OBJECTIVE: To explore the efficacy and apoptosis of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in the treatment of acute myeloid leukemia (AML) with ASXL1 mutation. METHODS: The clinical data of 80 AML patients with ASXL1 mutation treated in our hospital from January 2019 to December 2021 were retrospectively analyzed. The clinical characteristics of the patients were summarized, and the therapeutic effect and prognostic factors of allo-HSCT for the patients were analyzed. RESULTS: Among the 80 patients, 38 were males and 42 were females, and the median age was 39(14-65) years. There were 17 patients in low-risk group, 25 patients in medium-risk group and 38 patients in high-risk group. ASXL1 mutation co-occurred with many other gene mutations, and the frequent mutated genes were TET2 (71.25%), NRAS (18.75%), DNMT3A (16.25%), NPM1 (15.00%), CEBPA (13.75%). Among medium and high-risk patients, 29 underwent allo-HSCT, while 34 received chemotherapy. The 2-year overall survival (OS) rate and disease-free survival (DFS) rate of the allo-HSCT group were 72.4% and 70.2%, while those of the chemotherapy group were 44.1% and 34.0%, respectively. The statistical analysis showed significant differences between the two groups (both P < 0.01). Multivariate analysis showed that age at transplantation >50- years and occurrence of acute graft-versus-host disease after transplantation were poor prognostic factors for OS and DFS in transplantation patients. CONCLUSION Allo-HSCT can improve the prognosis of AML patients with ASXL1 mutation.
Humans ; Leukemia, Myeloid, Acute/therapy* ; Hematopoietic Stem Cell Transplantation ; Female ; Male ; Middle Aged ; Mutation ; Adult ; Repressor Proteins/genetics* ; Adolescent ; Retrospective Studies ; Aged ; Nucleophosmin ; Young Adult ; Transplantation, Homologous ; Prognosis ; Survival Rate

Objective To explore the mechanism of cinobufotalin regulating phosphatase and tensin homolog(PTEN)and member of wingless MMTV integration site family(Wnt)signaling pathway through microRNA-21(miR-21)on non-small cell lung cancer(NSCLC)cells.Methods HCC827 cells were divided into control group(normal culture),inhibitor-NC group(transfected with miR-21 inhibitor negative control),miR-21 inhibitor group(transfected with miR-21 inhibitor),cinobufacini(Cin)group(0.15 μg·mL-1 cinobufacini),miR-21 mimics group(0.15 μg·mL-1 cinobufacini+miR-21 mimics),OE-PTEN group(0.15 μg·mL-1 cinobufacini+miR-21 mimics+overexpression PTEN plasmid).The mRNA expression levels programmed death ligand-1(PD-L1)were detected by quantitative real-time polymerase chain reaction(qRT-PCR);the number of cell invasion was detected by Transwell chamber method;the expression levels of Wnt/β-catenin pathway-related proteins were detected by Western blot.Results The number of cell invasion in the control group,Cin group,miR-21 mimics group and OE-PTEN group were 121.16±16.43,68.56±11.21,149.30±21.26 and 96.13±13.31,respectively;the protein expression levels of Wnt4 were 1.00±0.13,0.45±0.06,0.93±0.12 and 0.55±0.08,respectively;the protein expression levels ofβ-catenin were 1.00±0.14,0.26±0.04,0.82±0.10 and 0.15±0.03;the mRNA expression levels of PD-L1 were 1.00±0.08,0.71±0.09,1.21±0.11 and 0.70±0.13,respectively.The above indicators of the control group were compared with the Cin group,the above indicators of the Cin group were compared with the miR-21 mimics group,and the above indicators of the miR-21 mimics group were compared with the OE-PTEN group,the differences were statistically significant(all P＜0.05).Conclusion Cinobufotalin may improve the immune function of NSCLC cells,inhibit their invasion and proliferation by down-regulating miR-21 expression to regulate PTEN,Wnt/β-catenin pathway.

