Objective To explore the regulatory effect of long non-coding RNA HOX transcript anti-sense RNA(lnRNA HOTAIR)targeting tuberous sclerosis complex 1(TSC1)on podocyte injury in diabetic nephropathy(DN).Methods Mice podocyte MPC5 were divided into 6 groups:the control group,the high glucose group(the HG group),the HG+si-NC group,the HG+si-HOTAIR group,the HG+si-HOTAIR+sh-NC group,and the HG+si-HOTAIR+sh-TSC1 group.qPCR was used to detect the levels of lnRNA HO-TAIR and TSC1 mRNA.Cell viability was detected by cell counting kit-8(CCK-8),and apoptosis was detec-ted by flow cytometry.ELISA was used to detect the levels of inflammatory factors[IL-6,tumor necrosis fac-tor-α(TNF-α)]and the levels of oxidative stress indicators[reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD)]were detected by corresponding reagent kit.Results Compared with the control group,the levels of lnRNA HOTAIR,apoptosis rate,IL-6,TNF-α,ROS and MDA in the HG group were significantly increased(P<0.05),while the levels of TSC1 mRNA,cell viability and SOD were significantly decreased(P<0.05).Compared with the HG+si-NC group,the levels of lnRNA HOTAIR,ap-optosis rate,IL-6,TNF-α,ROS and MDA in the HG+si-HOTAIR group significantly decreased(P<0.05),the levels of TSC1 mRNA,cell viability and SOD were significant increased(P<0.05).Compared with the HG+si-HOTAIR+sh-NC group,the levels of apoptosis rate,IL-6,TNF-α,ROS and MDA in the HG+si-HOTAIR+sh-TSC1 group were significantly increased(P<0.05),the levels of TSC1 mRNA,cell viability and SOD were significantly decreased(P<0.05).Conclusion Silencing lnRNA HOTAIR can target TSC1 to regulate the activity,apoptosis rate,inflammatory level and oxidative stress levels of podocyte injury in DN,thereby alleviating podocyte damage in DN.

Objective:To analyze sentinel symptoms in gastric cancer chemotherapy patients based on symptom cluster theory and provide a basis for effective symptom assessment and management.Methods:A convenience sampling method was used to select gastric cancer patients undergoing chemotherapy at the First Affiliated Hospital of Zhengzhou University from June 2022 to May 2023. Patients were surveyed using a basic demographic questionnaire and the M.D. Anderson Symptom Inventory (MDASI). The Apriori algorithm was applied to identify sentinel symptoms within symptom clusters during chemotherapy.Results:A total of 195 questionnaires were distributed, and 187 valid responses were collected, yielding a response rate of 95.90% (187/195). A total of four symptom clusters were identified: physical symptom cluster (fatigue-pain-restless sleep-drowsiness-weight loss), gastrointestinal symptom cluster (nausea-appetite loss-dry mouth-vomiting), emotional symptom cluster (sadness-distress), and gastric cancer-specific symptom cluster (feeling full-changed appetite-diarrhea-constipation). Fatigue, nausea, sadness, and feeling full were identified as sentinel symptoms for the physical, gastrointestinal, emotional, and gastric cancer-specific symptom clusters, respectively.Conclusions:Gastric cancer chemotherapy patients experience various symptom clusters, with fatigue, nausea, sadness, and feeling full being potential sentinel symptoms within their respective clusters.

Objective:To analyze sentinel symptoms in gastric cancer chemotherapy patients based on symptom cluster theory and provide a basis for effective symptom assessment and management.Methods:A convenience sampling method was used to select gastric cancer patients undergoing chemotherapy at the First Affiliated Hospital of Zhengzhou University from June 2022 to May 2023. Patients were surveyed using a basic demographic questionnaire and the M.D. Anderson Symptom Inventory (MDASI). The Apriori algorithm was applied to identify sentinel symptoms within symptom clusters during chemotherapy.Results:A total of 195 questionnaires were distributed, and 187 valid responses were collected, yielding a response rate of 95.90% (187/195). A total of four symptom clusters were identified: physical symptom cluster (fatigue-pain-restless sleep-drowsiness-weight loss), gastrointestinal symptom cluster (nausea-appetite loss-dry mouth-vomiting), emotional symptom cluster (sadness-distress), and gastric cancer-specific symptom cluster (feeling full-changed appetite-diarrhea-constipation). Fatigue, nausea, sadness, and feeling full were identified as sentinel symptoms for the physical, gastrointestinal, emotional, and gastric cancer-specific symptom clusters, respectively.Conclusions:Gastric cancer chemotherapy patients experience various symptom clusters, with fatigue, nausea, sadness, and feeling full being potential sentinel symptoms within their respective clusters.

Objective:To investigate the clinical efficacy of 3D printing -assisted percutaneous transforaminal endoscopic discectomy (PTED) in the treatment of lumbar disc herniation (LDH). Methods:A randomized controlled trial was conducted involving 60 patients with LDH who received treatment at The Second Affiliated Hospital of Bengbu Medical University from January 2020 to June 2023. The patients were randomly assigned to two groups using the random number table method, with 30 patients in each group. The control group received conventional PTED, while the observation group underwent 3D printing -assisted PTED. Surgical indicators, including operation time, number of fluoroscopy sessions, and number of needle punctures, as well as clinical efficacy assessed at 3 months postoperatively, were compared between the two groups. Results:The observation group had a significantly lower number of punctures [(2.17 ± 0.68) times] compared with the control group [(5.01 ± 2.15) times]. The operation time in the observation group [(64.31 ± 8.33) minutes] was significantly shorter than that in the control group [(71.89 ± 8.72) minutes]. The observation group had fewer fluoroscopy sessions [(12.27 ± 2.61) times] compared with the control group [(19.20 ± 2.67) times]. All differences were statistically significant ( t = 11.90, 3.63, 10.16, all P < 0.05). At 3 months postoperatively, the rates of excellent and good clinical outcomes according to the MacNab criteria were 96.67% (29/30) in the observation group and 93.33% (28/30) in the control group, with no statistically significant difference between the two groups (χ2 = 0.00, P = 1.000). Conclusion:Preoperative planning utilizing 3D printing technology can effectively assist with the puncture during PTED. It can enhance the efficiency and safety of the procedure, reduce radiation exposure, and exhibit significant application value.

