This document targets digital human metabolic chamber platforms and specifies construction standards and testing protocols covering the full lifecycle of " build-test-operate." It encompasses chamber engineering and environmental control, digital platform and cybersecurity architecture, metabolic measurement and multimodal data acquisition, as well as quantitative system performance and data quality indicators with verifiable acceptance tests. By standardizing architecture, interfaces, and quality control, the specification enables multicenter data interoperability and harmonized quality management, providing high-quality, verifiable, and traceable infrastructure to support precision metabolism research and clinical translation in China.

This document targets digital human metabolic chamber platforms and specifies construction standards and testing protocols covering the full lifecycle of " build-test-operate." It encompasses chamber engineering and environmental control, digital platform and cybersecurity architecture, metabolic measurement and multimodal data acquisition, as well as quantitative system performance and data quality indicators with verifiable acceptance tests. By standardizing architecture, interfaces, and quality control, the specification enables multicenter data interoperability and harmonized quality management, providing high-quality, verifiable, and traceable infrastructure to support precision metabolism research and clinical translation in China.

Objective:To explore the effectiveness of online and offline blended teaching in caregivers of individuals with high-risk pressure injury in primary elderly care institutions.Methods:From December 2020 to December 2021, convenience sampling was used to select 100 caregivers of individuals with high-risk stress injuries from 15 elderly care institutions in cooperation with the Linhai Second People's Hospital as participants. The participants were randomly divided into a control group ( n=50) and an experimental group ( n=50) using the random number method. The control group was treated with usual face-to-face training, and the experimental group was treated with online and offline blended teaching, with an intervention period of three months. The two groups' caring behavior, pressure injury knowledge, and caregiver burden were compared before and after the intervention. Results:After the intervention, the scores of the Pressure Ulcer Caring Behaviors Questionnaire and Caregivers' Knowledge about Pressure Ulcer Questionnaire in the experimental group were higher than those in the control group ( P<0.05). The score of the Zarit Caregiver Burden Interview in the experimental group was lower than that in the control group ( P<0.05) . Conclusions:Online and offline blended teaching is beneficial for improving the knowledge and behavior of caregivers of individuals with high-risk pressure injury in primary elderly care institutions, reducing caregiver burden, and is worth promoting and applying in primary elderly care institutions.

Objective:To explore the effectiveness of online and offline blended teaching in caregivers of individuals with high-risk pressure injury in primary elderly care institutions.Methods:From December 2020 to December 2021, convenience sampling was used to select 100 caregivers of individuals with high-risk stress injuries from 15 elderly care institutions in cooperation with the Linhai Second People's Hospital as participants. The participants were randomly divided into a control group ( n=50) and an experimental group ( n=50) using the random number method. The control group was treated with usual face-to-face training, and the experimental group was treated with online and offline blended teaching, with an intervention period of three months. The two groups' caring behavior, pressure injury knowledge, and caregiver burden were compared before and after the intervention. Results:After the intervention, the scores of the Pressure Ulcer Caring Behaviors Questionnaire and Caregivers' Knowledge about Pressure Ulcer Questionnaire in the experimental group were higher than those in the control group ( P<0.05). The score of the Zarit Caregiver Burden Interview in the experimental group was lower than that in the control group ( P<0.05) . Conclusions:Online and offline blended teaching is beneficial for improving the knowledge and behavior of caregivers of individuals with high-risk pressure injury in primary elderly care institutions, reducing caregiver burden, and is worth promoting and applying in primary elderly care institutions.

COVID-19 pandemic caused by SARS-CoV-2 infection severely threatens global health and economic development. No effective antiviral drug is currently available to treat COVID-19 and any other human coronavirus infections. We report herein that a macrolide antibiotic, carrimycin, potently inhibited the cytopathic effects (CPE) and reduced the levels of viral protein and RNA in multiple cell types infected by human coronavirus 229E, OC43, and SARS-CoV-2. Time-of-addition and pseudotype virus infection studies indicated that carrimycin inhibited one or multiple post-entry replication events of human coronavirus infection. In support of this notion, metabolic labelling studies showed that carrimycin significantly inhibited the synthesis of viral RNA. Our studies thus strongly suggest that carrimycin is an antiviral agent against a broad-spectrum of human coronaviruses and its therapeutic efficacy to COVID-19 is currently under clinical investigation.

