This study aims to investigate the medication rules of patented traditional Chinese medi-cine(TCM)compound formulas and molecular mechanisms of core drugs for treating sepsis using data mining and network pharmacology approaches.In the present study,we first searched the PubMed database,Web of Science database,and the China National Knowledge Infrastructure(CNKI)since the establishment of the library to April 30,2024 for the relevant literature on the treatment of sepsis by traditional Chinese medicine.The prescriptions were then statistically ana-lyzed for drug frequency and association analysis to obtain the core drugs.Then we screened the ef-fective active ingredients of the core drugs by TCMSP and other database platforms,obtained sep-sis-related genes in GeneCards and other databases,and statistically intersected targets,and predic-ted the mechanism of action of the core TCMs by subjecting the intersected targets to PPI analy-sis,GO function and KEGG pathway enrichment analysis.Finally,the relationship between key tar-gets and herbal components was examined in reverse by molecular docking method.The results showed that 64 compound formulas were obtained,with a total of 150 Chinese medicines,which were mostly sweet in taste,cold in nature,and belonged to the spleen,stomach and intestinal me-ridians.According to the association rules,the core drugs were identified as"mirabilite-peach ker-nel-rheum officinale".There were 79 intersecting targets between the core drugs and sepsis,with core targets such as IL-1β,EGFR and SRC.MAPK,TNF,IL-17 and other signaling pathways are involved to mediate inflammatory responses,apoptosis and other biological processes to exert ther-apeutic effects on sepsis.The molecular docking results indicated that the docking activity of the key targets with the main components of the drug,and sennoside E_qt has the lowest binding ener-gy and the best docking activity with SRC.In conclusion,this study showed that the prescription of Chinese medicine for sepsis is mostly based on tonifying the spleen and clearing heat.The mecha-nism of action of the core drug"mirabilite-peach kernel-rheum officinale"in the treatment of sep-sis is multilevel and multifaceted,which provides a certain theoretical basis for the treatment of sepsis by traditional Chinese medicine.

This study aims to investigate the medication rules of patented traditional Chinese medi-cine(TCM)compound formulas and molecular mechanisms of core drugs for treating sepsis using data mining and network pharmacology approaches.In the present study,we first searched the PubMed database,Web of Science database,and the China National Knowledge Infrastructure(CNKI)since the establishment of the library to April 30,2024 for the relevant literature on the treatment of sepsis by traditional Chinese medicine.The prescriptions were then statistically ana-lyzed for drug frequency and association analysis to obtain the core drugs.Then we screened the ef-fective active ingredients of the core drugs by TCMSP and other database platforms,obtained sep-sis-related genes in GeneCards and other databases,and statistically intersected targets,and predic-ted the mechanism of action of the core TCMs by subjecting the intersected targets to PPI analy-sis,GO function and KEGG pathway enrichment analysis.Finally,the relationship between key tar-gets and herbal components was examined in reverse by molecular docking method.The results showed that 64 compound formulas were obtained,with a total of 150 Chinese medicines,which were mostly sweet in taste,cold in nature,and belonged to the spleen,stomach and intestinal me-ridians.According to the association rules,the core drugs were identified as"mirabilite-peach ker-nel-rheum officinale".There were 79 intersecting targets between the core drugs and sepsis,with core targets such as IL-1β,EGFR and SRC.MAPK,TNF,IL-17 and other signaling pathways are involved to mediate inflammatory responses,apoptosis and other biological processes to exert ther-apeutic effects on sepsis.The molecular docking results indicated that the docking activity of the key targets with the main components of the drug,and sennoside E_qt has the lowest binding ener-gy and the best docking activity with SRC.In conclusion,this study showed that the prescription of Chinese medicine for sepsis is mostly based on tonifying the spleen and clearing heat.The mecha-nism of action of the core drug"mirabilite-peach kernel-rheum officinale"in the treatment of sep-sis is multilevel and multifaceted,which provides a certain theoretical basis for the treatment of sepsis by traditional Chinese medicine.

