INTRODUCTION: Lecanemab has shown promise in treating early Alzheimer's disease (AD), but its safety and efficacy in Chinese populations remain unexplored. This study aimed to evaluate the safety and 6-month clinical outcomes of lecanemab in Chinese patients with mild cognitive impairment (MCI) or mild AD. METHODS: In this single-arm, real-world study, participants with MCI due to AD or mild AD received biweekly intravenous lecanemab (10 mg/kg). The study was conducted at Hainan Branch, Ruijin Hospital Shanghai Jiao Tong University School of Medicine. Patient enrollment and baseline assessments commenced in November 2023. Safety assessments included monitoring for amyloid-related imaging abnormalities (ARIA) and other adverse events. Clinical and biomarker changes from baseline to 6 months were evaluated using cognitive scales (mini-mental state examination [MMSE], montreal cognitive assessment [MoCA], clinical dementia rating-sum of boxes [CDR-SB]), plasma biomarker analysis, and advanced neuroimaging. RESULTS: A total of 64 patients were enrolled in this ongoing real-world study. Safety analysis revealed predominantly mild adverse events, with infusion-related reactions (20.3%, 13/64) being the most common. Of these, 69.2% (9/13) occurred during the initial infusion and 84.6% (11/13) did not recur. ARIA-H (microhemorrhages/superficial siderosis) and ARIA-E (edema/effusion) were observed in 9.4% (6/64) and 3.1% (2/64) of participants, respectively, with only two symptomatic cases (one ARIA-E presenting with headache and one ARIA-H with visual disturbances). After 6 months of treatment, cognitive scores remained stable compared to baseline (MMSE: 22.33 ± 5.58 vs . 21.27 ± 4.30, P = 0.733; MoCA: 16.38 ± 6.67 vs . 15.90 ± 4.78, P = 0.785; CDR-SB: 2.30 ± 1.65 vs . 3.16 ± 1.72, P = 0.357), while significantly increasing plasma amyloid-β 42 (Aβ42) (+21.42%) and Aβ40 (+23.53%) levels compared to baseline. CONCLUSIONS: Lecanemab demonstrated a favorable safety profile in Chinese patients with early AD. Cognitive stability and biomarker changes over 6 months suggest potential efficacy, though high dropout rates and absence of a control group warrant cautious interpretation. These findings provide preliminary real-world evidence for lecanemab's use in China, supporting further investigation in larger controlled studies. REGISTRATION ClinicalTrials.gov , NCT07034222.
Humans ; Alzheimer Disease/drug therapy* ; Male ; Female ; Aged ; Middle Aged ; Cognitive Dysfunction/drug therapy* ; Aged, 80 and over ; Amyloid beta-Peptides/metabolism* ; Biomarkers ; East Asian People

