Obesity, as a chronic recurrent disease, has become a major public health challenge in China. To implement the requirements of the Healthy China Initiative (2019—2030), under domestic guidelines or consensus statements on overweight and obesity, and in alignment with the latest scientific advances globally, the Quality control protocol for adult overweight and obesity screening in health management (examination) institutions (2025 edition) was developed. This protocol was drafted by the Health Management Center of Shanghai Changzheng Hospital and formulated through multiple rounds of deliberation by experts in China’s health examination quality control field. The protocol establishes unified standards for screening facilities, personnel qualifications, and measurement or testing procedures. It defines specific screening items, outlines a standardized screening pathway, and sets requirements for the final medical review, ensuring the scientific validity, effectiveness, and safety of the screening process. The implementation of this protocol will enhance the consistency of weight management practices for adults across health examination institutions and strengthen the quality control of overweight and obesity screening programs.

Objective:To dynamically trace the migration and therapeutic effects of human bone marrow mesenchymal stem cells (MSCs) in mice with liver injury after cell transplantation through in vivo bioluminescent imaging (BLI).Methods:The MSCs were transfected with the lentivirus CMV-Luciferase2-mKate2 and mKate2 positive cells were purified and screened by fluorescence-activated cell sorting (FACS) after 96 h. The purified MSCs-R (MSCs-CMV-Luciferase2-mKate2) were used by in vitro and in vivo BLI. The mice (male BALB/c nude mice) were divided into 4 groups with 9 mice per group by random number table method, including (1) Liver injury experimental group: The liver injury model was established by intraperitoneal injection of CCl 4, and MSCS-R transplantation through spleen injection was performed 24 h later; (2) Control experimental group: The same volume of phosphate buffer (PBS) was injected intraperitoneally, and MSCS-R transplantation through spleen injection was performed 24 h later; (3) Liver injury group: Liver injury model was established and PBS was injected into the spleen;(4) Blank group: The mice were intraperitoneally injected of PBS.BLI was performed daily after cell transplantation until light signals disappeared in the liver region, and the pathological examination of liver tissue was obtained 14 d after MSCs-R transplantation. Linear regression analyses were performed to determine the correlation between the optical signal intensity and the number of cells, and statistical differences of the optical signal intensity between liver injury experimental group and control experimental group were evaluated using the Student′s t test. Results:The MSCs were readily transfected with lentivirus CMV-Luciferase2-mKate2 for 96 h. The transfected MSCs were purified by FACS and more than 95% of MSCs were mKate2 positive. The optical signal intensity of MSCs-R detected by BLI in vitro significantly correlated with cell numbers in vitro (R 2=0.980). In both of liver injury experimental group and control experimental group, cell migration to the liver was observed on the first day after intrasplenic transplantation of MSCs-R, and the optical signal intensity in the area of liver of liver injury experimental group was higher than that of control experimental group ( t=15.476, P<0.001). The optical signal intensity in the hepatic area was observed in 11 d after transplantation in liver injury experimental group, compared to control experimental group in 5 d. Optical signal was not detected in mice in the other two groups. Histopathology showed that the degree of liver injury after MSCs-R transplantation was significantly lower in liver injury experimental group than control experimental group. Conclusions:The dynamical migration of MSCs transplanted to the spleen and settled in the damaged liver could be tracked by BLI, and liver injury can prompt MSCs directionally migrate to the damaged tissues and play their role in repairing liver injury.

Objective The purpose of this study was to evaluate whether whole-tumor histogram-based analysis of readout-segmented echo-planar diffusion-weighted imaging (RESOLVE-DWI) ADC map can help in the discrimination of parotid gland tumors(pleomorphic adenoma, Warthin tumor, malignant parotid gland tumor). Methods The MR images(pre / post-contrast enhanced MRI, RESOLVE-DWI, ADC map) of 47 patients with a biopsy-or surgery-proven pleomorphic adenomas, 25 patients with Warthin tumors and 36 patients with malignant parotid gland tumors were retrospectively analyzed. Histogram-based analysis was performed with the software MaZda. ROIs were drawn on every section of the ADC map containing the tumor, then 12 Parameters(Area, MinNorm, MaxNorm, Mean,Variance, Skewness, Kurtosis, Perc.1％, Perc.10％, Perc.50％, Perc.90％, Perc.99％) derived from histogram were calculated. Statistical analysis among the three groups (One-way ANOVA or Kruskal-Wallis test) were performed to find out the statistical significance of each histogram parameter. Then LSD test or Mann-Whitney U test was used for pairwise comparison between groups. And the differential efficiency of each parameter was determined using ROC analysis. Results Overall, 9 parameters (MinNorm, MaxNorm, Mean, Variance, Skewness, Perc. 10％, Perc. 50％, Perc. 90％, Perc. 99％) among three groups were shown to be statistically significant (P<0.05). Between the pleomorphic adenomas and Warthin tumors, these 9 parameters were of statistical significance. Perc.50％revealed the highest diagnostic efficiency, followed by Mean and Perc.10％. Between the pleomorphic adenomas and malignant parotid gland tumors, also all these 9 parameters were of statistical significance. Mean was revealed to have the highest diagnostic efficiency, followed by Perc. 10％ and Perc. 90％. However, between the Warthin tumors and malignant parotid gland tumors, only 5 parameters (MinNorm, Mean, Skewness, Perc. 10％, Perc. 50％) were statistically significant. MinNorm was revealed to have the highest diagnostic efficiency, followed by Perc. 50％ and Perc. 10％. Generally, Mean, Perc. 10％ and Perc. 50％ were more effective in the differential diagnosis of these three types common parotid neoplasms. Conclusion Whole-tumor histogram analysis of ADC maps are effective in differentiating common parotid neoplasms.