Objective To explore the mechanism of cinobufotalin regulating phosphatase and tensin homolog(PTEN)and member of wingless MMTV integration site family(Wnt)signaling pathway through microRNA-21(miR-21)on non-small cell lung cancer(NSCLC)cells.Methods HCC827 cells were divided into control group(normal culture),inhibitor-NC group(transfected with miR-21 inhibitor negative control),miR-21 inhibitor group(transfected with miR-21 inhibitor),cinobufacini(Cin)group(0.15 μg·mL-1 cinobufacini),miR-21 mimics group(0.15 μg·mL-1 cinobufacini+miR-21 mimics),OE-PTEN group(0.15 μg·mL-1 cinobufacini+miR-21 mimics+overexpression PTEN plasmid).The mRNA expression levels programmed death ligand-1(PD-L1)were detected by quantitative real-time polymerase chain reaction(qRT-PCR);the number of cell invasion was detected by Transwell chamber method;the expression levels of Wnt/β-catenin pathway-related proteins were detected by Western blot.Results The number of cell invasion in the control group,Cin group,miR-21 mimics group and OE-PTEN group were 121.16±16.43,68.56±11.21,149.30±21.26 and 96.13±13.31,respectively;the protein expression levels of Wnt4 were 1.00±0.13,0.45±0.06,0.93±0.12 and 0.55±0.08,respectively;the protein expression levels ofβ-catenin were 1.00±0.14,0.26±0.04,0.82±0.10 and 0.15±0.03;the mRNA expression levels of PD-L1 were 1.00±0.08,0.71±0.09,1.21±0.11 and 0.70±0.13,respectively.The above indicators of the control group were compared with the Cin group,the above indicators of the Cin group were compared with the miR-21 mimics group,and the above indicators of the miR-21 mimics group were compared with the OE-PTEN group,the differences were statistically significant(all P＜0.05).Conclusion Cinobufotalin may improve the immune function of NSCLC cells,inhibit their invasion and proliferation by down-regulating miR-21 expression to regulate PTEN,Wnt/β-catenin pathway.

Platelets are reprogrammed by cancer via a process called education, which favors cancer development. The transcriptional profile of tumor-educated platelets (TEPs) is skewed and therefore practicable for cancer detection. This intercontinental, hospital-based, diagnostic study included 761 treatment-naïve inpatients with histologically confirmed adnexal masses and 167 healthy controls from nine medical centers (China, n = 3; Netherlands, n = 5; Poland, n = 1) between September 2016 and May 2019. The main outcomes were the performance of TEPs and their combination with CA125 in two Chinese (VC1 and VC2) and the European (VC3) validation cohorts collectively and independently. Exploratory outcome was the value of TEPs in public pan-cancer platelet transcriptome datasets. The AUCs for TEPs in the combined validation cohort, VC1, VC2, and VC3 were 0.918 (95% CI 0.889-0.948), 0.923 (0.855-0.990), 0.918 (0.872-0.963), and 0.887 (0.813-0.960), respectively. Combination of TEPs and CA125 demonstrated an AUC of 0.922 (0.889-0.955) in the combined validation cohort; 0.955 (0.912-0.997) in VC1; 0.939 (0.901-0.977) in VC2; 0.917 (0.824-1.000) in VC3. For subgroup analysis, TEPs exhibited an AUC of 0.858, 0.859, and 0.920 to detect early-stage, borderline, non-epithelial diseases and 0.899 to discriminate ovarian cancer from endometriosis. TEPs had robustness, compatibility, and universality for preoperative diagnosis of ovarian cancer since it withstood validations in populations of different ethnicities, heterogeneous histological subtypes, and early-stage ovarian cancer. However, these observations warrant prospective validations in a larger population before clinical utilities.
Humans ; Female ; Blood Platelets/pathology* ; Biomarkers, Tumor/genetics* ; Ovarian Neoplasms/pathology* ; China

OBJECTIVES: To investigate the clinical treatment outcomes and the changes of the outcomes over time in extremely preterm twins in Guangdong Province, China. METHODS: A retrospective analysis was performed for 269 pairs of extremely preterm twins with a gestational age of <28 weeks who were admitted to the department of neonatology in 26 grade A tertiary hospitals in Guangdong Province from January 2008 to December 2017. According to the admission time, they were divided into two groups: 2008-2012 and 2013-2017. Besides, each pair of twins was divided into the heavier infant and the lighter infant subgroups according to birth weight. The perinatal data of mothers and hospitalization data of neonates were collected. The survival rate of twins and the incidence rate of complications were compared between the 2008-2012 and 2013-2017 groups. RESULTS: Compared with the 2008-2012 group, the 2013-2017 group (both the heavier infant and lighter infant subgroups) had lower incidence rates of severe asphyxia and smaller head circumference at birth (P<0.05). The mortality rates of both of the twins, the heavier infant of the twins, and the lighter infant of the twins were lower in the 2013-2017 group compared with the 2008-2012 group (P<0.05). Compared with the 2008-2012 group, the 2013-2017 group (both the heavier infant and lighter infant subgroups) had lower incidence rates of pulmonary hemorrhage, patent ductus arteriosus (PDA), periventricular-intraventricular hemorrhage (P-IVH), and neonatal respiratory distress syndrome (NRDS) and a higher incidence rate of bronchopulmonary dysplasia (P<0.05). CONCLUSIONS There is a significant increase in the survival rate over time in extremely preterm twins with a gestational age of <28 weeks in the 26 grade A tertiary hospitals in Guangdong Province. The incidences of severe asphyxia, pulmonary hemorrhage, PDA, P-IVH, and NRDS decrease in both the heavier and lighter infants of the twins, but the incidence of bronchopulmonary dysplasia increases. With the improvement of diagnosis and treatment, the multidisciplinary collaboration between different fields of fetal medicine including prenatal diagnosis, obstetrics, and neonatology is needed in the future to jointly develop management strategies for twin pregnancy.
Bronchopulmonary Dysplasia/epidemiology* ; Female ; Gestational Age ; Humans ; Infant ; Infant, Extremely Premature ; Infant, Newborn ; Pregnancy ; Respiratory Distress Syndrome, Newborn/epidemiology* ; Retrospective Studies ; Treatment Outcome

