The Expert Consensus on Clinical Application of Qinbaohong Zhike Oral Liquid in Treatment of Acute Bronchitis and Acute Attack of Chronic Bronchitis （GS/CACM 337-2023） was released by the China Association of Chinese Medicine on December 13^th， 2023. This expert consensus was developed by experts in methodology， pharmacy， and Chinese medicine in strict accordance with the development requirements of the China Association of Chinese Medicine （CACM） and based on the latest medical evidence and the clinical medication experience of well-known experts in the fields of respiratory medicine （pulmonary diseases） and pediatrics. This expert consensus defines the application of Qinbaohong Zhike oral liquid in the treatment of cough and excessive sputum caused by phlegm-heat obstructing lung， acute bronchitis， and acute attack of chronic bronchitis from the aspects of applicable populations， efficacy evaluation， usage， dosage， drug combination， and safety. It is expected to guide the rational drug use in medical and health institutions， give full play to the unique value of Qinbaohong Zhike oral liquid， and vigorously promote the inheritance and innovation of Chinese patent medicines.

Pancreatic cancer has emerged as one of the most challenging malignancies worldwide,with its high resistance to chemotherapy being the primary cause of treatment failure.Therefore,enhancing the chemosensitivity of pancreatic cancer has become a major focus of current research.In this study,we in-vestigated how Bufotaline,a bufadienolide extracted from the traditional Chinese medicine toad venom,exhibits its antitumor activity.Specifically,we explored the potential of Bufotaline to enhance the chemo-sensitivity of pancreatic cancer cells to Adriamycin and elucidated its underlying molecular mechanisms.Using CCK-8 and colony formation assays,we demonstrated that Bufotaline enhances the inhibitory effect of Adriamycin on the survival of pancreatic cancer cell lines Patu-8988T,Aspc-1,and Patu-8988S.No-tably,Bufotaline treatment reduced the IC50 of Adriamycin in drug-resistant pancreatic cancer cells to lev-els comparable to those in non-resistant cells.Results from Western blot,immunofluorescence,comet as-say,and TUNEL assays revealed that Bufotaline promotes Adriamycin-induced DNA damage in pancreatic cancer cells.RNA-seq analysis of Patu-8988T cells treated with Adriamycin alone or in combination with Bufotaline showed significant changes in gene expression,and qRT-PCR analysis further confirmed that Bu-fotaline downregulates the expression of DNA damage repair proteins NBS1 and RAD50.Moreover,Western blot analysis revealed that Bufotaline reduces the levels of DNA damage response repair proteins,and Im-munofluorescence experiments indicated that Bufotaline inhibits the activation of the ATM/CHK2 signaling pathway.Finally,in a subcutaneous xenograft mouse model,the combination of Adriamycin and Bufotaline treatment significantly suppressed pancreatic cancer cell growth.In conclusion,Bufotaline enhances Adria-mycin-induced chemosensitivity in pancreatic cancer cells;the combination of Adriamycin and Bufotaline downregulates the expression of DNA damage response repair proteins NBS1 and RAD50,and inhibits the ATM/CHK2-mediated DDR signaling pathway,thereby delaying DNA damage repair.

Objective To explore the feasibility and efficacy of single-balloon enteroscope in the diagnosis and treatment of calculous small intestinal obstruction.Methods A retrospective analysis was performed on the clinical data of 16 patients who underwent endoscopic enterolith removal for small intestinal obstruction from July 2013 to December 2023.The outcomes of stone removal and therapeutic efficacy were evaluated.Results All the 16 patients successfully underwent single-balloon enteroscope.Among them,15 cases achieved successful stone extraction,while 1 case failed due to an oversized stone that could not pass through the pharyngoesophageal junction.The stone was subsequently pushed to the stomach and removed through surgical operation finally.Conclusion Single-balloon enteroscope has excellent efficacy in enterolith removal,with rapid postoperative recovery and less complications.It is worthy of widespread clinical application.

