Objective:To evaluate the effects of the logistic fitting algorithm in improving the calculation accuracy of low-sampled cerebral CT perfusion (CTP) and to explore the application value of this algorithm in reducing irradiation doses to cerebral CTP.Methods:Image data from 28 patients who underwent cerebral CTP were retrospectively analyzed. A total of 192 regions of interest (ROIs) were circled in the basal ganglia and the main blood-supplying areas of the anterior, middle, and posterior cerebral arteries. For each ROI, the time-density curve (TDC) was plotted, from which some data points were deleted to decrease the sampling frequency to half of the conventional scan, thus simulating low-dose scanning and obtaining low-dose TDCs. The logistic model was applied to fit and complete the low-dose TDCs. The potential decrease in radiation dose was assessed. Conventional TDCs, low-dose TDCs, and TDCs processed using the logistic fitting algorithm were compared. Perfusion calculations were performed based on these TDCs, and the calculated cerebral blood volume (CBV), cerebral blood flow (CBF), time to peak (TTP), and mean transit time (MTT) were compared and analyzed.Results:The total radiation dose for cerebral CTP examination could be reduced to 52% of the routine dose. The mean correlation coefficient R between the TDCs derived using the logistic fitting algorithm and the conventional TDCs was 0.958 ± 0.03. The CBV, CBF, TTP, and MTT calculated using the logistic fitting algorithm were compared with the conventional result, and the coefficients of determination R2 of linear regressions were determined at 0.943, 0.942, 0.955, and 0.891, respectively, indicating extremely high consistency(ICC > 0.90). Furthermore, the R2 values determined using the logistic fitting algorithm were all higher than those derived without applying the fitting algorithm. Conclusions:Applying the logistic fitting algorithm to under-sampled cerebral CTP can yield calculation result that are highly consistent with those of conventional sampling. By combining under-sampling with the logistic algorithm, the irradiation doses can be reduced while guaranteeing the accuracy of the effective perfusion result, demonstrating high application value.

Objective:To evaluate the effects of the logistic fitting algorithm in improving the calculation accuracy of low-sampled cerebral CT perfusion (CTP) and to explore the application value of this algorithm in reducing irradiation doses to cerebral CTP.Methods:Image data from 28 patients who underwent cerebral CTP were retrospectively analyzed. A total of 192 regions of interest (ROIs) were circled in the basal ganglia and the main blood-supplying areas of the anterior, middle, and posterior cerebral arteries. For each ROI, the time-density curve (TDC) was plotted, from which some data points were deleted to decrease the sampling frequency to half of the conventional scan, thus simulating low-dose scanning and obtaining low-dose TDCs. The logistic model was applied to fit and complete the low-dose TDCs. The potential decrease in radiation dose was assessed. Conventional TDCs, low-dose TDCs, and TDCs processed using the logistic fitting algorithm were compared. Perfusion calculations were performed based on these TDCs, and the calculated cerebral blood volume (CBV), cerebral blood flow (CBF), time to peak (TTP), and mean transit time (MTT) were compared and analyzed.Results:The total radiation dose for cerebral CTP examination could be reduced to 52% of the routine dose. The mean correlation coefficient R between the TDCs derived using the logistic fitting algorithm and the conventional TDCs was 0.958 ± 0.03. The CBV, CBF, TTP, and MTT calculated using the logistic fitting algorithm were compared with the conventional result, and the coefficients of determination R2 of linear regressions were determined at 0.943, 0.942, 0.955, and 0.891, respectively, indicating extremely high consistency(ICC > 0.90). Furthermore, the R2 values determined using the logistic fitting algorithm were all higher than those derived without applying the fitting algorithm. Conclusions:Applying the logistic fitting algorithm to under-sampled cerebral CTP can yield calculation result that are highly consistent with those of conventional sampling. By combining under-sampling with the logistic algorithm, the irradiation doses can be reduced while guaranteeing the accuracy of the effective perfusion result, demonstrating high application value.

