Objective:To investigate the hepatoprotective effect of N-acetylcysteine(NAC)on rats with liver injury induced by cisplatin and its effect on intestinal flora and the expression of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and nuclear factor-kappa B(NF-κB).Methods:Male Sprague-Dawley rats were randomly divided into control group(CG),cisplatin group(CP),and NAC group.The rats in the NAC group were given NAC 15 mg/kg by gavage for 8 consecutive days.At half an hour after intragastric administration on the fifth day,all rats except those in the NC group were given intraperitoneal injection of 8 mg/kg cisplatin to induce acute liver injury.An automatic biochemical analyzer was used to measure the content of aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP),and total bilirubin(TBIL);liver index was calculated for the rats;Western blot was used to measure the relative expression levels of NF-κB,IL-6,and TNF-α in liver tissue;the 16S rDNA technique was used to measure and analyze the amplification information of the V3-V4 regions of each sample.Results:Compared with the NC group,the CP group had significant increases in the content of AST,ALT,ALP,and TBIL,while NAC reversed the abnormal liver function caused by cisplatin.Compared with the NC group,the CP group had a sig-nificant increase in liver index(P=0.000),while the NAC group had a significant reduction in liver index compared with the CP group(P=0.007).Compared with the NC group,the CP group had signifi-cant increases in the expression levels of IL-6,TNF-α,and NF-κB,while the NAC group showed reductions in the expression of these genes,with significant differences in the expression of IL-6 and TNF-α(P=0.006 and 0.000).Compared with the NC group,the CP group had a significant increase in the α-diversity index of intesti-nal flora,while compared with the CP group,the NAC group tended to have a reduction in the α-diversity index of intestinal flora.Com-pared with the CP group at the phylum level,the NAC group had an increase in the abundance of Actinobacteria and a reduction in the abundance of Firmicutes.Compared with the CP group at the genus level,the NAC group had a reduction in the abundance of Rumino-coccaceae and increases in the abundance of Bifidobacterium and Allobaculum.Conclusion:NAC can alleviate acute liver injury caused by cisplatin,possibly by downregulating the expression of IL-6,TNF-α,and NF-κB and regulating the abundance and diver-sity of intestinal flora.

Objective To analyzes the Chinese and English literature of anti-tumor of licorice based on CiteSpace knowledge graph,and explores the research status,research hotspots and trends.Methods CiteSpace 6.2.R4 software was used to visually analyze the Chinese and English literature on licorice anti-tumor from January 2002 to September 2023 included in CNKI and Web of Science,and visually analyzed the research topics such as the number of publications,authors,institutions,journals,funds,and keywords.Results A total of 397 Chinese articles and 172 English articles were included after screening.The analysis of the authors of Chinese and English literature shows that Zheng Qiusheng and Jung are the authors with the largest number of anti-tumor articles in Chinese and English respectively,while Beijing University of traditional Chinese Medicine and Hallym University have the largest number of articles in Chinese and English respectively,and there is relatively more cooperation among research institutions in English literature.Chinese and English keyword analysis shows that the anti-tumor research of Glycyrrhiza uralensis mainly focuses on breast cancer,licorice and its active components,molecular mechanism and so on.Cluster analysis shows that research has focused on the active ingredients and clinical applications of licorice;Traditional Chinese Medicine treatment theory and commonly used compound medicines;Experimental research and mechanism of action of licorice on anti-tumor effects;Research on the mechanism of licorice in preventing and treating nephrotoxicity and other toxic side effects caused by chemotherapy drugs.Conclusion The study of anti-tumor mechanism of licorice and the combination of licorice and natural products may be the focus of future research.

Guided by the demands of society,we propose a new professional training objectives of medical laboratory technology,which is to cultivate comprehensive talents covering the entire industrial chain of medical laboratory technology with profound foundation,broad caliber,high quality and outstanding ability.According to the training objective,we build up 4321 talents training system and try to carry out preliminary practice and exploration on talent cultivation model from the following aspects,such as the construction of curriculum system,the reform of the teaching contents and methods,training of students' professional skills and entrepreneurial innovation spirit.