OBJECTIVE To analyze the expression levels of peripheral blood long non-coding RNA nuclear-enriched abundant transcript 1(lncRNA NEAT1),Toll-like receptor 2(TLR2)and nuclear factor kappa B(NF-κB)in pa-tients with acute respiratory tract infection and their relationship with helper T cell(Th)1/Th2 imbalance.METHODS The clinical data of 120 patients with emergency respiratory tract infection admitted to Changzhou Sec-ond People's Hospital Affiliated to Nanjing Medical University between Jan.2022 and Jan.2024 were retrospec-tively analyzed,the patients were set as study group(n=120),and 70 healthy volunteers who came to the hospi-tal for physical examination during the same period were served as healthy group(n=70).The levels of lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,CD4+Th1,CD4+Th2 and Th1/Th2 in peripheral blood,and expression levels of Th1/Th2 cell immune homeostasis-associated factors[interferon-γ(IFN-γ),interleukin-4(IL-4)and IFN-γ/IL-4]were compared between the two groups of subjects,Pearson correlation analysis was applied to ex-plore the correlation between the levels of peripheral blood lncRNA NEAT1,TLR2 mRNA and NF-κB mRNA and Th1/Th2 balance and its cellular immune homeostasis-associated cytokines in patients with emergency respira-tory tract infection,and receiver operating characteristic(ROC)curve was used to analyze the predictive efficiency of peripheral blood lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,Th1/Th2 and IFN-γ/IL-4 for acute respira-tory tract infection.RESULTS The pathogens detected in the study group were predominantly Streptococcus pneu-moniae(30.30％).The levels of peripheral blood lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,CD4+Th2 and IL-4 in study group were(1.64±0.40),(0.85±0.21),(0.69±0.15),(8.82±2.40)×10-2 and(25.48±6.77)pg/ml,a little higher than those in healthy group,while the levels of CD4+Th1,Th1/Th2,IFN-γ and IFN-γ/IL-4 were(6.87±2.05)×10-2,(0.86±0.45),(52.67±11.89)pg/ml and(2.25±0.94),lower than those in the healthy group(all P＜0.05).Pearson correlation analysis showed that the peripheral blood lncRNA NEAT1,TLR2 mRNA and NF-κB mRNA of subjects with acute respiratory tract infection were negatively corre-lated with Th1/Th2(r=-0.445,-0.316,-0.337,all P＜0.05)and IFN-γ/IL-4(r=-0.454,-0.308,-0.375,all P＜0.05).ROC curve results showed that peripheral blood lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,Th1/Th2 and IFN-γ/IL-4 had high diagnostic efficiency for respiratory tract infection[the area under the curve(AUG)=0.887,0.924,0.934,0.935 and 0.957 respectively,all P＜0.05].CONCLUSION Peripheral blood lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,Th1/Th2 and IFN-γ/IL-4 levels have high diagnostic val-ue for acute respiratory tract infection,and lncRNA NEAT1 levels are closely related to TLR2 mRNA,NF-κB mRNA,Th1/Th2 and IFN-γ/IL-4 levels.

