Acute ischemic stroke (AIS) is a cerebrovascular disease characterized by high morbidity, disability, and mortality, posing a significant threat to human health. Endovascular treatment has now been established as a key method for AIS management, in which stent retrievers that can mechanically remove blood clots play a key role in this technique. In recent years, stent retrievers have evolved in complexity and functionality to improve the ability of clot removing and surgical safety. However, the present instruments still have limitations on treatment efficiency, vascular adaptability, and operational precision, posing an urgent need for innovation in the design of stent retrievers. This paper systematically reviewed the structural features and working principles of AIS stent retrievers from the perspective of efficacy evaluation metrics, historical development, recent advancements in stent retrieval technology, and future prospects.
Humans ; Ischemic Stroke/surgery* ; Stents ; Endovascular Procedures/methods* ; Thrombectomy/methods* ; Device Removal/methods*

Objective:To explore the effects of Sangqiao Qingfei Prescription combined with Western medicine conventional therapy with mechanical ventilation on lung function and airway inflammation in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) respiratory failure; To evaluate clinical efficacy.Methods:A randomized controlled trial study was conducted. Totally 90 AECOPD patients with respiratory failure in our hospital from January 2020 to December 2022 were selected as the observation subjects. They were divided into two groups using a random number table method, with 45 cases in each group. The control group received mechanical ventilation treatment, while the observation group received Sangqiao Qingfei Prescription on the basis of the control group. Both groups were treated for 2 weeks. TCM syndrome scoring was performed before and after treatment, and the time of successful withdrawal from the machine was recorded; a blood gas analyzer was used to detect PaO 2, PaCO 2, blood oxygen saturation (SaO 2) and pH values; the plateau pressure (Pplat), peak airway pressure (Ppeak), airway resistance (Raw) and dynamic lung compliance (Cdyn) were recorded during the ventilator; a pulmonary function meter was used to measure respiratory rate (RR), maximum expiratory flow (PEF), FVC, FEV1, and the percentage of FEV1 to the estimated value (FEV1% estimated value); serum CRP, TNF-α, and Procalcitonin (PCT) were detected using ELISA method. Clinical efficacy was evaluated. Results:During the treatment period, there were no cases of detachment in both groups. The mechanical ventilation time in the observation group was (7.16 ± 0.69) d, while in the control group it was (9.88 ± 1.04) d, with statistical significance ( t=14.62, P<0.001); after treatment, the main symptom, secondary symptom scores, and total scores of the observation group were lower than those in the control group ( t values of 13.43, 18.53, 31.21, P<0.001); the PaO 2 [(79.16 ± 7.42) mmHg vs. (67.49 ± 6.88) mmHg, t=8.24], SaO 2 [(95.15 ± 9.93)% vs. (84.59 ± 9.48)%, t=5.16], and pH value (7.35 ± 0.23 vs. 7.26 ± 0.16, t=2.16) in the observation group were higher than those in the control group ( P<0.01 or P<0.05), while PaCO 2 [(49.89 ± 3.65) mmHg vs. (62.39 ± 4.27) mmHg, t=14.93] was lower than that of the control group ( P<0.01); after treatment in the observation group, Pplat [(15.31 ± 2.51) cmH 2O vs. (17.53 ± 2.02) cmH 2O, t=4.62], Ppeak [(22.43 ± 3.16) cmH 2O vs. (25.78 ± 3.17) cmH 2O, t=5.02], Raw [(18.96 ± 3.86) cmH 2O/(S?L) vs. (24.29 ± 4.29) cmH 2O/(S?L), t=6.20] were lower than those in the control group ( P<0.01), Cdyn [(34.53 ± 3.35) cmH 2O/(S?L) vs. (30.27 ± 3.87) cmH 2O/(S?L), t=5.58] was higher than the control group ( P<0.01); the RR [(19.25 ± 2.43) times/min vs. (23.49 ± 3.07) times/min, t=7.26] in the observation group was lower than that of the control group ( P<0.01), PEF [(4.99 ± 0.40) L/s vs. (4.03 ± 0.34) L/s, t=12.27], FVC [(3.04 ± 0.20) L vs. (2.14 ± 0.22) L, t=20.31], FEV1 [(2.83 ± 0.20) L vs. (2.16 ± 0.13) L, t=18.84], FEV1% estimated value [(42.23 ± 4.66)% vs. (36.43 ± 5.09)%, t=5.64] were higher than those in the control group ( P<0.01); serum CRP, IL-6, TNF-α and PCT in the observation group were lower than those in the control group ( t values were 18.13, 13.36, 15.97, 30.67, P<0.01). The total effective rate of the observation group was 93.33% (42/45), while that of the control group was 77.78% (35/45), with statistical significance ( χ2=4.41, P=0.036). Conclusion:The combination of Sangqiao Qingfei Prescription and conventional Western medicine treatment with mechanical ventilation can effectively improve lung ventilation function, reduce inflammatory cytokine levels, alleviate inflammatory reactions, and improve clinical efficacy in AECOPD patients with respiratory failure.

Objective: To construct the mutants of ARID1A gene, which is an important component in human chromosomal remodeling complex Switch/Sucrose Non Fermentable (SWI/SNF), and identify their overexpression in liver cancer HepG2 cells. Methods: Overlap PCR was used to construct domain truncated mutantss pcDNA6-ARID1A/ΔARID and pcDNA6-ARID1A/ΔD UF3518 based on wild type plasmids pcDNA6-ARID1A. Lipofectionmethod was used to transfect the wild type and mutants into HepG2. Real-time PCR and western blotting were used to confirm the overexpression of ARID1A and the mutants. Results: SDS-PAGE and sequencingresult confirmed the successful construction of pcDNA6-ARID1A/ΔARID and pcDNA6-ARID1A/ΔDUF3518. Real-time PCR and western blottingresult confirmed the overexpression of both mRNA and protein of wild type ARID1A and ARID1A/ΔARID. The mRNA levels indicated that ARID1A/Δ DUF3518 were overexpressed, but the protein levels were quite low. Conclusions Functional domain truncated mutants of ARID1A were successfully constructed. Overexpression of wild type ARID1A and ARID1A/ΔARID in liver cancer HepG2 cells was successful. Loss of ARID1A/ΔDUF3518 protein suggest that DUF3518 may contribute to the protein structure stability.