ObjectiveTo investigate the effects of Linggui Zhugantang on mitochondrial fission and fusion and silencing information regulator 3（Sirt3）/adenosine monophosphate dependent protein kinase （AMPK） signaling pathway in chronic heart failure （CHF） rats after myocardial infarction （MI）. MethodSD rats randomly divide into sham operation group （normal saline ，thread only without ligature）， model group （normal saline， ligation of the left anterior descending coronary artery proximal to the heart）， Linggui Zhugantang group （4.8 g·kg^-1） and Captopril group （0.002 57 g·kg^-1）， with 10 rats in each group. Administere drug continuously for 28 days. Echocardiography detected cardiac function parameters. Hematoxylin eosin （HE） staining observed the pathological changes of the heart. Immunofluorescence detected the levels of reactive oxygen species （ROS）. JC-1 detect mitochondrial membrane potential. Colorimetry measure adenosine triphosphate （ATP）， superoxide dismutase （SOD）， malondialdehyde （MDA）， mitochondrial respiratory chain complex activity （Ⅰ-Ⅳ）. TdT-mediated dUTP nick end labeling （TUNEL） staining detected the apoptosis rate of myocardial tissue. Western blot detected protein expression levels of Sirt3， phosphorylated AMPK （p-AMPK）， phosphorylated dynamic-related protein 1（p-Drp1）， mitochondrial fission protein 1（Fis1）， mitochondrial fission factor （MFF）， optic atrophy protein 1（OPA1）. ResultCompared to the sham group， the left ventricular end diastolic diameter （LVIDd） and left ventricular end systolic diameter （LVIDs） were significantly increased in model group （P<0.01）， while the left ventricular short axis shortening rate （LVFS） and left ventricular ejection fraction （LVEF） were significantly decreased （P<0.01）. There were inflammatory cell infiltration and obvious pathological injury in myocardial tissue. ROS， MDA levels and myocardial cell apoptosis rate were significantly increased （P<0.01）， SOD level， ATP content， and membrane potential were significantly decreased （P<0.01）. The activity of mitochondrial respiratory chain complexes （Ⅰ-Ⅳ） was significantly decreased （P<0.01）. Levels of p-Drp1， Fis1， MFF proteins were significantly up-regulated （P<0.01）， while Sirt3， p-AMPK， OPA1 proteins level were significantly down-regulated （P<0.01）. Compared with model group， LVIDd and LVIDs were significantly decreased （P<0.01）， LVEF and LVFS were significantly increased （P<0.01）. Inflammatory cell infiltration and pathological damage of myocardial tissue were significantly relieved. ROS， MDA levels and myocardial cell apoptosis rate were significantly decreased in Linggui Zhugantang group and Captopril group （P<0.01）， SOD level， ATP content， and membrane potential significantly increased （P<0.01）. The activity of mitochondrial respiratory chain complexes （Ⅰ-Ⅳ） increased significantly （P<0.01），and p-Drp1， Fis1， MFF protein levels were significantly down-regulated （P<0.01）， Sirt3， p-AMPK， OPA1 protein were significantly up-regulated （P<0.01）. ConclusionLinggui Zhugantang can alleviate oxidative stress and apoptosis damage of myocardial cells， maintain mitochondrial function stability， and its effect may be related to mitochondrial mitosis fusion and Sirt3/AMPK signaling pathway.

