Seven novel acylphloroglucinol-sesquiterpenoid adducts, designated as dryatraols J-P (1-7), were isolated from the rhizomes of Dryopteris atrata (Wall. ex Kunze) Ching. The structures, including absolute configurations, were elucidated using comprehensive spectroscopic data, calculated ¹³C Nuclear Magnetic Resonance-Diastereotopic Probability Assignment Plus (¹³C NMR-DP4+) probability analysis, and ECD calculations. These structures represent a rare subclass of carbon skeleton of acylphloroglucinol-sesquiterpenoid adducts with a furan ring connecting the acylphloroglucinol and sesquiterpenoid moieties. Notably, compounds 1-6 are the first reported examples of acylphloroglucinol-sesquiterpenoid adducts with dimeric acylphloroglucinol incorporated into the aristolane- or rulepidanol-type sesquiterpene, while compound 7 features a hydroxylated monomeric acylphloroglucinol motif. A preliminary evaluation of their antiviral activities revealed that compounds 1-6 exhibited more potent activities against respiratory syncytial virus (RSV) with IC₅₀ values ranging from 0.75 to 3.12 μmol·L^-1 compared to the positive control (ribavirin).
Antiviral Agents/isolation & purification* ; Phloroglucinol/isolation & purification* ; Sesquiterpenes/isolation & purification* ; Molecular Structure ; Dryopteris/chemistry* ; Respiratory Syncytial Viruses/drug effects* ; Humans ; Rhizome/chemistry* ; Drugs, Chinese Herbal/pharmacology*

Polyhydroxyalkanoates are polyesters of hydroxyalkanoates synthesized by many bacteria and haloarchaea as carbon and energy storage materials. There are more than 150 types of polyhydroxyalkanoate monomers reported, resulting in a variety of Polyhydroxyalkanoates with diverse properties. The material variability, nonlinear optical properties, piezoelectric properties, gas barrier properties, thermoplasticity, biodegradability, and biocompatibility allow polyhydroxyalkanoates to be used for plastic packaging, chiral chemicals generation, medicine, agriculture and bio-energy fields. This review introduces the current applications and future development of polyhydroxyalkanoates.

Objective To evaluate the clinical efficacy of polyethylene glycols (PEG) on functional constipation in children by Meta-analysis.Methods The Medline,Pubmed,Cochrane database,Embase,VIP database,China Knowledge Resource Integrated Database (CNKI) and WANFANG MED ONLINE resources were reviewed for the treatment with polyethylene glycols on functional constipation in children from the inception to December 2016 to collect all randomized controlled trials (RCTs) or comparative prospective studies.Revman 5.3 software was used to assess risk of bias of the Cochrane Collaboration's tool.A Meta analysis of the therapeutic effect of polyethylene glycols on functional constipation in children was conducted by Stata1 1.0 software.The pooled standardized mean difference (SMD) and 95％ confidence interval (95％ C/) were calculated in order to know the clinical efficacy of polyethylene glycols on functional constipation in children.Results Seven literatures were included in our Meta analysis.There were 662 children were included in this study,and they were divided into PEG treatment group (n=318) and non-PEG treatment group (n=344).The forest plot results of Meta analysis showed that the main effect index weekly stool frequency was significantly higher in PEG treatment group than that of lactulose group,according to the different control measures.The main effect index weekly stool frequency was significantly higher in PEG treatment group than that of non-PEG treatment group,according to the mapping forest chart displayed different clinical effect observation time points.Results from Meta analysis showed that the secondary effect index successful stool consistency improvement was significantly higher in PEG treatment group than that of non-PEG treatment group,according to different observation time points.Conclusion Polyethylene glycols is safe and effective in the treatment of functional constipation in children.

OBJECTIVETo investigate the significance of monitoring procalcitonin (PCT) when applying antibiotics to trichlorethylene (TCE)-induced dermatitis.

METHODSOne hundred and two patients who were hospitalized and recovered from TCE-induced dermatitis in our hospital from 2006 to 2013 were enrolled as subjects. Based on whether the PCT level was monitored or not, we divided patients into regular group and PCT group. For the regular group, we applied antibiotic treatment and determined the course of treatment based on clinical symptoms, laboratory test results, medical imaging results, and bacterial culture. For the PCT group, in addition to the above treatments, antibiotic treatment was applied when the PCT level was not lower than 0.25 ng/ml and stopped when the PCT level was lower than 0.25 ng/ml. The distribution of bacterial infection sites, type of bacteria, type of antibiotics, average period of hospitalization, and course of antibiotic treatment were compared between the two groups.

RESULTSThere were no significant differences in the distribution of bacterial infection sites, type of bacteria, type of antibiotics, and average period of hospitalization between the two groups (P > 0.05). The course of antibiotic treatment for the PCT group was significantly shorter than that for the regular group (25.37 ± 11.66 vs 20.58 ± 7.53 d, P < 0.05).

CONCLUSIONUnder similar conditions of bacterial infection, antibiotic treatment of TCE-induced dermatitis based on the serum PCT level can significantly shorten the course of treatment and avoid the abuse of antibiotics.