OBJECTIVE: To assess the effect of transnasal humidified rapid-insufflation ventilatory exchange (THRIVE) on gastric insufflation during general anesthesia induction in obese patients. METHODS: Ninety obese patients (BMI 30-39.9 kg/m RESULTS: The incidence of gastric insufflation was significantly higher in Group M and Group M+T than in Group T ( CONCLUSIONS Ultrasound monitoring of the comet tail sign and the changes of CSA-GA in the gastric antrum is feasible and reliable for detecting gastrointestinal airflow, and in obese patients, the application of THRIVE for induction of anesthesia can ensure the oxygenation level without further increasing gastric insufflation.
Anesthesia, General ; Humans ; Insufflation ; Intubation, Intratracheal ; Masks ; Obesity

In this study, models for assessing resources of forestry residues were improved based on analysis of previous studies and current forest production in China. And novelties are highlighted: this study covered the models for the calculation of 10 secondary forestry residues from woody nursery, forest woody pruning, wood logging, firewood, wood bucking, wood handling, waste wood, banana and pineapple plants, bamboo processing and waste bamboo; the residues produced from fast-growing forest for logging and processing of imported wood were taken into consideration to calculate woody residue potential; the calculation of pruning residue included woody fruit trees, and herbaceous fruit plants from the management of orchard and other economic forest; the consistency of parameter terminology and definition which referred from their literature resources have been ensured; more studies focused on the coefficient values, industrial standard, forestry residue amount and spatial distribution are recommended for future researches.

Objective To compare effectiveness,performance,onset time and complications between ultrasound-guided cervical transverse process block and interscalene brachial plexus block in patients undergoing proximal humeral surgeries.Methods Sixty patients,27 males and 33 females, aged 18-70 years,scheduled for proximal humeral surgeries were randomly divided into two groups. They were given either cervical transverse process block (group T,n =30)or ultrasound-guided in-terscalene brachial plexus block (group I,n =30).All patients received a total of 8 ml of 0.5% ropiv-acaine.The performance, anesthetic onset time, the side effects and block success rate were evaluated.Results Block procedure was quicker in group T than in group I [(8.73 ±3.1 7)min vs. (14.40±8.21)min,P <0.01].The severity of diaphragmatic paralysis in group T was significantly lower than in group I (P <0.01).The ultrasound-guided cervical transverse process block was more effective than the interscalene brachial plexus block in patients undergoing proximal humeral surgeries (100% vs.80%,P <0.05).Conclusion The ultrasound-guided cervical transverse process block has a higher success rate and fewer incidence of diaphragmatic paralysis than the interscalene brachial plexus block in patients undergoing proximal humeral surgeries.

Objective To evaluate the effect of lovastatin on shedding of heparan sulfate proteoglycan (HSPG) and syndecan-1 (SDC-1) in the lung tissues of rats with sepsis-induced acute lung injury.Methods One-hundred and twenty male Wistar rats aged 8-12 weeks,weighing 325-425 g,were randomly divided into 3 groups (n =40 each) using a random number table:sham operation group (group S),cecal ligation and puncture (CLP) group and lovastatin group (group L).Lovastatin 4 mg/kg was injected once a day for 5 consecutive days in S and L groups,while the equal volume of 0.5％ CMC (the solvent) was given in CLP group.Sepsis was produced by CLP on 5th day of administration in CLP and L groups.The left lung was lavaged at 24 h after operation.The broncho-alveolar lavage fluid (BALF) was collected for determination of protein concentrations,white blood cell (WBC) count and percentage of neutrophils.Blood samples were collected for determination of the concentrations of HSPG and SDC-1 in serum (by ELISA).Evans blue was injected at 24 h after operation in the remaining 20 rats of each group.The lungs were removed for examination of the pathological changes and for measurement of HSPG and SDC-1 mRNA and protein expression (using Western blot and PCR),and Evans blue content (reflecting pulmonary capillary permeability) in the lung tissue.Results Compared with group S,the protein concentrations,WBC count and percentage of neutrophils in BALF,Evans blue content in lung tissues and the concentrations of HSPG and SDC-1 in serum were significantly increased,and HSPG and SDC-1 mRNA and protein expression was down-regulated in CLP and L groups.Compared with group CLP,the protein concentrations,WBC count and percentage of neutrophils in BALF,Evans blue content in lung tissues and the concentrations of HSPG and SDC-1 in serum were significantly decreased,and HSPG and SDC-1 mRNA and protein expression was up-regulated in group L.The pathological changes of lungs were significantly attenuated in group L as compared with group CLP.Conclusion The mechanism by which lovastatin attenuates acute lung injury induced by sepsis may be related to reduced shedding of HSPG and SDC-1 in lung tissues and improved function of pulmonary vascular endothelium in rats.