Background: Currently, colorectal cancer (CRC) ranks as the third most common cancer and the second leading cause of cancer-related mortality worldwide. CRC frequently metastasizes to the liver (50%), lungs (10–15%), peritoneum (4%), bones (10.7%–23.7%), brain (0.3%–6%), and spinal cord. Approximately 35% of CRC cases are diagnosed before distant metastasis, 36% upon lymph node involvement, and 23% after distant organ metastasis. Although several studies have established primary tumor models in mice in our country, there are limited studies on experimental lung metastasis models, prompting the need for this research. Aim: To establish and evaluate a lung metastasis model of colorectal cancer in C57BL/6J mice using the MC38 cell line. Materials and Methods: This study was conducted at the Institute of Biomedical Sciences, Mongolian National University of Medical Sciences. Approval was obtained from the Ethics Review Board of the Mongolian National University of Medical Sciences (2023/3-09) and all laboratory safety regulations and protocols were strictly followed. Male C57BL/6J mice bred at the Experimental Animal Center of Mongolian National University of Medical Sciences were used. MC38 murine colorectal carcinoma cells were cultured and injected intravenously (via the tail vein) at a concentration of 0.25×10⁶ cells per mouse (n=12) to induce lung metastasis. Histological analysis was subsequently performed. Results: Histological examination revealed significant alterations in lung tissue architecture, characterized by areas of dense infiltration by pleomorphic, hyperchromatic cells, disrupting the normal alveolar structure. No histological abnormalities were observed in other organs. Conclusion Intravenous injection of MC38 colorectal adenocarcinoma cells into the tail vein of C57BL/6J mice successfully induced lung metastases, characterized by hyperchromatic, pleomorphic cell infiltrates forming glandular structures within the lung parenchyma.

Background: Atopic dermatitis (AD) is a common chronic and relapsing inflammatory skin disease in young children, clinically characterized by pruritus and skin rashes. Epidemiological studies have reported that AD affects 5–30% of children and 1-10% of adults worldwide. In recent years, the use of mineral waters for the treatment of chronic skin conditions has become increasingly popular globally. Although mineral waters, particularly from Shargaljuut springs, have been traditionally used in Mongolia for various dermatological conditions, no studies have been conducted on their use in atopic dermatitis, providing the rationale for this study Aim: To evaluate the result of Shargaljuut mineral spray on disease severity, pruritus intensity, and selected physiological parameters of the skin in children with atopic dermatitis. Materials and Methods: This clinical trial study with a placebo-controlled group included children aged 2-16 years with AD. The participants were treated with Shargaljuut spray and the clinical assessment was conducted before and after the treatment, evaluating severity using SCORAD (Severity Scoring of Atopic Dermatitis), itching intensity using VAS (Visual Analog Scale), skin hydration (SCH) and transepidermal water loss (TEWL). Results: Among all participants, 81.7% (n=49) had mild atopic dermatitis based on SCORAD scores, and 18.3% (n=11) had moderate severity. According to the VAS scores, 40% (n=24) of the participants reported mild pruritus. Skin hydration was found to be severely decreased in 96.7% (n=58) of the cases, while TEWL values were within normal limits in 76.7% (n=46). Statistically significant differences were observed between the experimental and control groups at day 30 in terms of disease severity and pruritus intensity, as well as in certain skin physiological parameters (p=0.001, p=0.002). Within the experimental group, statistically significant differences were also detected in disease severity (p=0.001), pruritus intensity (p=0.002), skin hydration (p=0.002), and TEWL (p=0.001) between baseline, day 15, and day 30. A correlation was found between transepidermal water loss (TEWL) and skin hydration (r=0.566, p=0.0001). Conclusions 1. Among the children with atopic dermatitis (AD) included in the study, 81.7% (n=49) had a mild severity score according to SCORAD, 40% (n=24) had mild itching intensity based on the VAS assessment, 96.7% (n=58) had excessively dry skin, and 76.7% (n=46) showed good transepidermal water loss (TEWL) levels. 2. In the experimental group, comparisons of disease severity, itching intensity, skin hydration, and TEWL levels before and after the use of the Shargaljuut spring water spray revealed that skin hydration increased, TEWL decreased, and both disease severity and itching intensity were reduced following treatment