A 58-year-old male patient took Weikening tablets 1.24 g thrice daily orally for gastric ulcer by himself. The patient did not take other drugs during the same period. After 1 month of medication, the patient developed the symptoms such as abdominal distension, yellowish sclera, yellowish urine, and fatigue. Laboratory tests showed alanine aminotransferase (ALT) 1 272 U/L, aspartate aminotransferase (AST) 507 U/L, total bilirubin (TBil) 59 μmol/L, and direct bilirubin (DBil) 34 μmol/L. Acute liver injury caused by Weikening tablets was considered. Then the drug was stopped and treatments such as liver protective, choleretic, and enzyme reduction drugs were given. Ten days later, the above symptoms disappeared, and the liver function reexamination showed ALT 163 U/L, AST 43 U/L, TBil 23 μmol/L, and DBil 17 μmol/L. One month later, the patient′s liver function test showed ALT 31 U/L, AST 24 U/L, TBil 14 μmol/L, and DBil 6 μmol/L. It was considered that the liver injury of the patient may be related to greater celandine contained in Weikening tablets.

A 58-year-old male patient took Weikening tablets 1.24 g thrice daily orally for gastric ulcer by himself. The patient did not take other drugs during the same period. After 1 month of medication, the patient developed the symptoms such as abdominal distension, yellowish sclera, yellowish urine, and fatigue. Laboratory tests showed alanine aminotransferase (ALT) 1 272 U/L, aspartate aminotransferase (AST) 507 U/L, total bilirubin (TBil) 59 μmol/L, and direct bilirubin (DBil) 34 μmol/L. Acute liver injury caused by Weikening tablets was considered. Then the drug was stopped and treatments such as liver protective, choleretic, and enzyme reduction drugs were given. Ten days later, the above symptoms disappeared, and the liver function reexamination showed ALT 163 U/L, AST 43 U/L, TBil 23 μmol/L, and DBil 17 μmol/L. One month later, the patient′s liver function test showed ALT 31 U/L, AST 24 U/L, TBil 14 μmol/L, and DBil 6 μmol/L. It was considered that the liver injury of the patient may be related to greater celandine contained in Weikening tablets.

Objective:To understand the current situation of scientific research ability and training needs of community nurses in China.Methods:The convenient sampling method was used to select 3 633 community nurses from 16 provinces/autonomous regions/municipalities from June to August 2021 to investigate. The contents of the questionnaire included general information, Self-Assessment Scale of Scientific Research Ability for Nursing Staff, scientific research attitude and training needs. A total of 3 633 questionnaires were recovered, of which 3 561 were valid, with an effective recovery rate of 98.0%. Univariate analysis and multiple linear regression analysis were used to analyze the influencing factors of the total score of self-assessment of scientific research ability of community nurses.Results:The total score of scientific research ability of 3 561 community nurses was 39.00 (28.00, 60.00) . The results of multiple linear regression analysis showed that nursing working years, highest education, received research courses/training, working experience in academic organizations/academic journals, participating in academic conferences or above the municipal level in the past 5 years and having plans to improve academic qualifications in the next 5 years were the influencing factors of scientific research ability of community nurses ( P<0.05) , jointly explained 15.4% of the total variance (adjusted R2=0.154, P<0.001) . Community nurses had training needs in data collection and organization, scientific research design, literature retrieval, scientific research topic selection, data statistical analysis and statistical software operation and submission skills. Conclusions:Subjective self-assessment scientific research ability and objective scientific research output of community nurses need to be improved and key scientific research talents need to be cultivated.

Objective To study the mechanism of brain death-induced heart damage by observing the change patterns of morphological damage to the heart and related inflammatory factors after brain death and provide the experimental basis for heart transplantation by brain-dead donor.Methods The 30 rabbits were equally divided into two groups by the random digital table method:sham-operation group and brain death group.The rabbit brain death model was established in the brain death group,and the sham-operation group was given slow intracranial pressure.The rest treatments in the two groups were the same.At 2nd,6th and 8th h after operation,blood pressure,heart rate and respiratory rate were recorded.The damage of heart tissues was observed by HE staining.The plasma concentrations of IL-1,IL-6 and IL-8 were tested by ELISA.The expression of some inflammatory factors in heart issues was detected by RT-PCR and immunohistochemistry.Results At 8 h after brain death,there was no signifiant difference in blood pressure and heart rate between two groups (P＞0.05).The damage of heart issues in the brain death group was more serious than in the shamoperation group.With the prolongation of brain death,the plasma concentrations of IL-6 and IL-8 increased significantly in the brain death group (P＜0.05),but the concentration of IL-1β showed no siginificant difference between the two groups at 2 h after brain death (P＜0.05).Besides,the expression of HSP27 and HSP70 mRNA as well as the protein expression of ICAM and NF-κB was significantly increased in the brain death group as compared with that in the sham-operation group (P＜0.05).Conclusion With the prolongation of brain death time,the inflammatory factors in the heart tissues and plasma interleukin were increased,suggesting the inflammatory reaction occurs in donor heart under the condition of brain death,which influences the quality of donor in the heart transplantation.