AIM:This study aims to investigate distinct patterns in food reward behavior and neuroinflammato-ry responses within the ventral tegmental area(VTA)between obesity-prone(OP)and obesity-resistant(OR)rats,while elucidating their potential interplay.METHODS:Twenty-four male Sprague-Dawley rats(5-week-old)were adminis-tered a high-fat diet(HFD)for 8 weeks.Based on body weight tertiles,rats were stratified into OP(highest tertile,n=8)and OR(lowest tertile,n=8)groups.Food reward function was evaluated through conditioned place preference(CPP)test and operant food-seeking task(OFST).Serum lipid profiles were quantified via colorimetric microplate assays,with 24-hour energy expenditure monitored using CLAMS.Western blot and immunofluorescence assays quantified ionized cal-cium-binding adaptor molecule 1(Iba1)and tyrosine hydroxylase(TH)protein expression,while immunofluorescence lo-calized VTA-positive cell spatial distribution and density.RT-qPCR quantified mRNA expression of Iba1,TH,and proin-flammatory cytokines(TNF-α,IL-1β and IL-6).ELISA quantified proinflammatory cytokine protein concentrations.RE-SULTS:Following 8-week HFD exposure,OP rats exhibited elevated body weight,total food and calories,adiposity,Lee index,and levels of TG,LDL-C,TC,and NEFA,while HLD-C levels and 24-hour energy metabolism significantly decreased(P<0.05).OP rats demonstrated enhanced CPP preference for HFD-paired cues,elevated lever pressing fre-quency,and increased breakpoints versus OR counterparts(P<0.05),positively correlating with body weight(r=0.766,0.561 and 0.606;P<0.05).OP rats demonstrated elevated Iba1 positive cell density,protein and mRNA expression,and inflammatory mediators in VTA versus OR counterparts,contrasting with diminished TH positive neurons showing re-duced protein and mRNA levels(P<0.05).VTA neuroinflammatory mediators(Iba1,TNF-α,IL-1β and IL-6)exhibited inverse correlations with TH protein expression(r=-0.953,-0.866,-0.881 and-0.886;P<0.05).CONCLUSION:The OP rats exhibit attenuated reward sensitivity,elevated HFD preference,and increased palatable food-seeking behavior.These behavioral modifications correlate with VTA neuroinflammation suppressing dopaminergic(DA)biosynthesis.

AIM:This study aims to investigate distinct patterns in food reward behavior and neuroinflammato-ry responses within the ventral tegmental area(VTA)between obesity-prone(OP)and obesity-resistant(OR)rats,while elucidating their potential interplay.METHODS:Twenty-four male Sprague-Dawley rats(5-week-old)were adminis-tered a high-fat diet(HFD)for 8 weeks.Based on body weight tertiles,rats were stratified into OP(highest tertile,n=8)and OR(lowest tertile,n=8)groups.Food reward function was evaluated through conditioned place preference(CPP)test and operant food-seeking task(OFST).Serum lipid profiles were quantified via colorimetric microplate assays,with 24-hour energy expenditure monitored using CLAMS.Western blot and immunofluorescence assays quantified ionized cal-cium-binding adaptor molecule 1(Iba1)and tyrosine hydroxylase(TH)protein expression,while immunofluorescence lo-calized VTA-positive cell spatial distribution and density.RT-qPCR quantified mRNA expression of Iba1,TH,and proin-flammatory cytokines(TNF-α,IL-1β and IL-6).ELISA quantified proinflammatory cytokine protein concentrations.RE-SULTS:Following 8-week HFD exposure,OP rats exhibited elevated body weight,total food and calories,adiposity,Lee index,and levels of TG,LDL-C,TC,and NEFA,while HLD-C levels and 24-hour energy metabolism significantly decreased(P<0.05).OP rats demonstrated enhanced CPP preference for HFD-paired cues,elevated lever pressing fre-quency,and increased breakpoints versus OR counterparts(P<0.05),positively correlating with body weight(r=0.766,0.561 and 0.606;P<0.05).OP rats demonstrated elevated Iba1 positive cell density,protein and mRNA expression,and inflammatory mediators in VTA versus OR counterparts,contrasting with diminished TH positive neurons showing re-duced protein and mRNA levels(P<0.05).VTA neuroinflammatory mediators(Iba1,TNF-α,IL-1β and IL-6)exhibited inverse correlations with TH protein expression(r=-0.953,-0.866,-0.881 and-0.886;P<0.05).CONCLUSION:The OP rats exhibit attenuated reward sensitivity,elevated HFD preference,and increased palatable food-seeking behavior.These behavioral modifications correlate with VTA neuroinflammation suppressing dopaminergic(DA)biosynthesis.

