Objectives To investigate interleukin 36γ (IL-36γ) expression, and analyze the influence of IL-36γ to CD8⁺ T cell activity in chronic obstructive pulmonary diseases (COPD) patients with secondary fungal pneumonia. Methods Peripheral blood was collected from 47 COPD patients, 39 COPD patients with secondary fungal pneumonia, and 20 controls. Bronchial alveolar lavage fluid (BALF) was isolated from 27 COPD patients with secondary fungal pneumonia. CD8⁺ T cells were purified. The levels of four IL-36 isoforms in plasma and BALF were measured by enzyme linked immunosorbent assay (ELISA). CD8⁺ T cells were stimulated with recombinant human IL-36γ. The levels of interferon γ(IFN-γ), tumor necrosis factor α(TNF-α), perforin and granzyme B in the cultured supernatants were measured by ELISA. Recombinant human IL-36γ-stimulated CD8⁺ T cells were co-cultured with NCI-H1882 cells in either direct cell-to-cell contact or Transwell^TM manner. The levels of IFN-γ, TNF-α, and lactate dehydrogenase in the cultured supernatants were assessed. The percentage of target cell death was calculated. Results Plasma IL-36α, IL-36β, and IL-36γ levels were significantly elevated in both COPD group and COPD with secondary fungal pneumonia group compared with those in control group. However, only plasma IL-36γ level was higher in COPD with secondary fungal pneumonia group than that in COPD group [(200.11±99.95)pg/mL vs (53.03±87.18)pg/mL, P=0.023]. There was no remarkable difference in plasma IL-36 receptor antagonist level among three groups. IL-36γ level in BALF from infectious site was higher than that from non-infectious site in COPD with secondary fungal pneumonia group [(305.82±59.60)pg/mL vs (251.93±76.01)pg/mL, P=0.011]. IL-36γ stimulation enhanced IFN-γ, TNF-α, perforin and granzyme B secreted by CD8⁺ T cells. When IL-36γ-stimulated CD8⁺ T cells were directly mixed with NCI-H1882 cells for co-culture, the percentage of cell death was increased [(16.06±3.67)% vs (11.47±2.36)%, P=0.002]. When using Transwell^TM plate for non-contact co-culture, IL-36γ-stimulated CD8⁺ T cell-mediated death of NCI-H1882 cells showed no significant difference compared to that without stimulation [(4.77±0.78)% vs (4.99±0.92)%, P=0.554]. Conclusion IL-36γ level in plasma and infectious site is elevated in COPD patients with secondary fungal pneumonia, which enhances the cytotoxicity of CD8⁺ T cells in peripheral blood and infectious microenviroment.
Humans ; Pulmonary Disease, Chronic Obstructive/complications* ; CD8-Positive T-Lymphocytes/metabolism* ; Male ; Female ; Aged ; Middle Aged ; Interferon-gamma/metabolism* ; Interleukin-1/metabolism* ; Tumor Necrosis Factor-alpha/metabolism* ; Lung Diseases, Fungal/complications* ; Bronchoalveolar Lavage Fluid/chemistry* ; Perforin/metabolism* ; Pneumonia/immunology* ; Granzymes/metabolism*

Percutaneous coronary intervention (PCI) has become an important means of revascularization for patients with coronary heart disease (CHD) . Exercise therapy is the core content of cardiac rehabilitation for patients after PCI. Baduanjin, one of the traditional exercise methods of traditional Chinese medicine that spreads widely and enjoys obvious fitness effects, shows a positive impact on the rehabilitation of patients after PCI. This article reviews the effects of Baduanjin on the cardiopulmonary function, psychological status, quality of life, physical and chemical indicators, and adverse cardiovascular events in patients after PCI from different perspectives, in order to provide a reference for the practice and research of Baduanjin in the rehabilitation of patients after PCI.

Objective:To understand the current situation and influencing factors of exercise intention among patients after percutaneous coronary artery stent implantation (CASI) , so as to promote patients to actively participate in exercise.Methods:Using the convenient sampling method, 165 patients with CASI who revisited in the cardiology department of a Class Ⅲ Grade A hospital in Shandong Province from May to August 2020 were selected as the research objects. General information questionnaire, Exercise Intention Scale, Exercise Self-efficacy Scale and Exercise Social Support Scale were used for investigation.Results:In this study, 165 questionnaires were distributed and 154 valid questionnaires were returned, with an effective recovery rate of 93.33%. The score of Exercise Intention Scale for 154 patients after CASI was (12.46±4.05) . The results of multiple linear regression analysis showed that age, moderate-intensity activity in the past week, classification of cardiac function, exercise self-efficacy and exercise social support were influencing factors of exercise intention among patients after CASI ( P<0.05) , which explained 47.6% of the total variation. Conclusions:The exercise intention of patients after CASI needs to be further improved and there are many factors that affect exercise intention of patients. Family members of patients and medical staff should provide more exercise support to improve their exercise self-efficacy.