:the control group(45 cases)underwent single-agent dexamethasone retention enema,while the treatment group(47 cases)received a combined regimen of Callicarpa nudiflora combined with dexamethasone retention enema.Clinical symptoms,defecation frequency,and routine fecal parameters(leukocyte and erythrocyte counts)were recorded before and at 2 weeks of retention enema,to compare the efficacy differences between the two regimens.Results:A statistically significant difference in therapeutic efficacy was observed between the treatment and control groups(χ2=11.37,P=0.003,P<0.05),with the regimen of Callicarpa nudiflora combined with dexamethasone retention enema in the treatment group demonstrating a markedly higher efficacy rate compared to the regimen of single-agent dexamethasone retention enema in the control group(82.2%vs 62.2%).Conclusion:Callicarpa nudiflora combined with dexamethasone retention enema in the treatment of acute radiation proctitis,can significantly alleviates clinical symptoms and improves life quality of patients with acute radiation proctitis.This approach provides novel insights for optimizing clinical treatment strategies for acute radiation proctitis.

New quality productivity is a strategic engine for promoting high-quality development and is an inherent requirement and important focus for enhancing new driving forces and building national advantages.The cultivation of innovative talents and technological innovation are key to the development of new quality productivity.Laboratory animal science is a comprehensive interdisciplinary subject that integrates multiple disciplines including biology,medicine,pharmacy,and biomedical engineering.Teaching and research of laboratory animal science not only promotes the creation of innovative talent teams by cultivating innovative consciousness,thinking,spirit,and operational abilities,but also promotes the development of cutting-edge technologies and the transformation of disruptive research result in the fields of basic research and clinical translation of biomedicine,thus providing important guarantees for China's scientific and technological progress and innovative development.

Objective:To deepen understanding of IgG 4-related diseases (RDs), we analyzed the associated lymphocyte subtypes, and explored the pathogenesis and potential immunotherapeutic targets. Methods:Eighty-six patients with IgG 4-RDs were enrolled, and their clinical characteristics, peripheral lymphocyte subtypes, and disease course were analyzed. Results:The mean age of the participants was 36-87(62±11) years; 51 were male (59.3%) and 35 were women (40.7%); and 34.9% had a history of allergy. Follow-up lasted 4.8 (0.4, 14.1) months. The most common symptoms were abdominal pain, and submandibular gland and lacrimal gland swelling (each 20.9%). Sixty-five (75.6%) participants had multiple organ involvement, and the most frequently affected organs were the pancreas (52.3%), submandibular gland (51.2%), and lacrimal gland (34.9%). A high eosinophil count; high IgE, IgG, IgG 1, and IgG 4 concentrations; and low complement C3 and C4 concentrations were present in 18.8% (16/85), 30.0% (24/80), 72.9% (62/85), 58.3% (28/48), 89.5% (77/86), 61.2% (52/85), and 50.0% (42/84), respectively, of the participants. In addition, 64.7% (55/85) were positive for autoantibodies, and the most frequent was anti-nuclear antibody (63.5%). The proportion of CD4 +T lymphocytes increased in 25.7% (9/35) of the participants, which was accompanied by an increase in the ratio of CD4 +/CD8 +T lymphocytes (22.9%, 8/35). Importantly, most participants (90.0%, 18/20) had a high proportion of regulatory T (Treg) cells. High interleukin (IL)-2, IL-6, and IL-10 concentrations were present in 50.0% (11/22), 33.3% (10/30), and 16.7% (5/30), respectively, of the participants. Substantial lymphoplasmacytic infiltration, fibrosis, IgG 4-positive plasma cell infiltration, and lymphoid follicle hyperplasia or ectopic formation were present in 79.2% (42/53), 67.9%(36/53), 35.8%(19/53) and 30.2% (16/53), respectively, of the participants. Fifty-three participants with detailed pathologic data were also further evaluated, of whom 24.5% (13/53), 3.8% (2/53), and 67.9% (36/53) had definite, probable, and possible diagnoses; and 3.8% (2/53) could not be diagnosed. Compared with baseline, the percentage of eosinophils and the IgE, IgG, and IgG 4 concentrations decreased significantly; and the complement C3 and C4 concentrations had increased significantly after 6 months of treatment (all P<0.05). The IgG 4 concentration after 6 months of treatment negatively correlated with that of C4, and positively correlated with the baseline concentration of IgE and the IgG 4/IgG ratio. Conclusion:IgG 4-RDs are a group of diseases characterized by male predisposition; multiple organ involvement; a high eosinophil count; high IgE, IgG, IgG 1, and IgG 4 concentrations; and a low C3 concentration. Peripheral CD4 +T cells and Treg cells are also more abundant. The diseases can be controlled with glucocorticoids and immunosuppressive drugs in the majority of instances. The IgG 4 concentration after 6 months of treatment negatively correlates with the baseline complement C4 concentration and positively correlates with the IgE concentration and IgG 4/IgG ratio, which suggests that IgG 4/IgG, IgE, and complement should be closely monitored to evaluate disease activity and the efficacy of treatment in such patients.