Background: Insomnia is one of the most prevalent neurological disorders in the United States, affecting up to half of primary care patients and often necessitating psycho-behavioral interventions. Acupuncture, a key component of traditional Chinese and Mongolian medicine, has been increasingly studied as a treatment for insomnia in recent years. Aim: Evaluating the effectiveness of acupuncture for insomnia Materials and Methods: The study was conducted using a randomized controlled clinical trial design. 148 participants, aged 18-65 years, with a diagnosis of Nonorganic Insomnia (F51.0) according to the ICD-10, and Pittsburgh Sleep Quality Index (PSQI) >7, were divided into the transverse acupuncture, conventional acupuncture, and the control group. The results were analyzed using a Cardiopulmonary Coupling - CPC machine before and after treatment in the 3 groups to assess 1. Total sleep (hours), 2. Deep sleep (hours), 3. Light sleep (hours), 4. REM (hours), 5. Wake time (minutes), 6. Time to first fall asleep (minutes), 7. Sleep rate (%), 8. Number of apneas, and sleep quality was assessed using the Insomnia Severity Index (ISI) and Pittsburgh Sleep Quality Index (PSQI) questionnaires. The research approval was reviewed and granted by the Research Ethics Review Committee of the Mongolian National University of Medical Sciences on January 19, 2024 (Approval No. 24/19/01). Results: The mean age of participants was 51.5±10.8 years, with 79.7% (n=118) being female and 20.3% (n=30) male. In the transverse acupuncture group, post-treatment results demonstrated a 53.8% increase in total sleep time, a 102.8% increase in deep sleep time, a 19.8% increase in light sleep time, a 36.1% increase in REM sleep time, and a 22.1% improvement in sleep normalization rate. The mean differences between pre- and post-treatment scores were statistically significant based on a one-sample t-test. In contrast, no statistically significant improvements were observed in the control group, except for sleep quality. Conclusion Both transverse acupuncture and conventional acupuncture significantly improved sleep quality indicators following treatment.

Background: Vaginal candidiasis is a highly prevalent infectious disease among women caused by a wide range of pathogenic organisms. Although it can be treated with standard pharmacological methods, it has detrimental effects on women’s reproductive health. The absence of research to date on the anti-vaginal inflammatory effects of Jirug-6 suppositories - a traditional formulation derived from ancient medicinal recipes and long-used in traditional mongolian medicine has provided the rationale for conducting this study. Aim: Study of the effects of Jirug-6 suppositories on the vaginal inflammatory lesion model induced by Candida albicans. Materials and Methods: To establish the vaginal inflammatory lesion model in experimental animals, the inoculum was prepared according to the McFarland standard method, and 10 µL of the inoculum was intravaginally administered daily for 4 consecutive days. Fifty-six mice with induced vaginal candidiasis were divided into 7 groups. On treatment days 1, 3, 5, 7, and 10, the vaginal cavity of the mice was washed with 100 µL of phosphate buffer. From the lavage fluid, 20 µL was collected, mixed with 480 µL of phosphate buffer, and thoroughly homogenized. Using a 20 µL micropipette, three aliquots were inoculated onto Sabouraud Dextrose Agar (SDA) medium and incubated at 37°C for 48 hours. Colonies were counted, and at the end of the experiment, serum was isolated from blood samples, and IFN-γ and IL-10 levels in the samples were quantified using ELISA. Histopathological analysis was performed on vaginal tissues from the experimental animals. Results: In the experimental animals, Jirug-6 suppositories 0.4 mg/kg dose exhibited the mildest clinical signs of hyperemia, swelling, hemorrhage, infiltration, or fluid loss into soft tissues in the vaginal and cervical regions (p<0.01). For cervical inflammation, Jirug-6 suppositories 0.4 mg/kg demonstrated a strong anti-inflammatory effect with a score of 1.99±0.78, which was comparable to the positive control group (p<0.01). In serum, the concentrations of IFN-γ and IL-10 in Jirug-6 suppositories 0.4 mg/kg were determined to be 8.18±0.61 and 7.91±1.30, respectively, showing reduced levels similar to those of the positive control group (p<0.01). Conclusions 1. Jirug-6 suppositories reduce inflammatory symptoms and suppresses fungal dissemination during the vaginal lesion model induced by C. albicans. 2. At a Jirug-6 suppositories 0.4 mg/kg demonstrates anti-inflammatory effects by increasing IFN-γ concentration and decreasing IL-10 concentration in the C. albicans-induced vaginal lesion model. Furthermore, it enhances immunity and reduces swelling during inflammation, exhibiting positive therapeutic effects against clinical symptoms.