Objective We aimed to establish and evaluate a rat model of post-infectious irritable bowel syndrome(PI-IBS)with the pattern of liver depression and spleen deficiency coupled with dampness.Methods First,200 rats were randomly divided into the normal group,the infection group,the infection+stress group,the infection+stress+external dampness group,and the acetic acid+stress group(n=40 rats per group)for eight weeks.The rats were treated with Trichinella spiralis infection,chronic restraint stress,an artificial high-humidity climate,and/or acetic acid enema.Weight growth rate,24-hour food intake and water intake,and the fecal moisture percentage were recorded.The open field test and the sucrose consumption test were used to determine the behavioral characteristics of rats in each group.The abdominal withdrawal reflex(AWR)test was used to determine visceral sensitivity.The contents of 5-hydroxytryptamine(5-HT)and aquaporin 4(AQP4)in colon tissue were detected by ELISA.Results Compared with the normal group,the weight growth rate of rats in the infection+stress+external dampness group and the acetic acid+stress group was lower from Week 1 to Week 8 of modeling.The 24-hour food intake of rats in the infection+stress+external dampness group and the infection+stress group was lower than that in the normal group from Week 2 to Week 8 of modeling.At the end of week 2 of modeling,the 24-hour water intake of rats in the infection+stress+external dampness group,the acetic acid+stress group,and the infection+stress group was lower than that in the normal group.The fecal moisture percentage of rats in the infection+stress+external dampness group was higher than that in the normal group at the end of Week 1,6,and 8(P＜0.05).At the end of Week 4 of modeling,the total distance in the open field test in the infection+stress+external dampness group and the acetic acid+stress group was shorter than that in the normal group.The sugar preference rate in the infection+stress+external dampness group was lower than that in the normal group at the end of 1,4,and 8 weeks and lower than that in the acetic acid+stress group at the end of Week 4 and 8(P＜0.05).The AWR scores of rats in the infection+stress+external dampness group were higher than those in the normal group after week 1 at 60 and 80 mmHg(1 mmHg≈0.133 kPa),after Week 4 at 40,60,and 80 mmHg,and after Week 6 and 8 at 20,40,and 80 mmHg(P＜0.05).At the end of Week 2 and 4,a large number of inflammatory cells were infiltrated in the colonic mucosa of the intervention groups,and the inflammation score was higher than that of the normal group(P＜0.05).At the end of weeks 6 and 8,the inflammatory cell infiltration in the intestinal mucosa of the intervention groups was not obvious,and the colonic mucosa returned to normal.At the end of weeks 6 and 8,the 5-HT content was higher in the infection+stress group,the infection+stress+external dampness group,and the acetic acid+stress group than in the normal group(P＜0.05).After Week 4,the AQP4 content was lower in the infection+stress+external dampness group and the acetic acid+stress group than that in the normal group(P＜0.05).After week 6,compared to the normal group,the AQP4 content was lower in all groups except for the acetic acid+stress group,and the AQP4 content in the infection+stress+external dampness group was lower than that in the acetic acid+stress group.After week 8,only in the infection+stress+external dampness group the AQP4 content was lower than in the normal group(P＜0.05).Conclusion The combination of Trichinella spiralis infection,chronic restraint stress,and an artificial high-humidity climate can be used to prepare a relatively stable and reliable rat model of PI-IBS with the pattern of liver depression and spleen deficiency with dampness.

Objective:To study the clinical efficacy of botulinum toxin type A injection in the treatment of upper facial dynamic wrinkles.Methods:A total of 35 patients with upper facial dynamic wrinkles treated in our hospital from February 2016 to October 2016 were selected as research objects.All the patients were treated with botulinum toxin type A.The excellence rate of 30min after treatment,3 d after treatment and 7 d after treatment were evaluated and compared.The facial wrinkles severity index of the patients were evaluated by the facial wrinkles scale (FWS),and the differences of the FWS index before treatment,4 weeks after treatment and 6 months after treatment were compared.The adverse reactions,like upper facial tension,fatigue and local injection of ecchymosis after treatment were observed and compared.Results:The excellence rate of the patients after treatment of 30 min,3 d and 7 d were gradually increased.The excellence rate of the patients after treatment of 3 d and 7 d were significantly higher than that of after treatment of 30 min,and the excellence rate of the patients after treatment of 7 d were significantly higher than that of after treatment of 3 d (P＜0.05).The FWS index of the patients after treatment of 4 weeks and 6 months were significantly lower than those of before treatment,and the FWS index of the patients after treatment of 4 weeks were significantly lower than that of 6 months after treatment(P＜0.05).After treatment,only 4 patients had slight discomfort and all of these symptoms were disappeared after 3-4 days,there was no effect on their normal work and life.Conclusion:Botulinum toxin type A injection has a remarkable clinical curative effect in the treatment of patients with upper facial dynamic wrinkles,can maintain tor a long time,and also has good safety,it is worthy clinical application.