Objective:To construct a Du-moxibustion technique operation scheme suitable for patients with ankylosing spondylitis based on the "multidimensional evidence body" model, so as to provide a reference for clinical Du-moxibustion in the treatment of ankylosing spondylitis.Methods:This study was based on the "multidimensional evidence body" model, integrating evidence such as modern literature, ancient Chinese medicine books, and the experience of famous doctors, and combined with semi-structured interviews of 20 patients to form a first draft of the operation plan from April to June 2023. Twenty traditional Chinese medicine experts from 13 regions participated in the review and revision of the plan through two rounds of consultation and expert consensus meetings from July to August 2023, forming the final draft of the operation scheme.Results:Among 20 experts, there were 3 males and 17 females, aged (42.70 ± 6.57) years old. The effective response rates of the questionnaires in the two rounds of correspondence inquiries were 95%(19/20) and 18/18 respectively, and the expert authority coefficients were 0.80 and 0.81. The coefficients of variation were 0.00-0.19 and 0.00-0.21 respectively, and the Kendall harmony coefficients were 0.179 and 0.344 respectively (both P<0.01). The final scheme included 6 first-level items, including "terms and definitions""assessment""operation point""abnormal situations and handling measures""precautions""effect evaluation" and 27 second-level items and 17 third-level items. Conclusions:The operation scheme of Du-moxibustion technique is scientific, feasible and targeted, which can provide effective guidance for clinical practice and ensure the comprehensiveness and effectiveness of treatment.

Objective To explore the feasibility and efficacy of single-balloon enteroscope in the diagnosis and treatment of calculous small intestinal obstruction.Methods A retrospective analysis was performed on the clinical data of 16 patients who underwent endoscopic enterolith removal for small intestinal obstruction from July 2013 to December 2023.The outcomes of stone removal and therapeutic efficacy were evaluated.Results All the 16 patients successfully underwent single-balloon enteroscope.Among them,15 cases achieved successful stone extraction,while 1 case failed due to an oversized stone that could not pass through the pharyngoesophageal junction.The stone was subsequently pushed to the stomach and removed through surgical operation finally.Conclusion Single-balloon enteroscope has excellent efficacy in enterolith removal,with rapid postoperative recovery and less complications.It is worthy of widespread clinical application.

Objective:To investigate the effects of positive emotions,engagement,relationships,meaning and accom-plishments(PERMA)psychological intervention mode on stigma of illness and sleep quality in elderly patients with chronic heart failure.Methods:This randomized controlled study enrolled 116 elderly patients with chronic heart failure admitted to Tangdu Hospital,Air Force Medical University of PLA between May 2021 and May 2023.Pa-tients were randomly divided into control group(n=58,routine intervention)and intervention group(n=58,addi-tional PERMA mode intervention).After 2-month intervention,cardiac function[stroke volume(SV),left ven-tricular ejection fraction(LVEF),New York Heart Association(NYHA)class],stigma of illness[social impact scale(SIS)score],sleep quality[Pittsburgh sleep quality index(PSQI)score],mental state[profile of mood states-short form(POMS-SF)and Warwick-Edinburgh mental well-being scale(WEMWBS)scores]and self-efficacy[self-efficacy for managing chronic disease 6-item scale(SECD6)score]were compared between two groups.Results:Compared to patients in the control group,those in the intervention group had significantly higher SV[(69.87±5.63)ml vs.(64.33±4.21)ml],LVEF[(59.67±5.41)％vs.(50.23±5.33)％],scores of WEMWBS[(49.30±3.75)points vs.(39.64±5.83)points],SECD6[(6.80±0.60)points vs.(4.90±0.75)points](P＜0.001 all),and significantly lower scores of SIS[(58.61±4.57)points vs.(72.30±5.82)points],PSQI[(6.87±1.33)points vs.(11.54±1.78)points],POMS-SF[(122.93±4.16)points vs.(136.91±5.27)points],proportion of NYHA class Ⅲ(17.9％vs.33.9％)(P＜0.001 all).Conclusion:PERMA psychological intervention mode may relieve sigma of illness,improve heart function,sleep quality,promote the transformation of mental state and enhance self-efficacy in elderly patients with chronic heart failure.