Anti-Bacterial Agents ; therapeutic use ; Bacteria ; Bacterial Infections ; Calcitonin ; analysis ; Calcitonin Gene-Related Peptide ; Drug Eruptions ; drug therapy ; Hospitalization ; Humans ; Monitoring, Physiologic ; Protein Precursors ; analysis ; Trichloroethylene ; toxicity

The achenes morphological and micro-morphological characteristics of six species of genus Taraxacum from northeastern China as well as SRAP cluster analysis were observed for their classification evidences. The achenes were observed by microscope and EPMA. Cluster analysis was given on the basis of the size, shape, cone proportion, color and surface sculpture of achenes. The Taraxacum inter-species achene shape characteristic difference is obvious, particularly spinulose distribution and size, achene color and achene size; with the Taraxacum plant achene shape the cluster method T. antungense Kitag. and the T. urbanum Kitag. should combine for the identical kind; the achene morphology cluster analysis and the SRAP tagged molecule systematics's cluster result retrieves in the table with "the Chinese flora". The class group to divide the result is consistent. Taraxacum plant achene shape characteristic stable conservative, may carry on the inter-species division and the sibship analysis according to the achene shape characteristic combination difference; the achene morphology cluster analysis as well as the SRAP tagged molecule systematics confirmation support dandelion classification result of "the Chinese flora".
Base Sequence ; China ; Cluster Analysis ; DNA, Plant ; genetics ; Fruit ; anatomy & histology ; ultrastructure ; Genetic Markers ; genetics ; Genetic Variation ; Genotype ; Phylogeny ; Plants, Medicinal ; anatomy & histology ; genetics ; Polymorphism, Genetic ; Species Specificity ; Taraxacum ; anatomy & histology ; classification ; genetics

OBJECTIVETo determine cytology chromosome numbers, doubling, type, karyotype formulae of seven kinds of Taraxacum.

METHODSeven kinds of dandelion in Northeast China were karyomorphologically studied by conventional pressed slice method.

RESULTThe interphase nuclei and prophase chromosomes of all species were found to be of the complex chromocenter type and the interstitial type respectively. The somatic chromosomes showed polysomaty within or among species. The range of chromosome numbers was from 16 to 32, and 24 was preponderant. It is firstly confirmed that the chromosome numbers of T. ohwianum were 2n = 2x = 16 of and those of T. variegatum 2n = 4x = 32 of, and those of other five kinds of dandelion 2n = 3x = 24. Karyotype was diverse and consisted of metacentric, submetacentric and satellites. The relative lengh of chromosome varies from 3.74 to 27.68, asymmetry index was between 59.68% and 64.02%; The karyotype type was belonged to "1A", "2A"and "2B" extensively, to ensure the T. ohwianum and T. variegatum evolutional karyotype type and genetic inheritance pattern.

CONCLUSIONAccording to results of the cytology karyotype we suggest that T. antungense and T. urbanum could be merged, the result was consistent with Flora Reipublicae Popularis Sinicae (FRPS). This paper reports systematically cytology karyotype feature of seven kinds of dandelions in Northeast, provides the cytology theoretical basis for further development and use of the resource and genetic breeding research of dandelions.

China ; Chromosomes, Plant ; genetics ; Karyotype ; Plants, Medicinal ; classification ; cytology ; genetics ; Taraxacum ; classification ; cytology ; genetics

OBJECTIVETo study the sympathetic skin response (SSR) to the effects of N-hexane on autonomic nerves function in patients with chronic N-hexane poisoning.

METHODSThe subjects in present study included 30 controls and 37 cases with chronic N-hexane poisoning. Also 37 patients were divided into 3 subgroups (mild, moderate and severe poisoning) according to diagnostic criteria of occupational diseases. All subjects were examined by SSR test and nerve conduction velocity (NCV) test. All patients were reexamined by SSR and NCV every 1 ∼ 2 months. The differences in SSR parameters (latency, amplitude) among groups were observed. In the severe poisoning subgroup, the changes of SSR and NCV parameters (conduction velocity, amplitude) in different poisoning stages were observed.

RESULTSThere were significant differences in SSR latency of upper extremity among groups and the significant differences in SSR amplitude of upper and lower extremity among groups (P < 0.05). No significant differences in SSR parameters were found between the adjacent groups (P > 0.05). There were significant differences in SSR latency of upper extremity during different periods and the significant differences in SSR amplitude of upper and lower extremity during different periods among all groups (P < 0.05). The change of SSR parameters consistent with that in NCV. The longest SSR latency of upper extremity and the smallest SSR amplitudes of upper and lower extremity appears 1 - 2 months earlier than that of the smallest action potential amplitude.

CONCLUSIONThe damage of autonomic nerves induced by N-hexane increased with poisoning progresses. The damage of autonomic nerves corresponded with the damage of myelin sheath of large myelinated nerves, but which appeared 1 - 2 months earlier than the damage of axon of large myelinated nerves. SSR test may serve as a method to detect the damage of autonomic nerves function in patients with chronic N-hexane poisoning.