Objective To verify the performance of LIAISON chemiluminescence immunoassay analyzer in the prenatal screening for TORCH .Methods Reference to the US Institute of Clinical and Laboratory Stand-ards(NCCLS) series of documents and literature and combining with actual work ,we designed the verification program ,and tested and evaluated the LIAISON chemiluminescent immunoassay systems for the measurement precision ,accuracy ,linearity analysis ,clinical reportable range and biological reference intervals of Tox IgG , Tox IgM ,Rub IgG ,Rub IgM ,CMV IgG ,CMV IgM ,HSV IgG ,HSV IgM .We also compared the results with analysis performance provided by manufacturers (Italy LIAISON ) or recognized quality indicators .Results Intra-assay imprecision CV values were between 3 .58％ -7 .03％ ,which were less than the predetermined range;inter-assay imprecision CV values were between 3 .13％ -10 .73％ .Linear range validation regression coefficients a values were between 0 .97 -1 .03 and r2 >0 .95 .The linear relationship met the requirements . Both biological reference interval and reportable range meet the requirements .Conclusion The performance of LIAISON chemiluminescence immunoassay detection system satisfied the clinical requirements ,and the meas-urement results had advantages of high sensitivity ,specificity ,stability ,wide detection range ,good accuracy and repeatability ,which was suitable for clinical application .

Regulating phase Ⅰ and Ⅱ enzyme,drug transporters and constitutive androstane receptor (CAR) play an important role in dug metabolism and keeping balance on the levels of glucose,lipids and hormone in serum.As a hub of material metabolism within body,CAR can affect the efficacy of various drugs and be involved in many common metabolic diseases.Studying its polymorphism is referential for explaining the individual difference genetically and predicting the occurrence and development of diseases.This review focuses on the correlation between the CAR polymorphism and metabolic,which provides evidence for predicting the development of disease and choosing clinical drug dosage.

Objective To compare the purification process of two types of ceramic hydroxyapatite(CHT I and CHT II)to remove the aggregates from two monoclonal antibodies(mAb 1 and mAb 2).Methods All the chromatography runs were performed on AKTA AVANT 150 with Tricon 10/50 column.The dynamic binding capacity( DBC) of two types of CHT was studied firstly, and then purification research was carried out selecting the suitable DBC.The column was equilibrated with 5 mmol/L sodium dihydrogen phosphate pH 6.5, and then was eluted with gradient buffers which were 10 mmol/L sodium dihydrogen phosphate pH 6.5 and 2 mol/L sodium chloride pH 6.5.Aggregate content in loading and elution pool was evaluated by size exclusion chromatography.Scale-up process was carried on 20 cm height chromatography column XK16/40.Results DBC of CHT I for mAb 1 was 40 mg/mL and mAb 2 was 45 mg/mL.After purity, monomer content of mAb 1 reached 98.6% and yield was 92.5% and monomer content of mAb 2 reached 98.8%and yield was 91.5%.DBC of CHT II for mAb1 was 16 mg/mL and mAb 2 was 20 mg/mL.After purity, monomer content of mAb 1 reached 99.8% and yield was 91.8% and monomer content of mAb 2 reached 99.9% and yield was 92.2%.Conclusion Two types of CHT both can remove aggregates effectively from monoclonal antibodies when aggregate content reaches more than 10%, and results conform to the regulations.CHT I has higher dynamic binding capacity than CHT II, and CHT II is superior to CHT I in removing aggregate efficiency.The purification process is simple and can be easily scaled up in pilot and manufacture.Therefore, it meets the requirement pilot and scale production.

To investigate the prevalence and risk factors of stress urinary incontinence in women of Miyun County.Questionnaire survey was conducted in 8665 residents selected from urban and rural areas of Miyun County.Total 8663 questionnaires were completed with a response rate of 99.98％.The prevalence of urinary incontinence was 34.43％ (2983/8663),among which 1790 cases were of stress type with a prevalence rate of 20.66％,414 cases were of urgent type with a prevalence rate of 4.78％ and 779 cases were of mixed type with a prevalence rate of 8.99％.The risk factors of the stress urinary incontinence were advanced age(OR =1.555),BMI≥24 kg/m2 (OR =0.556),alcohol drinking(OR =1.308),constipation (OR =1.360),delivery times≥3(OR =1.998),history of macrosomia-bearing (OR =0.572).