Objective To investigate the status quo of the off-label use in patients with major mental disorders in a tertiary psychiatric hospital and to analyze its relevant risk factors,so as to provide the basis for establishing the off-label use norm.Methods The data of inpatients with major mental disorders (schizophrenia,bipolar disorder and depression) were collected from electronic medical records system in the Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital).The incidence and categories of off-label use were analyzed by descriptive analysis.The chi-square test and logistic regression analysis were used to analyze factors such as age,gender,diagnosis,the number of prescribed psychotropic drugs,physician title,etc.Results A total of 2 371 patients and 10 794 prescriptions were collected and analyzed.The incidence of off-label use was 80.43％ (1 907/2 371) and 42.46％ (4 583/10 794) in patients and prescriptions,respectively.The main categories and constituent ratio of off-label use were indications (89.16％,4 086/4 583),dosage (4.63％,212/4 583),population and age (0.31％,14/4 583),while there were 5.91％ (271/4 583) prescriptions contain 2 or more different categories.The off-label use was widespread among different types of psychotropic drugs,mental disorders and clinical departments.The risk factors were the number of prescribed psychotropic drugs (Wald=177.218,P＜0.01),the diagnosis of mental disorders (Wald=35.320,P＜0.01) and physician title (Wald=8.667,P=0.003).Conclusion Off-label use is common in patients in the tertiary psychiatric hospital,involving different kinds of psychotropic drugs,mental disorders and clinical departments.The primary category of off-label use is indications.

Objective To investigate the status quo of the off-label use in patients with major mental disorders in a tertiary psychiatric hospital and to analyze its relevant risk factors,so as to provide the basis for establishing the off-label use norm.Methods The data of inpatients with major mental disorders (schizophrenia,bipolar disorder and depression) were collected from electronic medical records system in the Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital).The incidence and categories of off-label use were analyzed by descriptive analysis.The chi-square test and logistic regression analysis were used to analyze factors such as age,gender,diagnosis,the number of prescribed psychotropic drugs,physician title,etc.Results A total of 2 371 patients and 10 794 prescriptions were collected and analyzed.The incidence of off-label use was 80.43％ (1 907/2 371) and 42.46％ (4 583/10 794) in patients and prescriptions,respectively.The main categories and constituent ratio of off-label use were indications (89.16％,4 086/4 583),dosage (4.63％,212/4 583),population and age (0.31％,14/4 583),while there were 5.91％ (271/4 583) prescriptions contain 2 or more different categories.The off-label use was widespread among different types of psychotropic drugs,mental disorders and clinical departments.The risk factors were the number of prescribed psychotropic drugs (Wald=177.218,P＜0.01),the diagnosis of mental disorders (Wald=35.320,P＜0.01) and physician title (Wald=8.667,P=0.003).Conclusion Off-label use is common in patients in the tertiary psychiatric hospital,involving different kinds of psychotropic drugs,mental disorders and clinical departments.The primary category of off-label use is indications.

BACKGROUND:There are stil some controversies about lateral compression rotational y unstable pelvic ring injuries and fixed mode. OBJECTIVE:To evaluate the pelvic stability of locking plate fixation pelvic reconstruction repairing lateral compression rotational y unstable pelvic ring injuries. METHODS:We retrospectively analyzed 15 patients with unstable lateral compression rotational y unstable pelvic ring injuries treated with reconstruction locking plates fixation at Suzhou Xiangcheng People’s Hospital between November 2011 and November 2014. The Matta standard was used to evaluate the quality of fracture reduction. Clinical efficacy was evaluated by Majeed score. RESULTS AND CONCLUSION:Fifteen patients were fol owed up for 12-44 months (mean 24 months). According to the standard of Matta, 11 cases were excel ent, 2 cases were good, 2 cases were average, 0 case poor, and the excel ent and good rate was 87%. According to Majeed functional assessment, clinical function outcomes were graded as 9 cases excel ent, 3 cases good, 1 case average, 1 case poor;excel ent and good rate was 80%. There were no patient loss of reduction and internal fixation failure among these 15 patients in the final fol ow-up. No iatrogenic neurovascular injury occurred. Incision superficial infection was detected in 2 patients with a Morel-Laval ee lesion after subjecting to double-tube continuous negative pressure drainage, and healed after wound management. A large area of infection and skin necrosis did not occur. These results confirm that pelvic universal reconstruction locking plates in repair of lateral compression rotational y unstable pelvic ring injuries can maintain a strong and effective fixation. We should pay much attention to the cases of pelvic fractures combined with soft tissue injury around the pelvis.

