Objective:To investigate the effect of diminished ovarian reserve (DOR) on perinatal outcomes of young patients obtained live births through frozen-thawed embryo transfer (FET) cycles.Methods:In this retrospective cohort study, data of perinatal outcomes of patients who underwent FET and achieved pregnancy ≥24 weeks from January 2006 to December 2018 in Reproductive Medicine Department of Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine were analyzed. Patients were divided into non-DOR group ( n=14 342) and DOR group ( n=1292). The main outcome measure was the rate of low birth weight of newborns. The secondary outcome measures included infant gestational age, birth weight, gender, and incidence of congenital defects, neonatal death, and pregnancy complications. Univariate and multivariate logistic regression were applied to analyze the correlation between DOR and the outcomes of newborns and the rates of pregnancy complications. Results:There was no statistically significant difference in the birth weight of newborns between DOR group and non-DOR group in both singleton and twin pregnancies (all P>0.05). There were no significant differences in incidence of low birth weight, preterm birth, congenital defects, neonatal death, and pregnancy complications between the two groups (all P>0.05). After adjusting for relevant confounders by multivariate logistic regression analysis, DOR was not a risk factor for patients ≤35 years old to obtain singletons and twins with low birth weight through FET (singletons adjusted OR=0.97, 95% CI=0.69-1.34, P=0.831; twins adjusted OR=1.14, 95% CI=0.95-1.36, P=0.166). Conclusion:The birth outcomes of live births obtained by FET in DOR patients ≤35 years old were comparable to those obtained in non-DOR patients.

Objective:To investigate the effect of diminished ovarian reserve (DOR) on perinatal outcomes of young patients obtained live births through frozen-thawed embryo transfer (FET) cycles.Methods:In this retrospective cohort study, data of perinatal outcomes of patients who underwent FET and achieved pregnancy ≥24 weeks from January 2006 to December 2018 in Reproductive Medicine Department of Shanghai Ninth People's Hospital Affiliated Shanghai Jiao Tong University School of Medicine were analyzed. Patients were divided into non-DOR group ( n=14 342) and DOR group ( n=1292). The main outcome measure was the rate of low birth weight of newborns. The secondary outcome measures included infant gestational age, birth weight, gender, and incidence of congenital defects, neonatal death, and pregnancy complications. Univariate and multivariate logistic regression were applied to analyze the correlation between DOR and the outcomes of newborns and the rates of pregnancy complications. Results:There was no statistically significant difference in the birth weight of newborns between DOR group and non-DOR group in both singleton and twin pregnancies (all P>0.05). There were no significant differences in incidence of low birth weight, preterm birth, congenital defects, neonatal death, and pregnancy complications between the two groups (all P>0.05). After adjusting for relevant confounders by multivariate logistic regression analysis, DOR was not a risk factor for patients ≤35 years old to obtain singletons and twins with low birth weight through FET (singletons adjusted OR=0.97, 95% CI=0.69-1.34, P=0.831; twins adjusted OR=1.14, 95% CI=0.95-1.36, P=0.166). Conclusion:The birth outcomes of live births obtained by FET in DOR patients ≤35 years old were comparable to those obtained in non-DOR patients.

Objective:To explore the effects of sulforaphane (SFN), an activator of Nrf2, on anxiety and fear memory in Alzheimer's disease(AD) model mice and mechanism.Methods:The AD mice and wild type (WT) mice with the same background were randomly divided into four groups ( n=12 for each group): wild type + normal saline group (WT+ NS), wild type + sulforaphane (WT+ SFN), AD model + normal saline group (AD+ NS) and AD model + sulforaphane group (AD+ SFN). SFN was dissolved in normal saline (0.9% NaCl) and prepared solution with concentration of 1 g/L.According to body weight, mice in WT+ SFN group and AD+ SFN group were intraperitoneally injected with SFN (10 mg/kg), and mice in WT+ NS group and AD+ NS group were intraperitoneally injected with the same volume of normal saline once a day for 30 days.The open field test was used to detect the autonomous exploration ability and anxious behavior of mice.The elevated cross maze was used to detect the anxiety of mice.Conditional fear test was used to test the fear memory behavior of mice.Finally, the expression of superoxide dismutase(SOD) and malondialdehyde(MDA) in the hippocampus and cerebral cortex were detected by ELISA.Two-way ANOVA analysis was performed using GraphPad Prism 8.0.2 software. Results:In the open field test, the percentage of time in central region in AD+ SFN group ((9.99+ 0.37)%) was higher than that of AD+ NS group ((8.47+ 0.42)%) ( q=3.842, P<0.05). In the elevated cross maze, the percentage of time in open arm of AD+ SFN group ((26.2±1.6)%) was higher than that in AD+ NS group ((15.8±1.0)%) ( q=7.452, P<0.01). In the conditional fear test, all the mice of the four groups developed the fear memory, but AD+ SFN group showed higher freezing time ratio ((64.5±3.8)%) than AD+ NS group ((51.0±4.3)%)( q=5.266, P<0.01) in the testing stage.After SFN intervention, the important indicator of oxidative stress, the expression levels of SOD in hippocampus ( q=6.370, P<0.01) and cortex ( q=7.858, P<0.01) of AD mice increased, while the level of MDA in hippocampus ( q=5.146, P<0.05) and cortex ( q=5.833, P<0.01) decreased. Conclusion:SFN may inhibit oxidative stress through Nrf2 pathway, thereby improving anxiety and fear memory in AD mice.

Objective To investigate the image quality and radiation dose of spectral CT with automatic spectral imaging mode selection (ASIS) and adaptive statistical iterative reconstruction (ASIR) at abdominal CT as compared with low-kVp CT with ASIR.Methods Two hundred patients underwent the plain and arterial-phase (AP) and portal venous-phase (PVP) contrast-enhanced abdominal CT scan were analyzed prospectively.The patients were randomly assigned to the control group (low-kVp CT scan) and study group (spectral CT scan),if BMI ≤ 23.9 kg/m2,patients were assigned into group A (80 kVp) or group B (spectral CT with ASIS);if 24.0 kg/m2 ≤ BMI＜28.9 kg/m2,patients were assigned into group C (100 kVp) or group D (spectral CT with ASIS)(n=50 each).Groups A and B,groups C and D were matched by gender,age,body mass index,cross sectional area and contrast agent dose respectively.Low-kVp images and monochromatic images (40 to 60 keV) were all reconstructed by using ASIR.Radiation dose and quantitative parameters (image noise in HU and contrast-to-noise ratio of the liver,aorta and portal vein) were compared by using t test while overall image quality was assessed by Mann-Whitney U test between the matched groups.Results Radiation dose in the group B was significantly higher than group A (increased by 10％,P＜0.05) but there was no significant differences between groups C and D (P＞0.05).Compared to the control group,image noises in the study group were higher at 40 keV and 50 keV (P＜0.05),but similar at 40 keV (P＞0.05).At the energy level of 40 keV,the study group showed higher CNRs,but lower overall image quality scores than the control group except for the similar image quality scores between groups A and B during AP (P＜0.05).At the energy level of 50 keV,the CNRs and overall image quality scores in the study group were higher than or similar to the control group.At the energy level of 60 keV,the study group showed lower or similar CNRs,but higher or similar overall image quality scores compared with the control group.Conclusions The radiation dose of spectral CT with ASIS technique was slightly higher than 80 kVp CT but similar to 100 kVp CT.By combining ASIR technique,monochromatic image at 50 keV can maintain or improve CNR and overall image quality as compared with low-kVp images.