Background: The most common clinical manifestation of HCV infection, which includes both hepatic and extrahepatic manifestations, is mixed cryoglobulinemia, which is characterized by the precipitation of certain proteins in the blood at temperatures below 37°C (in vitro), aggregation, and deposition in the walls of small and medium-sized vessels, causing vasculitis, which is clinically manifested by a triad of joint pain, fatigue, and rash on the soles of the feet. Cryoglobulinemia is commonly diagnosed in people with HCV infection, with a prevalence ranging from 10% to 70%. Vasculitis that occurs when cryoglobulinemia is detected mainly affects the small vessels of the skin, kidneys, and peripheral nerves, causing complications in other organ systems. Aim : To determine the prevalence of cryoglobulinemia in people with HCV infection, study it in relation to the stage of liver fibrosis, and determine its clinical relevance. Materials and Methods : 200 chronic HCV infected individuals were included in the study according to the inclusion and exclusion criteria. After obtaining informed consent from each participant, a questionnaire was used to collect information, perform physical measurements, and collect peripheral blood samples. Complete blood count and biochemical tests (liver and kidney function) were performed. The degree of liver fibrosis was assessed non-invasively (APRI, FIB4). The glomerular filtration rate was calculated electronically using the MDRD GFR Equation. Skin examination was performed to assess the presence of rash, ulcers, and scarring on the shins and ankles of cryoglobulinemia. To determine cryoglobulinemia, 8 ml of blood was collected in a tube without anticoagulant, and the sample was kept motionless for 1 hour at room temperature until clotting was complete. After centrifugation, the samples were separated and stored in a refrigerator at +4°C for 7 days, and then at room temperature for 30 minutes, the precipitate was detected. Results : A total of 200 people participated in the study, of which 71 were men (35.5%), the average age was 53.39±13.0. Cryoglobulinemia protein precipitates were determined in a total of 148 people, of which 50 were men (33.8%), the average age was 52.95±13.0. Cryoglobulinemia protein precipitates were detected in 89 people, or 60.1% of the study participants. Of the total study participants, 176 (88.0%) had chronic hepatitis C (CHC). Of these, 57 people had CHC with cryoglobulinemia. Comparing laboratory parameters, the mean GGT level in the cryoglobulinemia group was statistically significantly higher than in the non-cryoglobulinemia group (p=0.039). However, when laboratory parameters were grouped by increasing or decreasing, AST and ALT levels were significantly higher in the cryoglobulinemia group, indicating more hepatocellular damage (p<0.000). Increased creatinine levels may be associated with the risk of renal dysfunction. The FIB-4 index and APRI index showed a more severe degree of fibrosis in the cryoglobulinemia group (p<0.005; p<0.000). Univariate logistic regression analysis showed that age was associated with the occurrence of cryoglobulinemia (OR=2.48; 95% CI:1.31–4.70; p=0.005). Platelet count had a statistically significant positive effect in multivariate analysis (OR=14.38; 95% CI:1.26–163.89; p=0.032). Conclusion The prevalence of cryoglobulinemia among HCV-infected patients was 60.1%, and older age and decreased platelet count among infected individuals were associated with the occurrence of cryoglobulinemia.