Adolescent ; Adult ; Autonomic Pathways ; physiopathology ; Case-Control Studies ; Female ; Galvanic Skin Response ; Hexanes ; poisoning ; Humans ; Male ; Neural Conduction ; Occupational Diseases ; physiopathology ; Skin ; innervation ; physiopathology ; Sympathetic Nervous System ; physiopathology ; Young Adult

The paper adopted the JEM-100CX II transmission electron microscope to observe chloroplast ultrastructure of five kinds of dandelion (Taraxacum) leaves in northeast, and the LI-6400 portable photosynthesis system was used to compare the chlorophyll fluorescence and the photosynthesis characteristics of five kinds of dandelions in Northeast China. Chloroplast ultrastructure showed: in the five kinds of dandelion, larger chloroplast, grana with more layers, regular thylakoid, without starch grains and so on, these chloroplasts characteristics decided to bigger photosynthetic rate. The five kinds of dandelion P(n) exhibited a "double peak" diurnal curve: stomatal limitation is the main adjustment factors for the midday depression phenomenon. The P(n),G(s),C(i) content of T. mongolicum are the highest, and T. asiaticum are the lowest among them. The relation between P(n) and G(s),C(i) is direct ratio, P(n) and T(r) is in an inverse proportion among the five kinds of dandelion. In addition, P(n) is positively correlated with Chla, Chlb, and the relationship with Chlb is bigger. The paper demonstrates the Mongolian dandelion photosynthetic efficiency is the highest, it is an higher photosynthetic efficiency dandelion,it provide theoretical basis for assessment and use of the resource of dandelion.
China ; Chlorophyll ; analysis ; Chloroplasts ; ultrastructure ; Photosynthesis ; Plant Leaves ; metabolism ; ultrastructure ; Taraxacum ; metabolism ; ultrastructure

BACKGROUNDLaparoendoscopic single-site surgery (LESS) approaches have been reported for treating various kidney and pelvic procedures, and are feasible and effective in selected patients. In this study, we aimed to present the initial experience and evaluate the efficacy of laparoscopic radical prostatectomy performed through a single incision using a multichannel port.

METHODSBetween July 2010 and April 2011, six patients diagnosed with early stage prostate cancer underwent LESS radical prostatectomy (RP) in our institute. A multichannel port was inserted transperitoneally through a 2-cm umbilical incision. Specially articulating and flexible laparoscopic were used. Some technical tricks and points were applied during the operation to overcome the drawbacks and reduce the difficulties of this approach. Two continuous urethrovesical sutures in both sides were performed to complete both lateral aspects of anastomosis. The two ends of the suture threads were fixed by double Lapro-Clips, instead of the difficult knot-tying.

RESULTSTotal operative time was (265 ± 43) minutes. Mean blood loss was (230 ± 65) ml. All cases were completed successfully, without conversion to open surgery or adding additional abdomen ports. No patient required a blood transfusion and no intraoperative complications occurred. The Foley catheter was removed at the 14th day (range 12th - 16th) after surgery. At the 12th week of follow-up, all patients had an undetectable prostate-specific antigen level. Two patients used 2 or 1 pad for continence daily; other patients had achieved good continence.

CONCLUSIONIn selected cases, LESS-RP is feasible and effective; these technic points and the flexible-articulating instruments are helpful to reduce the operation difficulties.

Aged ; Humans ; Laparoscopy ; methods ; Male ; Prostatectomy ; methods

BACKGROUND AND OBJECTIVEProton magnetic resonance spectroscopy (MRS) of the liver in vivo is in experimental phase. MRS observation on liver cancer after transcatheter arterial chemoembolization (TACE) has seldom been reported. This study was to investigate the value of MRS in assessing the metabolic changes of hepatocellular carcinoma (HCC) after TACE.

METHODSTwenty-five consecutive patients with pathologically-confirmed HCC received 1H MRS of all hepatic lesions using 1.5T whole body MR scanner before TACE and at 3-10 days after TACE. Choline-to-lipid (Cho/Lip), glucogen/glucose-to-lipid (Glu/Lip), and glytamine/glutamate-to-lipid (Glx/Lip) ratios were measured and analyzed statistically.

RESULTSThe Cho/Lip, Glu/Lip, and Glx/Lip ratios were 0.21 +/- 0.08, 0.11 +/- 0.05, 0.28 +/- 0.10 before TACE, respectively, and were 0.10 +/- 0.08, 0.07 +/- 0.07, 0.18 +/- 0.12 after TACE, respectively, with significant differences (P < 0.05).

CONCLUSIONSUsing MRS can evaluate the early metabolic responses of HCC to TACE.

Adult ; Aged ; Carcinoma, Hepatocellular ; metabolism ; therapy ; Chemoembolization, Therapeutic ; Choline ; metabolism ; Female ; Glucose ; metabolism ; Glutamic Acid ; metabolism ; Glutamine ; metabolism ; Glycogen ; metabolism ; Humans ; Lipids ; analysis ; Liver Neoplasms ; metabolism ; therapy ; Magnetic Resonance Spectroscopy ; methods ; Male ; Middle Aged