Objective　This study conducted a series of biochemical and genetic analyses on three strains of Comamonas kerstersii isolated, aiming to enhance understanding of the bacterium and provide a reference for preventing and controlling infections and diseases associated with it. Methods　The fecal samples of patients from three patients from September to October 2023 in Sanya People's Hospital were inoculated on Columbia blood agar plates, and after sufficient incubation, the target strains were isolated and purified using oxidase reagent. Subsequently, the target colonies were subjected to biochemical identification and antibiotic susceptibility testing using the VITEK2-Compact analysis system, while the VITEK MS mass spectrometer was used to identify the bacterial genus. Meanwhile, bacterial DNA was extracted for 16S rRNA sequencing and bacterial genome framework sequencing, with sequencing analysis performed using various bioinformatics analysis tools. Results　Three strains of oxidase-positive single colonies were cultured, and the mass spectrometer could not identify the specific strain. The drug susceptibility test showed that all three strains were sensitive to most drugs, but some were resistant to ciprofloxacin and Compound Sulfamethoxazole. 16S rRNA sequencing identified the bacteria to Comamonas genus, and bacterial genome framework sequencing analysis indicated that all bacteria were Comamonas kerstersii. Bioinformatics analysis showed that all three bacterial strains contained virulence factors related to oxidase-positive enteric pathogenic bacteria, including the flmH gene of Aeromonas, vctC gene and rmlB gene of Vibrio, and the flgG gene and flhC gene of Yersinia. Pathogen-host interaction (PHI) analysis results suggested pathogenic genes in the target bacterial genomes that may cause intestinal infection. Conclusions　Comamonas kerstersii cannot be identified by conventional mass spectrometer and requires sequencing for analysis. This bacterium is sensitive to most antibiotics, but individual strains are resistant to ciprofloxacin and Compound Sulfamethoxazole. Comamonas kerstersii is associated with infections, especially gastrointestinal infections, though its pathogenesis remains to be further studied.

AIM: To investigate the effect on growth and activity of telomerase in esophageal carcinoma cells by inhibiting ubiquitin-proteasome pathway(UPP). METHODS: The esophageal carcinoma cell strain Eca9706 was treated with MG-132 to inhibit its UPP specially. The effect of growth suppression on cells was evaluated with MTT assay, morphologic changes of cells were observed under microscope, cell cycle and apoptosis were detected by flow cytometry (FCM). DNA fragment analysis was used to confirm the presence of apoptosis. The activity of telomerase was detected. RESULTS: MG-132 had obvious inhibitory effect on the growth of Eca9706 cells in a dose and time-dependent manner. Obvious pathologic change of cells were observed under microscope, cells became round, small and exfoliating. The FCM analysis showed that the ratio of esophageal carcinoma cells of G_1 phase increased and a obviously apoptotic sub-G_1 peak was found. Agarose electrophoresis showed marked ladder. The activity of telomerase was obviously inhibited. CONCLUSIONS: MG-132 significantly inhibits the growth and the activity of telomerase of Eca 9706 cells. These findings indicate that inhibiting UPP is a new strategy for the treatment of esophageal carcinoma. [