Background: The prevalence of Helicobacter pylori infection varies by geographic location, age, race, and socioeconom ic status. Approximately 30% of the population in developed countries, about 70% of the population in developing countries, and around 50% of the global population are infected with Helicobacter pylori. While the prevalence is generally high in developing countries, it is lower in developed countries. Some studies conducted in our country have shown that the prevalence of this infection is high among students and young people. Therefore, we aimed to study the prevalence of Helicobacter pylori infection among students. Aim: “We will study the prevalence of Helicobacter pylori infection among the students of the School of Nursing at MNUMS and compare it with their social, demographic information and clinical symptoms. Materials and Methods: “The study was conducted using a cross-sectional quantitative research design and a random sampling method, involving a total of 62 first-year students from the School of Nursing between November 2023 and March 2024. A questionnaire including social, demographic information and clinical symptoms was used to assess the prevalence of Helicobacter pylori infection. Blood samples from the participants were tested for antibodies at the Onch Clinical Laboratory. Results: Among the 62 students who participated in our study, a majority 79.0% tested positive for Helicobacter pylori infection. When examining whether this infection was associated with age, gender, or specialty, it was found in 82.1% of those aged 18-20, 81.4% of females, and 87.5% of those studying in specialized programs. However, no statistically significant association was observed when compared to the non-infected group (p>0.05). In terms of clinical symptoms, stomach and epigastric pain was reported in 84.1%, unpleasant taste or bad breath in 88.6%, and occasional vomiting in 83.3% of those with Helicobacter pylori infection all of which were more prevalent in the infected group and showed statistically significant associations (p<0.05). Conclusions Among the 62 participants in the study, the majority, 49 individuals (79.0%), tested positive for Helicobacter pylori infection. The group with Helicobacter pylori infection exhibited a higher prevalence of clinical symptoms, which showed a statistically significant association (p<0.05).

Background: The morphological characteristics of the alveolar bone thickness between the maxillary sinus and root ca nal tips of the premolars and molars is important for dental implant and orthodontic treatment. The hypotheses formulated were that there would be differences in the morphological characteristics of the alveolar bone at various tooth positions in the posterior maxilla and that age and sex would not influence the findings. This study may provide useful information for dental implant and mini-implant treatment, so as to help lead to successful treatment outcomes. Aim: To evaluate the vertical relationship between the maxillary sinus and molar teeth using cone beam computed tomography (CBCT). Materials and Methods: The study design was retrospective study. We selected 30 CBCT images that were taken in the Central Dental Hospital, Mongolian National University Medical Sciences (MNUMS), between 2021 and 2023. We collected all images according to the inclusion and exclusion criteria. We used Full CBCT (16cm*8cm) images using the target sampling method. The all CBCT images (85kW, 7mA) were obtained with HDX, WILL (DENTRI, Seoul, Korea) using OnDemand3D (CyberMed. Seoul. Korea). The vertical relationship between the maxillary sinus and the maxillary molars was classified into 5 categories according to Kwak’s classification. The study was approved by the Research Ethics Committee of MNUMS. Statistical analyses were done by IBM SPSS version 27 software. Results: A total of 202 maxillary premolars and molars were examined using CBCT images, and were taken in 30 patients. The patient group consisted of 12 men and 18 women with an average age of 26.87 years (range, 16–42 years). In the maxillary first premolars: Class I was 77.4%, Class II was 13.2%, Class V was 9.4%, Classes III and IV were not observed. In the maxillary second premolars: Class I was 44.8%, Class V was 37.9%, Class II was 17.2%. In the maxillary first molars: Class V was 62.3%, Class I was 24.4%, Class II was 6.7%, Class III was 4.4%, Class IV was 2.2%. In the maxillary second molars: Class V was 63.1%, Class I was 23.9%, Class II was 6.5%, Class III was 6.5%. When examining the distance from the apex of the root of the molars to the floor of the maxillary sinus, the distance between the root of the buccal side of the second molar was the smallest, 0.67±1.36mm, and the distance between the root of the first premolar was the furthest, 5.14±4.32mm (p<0.01). Conclusion Among the maxillary posterior teeth, the second molar was positioned closest to the floor of the maxillary sinus, whereas the first premolar was located at the greatest distance. Regarding the maxillary molars, Type V characterized by root protrusion into the floor of the maxillary sinus was the most frequently observed configuration. Conversely, for the premolars, Type I defined by a distinct separation from the sinus floor was the predominant anatomical relationship.