Objective:To investigate the clinical efficacy of 3D printing -assisted percutaneous transforaminal endoscopic discectomy (PTED) in the treatment of lumbar disc herniation (LDH). Methods:A randomized controlled trial was conducted involving 60 patients with LDH who received treatment at The Second Affiliated Hospital of Bengbu Medical University from January 2020 to June 2023. The patients were randomly assigned to two groups using the random number table method, with 30 patients in each group. The control group received conventional PTED, while the observation group underwent 3D printing -assisted PTED. Surgical indicators, including operation time, number of fluoroscopy sessions, and number of needle punctures, as well as clinical efficacy assessed at 3 months postoperatively, were compared between the two groups. Results:The observation group had a significantly lower number of punctures [(2.17 ± 0.68) times] compared with the control group [(5.01 ± 2.15) times]. The operation time in the observation group [(64.31 ± 8.33) minutes] was significantly shorter than that in the control group [(71.89 ± 8.72) minutes]. The observation group had fewer fluoroscopy sessions [(12.27 ± 2.61) times] compared with the control group [(19.20 ± 2.67) times]. All differences were statistically significant ( t = 11.90, 3.63, 10.16, all P < 0.05). At 3 months postoperatively, the rates of excellent and good clinical outcomes according to the MacNab criteria were 96.67% (29/30) in the observation group and 93.33% (28/30) in the control group, with no statistically significant difference between the two groups (χ2 = 0.00, P = 1.000). Conclusion:Preoperative planning utilizing 3D printing technology can effectively assist with the puncture during PTED. It can enhance the efficiency and safety of the procedure, reduce radiation exposure, and exhibit significant application value.

Objective:To explore the effect of remote management based on patient-reported outcomes (PROs) monitoring in patients with breast cancer undergoing chemotherapy.Methods:From September 2020 to March 2021, a total of 134 patients with breast cancer undergoing chemotherapy in the First Affiliated Hospital of Zhengzhou University were selected as research objects by the convenient sampling method, and were divided into the control group and the observation group using the random number table method, with 67 cases in each group. The control group received routine care, while the observation group implemented remote management based on PROs monitoring on the basis of the control group, lasting for 3 months. Quality of life and psychological distress were compared between the two groups.Results:After intervention, the total scores and scores of all dimensions of quality of life of the observation group were higher than those of the control group, and the differences were statistically significant ( P<0.05). After intervention, the scores of psychological distress degree of the observation group was lower than that of the control group, and the number of patients with psychological distress score greater than 4 points was less than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Remote management based on PROs monitoring can improve quality of life of patients, alleviate their psychological distress, which is worthy of clinical promotion and application.

Anti-CD19 chimeric antigen receptor (CAR)-T cell therapy has achieved 40%-50% long-term complete response in relapsed or refractory diffuse large B-cell lymphoma (DLBCL) patients. However, the underlying mechanism of alterations in the tumor microenvironments resulting in CAR-T cell therapy failure needs further investigation. A multi-center phase I/II trial of anti-CD19 CD28z CAR-T (FKC876, ChiCTR1800019661) was conducted. Among 22 evaluable DLBCL patients, seven achieved complete remission, 10 experienced partial remissions, while four had stable disease by day 29. Single-cell RNA sequencing results were obtained from core needle biopsy tumor samples collected from long-term complete remission and early-progressed patients, and compared at different stages of treatment. M2-subtype macrophages were significantly involved in both in vivo and in vitro anti-tumor functions of CAR-T cells, leading to CAR-T cell therapy failure and disease progression in DLBCL. Immunosuppressive tumor microenvironments persisted before CAR-T cell therapy, during both cell expansion and disease progression, which could not be altered by infiltrating CAR-T cells. Aberrant metabolism profile of M2-subtype macrophages and those of dysfunctional T cells also contributed to the immunosuppressive tumor microenvironments. Thus, our findings provided a clinical rationale for targeting tumor microenvironments and reprogramming immune cell metabolism as effective therapeutic strategies to prevent lymphoma relapse in future designs of CAR-T cell therapy.

A case of limb girdle muscular dystrophy type 2S (LGMD2S) caused by maternal uniparental disomy on chromosome 4 at the First Affiliated Hospital of Henan University of Chinese Medicine in March 2020 was reported.The female child, aged 9 months and 4 days, presented with developmental delay after bacterial meningitis in early infancy, decreased muscle strength in infancy and increased muscle and liver enzymes.Family genetic analysis showed that the child′s monodiploid in chromosome 4 was maternal origin, and the homozygous c. 1066T > G (p.Y356D) of TRAPPC11 gene may had pathogenic variation, which came from the child′s mother.The final diagnosis of LGMD2S was made according to the clinical manifestations and gene test results.LGMD2S is a rare autosomal recessive disease caused by the pathogenic variation of TRAPPC11 gene.Its clinical characteristics include proximal limb weakness, motor and intellectual retardation, seizures, motor disorders, elevated serum creatine kinase and muscular dystrophy like pathological changes in children.