Objective To investigate the correlation between water-soluble fibre and insulin resistance(IR)through regulating intestinal flora.Methods 24 male SD rats were randomly divided into normal control(NC)group,high-fat diet(HFD)group,and mixed 10%water-soluble fibre(F-HFD)group,with eight rats in each group.Body weight,blood glucose,serum branch chain amino acids(BCAAs)and FIns were measured.Glucose tolerance test and insulin tolerance test were performed.ISI and assessment-insulin resistance(HOMA-IR)was calculated.16SrDNA gene amplification and sequencing were performed to determine the composition of gut microorganisms in the intestinal contents of the rats.Results The FPG,HOMA-IR,and BCAAs were sequentially increased.FIns was higher in HFD group than in NC and F-HFD groups(P<0.05),ISI was lower in HFD group than in NC and F-HFD groups(P<0.05).The relative abundance of Prevotella and Bacteroides in feces was significantly higher(P<0.05)and Roseburia was significantly lower(P<0.05)in the HFD group compared with the NC group.The relative abundance of Prevotella was decreased in the F-HFD group compared with the HFD group(P<0.05).Correlation analysis showed that BCAAs were positively correlated with IR,glucose tolerance and Prevotella(P<0.05).Conclusions Water-soluble fibre may reduce the production of BCAAs by down-regulating the abundance of intestinal flora Prevotella in rats on a high-fat diet,leading to IR reduction.

Objective To investigate the correlation between water-soluble fibre and insulin resistance(IR)through regulating intestinal flora.Methods 24 male SD rats were randomly divided into normal control(NC)group,high-fat diet(HFD)group,and mixed 10%water-soluble fibre(F-HFD)group,with eight rats in each group.Body weight,blood glucose,serum branch chain amino acids(BCAAs)and FIns were measured.Glucose tolerance test and insulin tolerance test were performed.ISI and assessment-insulin resistance(HOMA-IR)was calculated.16SrDNA gene amplification and sequencing were performed to determine the composition of gut microorganisms in the intestinal contents of the rats.Results The FPG,HOMA-IR,and BCAAs were sequentially increased.FIns was higher in HFD group than in NC and F-HFD groups(P<0.05),ISI was lower in HFD group than in NC and F-HFD groups(P<0.05).The relative abundance of Prevotella and Bacteroides in feces was significantly higher(P<0.05)and Roseburia was significantly lower(P<0.05)in the HFD group compared with the NC group.The relative abundance of Prevotella was decreased in the F-HFD group compared with the HFD group(P<0.05).Correlation analysis showed that BCAAs were positively correlated with IR,glucose tolerance and Prevotella(P<0.05).Conclusions Water-soluble fibre may reduce the production of BCAAs by down-regulating the abundance of intestinal flora Prevotella in rats on a high-fat diet,leading to IR reduction.

Objective To investigate the causes of infertility and its pathological mechanism in female SD rats with spontaneous dwarfism(short stature rat,SSR).Methods Adult wildtype and SSR female SD rats were used in this study.A vaginal smear was used to observe changes in the motile cycle.Ovulation promotion was compared using the simultaneous estrus supernumerary ovulation method.Ovarian and uterine weight and body weight,and ovarian and uterine indices were measured.AMH,E2,FSH,LH,and FSH/LH levels in serum were measured.Transcriptome sequencing of ovarian tissues was performed to analyze gene expression differences.Results No abnormalities were observed in the estrous cycle of SSR female rats.The body weight of SSR female rats was significantly lower than that of wildtype rats,and their ovarian and uterine indices were significantly higher than that of wildtype rats.The mean number of ovulations was significantly higher in wildtype rats than in SSR female infertile rats(P<0.001).Serum AMH(P<0.01)and E2(P<0.05)levels were significantly higher in wildtype rats than in SSR female infertile rats,and serum levels of FSH,LH,and FSH/LH(P<0.05)were significantly lower in SSR infertile females than in SSR infertile rats,while PROG showed no significant difference.Transcriptome sequencing yielded 250 differentially expressed genes,including 190 upregulated and 60 downregulated genes.p53 signaling pathway and cytokine-cytokine receptor interaction.The MCC,MNC,EPC,and degree calculations of the CytoHubba plug-in were used to screen the top 10 significant nodes.The intersection was used to finally obtain nine hub genes,namely Cxcl1,Cxcl2,IL1a,IL1b,Cd80,Mmp13,Mmp8,Fgf3,and Ptgs2.Conclusions Infertility in SSR female rats may be related to a decreased ovarian reserve function and poor ovarian response.Cxcl1,Cxcl2,IL1a,IL1b,Cd80,Mmp13,Mmp8,Fgf3,and Ptgs2 were associated with infertility,laying a theoretical foundation to further explore infertility mechanisms.