OBJECTIVE To analyze the expression levels of peripheral blood long non-coding RNA nuclear-enriched abundant transcript 1(lncRNA NEAT1),Toll-like receptor 2(TLR2)and nuclear factor kappa B(NF-κB)in pa-tients with acute respiratory tract infection and their relationship with helper T cell(Th)1/Th2 imbalance.METHODS The clinical data of 120 patients with emergency respiratory tract infection admitted to Changzhou Sec-ond People's Hospital Affiliated to Nanjing Medical University between Jan.2022 and Jan.2024 were retrospec-tively analyzed,the patients were set as study group(n=120),and 70 healthy volunteers who came to the hospi-tal for physical examination during the same period were served as healthy group(n=70).The levels of lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,CD4+Th1,CD4+Th2 and Th1/Th2 in peripheral blood,and expression levels of Th1/Th2 cell immune homeostasis-associated factors[interferon-γ(IFN-γ),interleukin-4(IL-4)and IFN-γ/IL-4]were compared between the two groups of subjects,Pearson correlation analysis was applied to ex-plore the correlation between the levels of peripheral blood lncRNA NEAT1,TLR2 mRNA and NF-κB mRNA and Th1/Th2 balance and its cellular immune homeostasis-associated cytokines in patients with emergency respira-tory tract infection,and receiver operating characteristic(ROC)curve was used to analyze the predictive efficiency of peripheral blood lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,Th1/Th2 and IFN-γ/IL-4 for acute respira-tory tract infection.RESULTS The pathogens detected in the study group were predominantly Streptococcus pneu-moniae(30.30％).The levels of peripheral blood lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,CD4+Th2 and IL-4 in study group were(1.64±0.40),(0.85±0.21),(0.69±0.15),(8.82±2.40)×10-2 and(25.48±6.77)pg/ml,a little higher than those in healthy group,while the levels of CD4+Th1,Th1/Th2,IFN-γ and IFN-γ/IL-4 were(6.87±2.05)×10-2,(0.86±0.45),(52.67±11.89)pg/ml and(2.25±0.94),lower than those in the healthy group(all P＜0.05).Pearson correlation analysis showed that the peripheral blood lncRNA NEAT1,TLR2 mRNA and NF-κB mRNA of subjects with acute respiratory tract infection were negatively corre-lated with Th1/Th2(r=-0.445,-0.316,-0.337,all P＜0.05)and IFN-γ/IL-4(r=-0.454,-0.308,-0.375,all P＜0.05).ROC curve results showed that peripheral blood lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,Th1/Th2 and IFN-γ/IL-4 had high diagnostic efficiency for respiratory tract infection[the area under the curve(AUG)=0.887,0.924,0.934,0.935 and 0.957 respectively,all P＜0.05].CONCLUSION Peripheral blood lncRNA NEAT1,TLR2 mRNA,NF-κB mRNA,Th1/Th2 and IFN-γ/IL-4 levels have high diagnostic val-ue for acute respiratory tract infection,and lncRNA NEAT1 levels are closely related to TLR2 mRNA,NF-κB mRNA,Th1/Th2 and IFN-γ/IL-4 levels.

OBJECTIVE To study the improvement effects of Shaoyao gancao decoction （SGD） on acute lung injury （ALI） in rats and its effects on the intestinal flora. METHODS Sixty SD rats were randomly divided into normal group （CON group， normal saline）， model group （MOD group， normal saline）， positive control group （DEX group， 5 mg/kg dexamethasone）， SGD low-dose， medium-dose and high-dose groups （SGD-L， SGD-M， SGD-H groups， 5.8， 11.6， 23.2 g/kg， calculated by crude drug）， with 10 rats in each group. Each group was given relevant medicine 10 mL/kg intragastrically， for 7 consecutive days. Thirty minutes after the last administration， CON group was given constant volume of normal saline via airway infusion， and other groups were given lipopolysaccharide （5 mg/kg） via airway infusion to induce ALI model. After 12 hours of modeling， the lung tissue wet/dry weight ratio was calculated， and the contents of interleukin 1β （IL-1β）， IL-6 and tumor necrosis factor α（TNF-α） in rat bronchial alveolar lavage fluid （BALF） were all detected； the pathological changes of lung tissue were observed after hematoxylin-eosin staining. The intestinal flora of rat feces was analyzed by 16S rRNA sequencing technology， and the correlation of differential bacteria genera with inflammatory factors was also analyzed. RESULTS Compared with MOD group， the infiltration of inflammatory cells in the lung tissue of rats in each SGD dose group was decreased， and the thickening of alveolar septum and pulmonary edema improved； lung tissue wet/dry weight ratio， the levels of IL-1β， IL-6 and TNF-α in BALF significantly decreased （P＜0.05 or P＜0.01）. SGD （low dose） could improve the intestinal flora disorder in ALI rats， restore the diversity and richness of intestinal flora， regulate the structure of flora， reduce the abundance of Lactobacillus， Streptococcus and Escherichia-Shigella， and increase the abundance of Firmicutes， Lachnospira， Ruminococcus， Clostridia，Dubosiella and Akkermansia. Through correlation analysis， it was found that the relative abundance of Lactobacillus， Streptococcus and Escherichia-Shigella was positively related to the levels of inflammatory factor IL-1β， IL-6 and TNF-α （P＜0.05 or P＜0.01）. The relative abundance of Lachnospira， Dubosiella， Firmicutes was significantly negatively correlated with the levels of inflammatory factors mentioned above （P＜0.05 or P＜0.01）. CONCLUSIONS SGD may improve ALI by reducing lung tissue injury and inflammatory response and regulating flora structure in rats.