Background: Cardiovascular diseases (CVDs) is a third leading cause of non-communicable diseases and a leading cause of mortality in Mongolia. Among these, acute myocardial infarction (AMI), a complication of coronary artery disease (CAD), is a leading cause of cardiovascular mortality. In patients with AMI, both epicardial artery blood flow, observed through coronary angiography, and coronary microcirculatory dysfunction (CMD) play critical roles in the disease’s pathophysiology. Recent advancements have introduced artificial intelligence (AI)-based softwares which are capable of analyzing coronary blood flow from coronary angiography images. The AI based softwares can assess not only epicardial blood flow but also CMD in recent studies. In this review, we discussed the role of CMD in pathophysiology of AMI, diagnostic approaches, the application of AI-based technologies, their clinical significance, and future directions. Aim: This review aims to explore the pathophysiological relationship of coronary microcirculatory dysfunction (CMD) in the context of acute myocardial infarction (AMI), diagnostic methodologies, the application of artificial intelligence (AI)-based technologies, and their clinical significance. Hypotheses: 1. AI-based software allows for highly accurate assessment of coronary microcirculation during AMI. 2. CMD during AMI is associated with structural and functional changes in the heart. 3. CMD during AMI is related to disease prognosis and patient outcomes. Methodology:: This narrative review was compiled using a systematic and logical approach, based on publicly available recent clinical and biomedical research literature addressing the above hypotheses. Conclusion In recent years, AI-based image processing software has been developed to analyze angiographic images obtained during percutaneous coronary intervention (PCI) and treatment procedures. These tools show significant promise for early detection and improved diagnosis of CMD during AMI, as well as for better assessment of short- and long-term patient risk.

Backround Peutz–Jeghers (PJ) syndrome is a rare autosomal dominant disorder characterized by a mucocutaneous pigmentationon on oral mucosa and multiple hamartomatous polyps located in the digestive tract except esophagus. PJ syndrome can be diagnosed in early childhood by a characteristic pigmentation and family history of polyposis. However, it is often diagnosed first as a polyp in the small intestine that causes obstruction and intussusception and is often treated with a bowel resection. If diagnosed in young childhood, an effective non-invasive method is to resect the polyps by tying off the blood supply to the polyps, that is the method named ischemic polypectomy, before they grow to the point of obstruction using a endoscopy. PJ syndrome is rare in Mongolia, but in severe cases, small intestine polyps are treated only surgically. Double-balloon-endoscopy (DBE) has been performed at the Mongolian- Japanese Hospital since 2023, making it possible to diagnose and treat the syndrome endoscopically. Our patient, a 15-year-old boy, had a mucocutaneous pigmentation that had been previously undiagnosed and was first diagnosed with intussusception at the age of 13. He had undergone 4 endoscopic procedures for upper and lower gastrointestinal polyps at the National Center for Maternal and Child Health successfully. In our hospital, we found endoscopically multiple hamartomatous polyps of various sizes between 1-3 cm, and a 3 mm diameter tumor that filled 3/4 of the intestinal lumen was treated by ischemic polypectomy. After the procedure, there were no early or late complications related to the procedure. The child's condition improved, the main complaints subsided, and he continues his daily life normally. However, follow-up DBE is required.

Background: In traditional medicine, Jur Ur-4 Tang, which is widely used for the treatment of diabetes, has been technologically processed and transformed into a modern pharmaceutical form as soluble granules. Therefore, it is essential to evaluate the pharmacological effects of the soluble granules derived from Jur Ur-4 Tang. Aim: To study the effect of granules extracted from Jur ur-4 Tang in a model of type 2 diabetes mellitus in experimental rats. : Materials and Methods : T2DM in rats was induced by a high-fat diet and a low-dose streptozotocin (STZ 35 mg/kg). Then, oral gavage administration of three different doses of Granule (450 mg/kg, 900 mg/kg) was given to T2DM rats. Experimental results showed that Granule dramatically reduced the levels of fasting blood glucose, fasting blood insulin, and GLUT4. Results:: In the glucose tolerance test, granules at doses of 450 and 900 mg/ kg significantly reduced blood glucose levels at 60 and 120 minutes (p < 0.01). Additionally, the soluble granules at all tested doses significantly decreased blood insulin levels and increased GLUT4 expression (p<0.01). Conclusion The granules lower blood glucose levels.