OBJECTIVE: To analyze the characteristics of gene mutation and overexpression in newly diagnosed multiple myeloma (NDMM) patients. METHODS: Bone marrow cells from 208 NDMM patients were collected and analyzed. The gene mutation of 28 genes and overexpression of 6 genes was detected by DNA sequencing. Chromosome structure abnormalities were detected by fluorescence in situ hybridization (FISH). RESULTS: Gene mutations were detected in 61 (29.33%) NDMM patients. Some mutations occurred in 5 or more cases, such as NRAS, PRDM1, FAM46C, MYC, CCND1, LTB, DIS3, KRAS, and CRBN. Overexpression of six genes (CCND1, CCND3, BCL-2, CCND2, FGFR3, and MYC) were detected in 83 (39.9%) patients, and cell cycle regulation gene was the most common. Single nucleotide polymorphisms (SNP) changes were detected in 169 (81.25%) patients, the TP53 P72R gene SNP (70.17%) was the most common. Abnormality in chromosome structure was correlated to gene overexpression. Compared to the patients with normal chromosome structure, patients with 14q32 deletion showed higher proportion of CCND1 overexpression. Similarly, patients with 13q14 deletion showed higher proportion of FGFR3 overexpression, whereas patients with 1q21 amplification showed higher proportion of CCND2, BCL-2 and FGFR3 overexpression. CONCLUSION There are multiple gene mutations and overexpression in NDMM. However, there is no dominated single mutation or overexpression of genes. The most common gene mutations are those in the RAS/MAPK pathway and the genes of cyclin family CCND are overexpression.
Chromosome Aberrations ; Humans ; In Situ Hybridization, Fluorescence ; Multiple Myeloma/genetics* ; Mutation

OBJECTIVE: To investigate the expression and clinical significance of serum protein ROCK2 in patients with chronic graft-versus-host disease (cGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS: The patients were divided into cGVHD group and control group (without cGVHD). The expression levels of serum protein ROCK2 were detected by ELISA in patients with or without cGVHD after allo-HSCT. RESULTS: The expression level of ROCK2 in serum of cGVHD patients was significantly higher than those in control group, moreover, the expression level of ROCK2 in severe cGVHD group was significant higher than that in moderate and mild cGVHD group (P<0.001). The expression level of ROCK2 was significantly decreased in the serum of cGVHD patients after treatment（P＜0.01）; the expression level of ROCK2 was significantly higher in the serum of cGVHD patients with lung as the target organ（P＜0.01）. The median survival time of patients with severe cGVHD were significantly shorter than that of patients with mild and moderate cGVHD（P＜0.05）. CONCLUSION ROCK2 shows certain reference value in the evaluation of severity and prognosis of cGVHD, and may be a new target for the treatment of cGVHD.
Blood Proteins ; Chronic Disease ; Graft vs Host Disease ; Hematopoietic Stem Cell Transplantation ; Humans ; Transplantation, Homologous ; rho-Associated Kinases

Objective:To investigate the hygienic condition and maintenance management of air conditioners in observation hotels， and give suggestions on reducing the risk of COVID-19 transmission from the daily use. Methods:This study selected 11 observation hotels chosen by government and 3 observation hotels chosen by large companies in Minhang District. The types and sanitary conditions of the air conditioning system were revealed through the daily supervision. Hotel staffs’ knowledge of air conditioning system and their mastery of how to use and maintain air conditioning system were surveyed through questionnaire. Results:Survey of air conditioning types showed that in 14 hotels， 12 were distributed air conditioning systems and 2 were semi-centralized air conditioning systems （including fresh air systems）. The investigation found that there was dust accumulation in the fresh air ducts in one hotel guest room， dust accumulation in the filter screen of fresh air intake in one hotel， and the sanitary problem of condensate water （without centralized discharge） in two hotels. All of 14 hotels had daily cleaning and disinfection records， but they were not perfect. The hotel health management personnel’ awareness rate of air conditioning was low， although they had a positive attitude towards the cleaning and disinfection of the air conditioning system. They could do the active entrusted testing， cleaning and disinfection of the air conditioning systems. Conclusion:The air conditioning systems of some hotels have hygiene problems， and hotel health management personnel are lack of knowledge of standard operation and maintenance of air conditioning systems. The air conditioning systems of observation hotel should be cleaned and disinfected before use. At the same time， it is necessary to strengthen the training of hotel health management personnel on the use and maintenance of air conditioning systems. So the transmission of the COVID-19 through air conditioning systems can be effectively prevented.