Objective To investigate the inhibitory effect and elucidate the molecular mechanism of 6-gingerol on human multiple myeloma cells.Methods The human multiple myeloma cell lines RPMI 8226 and ARH77 were cultured in vitro,followed by treatment with varying concentrations(50,100,200,300,400 μmol/L)of 6-gingerol.The inhibitory effect on cell proliferation was assessed using the CCK-8 assay.Flow cytometry was employed to evaluate cell apoptosis and cycle distribution.Additionally,qRT-PCR and Western blotting techniques were utilized to analyze gene and protein expression levels.Results The proliferation of RPMI 8226 and ARH77 cells was dose-and time-dependently inhibited by 6-gingerol,leading to the induction of apoptosis with statistically significant differences(P＜0.05).Further mechanistic investigations revealed that treatment with 6-gingerol arrested RPMI 8226 cells in the G0/G1 phase,resulting in a significant increase in Bax levels and a decrease in Bcl-2 mRNA and c-Myc mRNA levels(P＜0.05).Additionally,it significantly upregulated the expression of Bax,Cleaved-PARP,Cleaved-caspase3,P53,and p-AKT proteins while down regulating the expression of Bcl-2 protein(P＜0.05).Conclusions The compound 6-Gingerol exhibits inhibitory effects on the proliferation and induction of apoptosis in MM cells,as well as cell cycle arrest at the G0/G1 phase.Its mechanism of action is likely associated with the suppression of the AKT signaling pathway,downregulation of Bcl-2 family protein expression,and inhibition of c-Myc expression.

Objective To investigate the inhibitory effect and elucidate the molecular mechanism of 6-gingerol on human multiple myeloma cells.Methods The human multiple myeloma cell lines RPMI 8226 and ARH77 were cultured in vitro,followed by treatment with varying concentrations(50,100,200,300,400 μmol/L)of 6-gingerol.The inhibitory effect on cell proliferation was assessed using the CCK-8 assay.Flow cytometry was employed to evaluate cell apoptosis and cycle distribution.Additionally,qRT-PCR and Western blotting techniques were utilized to analyze gene and protein expression levels.Results The proliferation of RPMI 8226 and ARH77 cells was dose-and time-dependently inhibited by 6-gingerol,leading to the induction of apoptosis with statistically significant differences(P＜0.05).Further mechanistic investigations revealed that treatment with 6-gingerol arrested RPMI 8226 cells in the G0/G1 phase,resulting in a significant increase in Bax levels and a decrease in Bcl-2 mRNA and c-Myc mRNA levels(P＜0.05).Additionally,it significantly upregulated the expression of Bax,Cleaved-PARP,Cleaved-caspase3,P53,and p-AKT proteins while down regulating the expression of Bcl-2 protein(P＜0.05).Conclusions The compound 6-Gingerol exhibits inhibitory effects on the proliferation and induction of apoptosis in MM cells,as well as cell cycle arrest at the G0/G1 phase.Its mechanism of action is likely associated with the suppression of the AKT signaling pathway,downregulation of Bcl-2 family protein expression,and inhibition of c-Myc expression.