Objective To evaluate the efficacy of minimally invasive neuroendoscopy combined with intraventricular drainage in the treatment of intraventricular hemorrhage.Methods A retrospective analysis was conducted on the clinical data of 88 patients with intraventricular hemorrhage who underwent surgical treatment from January 2018 to December 2023.All patients received bilateral ventricular indwelling external drainage tubes.Among them,37 cases were included in the endoscopy group.The bleeding side or side with more blood accumulation underwent a midline fistula.With the assistance of neuroendoscopy,the intraventricular blood clots were removed and a ventricular drainage tube was inserted.The contralateral ventricle was punctured and a ventricular drainage tube was inserted;The remaining 51 cases were included in the drainage group,in which bilateral ventricular drainage tubes were placed according to the traditional intraventricular drainage protocol.Results There was no significant statistical difference(P>0.05)between the endoscopic group and the drainage group in terms of mortality rate(10.8%vs.13.7%),rebleeding rate(8.1%vs.11.8%,P=0.576),intracranial infection rate(10.8%vs.17.6%,P=0.372),and hydrocephalus rate(10.8%vs.23.5%,P=0.166).Similarly,no significant statistical difference was found in the total hospitalization cost between the endoscopy group and the drainage group[(90700±73000)yuan vs.(99500±66000)yuan]and the GOS score during the 6-month follow-up(3.16±1.14 vs 3.02±1.12)showed(P>0.05).However,the postoperative GCS score improvement was significantly better in the endoscopic group than in the drainage group[2.00(2.00,4.00)vs.1.00(0.00,3.00),P=0.002].The duration of the extracranial drainage catheter placement was significantly shorter in the endoscopic group than in the drainage group[(7.16±2.99)days vs.(10.12±3.93)days,P<0.001].Conclusion Neuroendoscopy-assisted evacuation of intraventricular hemorrhage facilitates effective hematoma clearance,reduces the duration of ventricular drainage catheterization,and may contribute to improved patient outcomes.

Objective To evaluate the efficacy of minimally invasive neuroendoscopy combined with intraventricular drainage in the treatment of intraventricular hemorrhage.Methods A retrospective analysis was conducted on the clinical data of 88 patients with intraventricular hemorrhage who underwent surgical treatment from January 2018 to December 2023.All patients received bilateral ventricular indwelling external drainage tubes.Among them,37 cases were included in the endoscopy group.The bleeding side or side with more blood accumulation underwent a midline fistula.With the assistance of neuroendoscopy,the intraventricular blood clots were removed and a ventricular drainage tube was inserted.The contralateral ventricle was punctured and a ventricular drainage tube was inserted;The remaining 51 cases were included in the drainage group,in which bilateral ventricular drainage tubes were placed according to the traditional intraventricular drainage protocol.Results There was no significant statistical difference(P>0.05)between the endoscopic group and the drainage group in terms of mortality rate(10.8%vs.13.7%),rebleeding rate(8.1%vs.11.8%,P=0.576),intracranial infection rate(10.8%vs.17.6%,P=0.372),and hydrocephalus rate(10.8%vs.23.5%,P=0.166).Similarly,no significant statistical difference was found in the total hospitalization cost between the endoscopy group and the drainage group[(90700±73000)yuan vs.(99500±66000)yuan]and the GOS score during the 6-month follow-up(3.16±1.14 vs 3.02±1.12)showed(P>0.05).However,the postoperative GCS score improvement was significantly better in the endoscopic group than in the drainage group[2.00(2.00,4.00)vs.1.00(0.00,3.00),P=0.002].The duration of the extracranial drainage catheter placement was significantly shorter in the endoscopic group than in the drainage group[(7.16±2.99)days vs.(10.12±3.93)days,P<0.001].Conclusion Neuroendoscopy-assisted evacuation of intraventricular hemorrhage facilitates effective hematoma clearance,reduces the duration of ventricular drainage catheterization,and may contribute to improved patient outcomes.