Objective To improve the recognition and management of primary breast lymphoma(PBL) by discussing the clinical pathological characteristics and the prognosis of PBL. Methods The clinical and treatment records of PBL pa-tients admitted in Boai hospital of zhongshan from January 2001 to December 2012 were analyzed. Results All cases were B-cell origin(25/25),61.5%(16/25)were diffuse large B-cell lymphoma origin,The 5-year overall survival was 44%. There were no significant difference in the 5-year overall survival rates, 5-year local control rates and 5-year distant control rates in patient treatment with any surgery compared with no-Surgery(P>0.05).There were no significant difference in the 5-year overall survival rates and 5-year distant control rates in patient treatment with any RT com-pared with no-RT(P>0.05),but the 5-year local control rates was significantly different(P<0.05);There were no sig-nificant difference in the 5-year overall survival rates and 5-year local control rates in patient treatment with any chemotherapy compared with no-chemotherapy (P>0.05). but the 5-year distant control rates was significant different(P<0.05). Conclusion The prognosis of PBL is related with the clinical stage and pathological type. Radiotherapy and chemotherapy respectively promote the local control and distant control. The optimal treatment is surgery combined with chemotherapy and/or radiotherapy.

Objective To renovate angiography in identifying portal vein anatomy during transjugular intrahepatic portosystemic shunt (TIPS) procedures,saving the time of TIPS procedures,decreasing the risk of the complications of the post-procedure.MethodsThe difference between the Wedge hepatic venography with Carbon Dioxide in 6 cases and Inferior Mesenteric artery angiography in 7 cases during TIPS procedures were compared in the identification of portal vein anatomy.The quality of images,their effects on the procedures,the complications and the recovery post-procedure were evaluated.Results Using CO2,the portal veins were opacified in all 6 cases.TIPS procedures succeeded in all cases except 1 case because of poor coagulation function.Using Inferior Mesenteric artery angiography,the portal veins were opacified in al1 7 cases.TIPS procedure succeeded in all cases except 1 case because of chronic portal occlusion.Puncture-site hematoma occurred in 1 case after TIPS procedure.ConclusionWedge hepatic venography with Carbon Dioxide is superior,safer and more convenient than Inferior Mesenteric Artery angiography in identifying portal vein anatomy during TIPS.

Objective To evaluate the diagnostic value of multi-slice three-dimensional computed tomographic angiography(MS 3D-CTA)for vertebrobasilar dolichoectasia(VBD).Methods MS 3D-CTA of 10 patients with VBD were retrospectively analysed.Source images were got by GE Lightspeed pro scanner.Volume rendering(VR)and maximum intensity project (MIP) were adopted to reconstruct 3D images in all cases.Twenty patients were selected as the control group by suspected cerebra[vascular diseases and underwent MS 3D-CTA at the same period.Enumeration data between the patient group and the control group was assessed by Wilcoxon.test.Results There were 2 types of 10 cases with VBD,including simple type(n=4)and saddle type(n=6).Compared with the control group of the length of the basilar artery(B 1,25.60 mm),the deviant degree(Bc,1.20 mm),the height(Bh,1.90 am),the length of the vertebral artery (V1,17.55 mm),the deviant degree(Vc,2.05 mm),and the diameter of BA and/or VA (Bw/Vw,3.05 mm),there is significant difference in the B1 30.20 mm,Bc 7.10 mm,Bh 8.80 mm,V1 23.00 mm,Vc 5.95 mm,and Bw/Vw 5.05 mm(P＜0.01,all).Conclusion The clinical performances of VBD is different,MS 3D-CTA is a very effective method for the diagnosis of VBD.

OBJECTIVE:To observe the effects as well as adverse durgs reactions of chemotherapy involving oxaliplatin in treatment of advanced nasopharyngeal carcinoma(NPC).METHODS:28patients with advanced NPC underwent chemotherapy with5-Fu,tetrahydrofolic acid calcium,and oxaliplatin for21days as one cycle,the therapeutic effects were evaluated after two cycles.Meanwhile the retrospective comparative study was made between the chemotherapy and the regular chemotherapy with5-Fu,tetrahydrofolic acid calcium,and platinum.RESULTS:Similar effects were observed in both regimens.How?ever,there were great differences in incidence ofⅢandⅣgrade nausea and vomiting,as well as renal function indices(P