Objective:To establish a method for the quality evaluation of zhibitai capsules by high performance liquid chromatography-quantitative analysis of multi-components by single marker(HPLC-QAMS)combined with chemometrics.Methods:HPLC method was used to establish the relative correction factors(f)of alisol A with vitexin-4"-O-glucoside,rhamnosylvitexin,oleanolic acid,ursolic acid,atractylenolide Ⅲ,atracylenolide Ⅰ,alisol A 24-acetate and 23-acetate alisol B by external standard method with alisol A as the internal reference.The estab-lished f was used to calculate the contents of the corresponding eight components,and the differences between the measured values and the calculated values were compared.The content data were analyzed by chemometrics to evaluate its quality.Results:The f values of alisol A with vitexin-4"-O-glucoside,rhamnosylvitexin,oleanolic acid,ursolic acid,atractylenolide Ⅲ,atracylenolide Ⅰ,alisol A 24-acetate and 23-acetate alisol B were 1.044 0,0.633 1,0.861 0,0.691 7,1.224 8,1.372 7,1.516 1 and 0.768 2,respectively,and the repeatability was good.Nine components in 13 batches of zhibitai capsules were determined by QAMS method and external standard method,and the results were not significantly different.The results of principal component analysis showed that 13 batches of samples were clustered into 3 categories.The main quality difference factors of zhibitai capsule were rhamnosylvitexin,ursolic acid,atractylenolide Ⅲ,atracylenolide Ⅰ and 23-acetyl alisol B.Conclusion:The estab-lished method can be used for the comprehensive evaluation of the quality of zhibitai capsules.

Objective To experimentally verify the precision and accuracy of determining tritiated water in ambient air using the desiccant adsorption sampling–high temperature negative pressure desorption of liquid water (containing HTO)–liquid Scintillation counter method, and to provide technical support for developing standard methods for monitoring tritiated water in ambient air. Methods The relative standard deviation and recovery of multi-group samples were verified by collecting, testing, and analyzing environmental samples with different activity concentrations. The uncertainty of the method was evaluated, the main uncertainty components were identified, and the reliability of measurement results was analyzed. Through experimental comparison of different methods, the differences in the test results of different methods were examined. Results The relative standard deviation of multiple samples ranged from 6.7% to 7.9%, the recovery ranged from 95.7% to 97.3%, and the uncertainty was greatly affected by the sample counting rate, with no significant difference as compared to condensation sampling method. Conclusion The precision and accuracy of this method meet the requirements of environmental authorities for monitoring tritiated water in ambient air, and it can be widely used in the monitoring of tritiated water in ambient air.

Objective To explore the method for measurement of tritium in nuclear power plant liquid effluent purified by a mixed ion-exchange resin, to verify the feasibility of the method by experiments, and to provide technical support for the development of standard methods for the measurement of tritium in liquid effluent of nuclear power plants. Methods The purification effect of the mixed ion-exchange resin was determined by measuring the quenching factor, conductivity, and β-nuclide adsorption efficiency of the samples purified using the resin. A comparison was made between the ion-exchange resin method and the atmospheric distillation method for tritium determination. The precision and accuracy of the method were verified by calculating the relative standard deviation and the recovery in repeated measurement of samples with different activity concentrations and spiked samples. Results There were no significant differences in quenching factor, conductivity, and tritium activity concentration between the two methods. The adsorption efficiencies of EC20MB resin for common β-nuclides such as carbon, iron, nickel, strontium, yttrium, and cesium ranged from 99.28% to 99.88%. Repeated measurement of the same sample showed relative standard deviations of 5.2%-9.4% and recoveries of 86.8%-107%. Conclusion There were no significant differences between the results of the ion-exchange resin purification method and the atmospheric distillation method. The precision and accuracy of the method met the requirements of the ecological and environmental management authorities for monitoring tritium in liquid effluent from nuclear power plants. This method can be widely used in daily monitoring work.

Objective:To establish a method for the quality evaluation of zhibitai capsules by high performance liquid chromatography-quantitative analysis of multi-components by single marker(HPLC-QAMS)combined with chemometrics.Methods:HPLC method was used to establish the relative correction factors(f)of alisol A with vitexin-4"-O-glucoside,rhamnosylvitexin,oleanolic acid,ursolic acid,atractylenolide Ⅲ,atracylenolide Ⅰ,alisol A 24-acetate and 23-acetate alisol B by external standard method with alisol A as the internal reference.The estab-lished f was used to calculate the contents of the corresponding eight components,and the differences between the measured values and the calculated values were compared.The content data were analyzed by chemometrics to evaluate its quality.Results:The f values of alisol A with vitexin-4"-O-glucoside,rhamnosylvitexin,oleanolic acid,ursolic acid,atractylenolide Ⅲ,atracylenolide Ⅰ,alisol A 24-acetate and 23-acetate alisol B were 1.044 0,0.633 1,0.861 0,0.691 7,1.224 8,1.372 7,1.516 1 and 0.768 2,respectively,and the repeatability was good.Nine components in 13 batches of zhibitai capsules were determined by QAMS method and external standard method,and the results were not significantly different.The results of principal component analysis showed that 13 batches of samples were clustered into 3 categories.The main quality difference factors of zhibitai capsule were rhamnosylvitexin,ursolic acid,atractylenolide Ⅲ,atracylenolide Ⅰ and 23-acetyl alisol B.Conclusion:The estab-lished method can be used for the comprehensive evaluation of the quality of zhibitai capsules.