Objective To investigate the safety and efficacy of dorzagliatin combined with metformin in the treatment of type 2 diabetes mellitus (T2DM) patients with initially overweight/obesity. Methods Eighty T2DM patients with initially overweight/obesity diagnosed in the Department of Endocrinology of Jingzhou Central Hospital from October 2023 to April 2024 were selected. They were divided into control group and observation group with 40 cases in each group by random number table method. Control group was treated with metformin,observation group was treated with dorzagliatin combined with metformin. After 12 weeks of treatment,the therapeutic effects,and fasting blood glucose (FBG),2-h postprandial blood glucose (2hPG),glycosylated hemoglobin (HbA1c),homeostasis model assessment for β cell function (HOMA-β),homeostasis model assessment of insulin resistance (HOMA-IR) index,quality of life scale for patients with type 2 diabetes mellitus (DMQLS) scores,estimated glomerular filtration rate,liver function and lipid level before and after treatment were compared between two groups. The occurrence of adverse reactions during medication was recorded. Results The total effective rate of observation group was significantly higher than that of control group (95.00％ vs. 77.50％,x2=5.165,P=0.023). After treatment,FBG,2hPG,HbA1c,HOMA-IR index and body mass index of patients in both groups were significantly lower than before treatment,while HOMA-β and DMQLS scores were significantly higher than before treatment (P<0.05). FBG,2hPG,HbA1c,HOMA-IR index and body mass index of patients in observation group were significantly lower than those in control group,while HOMA-β and DMQLS scores were significantly higher than those in control group (P<0.05). There were no serious adverse reactions occurred during treatment in two groups (P>0.05). Conclusion The effect of dorzagliatin combined with metformin in the treatment of T2DM patients with initially overweight/obesity is remarkable. It can effectively control the level of blood glucose,improve the function of pancreatic islet cells,lose weight,and improve the life quality of patients. Its clinical application value is outstanding.

Objective To investigate the safety and efficacy of dorzagliatin combined with metformin in the treatment of type 2 diabetes mellitus (T2DM) patients with initially overweight/obesity. Methods Eighty T2DM patients with initially overweight/obesity diagnosed in the Department of Endocrinology of Jingzhou Central Hospital from October 2023 to April 2024 were selected. They were divided into control group and observation group with 40 cases in each group by random number table method. Control group was treated with metformin,observation group was treated with dorzagliatin combined with metformin. After 12 weeks of treatment,the therapeutic effects,and fasting blood glucose (FBG),2-h postprandial blood glucose (2hPG),glycosylated hemoglobin (HbA1c),homeostasis model assessment for β cell function (HOMA-β),homeostasis model assessment of insulin resistance (HOMA-IR) index,quality of life scale for patients with type 2 diabetes mellitus (DMQLS) scores,estimated glomerular filtration rate,liver function and lipid level before and after treatment were compared between two groups. The occurrence of adverse reactions during medication was recorded. Results The total effective rate of observation group was significantly higher than that of control group (95.00％ vs. 77.50％,x2=5.165,P=0.023). After treatment,FBG,2hPG,HbA1c,HOMA-IR index and body mass index of patients in both groups were significantly lower than before treatment,while HOMA-β and DMQLS scores were significantly higher than before treatment (P<0.05). FBG,2hPG,HbA1c,HOMA-IR index and body mass index of patients in observation group were significantly lower than those in control group,while HOMA-β and DMQLS scores were significantly higher than those in control group (P<0.05). There were no serious adverse reactions occurred during treatment in two groups (P>0.05). Conclusion The effect of dorzagliatin combined with metformin in the treatment of T2DM patients with initially overweight/obesity is remarkable. It can effectively control the level of blood glucose,improve the function of pancreatic islet cells,lose weight,and improve the life quality of patients. Its clinical application value is outstanding.