Objective: To analyze the epidemiological characteristics and trends of other infectious diarrhea among children under 18 years old in Guangzhou City from 2014 to 2020, and to explore the correlation between climatic factors and the incidence of the disease, so as to provide reference for the early prevention of infectious diseases. Methods: The data of cases of other infectious diarrhea and meteorological data of children under 18 years old in Guangzhou City from 2014 to 2020 were collected through the Chinese Infectious Disease Reporting System and the Guangzhou Meteorological Bureau. The correlation between meteorological factors and the incidence of other infectious diarrhea was analyzed using negative binomial regression. Results: A total of 104 566 cases of other infectious diarrhea among children under 18 years old were reported in Guangzhou City from 2014 to 2020, with a male to female ratio of 1.48∶1. The incidence rate was the highest in 2017 (980.83 per 100 000) and the lowest in 2020 (388.22 per 100 000). The peak of incidence occurred from October to March of the following year. Children under 5 years old accounted for 87.95% of all cases. The number of cases of other infectious diarrhea was negatively correlated with the temperature of the previous 6 days ( IRR = -0.07 ), and positively correlated with the temperature difference on the day of onset ( IRR =0.02) (both P <0.05). It was also positively correlated with the wind speed of the previous 7 days ( IRR=0.07, P <0.05), but there was no statistically significant correlation with the relative humidity on the day of onset ( IRR=-0.00, P >0.05). Conclusions Low temperature, large temperature difference, and high wind speed can increase the risk of other infectious diarrhea. It is necessary to strengthen the prediction and early warning in conjunction with meteorological changes, and warn kindergartens and schools to enhance preventive measures against the clustering of other infectious diarrhea cases.

Turning to critical illness is a common stage of various diseases and injuries before death. Patients usually have complex health conditions, while the treatment process involves a wide range of content, along with high requirements for doctor′s professionalism and multi-specialty teamwork, as well as a great demand for time-sensitive treatments. However, this is not matched with critical care professionals and the current state of medical care in China. Telemedicine, which shortens the distance of medical professionals and the gap of disease diagnosis and treatments in various regions through electronic information, can effectively solve the current problem. Therefore, there is an urgent need to develop a standardized, high-quality visualization telemedicine round system .Therefore, experts have been organized to search domestic and foreign literature on telemedicine round for critically ill patients and to form this consensus based on clinical experiences so as to further improve the level of critical care treatments in regions.

Pneumothorax during pediatric laparoscopic surgery is a potentially fatal complication that may not be promptly recognized. It can occur due to congenital anatomical abnormalities, pre-existing pulmonary disease, or operative factors during laparoscopy. Clinical presentation may range from asymptomatic to acute respiratory distress, pleuritic chest pain, and even life-threatening circulatory collapse. Here, we report a case of sudden intraoperative pneumothorax accompanied by extensive subcutaneous emphysema of the neck and chest wall during laparoscopic high ligation of the hernial sac in a child. The child presented with a reducible left lower abdominal mass and mild pain 3 days prior but did not seek medical attention. Symptoms worsened 1 day prior to admission, with difficulty reducing the mass. On April 8, 2021, the patient was admitted to the Department of Anesthesiology, Zhuzhou Hospital Affiliated to Xiangya School of Medicine of Central South University, with a diagnosis of "left inguinal hernia." On the second day of hospitalization, laparoscopic high ligation of the left inguinal hernia sac was performed under general anesthesia. During the procedure, the patient developed a sudden increase in airway pressure, marked hemodynamic fluctuations, crepitus in the neck and right anterior chest regions, and significantly diminished breath sounds in the right lung. Emergent bedside chest X-ray confirmed a right-sided pneumothorax. Immediate intervention including thoracic needle decompression, closed thoracic drainage, the lung re-expansion was performed. The patient was discharged on the 7th postoperative day with full recovery. This case highlights the need for clinicians to remain vigilant for iatrogenic pneumothorax during pediatric laparoscopic surgery. Close intraoperative monitoring of vital signs is crucial for early detection, recognition, and timely management of pneumothorax to ensure patient safety during minimally invasive procedures.
Humans ; Laparoscopy/methods* ; Pneumothorax/etiology* ; Ligation/methods* ; Hernia, Inguinal/surgery* ; Male ; Intraoperative Complications/etiology* ; Child ; Herniorrhaphy/methods* ; Female ; Subcutaneous Emphysema/etiology*

