Objective:To investigate the application value of centrifugal microfluidic chips based on loop-mediated isothermal amplification (LAMP) technology for rapid detection of foodborne pathogens.Methods:A retrospective analysis was conducted on 128 test samples collected by the Yan'an Center for Disease Control and Prevention from January 2022 to January 2024. All samples underwent bacterial culture and testing using centrifugal microfluidic chips based on LAMP technology. The detection time, spiking concentration, and accuracy of two detection methods were compared. Additionally, the diagnostic efficacy of both methods was analyzed using receiver operating characteristic (ROC) curves.Results:Bacterial cultures were mostly detected within 16-48 hours, with a detection limit concentrated at 10 000 CFU/25 g. In contrast, the LAMP-based centrifugal microfluidic chip technology typically yielded results within 5-16 hours, with a detection limit ranging from 1 000 to 10 000 CFU/25 g. The differences were statistically significant ( t = 14.86, 573.04, both P < 0.001). When compared with subsequent confirmation results, the positive rate for bacterial culture was 75.00% (24/32), with a diagnostic accuracy of 80.47% (103/128), showing a low consistency (Kappa = 0.51, P < 0.001). Meanwhile, the LAMP-based centrifugal microfluidic chip technology had a positive detection rate of 93.75% (30/32) and a diagnostic accuracy of 96.87% (124/128), also exhibiting low consistency (Kappa = 0.92, P < 0.001). In paired comparisons, the difference between the LAMP-based centrifugal microfluidic chips and bacterial culture was statistically significant ( χ2 = 53.14, P < 0.001). ROC curve analysis indicated that the area under the curve for bacterial culture in diagnosing foodborne pathogens was 0.77, while the AUC for LAMP-based centrifugal microfluidic chip technology was 0.95. Conclusions:When detecting foodborne pathogens, the use of LAMP-based centrifugal microfluidic chip technology allows for the rapid identification of pathogens in a short time period, offering advantages such as high efficiency, speed, and accuracy.

Objective:To investigate the application value of centrifugal microfluidic chips based on loop-mediated isothermal amplification (LAMP) technology for rapid detection of foodborne pathogens.Methods:A retrospective analysis was conducted on 128 test samples collected by the Yan'an Center for Disease Control and Prevention from January 2022 to January 2024. All samples underwent bacterial culture and testing using centrifugal microfluidic chips based on LAMP technology. The detection time, spiking concentration, and accuracy of two detection methods were compared. Additionally, the diagnostic efficacy of both methods was analyzed using receiver operating characteristic (ROC) curves.Results:Bacterial cultures were mostly detected within 16-48 hours, with a detection limit concentrated at 10 000 CFU/25 g. In contrast, the LAMP-based centrifugal microfluidic chip technology typically yielded results within 5-16 hours, with a detection limit ranging from 1 000 to 10 000 CFU/25 g. The differences were statistically significant ( t = 14.86, 573.04, both P < 0.001). When compared with subsequent confirmation results, the positive rate for bacterial culture was 75.00% (24/32), with a diagnostic accuracy of 80.47% (103/128), showing a low consistency (Kappa = 0.51, P < 0.001). Meanwhile, the LAMP-based centrifugal microfluidic chip technology had a positive detection rate of 93.75% (30/32) and a diagnostic accuracy of 96.87% (124/128), also exhibiting low consistency (Kappa = 0.92, P < 0.001). In paired comparisons, the difference between the LAMP-based centrifugal microfluidic chips and bacterial culture was statistically significant ( χ2 = 53.14, P < 0.001). ROC curve analysis indicated that the area under the curve for bacterial culture in diagnosing foodborne pathogens was 0.77, while the AUC for LAMP-based centrifugal microfluidic chip technology was 0.95. Conclusions:When detecting foodborne pathogens, the use of LAMP-based centrifugal microfluidic chip technology allows for the rapid identification of pathogens in a short time period, offering advantages such as high efficiency, speed, and accuracy.

The solubilization and protection of curcumin (Cur) by mixed surfactants were studied through the determination about the critical micellar concentration (CMC) of the mixed surfactants of Tween 80 and dodecyl trimethyl ammonium bromide (DTAB), molar solubilization ratio (MSR), degradation rate (k) of Cur in pH 13 solution and mixed surfactant solutions prepared at pH 13. The results showed that when Tween 80 was used alone, it exhibited high solubilization ability but poor stability. DTAB was used alone, it showed strong stability but poor solubilization ability. When DTAB was mixed with Tween 80 at different mole fractions, the stability of Cur was enhanced, and the best stability was observed when the mole fraction of DTAB was 0.4, although the solubilization ability was not the best at this mole fraction, but MSR was increased by 1.7 times compared to DTAB used alone. Mixed surfactant not only increased the solubility but also improved the stability of Cur. In addition, mixed surfactant has the advantages of less dosage and low toxicity, which is worth popularizing in application.

To study the effects of surfactants on wettability of excipients, the contact angles of six types of surfactants on the surface of two common excipients and mixture of three surfactants with excipients were measured using hypsometry method. The results demonstrated that contact angle of water on the surface of excipients was associated with hydrophilcity of excipients. Contact angle was lowered with increase in hydrophilic groups of excipient molecules. The sequence of contact angle from small to large was starch < sodium benzoate < polyvinylpyrrolidone < sodium carboxymethylcellulose < sodium alginate < chitosan < hydroxypropyl methyl cellulose Chemistry, Pharmaceutical ; Excipients ; chemistry ; Hydrophobic and Hydrophilic Interactions ; Surface-Active Agents ; chemistry ; Tablets ; Water ; Wettability