Objective To preliminary explore the feasibility of three‐dimensional transesophageal echocardiography ( 3D‐T EE) as images data source for 3D printing model by comparing the 3D‐T EE with CT of the aortic root Digital Imaging and Communications in M edicine ( DICOM ) data into 3D printing models respectively . Methods Fifteen patients w ho underwent surgical aortic valve replacement in the hospital were enrolled ,and the aortic root 3D‐T EE and CT DICOM data were obtained in perioperative . T he images were imported into M imics software to generate digital model standard tessellation language file ,and to print the aortic root models by 3D printer . T he structural morphology of both 3D‐T EE and CT models were qualitatively evaluated respectively . T he aortic annular area ,perimeter ,maximal diameter and minimal diameter of the original data , digital model , model and aortic valve replacement were quantitatively evaluated ,and the consistency of each parameter value were analyzed . T he mean diameter of 3D‐T EE and CT model were calculated . T he correlation of mean diameter with the number of replacement was analyzed . Results ①Both 3D‐TEE and CT images data were successfully printed into 3D models ,and the positive rate of aortic valve structure were 93 .3% ( 14/15) and 80 .0% ( 12/15) respectively . ②T he measured values of the aortic annular 3D‐T EE and digital model were smaller than CT ,CTdigital model and replacement ( P＜0 .05) ,and the measurement consistency among groups was high . ③ T he parameter values of 3D‐T EE model were smaller than CT model ( P ＜0 .05 ) ,and the measured values were all within the consistency range . T he mean diameters were highly correlated with the replacement values ( r ＞ 0 .95 , P ＜ 0 .05 ) . Conclusions 3D printing aortic root model based on 3D‐TEE image data is of high feasibility .

Objective: To preliminary explore the feasibility of three-dimensional transesophageal echocardiography (3D-TEE) as images data source for 3D printing model by comparing the 3D-TEE with CT of the aortic root Digital Imaging and Communications in Medicine(DICOM) data into 3D printing models respectively. Methods: Fifteen patients who underwent surgical aortic valve replacement in the hospital were enrolled, and the aortic root 3D-TEE and CT DICOM data were obtained in perioperative. The images were imported into Mimics software to generate digital model standard tessellation language file, and to print the aortic root models by 3D printer. The structural morphology of both 3D-TEE and CT models were qualitatively evaluated respectively. The aortic annular area, perimeter, maximal diameter and minimal diameter of the original data, digital model, model and aortic valve replacement were quantitatively evaluated, and the consistency of each parameter value were analyzed. The mean diameter of 3D-TEE and CT model were calculated. The correlation of mean diameter with the number of replacement was analyzed. Results: ①Both 3D-TEE and CT images data were successfully printed into 3D models, and the positive rate of aortic valve structure were 93.3% (14/15) and 80.0% (12/15) respectively. ②The measured values of the aortic annular 3D-TEE and digital model were smaller than CT, CTdigital model and replacement (P<0.05), and the measurement consistency among groups was high. ③The parameter values of 3D-TEE model were smaller than CT model (P<0.05), and the measured values were all within the consistency range. The mean diameters were highly correlated with the replacement values (r>0.95, P<0.05). Conclusions 3D printing aortic root model based on 3D-TEE image data is of high feasibility.

Objective To investigate the clinical effect and safety of Chinese medicinal liniment pourmask and smearing in the treatment of acne. Methods Two-hundred and nine cases of acne were divided into two groups: treatment group (106 cases) was treated with Chinese medicinal liniment pourmask and smearing for 1 to 4 weeks, and control group was treated with gypsum fibrosum pourmask combining chloramphenicol and salicyic acid tincture for 1 to 4 weeks. Results At 8th week the cure rate, effective rate and improved rate in the treatment group and control group were 70.75 %, 29.25 %, 0 % and 34.95 %, 42.72 %, and 22.33 % respectively. Side effects were not found in the treatment group. Ten cases (9.71 %) in control group had contact dermatitis. Conclusion Chinese medicinal liniment pourmask and smearing have a good efficacy and safety in the treatment of acne.

This paper reported that the activation of oncogenes in human fetal esopha geal epithelium treated by alternariol (AOH). It was found that NIH/3T3 cells were transformed via transfeetion of DNA extracted from human fetal esophageal epithelium which was cultured and treated by 10?g/ml AOH in a short term in vitro. The efficiency of primary loci was 0.17 focus per ?g of DNA. In the secondary transfection, the efficiency was 0.58 focus per ?g of DNA (P