Objective:To investigate the curative effect of botulinum toxin type A combined with plastic operation of pouches on patients with eye skin relaxation and its impact on patients' satisfaction,and to provide reference for clinical application.Methods:A total of 60 patients with eye skin relaxation,who were treated in Baoji Central Hospital from June 2015 to June 2016,were selected as subjects and divided into control group (n=28) and treatment group (n=32) according to the patients' choices.The patients of control group were treated with plastic operation of pouches,while the patients of treatment group were treated with botulinum toxin type A combined with plastic operation of pouches.The two groups were followed up for 6 months.The improvement of crow's feet,the relief of pouches and the improvement of eye skin gloss of the patients after operation were observed and recorded.The incidence of complications were also observed.At the end of the follow-up,the satisfaction degrees of the patients were investigated with the self-made questionnaire.Results:After treatment,the improvement rate(100％) of crow's feet of the treatment group was significantly higher than that(60.71％) of the control group;the improvement rate (96.88％) of pouches of the treatment group was significantly higher than that (32.14％) of the control group;the improvement rate (87.50％) of eye skin gloss of the treatment group was significantly higher than that (53.57％) of the control group,and the differences were statistically significant (all P＜0.05).After treatment,there was no significant difference in the incidence of incision swelling,small amount of secretions and small hematoma between the two groups (P＞0.05),the operation eye swelling and incision bruises outer canthus in the control group were more than those in the treatment group (P＜0.05).The total satisfaction rate [93.75％ (30/32)] of the treatment group was significantly higher than that[67.86％ (19/28)] of the control group,the difference was statistically significant (P＜0.05).Conclusion:Botulinum toxin type A combined with plastic operation of pouches in the treatment of eye skin relaxation has a better efficacy and safety,which can significantly reduce the incidence of operation eye swelling and incision bruises outer canthus,and improve patients' satisfaction rate.It is worthy of clinical application.

Objective To evaluate the value of DWI and ADC value in monitoring the chemotherapy response of advanced gastric carcinoma dynamically.Methods 42 advanced gastric carcinoma patients who were confirmed by histopathology underwent T2 WI and DWI examinations at pre-chemotherapy,post-chemotherapy 3 d,7 d,30 d and 60 d respectively.The longest diameters of tumor pre-chemotherapy and post-chemotherapy 60 d were measured on axial T2 WI,meanwhile the ADC values at different time points were calculated.The mean ADC value among pre-and post-chemotherapy of each group (PR and SD)was compared.Results The ADC value of PR group increased gradually.The mean ADC value before therapy was statistically lower than those at differ-ent time points post-chemotherapy (P < 0.05).The ADC value of SD group increased gradually from pre-chemotherapy to post-chemotherapy 30 d,and then the ADC value decreased at post-chemotherapy 60 d.The differences of the mean ADC values in differ-ent time points were statistically significant (P < 0.05).Conclusion DWI and ADC value can dynamically,quantitatively and early detect and monitor the chemotherapy response of advanced gastric carcinoma.

The article involved in the morphology and the growth of Monascus on surface fermentation The colony area of former period, and phenetic volume the distribution of growth on the basis of color of latter period reflect the mycelia activity The kinetics model of morphological varies was established, which agree with the normal kinetics model