OBJECTIVE To study the “property-effect” correlation material basis of the classic famous prescription Taohe chengqi decoction in removing blood stasis and purging heat. METHODS The rat model of blood storage syndrome was established to investigate the effects of Taohe chengqi decoction （17.29 g/kg） and its property grouping components [bitter-cold property group （Rhei Radix et Rhizoma+Natrii Sulfas） 9.43 g/kg， pungent-warm property group （Cinnamomi Ramulus+Persicae Semen） 4.71 g/kg， sweet-flat property group （Glycyrrhizae Radix et Rhizoma） 3.14 g/kg] on coagulation indexes （prothrombin time， prothrombin activity， activated partial thrombin time， fibrinogen， thrombin time and endothelin-1） and inflammation indicators （C- reactive protein， tumor necrosis factor-α and superoxide dismutase） in rats. On the basis of determining the dominant property groups， ultra-performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry was used to identify the components. The molecular docking was performed， and the components with binding energy ≤－5.0 kcal/mol were selected as the basis of “property-effect” correlation material basis. RESULTS Taohe chengqi decoction total prescription group and pungent-warm property group could significantly improve the coagulation indexes of model rats （P＜0.05 or P＜0.01）， and the total prescription group and bitter-cold property group could significantly improve the inflammation indexes of model rats （P＜0.05 or P＜0.01）. The pungent-warm property group was the dominant property group for removing blood stasis，and the bitter-cold property group was the dominant property group for purging heat. Thirty-two and thirty-five components were identified from the pungent-warm property group and bitter-cold property group， respectively. With binding energy ≤－5.0 kcal/mol， there were 10 components （abscisic acid， 3，4-dihydroxypheny- lethanol， procyanidin B1， etc.） in the pungent-warm property group and 13 components （baicalein， aloe-emodin， demethylwedelolactone， etc.） in the bitter-cold property group. CONCLUSIONS Taohe chengqi decoction has the effect of removing blood stasis and purging heat， and its material basis comes from pungent-warm property group （Cinnamomi Ramulus， Persicae Semen） and bitter-cold property group （Rhei Radix et Rhizoma， Natrii Sulfas） respectively.

Objective:To identify the clinical significance of CDK5 in colon cancer tissues.Methods:Two hundred colon cancer tissues were tested for CDK5 expression by immunohistochemistry on tissue microarrays. The correlation between CDK5 expression and clinicopathological features, prognosis and peripheral inflammation-related cells was analyzed.Results:CDK5 was low expressed in 100 cases (50.0%), and high in another 100 cases (50.0%). Longer time to tumor progression ( P=0.026) and overall survival ( P=0.035) were observed in patients with high CDK5 expression. By multivariate analysis , the expression of CDK5 was an independent risk factor for poor prognosis ( HR=0.45,95% CI: 0.21-0.99, P=0.049). The expression of CDK5 was not related to the counts of white blood cells and neutrophils ( P>0.05). Prognosis of patients with a positive lymph node ratio less than 0.15 was significantly better than that of patients with a higher lymph node ratio ( P<0.001). Conclusions:Patients with low CDK5 expression have poor prognosis, and CDK5 expression is not related to the counts of peripheral white blood cells and neutrophils.

Objective To investigate the association of serum levels of matrix metalloproteinase-12 (MMP-12) and a functional polymorphism in the promoter - 82A/G of the MMP-12 gene with acute atherosclerotic cerebral infarction (ACI).Methods All 608 cascs of acute ACI and 374 healthy controls were included in the study.Serum levels of MMP-12 were measured using the enzyme-linked immunosorbent assay (ELISA). At the same time,polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed on the - 82A/G polymorphism in the MMP-12 gene.Results The serum levels of MMP-12 in ACI group (( 17.36 ± 9.12) ng/ml) were not significantly higher than those in the healthy control group ( ( 17.42 ± 7.70) ng/ml,t ＝ 0.047,P =0.962).The frequency of the AG + GG genotypes was not significantly different between the two groups (7.6％ vs 5.9％,x2 =0.281,P =0.584),and the frequencies of the G allele were 3.8％ and 2.9％ in the ACI group and the control group respectively which were also not significantly different between the two groups ( x2 =0.746,P =0.374).Conclusion There is no correlation between serum level with genetic polymorphism in MMP-12 promoter (-82A/G) and cerebral infarction.

Objective To explore the methods of cultivating 7-year-program medical students' clinical practice ability and scientific research ability in gynecology. Methods We carried out a training system,in which clinical groups and scientific research groups were guided by tutors,assisted with strict examining measures and reasonable incentive mechanisms. Re-sults According to a practice cycle of 2 years,medical students'overall quality was improved obviously,such as clinical thoughts,basic skills,humanistic qualities,scientific research thoughts and the ability of literature retrieval. Also the teaching enthusiasm and teaching level of the teaching group were improved corresponding. Conclusion Construction and implementation of the training system of clinical groups and scientific research groups under the leadership of the tutors is obviously beneficial to the overall quality of medical students and the integrated teaching capabilities of the professional teaching team.