Purpose: This study aimed to compare the diagnostic performance of cone-beam computed tomography (CBCT) and digital periapical radiography in detecting separated endodontic instruments (SI) according to their type and length infilled root canals of mandibular molars. Materials and Methods: Forty-two extracted mandibular molars were divided into 2 groups: control (without SI) and experimental (with SI). In the experimental group, SI fragments (2 or 3 mm) from 3 endodontic instruments (WaveOne Gold, Reciproc Blue, and ProTaper Next) were inserted into the mesiobuccal canals of 30 teeth. CBCT scans were obtained using the KAVO OP 3D PRO device, and digital periapical radiographs (orthoradial, distal, and mesial) were acquired using the Focus X-ray unit with the RVG 5200 solid sensor. Three experts independently evaluated all images for SI detection using a 5-point scale. Diagnostic performance metrics were calculated for each imaging method, andcomparisons were performed using 1-way analysis of variance with Tukey post-hoc tests. The significance level wasset at 5% (P = 0.05). Results: Digital periapical radiography demonstrated higher area under the receiver operating characteristic curve (Az), sensitivity, and specificity values than CBCT. ProTaper Next SI on CBCT scans showed the lowest Az values (0.58and 0.56 for 2- and 3-mm fragments, respectively). These values were significantly different from those of the other SIconditions (both CBCT and digital periapical radiography) (P<0.05). Conclusion Digital periapical radiography outperformed CBCT in detecting SI in filled root canals of mandibularmolars and should be the preferred modality. Furthermore, SI detectability varies with imaging modality, depending on instrument type and fragment length.

PURPOSE: The use of tolerogenic dendritic cells (TolDCs) to control exacerbated immune responses may be a prophylactic and therapeutic option for application in autoimmune and allergic conditions. The objective of this work was to evaluate the effects of TolDC administration in a mouse model of allergic airway inflammation caused by mite extract. METHODS: Mouse bone marrow-derived TolDCs were induced by incubation with granulocyte-macrophage colony-stimulating factor (GM-CSF) and dexamethasone, and then characterized by flow cytometry and cytokine production by enzyme-linked immunosorbent assay (ELISA). For the in vivo model of Blomia tropicalis-induced allergy, mice transplanted with antigen-pulsed TolDCs were sensitized intraperitoneally with B. tropicalis mite extract (BtE) adsorbed to aluminium hydroxide. After challenge by nasal administration of BtE, bronchoalveolar lavage fluid (BALF), lungs, spleen and serum were collected for analysis. RESULTS: Induction of TolDCs was efficiently achieved as shown by low expression of major histocompatibility complex (MHC) II, programmed death-ligand (PD-L) 2 and pro-inflammatory cytokine production, and up-regulation of interleukin (IL)-10, upon LPS stimulation in vitro. Transplantation of 1 or 2 doses of BtE-pulsed TolDCs reduced the number of inflammatory cells in BALF and lungs as well as mucus deposition. Moreover, compared to saline-injected controls, TolDC-treated mice showed lower serum levels of anti-BtE immunoglobulin E (IgE) antibodies as well as reduced Gata3 and IL-4 gene expression in the lungs and decreased IFN-γ levels in the supernatant of splenocyte cultures Transplantation of TolDCs increased the percentage of the regulatory T cells in the spleen and the lungs. CONCLUSIONS: Preventive treatment with TolDCs protects against dust mite-induced allergy in a mouse model, reinforcing the use of tolerogenic dendritic cells for the management of allergic conditions.
Administration, Intranasal ; Animals ; Antibodies ; Antigens, Dermatophagoides ; Asthma ; Bronchoalveolar Lavage Fluid ; Dendritic Cells* ; Dexamethasone ; Dust* ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Gene Expression ; Granulocyte-Macrophage Colony-Stimulating Factor ; Hypersensitivity ; Immunoglobulin E ; Immunoglobulins ; In Vitro Techniques ; Inflammation* ; Interleukin-4 ; Interleukins ; Lung ; Major Histocompatibility Complex ; Mice ; Mites* ; Mucus ; Spleen ; T-Lymphocytes, Regulatory ; Up-Regulation

This erratum is being published to correct the printing error on page 406 of the article. Corrections for author names are needed.