Traditional Chinese medicine formula (TCMF) represents a fundamental component of Chinese medical practice, incorporating medical knowledge and practices from both Han Chinese and various ethnic minorities, while providing comprehensive insights into health and disease. The foundation of TCMF lies in its holistic approach, manifested through herbal compatibility theory, which has emerged from extensive clinical experience and evolved into a highly refined knowledge system. Within this framework, Chinese herbal medicines exhibit intricated characteristics, including multi-component interactions, diverse target sites, and varied biological pathways. These complexities pose significant challenges for understanding their molecular mechanisms. Contemporary advances in artificial intelligence (AI) are reshaping research in traditional Chinese medicine (TCM), offering immense potential to transform our understanding of the molecular mechanisms underlying TCMFs. This review explores the application of AI in uncovering these mechanisms, highlighting its role in compound absorption, distribution, metabolism, and excretion (ADME) prediction, molecular target identification, compound and target synergy recognition, pharmacological mechanisms exploration, and herbal formula optimization. Furthermore, the review discusses the challenges and opportunities in AI-assisted research on TCMF molecular mechanisms, promoting the modernization and globalization of TCM.
Artificial Intelligence ; Drugs, Chinese Herbal/pharmacokinetics* ; Humans ; Medicine, Chinese Traditional ; Animals

Objective : To investigate the role and mechanism of long non-coding RNA(lncRNA) H19 in osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs) and bone regeneration in mice with foot fracture. Methods: BMSCs were divided into microrNA-149-5 p(miR-149-5 p)-mimic negative control(miR-mimicNC) group and miR-149-5p-mimic(miR-mimic) group and transfected with IncRNA H19-wt and IncRNA H19,respectively.Mut recombinant plasmid,or bone morphogenetic protein 2(BMP2) 3'UTR-wt and BMP2 3' UTRmut recombinant plasmid were transfected,respectively.Dual-luciferase reporter gene assay was used to detect the luciferase activity of each group.To study the regulatory effect and mechanism of IncRNA H19 on osteogenic differentiation in vitro,BMSCs were divided into control group and osteogenic induction group(osteogenic group).After osteogenic induction,BMSCs were transfected with corresponding plasmids and divided into osteogenic+si-NC group,osteogenic+si-H19 group,osteogenic+si-H19+miR-inhibitor-NC group,osteogenic+si-H19+miR-inhibitor group,osteogenic+si-H19+pcDNA-NC group,and osteogenic group si-H19+pcDNA-BMP2 group.Osteogenic differentiation was evaluated by alkaline phosphatase(ALP) activity assay and alizarin red staining.A mouse foot fracture model was established,and 36 mice were randomly divided into sham operation group,model group,model+pcD-null group,and model+pcD-H19 group,with 9 mice in each group.Osteogenic differentiation was assessed by ALP activity assay.Real-time fluorescent quantitative PCR(qPCR) was used to detect the expression of IncRNA H19,miR-149-5p,and BMP2.Western blot was used to detect the expression of BMP2,OCN,OSX,RUNX2,and OPN. Results : In cells transfected with lncRNA H19-wt , the luciferase activity in miR-mimic group was lower than that in miR-mimic-NC group (P < 0. 05) . The luciferase activity of miR-mimic group was lower than that of miR-mimic-NC group ( P < 0. 05) . Compared with the control group , the alkaline phosphatase activity , the degree of cell mineralization and the expression of lncRNA H19 , BMP2 , OCN , OSX , RUNX2 and OPN increased , and the expression of miR-149-5p decreased in the osteogenic group ( P < 0. 05) . Compared with the osteogenesis + si-NC group , the alkaline phosphatase activity , the degree of cell mineralization and the expression of lncRNA H19 , BMP2 , OCN , OSX , RUNX2 and OPN significantly decreased , and the ex- pression of miR-149-5p increased in the osteogenesis + si-H19 group (P < 0. 05) . Compared with the osteogenesis + si-H19 + miR-inhibitor-NC group , the ALP activity , the degree of cell mineralization and the expression of ln- cRNA H19 , BMP2 , OCN , OSX , RUNX2 and OPN increased in the osteogenesis + si-H19 + miR-inhibitor group . The expression of miR-149-5p significantly decreased (P < 0. 05) . Compared with the osteogenic + si-H19 + pcD- NA-NC group , the alkaline phosphatase activity , the degree of cell mineralization and the expression of BMP2 , OCN , OSX , RUNX2 and OPN significantly increased in the osteogenic + si-H19 + pcDNA-BMP2 group ( P < 0. 05) . Compared with the sham-operation group and the model group , the alkaline phosphatase activity and the expression of lncRNA H19 , BMP2 , OCN , OSX , RUNX2 and OPN decreased , and the expression of miR-149-5p increased (P < 0. 05) . Compared with the model + pcD-null group , the alkaline phosphatase activity and the ex- pression of lncRNA H19 , BMP2 , OCN , OSX , RUNX2 and OPN significantly increased , and the expression of miR-149-5p decreased in the model + pcD-H19 group (P < 0. 05) . Conclusion lncRNA H19 promotes osteogenic differentiation of BMSC s and bone regeneration in mice with foot fracture through miR-149-5p/BMP2 axis .