Objective:To investigate the effects of positive emotions,engagement,relationships,meaning and accom-plishments(PERMA)psychological intervention mode on stigma of illness and sleep quality in elderly patients with chronic heart failure.Methods:This randomized controlled study enrolled 116 elderly patients with chronic heart failure admitted to Tangdu Hospital,Air Force Medical University of PLA between May 2021 and May 2023.Pa-tients were randomly divided into control group(n=58,routine intervention)and intervention group(n=58,addi-tional PERMA mode intervention).After 2-month intervention,cardiac function[stroke volume(SV),left ven-tricular ejection fraction(LVEF),New York Heart Association(NYHA)class],stigma of illness[social impact scale(SIS)score],sleep quality[Pittsburgh sleep quality index(PSQI)score],mental state[profile of mood states-short form(POMS-SF)and Warwick-Edinburgh mental well-being scale(WEMWBS)scores]and self-efficacy[self-efficacy for managing chronic disease 6-item scale(SECD6)score]were compared between two groups.Results:Compared to patients in the control group,those in the intervention group had significantly higher SV[(69.87±5.63)ml vs.(64.33±4.21)ml],LVEF[(59.67±5.41)％vs.(50.23±5.33)％],scores of WEMWBS[(49.30±3.75)points vs.(39.64±5.83)points],SECD6[(6.80±0.60)points vs.(4.90±0.75)points](P＜0.001 all),and significantly lower scores of SIS[(58.61±4.57)points vs.(72.30±5.82)points],PSQI[(6.87±1.33)points vs.(11.54±1.78)points],POMS-SF[(122.93±4.16)points vs.(136.91±5.27)points],proportion of NYHA class Ⅲ(17.9％vs.33.9％)(P＜0.001 all).Conclusion:PERMA psychological intervention mode may relieve sigma of illness,improve heart function,sleep quality,promote the transformation of mental state and enhance self-efficacy in elderly patients with chronic heart failure.

PURPOSE: This study aims to identify the prevalence and risk factors of military training-related abdominal injuries and help plan and conduct training properly. METHODS: This questionnaire survey study was conducted from October 2021 to May 2022 among military personnel from 6 military units and 8 military medical centers and participants' medical records were consulted to identify the training-related abdominal injuries. All the military personnel who ever participated in military training were included. Those who refused to participate in this study or provided an incomplete questionnaire were excluded. The questionnaire collected demographic information, type of abdominal injury, frequency, training subjects, triggers, treatment, and training disturbance. Chi-square test and t-test were used to compare baseline information. Univariate and multivariate regression analyses were used to explore the risk factors associated with military training-related abdominal injuries. RESULTS: A total of 3058 participants were involved in this study, among which 1797 (58.8%) had suffered training-related abdominal injuries (the mean age was 24.3 years and the service time was 5.6 years), while 1261 (41.2%) had no training-related abdominal injuries (the mean age was 23.1 years and the service time was 4.3 years). There were 546 injured patients (30.4%) suspended the training and 84 (4.6%) needed to be referred to higher-level hospitals. The most common triggers included inadequate warm-up, fatigue, and intense training. The training subjects with the most abdominal injuries were long-distance running (589, 32.8%). Civil servants had the highest rate of abdominal trauma (17.1%). Age ≥ 25 years, military service ≥ 3 years, poor sleep status, and previous abdominal history were independent risk factors for training-related abdominal injury. CONCLUSION More than half of the military personnel have suffered military training-related abdominal injuries. Inadequate warm-up, fatigue, and high training intensity are the most common inducing factors. Scientific and proper training should be conducted according to the factors causing abdominal injuries.
Humans ; Military Personnel ; Risk Factors ; Prevalence ; Male ; Abdominal Injuries/etiology* ; Female ; Adult ; Surveys and Questionnaires ; Young Adult