Objective To observe the effects of Xuanfei Tongfu formula on the prognosis of patients with severe pneumonia.Methods Sixty patients with severe pneumonia admitted to the First People's Hospital of Fuyang District,Hangzhou City from June 1,2022 to April 1,2024 were selected as the research subjects.Patients were divided into control group(31 cases)and observation group(29 cases)by randomly number table method.Upon hospital admission,the control group received conventional Western medicine treatment,while the observation group received Xuanfei Tongfu formula in addition to conventional Western medicine treatment[Rhubarb 8 g(decocted later),Aurantii Fructus Immaturus 12 g,Magnoliae Officinalis Cortex 12 g,Agastache Rugosa 10 g,Tatarian Aster Root 15 g,Natrii Sulfas Exsiccatus 10 g(dissolved in water),decocted to 150 mL each time and taken],once a day for 5 days.Observe the changes of inflammatory indicators such as serum levels of C-reactive protein(CRP),procalcitonin(PCT),interleukin-6(IL-6)in both groups before and after treatment.To evaluate the severity indicators of diseases such as acute physiology and chronic health evaluationⅡ(APACHEⅡ)and sequential organ failure assessment(SOFA),as well as the clinical efficacy after treatment.Record the duration of mechanical ventilation,intensive care unit(ICU)length of stay and total hospitalization length of the two groups,calculate the oxygenation index level,and statistically analyze the 28-day survival rate.Results Compared with before treatment,the levels of inflammatory indicators CRP,PCT,and IL-6,as well as the scores of APACHEⅡand SOFA in both groups were significantly lower than those after treatment,the oxygenation index was significantly higher than that before treatment,and the changes in the observation group were more obvious than those in the control group[CRP(mg/L):28±17 vs.50±34,PCT(μg/L):2.0±1.5 vs.4.1±2.7,IL-6(ng/L):52±28 vs.97±61,APACHEⅡscore:12.8±5.1 vs.16.1±6.1,SOFA score:3.9±1.8 vs.6.2±2.7,oxygenation index(mmHg,1 mmHg≈0.133 kPa):302±57 vs.252±85,all P<0.05].Compared with the control group,the observation group exhibited a markedly reduced duration of mechanical ventilation(days:8.3±3.8 vs.11.4±6.4,P<0.05),and a significantly higher total effective rate of clinical efficacy[82.76%(24/29)vs.54.84%(17/31),P<0.05].Conclusion The application of Xuanfei Tongfu formula can effectively enhance the clinical treatment outcomes for severe pneumonia,reduce systemic inflammatory responses,promote organ function recovery,and improve the prognosis of patients.