OBJECTIVE: To analyze the gene mutation characteristics and survival time of patients with newly diagnosed acute myeloid leukemia (AML) based on next-generation sequencing(NGS) gene detection. METHODS: A retrospective analysis was conducted on the clinical data of 92 patients with AML (non APL) admitted to our hospital from January 2018 to May 2022. AML related genes tested were using NGS, the mutation characteristics and survival time of AML patients were analyzed. RESULTS: Among the 92 patients, 41 were males and 51 were females. A total of 38 types of gene mutations were detected. Six-two patients carried at least one gere mutation, while no gene mutations were detected in 30 patients. In the group with favourable prognosis (n =14), the frequencies of higher gene mutations were NRAS, KIT (21.43%, n =3), KRAS (14.29%, n =2). In the group with intermediate prognosis (n =64), the gene mutation frequencies from high to low were DNMT3A (18.75%, n =12), NPM1 (17.19%, n =11), IDH2, FLT3-ITD, CEBPA (12.50%, n =8), TET2 (10.94%, n =7). In the poor prognosis group (n =14), ASXL1, TP53, EZH2, NRAS had higher gene mutation frequency than others(14.29 %, n =2 ). Statistical analysis revealed that KIT had a relative hotspot of mutations in the intermediate-risk group, and DNMT3A had a relative hotspot of mutations in the high-risk group (P < 0.05). The correlation analysis of genes with high mutation rates in different prognostic groups, such as NRAS, KIT, IDH2, DNMT3A, NPM1, and FLT3-ITD, with prognosis found that KIT was a factor affecting OS (P < 0.05), while no significant differences were observed for the others(P >0.05). CONCLUSION The frequency of gene mutations is high in AML patients, 67.4% of the patients carried at least one gene mutation. The mutation frequency varies among different genes in patients with different karyotypes, and there are obvious dominant mutations. KIT and DNMT3A can be used as factors for evaluating the prognosis of AML.
Humans ; Leukemia, Myeloid, Acute/genetics* ; Nucleophosmin ; Mutation ; Prognosis ; Retrospective Studies ; Male ; Female ; High-Throughput Nucleotide Sequencing ; Middle Aged ; DNA Methyltransferase 3A ; Adult ; Aged ; Survival Analysis ; Proto-Oncogene Proteins c-kit/genetics*

BACKGROUND: Early control of low-density lipoprotein cholesterol (LDL-C) is crucial for reducing the progress of cardiovascular disease. However, its additional role to the risk of primary osteoporosis in men with coronary heart disease was inconclusive. Our study aims to determine the association of LDL-C and its trajectories for osteoporosis risk in the middle-aged and aged men of China. METHODS: The retrospective cohort study of 1546 men aged 69.74 ± 11.30 years conducted in Beijing, China from 2015 to 2022. And the incidence of primary osteoporosis was annually recorded. LDL-C trajectories were further identified by latent class growth model using repeated measurements of LDL-C. The association of baseline LDL-C for osteoporosis was estimated using hazard ratio (HR) with 95% CI in Cox proportional hazard model, while mean level and trajectories of LDL-C for osteoporosis were evaluated using odds ratio (OR) with 95% CI in logistic regression model. RESULTS: During the median 6.2-year follow-up period, 70 men developed primary osteoporosis. The higher level of baseline LDL-C (HR = 1.539, 95% CI: 1.012-2.342) and mean LDL-C (OR = 2.190, 95% CI: 1.443-3.324) were associated with higher risk of osteoporosis in men with coronary heart disease after adjusted for covariates. Compared with those in the LDL-C trajectory of low-stable decrease, participants with medium-fluctuant trajectory, whose longitudinal LDL-C started with a medium LDL-C level and appeared an increase and then decrease, were negatively associated with osteoporosis risk (OR = 2.451, 95% CI: 1.152-5.216). And participants with initially high LDL-C level and then a rapid decrease demonstrated a tendency towards reduced risk (OR = 0.718, 95% CI: 0.212-2.437). CONCLUSIONS Elevated LDL-C level and its long-term fluctuation may increase the risk of primary osteoporosis in men. Early controlling a stable level of LDL-C is also essential for bone health.