Frostbite is the most common cold injury and is caused by both immediate cold-induced cell death and the gradual development of localized inflammation and tissue ischemia. Delayed healing of frostbite often leads to scar formation, which not only causes psychological distress but also tends to result in the development of secondary malignant tumors. Therefore, a rapid healing method for frostbite wounds is urgently needed. Herein, we used a mouse skin model of frostbite injury to evaluate the recovery process after frostbite. Moreover, single-cell transcriptomics was used to determine the patterns of changes in monocytes, macrophages, epidermal cells, and fibroblasts during frostbite. Most importantly, human-induced pluripotent stem cell (hiPSC)-derived skin organoids combined with gelatin-hydrogel were constructed for the treatment of frostbite. The results showed that skin organoid treatment significantly accelerated wound healing by reducing early inflammation after frostbite and increasing the proportions of epidermal stem cells. Moreover, in the later stage of wound healing, skin organoids reduced the overall proportions of fibroblasts, significantly reduced fibroblast-to-myofibroblast transition by regulating the integrin α5β1-FAK pathway, and remodeled the extracellular matrix (ECM) through degradation and reassembly mechanisms, facilitating the restoration of physiological ECM and reducing the abundance of ECM associated with abnormal scar formation. These results highlight the potential application of organoids for promoting the reversal of frostbite-related injury and the recovery of skin functions. This study provides a new therapeutic alternative for patients suffering from disfigurement and skin dysfunction caused by frostbite.
Animals ; Organoids/metabolism* ; Mice ; Humans ; Wound Healing ; Frostbite/metabolism* ; Skin/pathology* ; Induced Pluripotent Stem Cells/cytology* ; Cicatrix/pathology* ; Fibroblasts/metabolism* ; Disease Models, Animal ; Mice, Inbred C57BL ; Extracellular Matrix/metabolism* ; Male

Objective To comprehensively analyze the reported preparation methods for animal models of perimenopausal syndrome (PS), to compare the advantages and disadvantages of various preparation elements and detection indexes, so as to provide useful references for the optimization of the relevant animal models as well as the standardization of their application in the efficacy evaluation of new drugs.MethodsIn this paper, literature research methods were applied using "perimenopausal syndrome" as the subject term. The publication period of the literature was limited to January 2016 to February 2023. Relevant literature on the preparation of PS animal models was retrieved from databases such as China National Knowledge Infrastructure, Wanfang database, and PubMed. After screening the experimental literature that met the inclusion and exclusion criteria, detailed information on experimental animal strains, modeling methods, duration of drug administration, positive drugs, detection indexes and other relevant information were collected. After the above information was standardized, the PS animal model database was established using Excel 2010 software. The model preparation elements and evaluation indexes were summarized systematically, and the statistical results were processed and analyzed using Excel 2010 software.Results A total of 247 articles were screened. SD rats (164 times, 65.86%) and Wistar rats (35 times, 14.06%) were often used to prepare PS animal models. Bilateral ovariectomy (139 times, 53.87%) and natural aging (43 times, 16.80%) were chosen as modeling methods. The ages of rats used for modeling ranged from 7 weeks to 18 months, with 3-month-old rats (22 times, 21.78%) being the most common. The detection indexes were comprehensively evaluated from multiple perspectives, including serum biochemistry, vaginal exfoliated cell smear, histomorphology, general observation, behavioral observation, and organ tissue protein immunoblotting. Western medical evaluation indexes were commonly used to test the successful preparation of models, with vaginal exfoliated cell smears being the most frequently used method (125 times, 85.04%). A model was considered successfully prepared when estrous cycle disorder or irregularity was observed. Some literature also determined modeling success by detecting a significant decrease in serum estradiol levels (5 times, 3.04%). Traditional Chinese medicine (TCM) syndrome evaluation often used a combination of Chinese and Western medical evaluation indexes for comprehensive evaluation, with researchers determining the TCM syndrome through vaginal exfoliated cell smears supplemented by general observation (3 times, 2.04%).Conclusion There are many methods for preparing PS animal models, but there are still significant differences in the selection of animal species, age, criteria for successful modeling, and TCM syndrome evaluation in the related literature.

Objective To investigate the key elements for model establishment and determine the evaluation indicators of animal models for pelvic inflammatory disease(PID),providing a reference for improving modelling methods and optimizing the application of PID animal models.Methods The search query"Pelvic Inflammatory Disease"AND"Animal Model"OR"Rat"OR"Mouse"OR"Guinea Pig"OR"Rabbit"OR"Dog"OR"Pig"was used to retrieve relevant literature on PID animal models published from 2013 to 2023 in China Knowledge Network Infrastructure(CNKI),Wanfang,and PubMed databases.The studies were analyzed and categorized based on experimental animal types,modelling methods,modelling cycles,detection indicators,positive control drugs,and administration duration.A database was established for statistical analysis.Results A total of 214 research articles on PID animal models meeting the inclusion criteria were identified.The most commonly used model animals are Sprague Dawley(SD)rats,followed by Wistar rats.The most frequently employed modelling method is a combination of mechanical injury and bacterial infection,followed by the phenol mucilage method.The most common modelling cycles for acute pelvic inflammatory disease(APID)and chronic pelvic inflammatory disease(CPID)/sequelae of pelvic inflammatory disease(SPID)are 8 to 14 days,while for PID models without specific staging,the cycles are 7 days.High-frequency detection methods and indicators include histopathological observation using hematoxylin-eosin staining,enzyme-linked immunosorbent assay(ELISA)for serum-related indicators,morphological changes of tissues observed with the naked eye,and immunohistochemical detection of related protein expression in uterine tissues,and pathological scoring.The most frequently used positive control drugs are Fuke Qianjin Tablets,followed by Jingangteng Capsules.The most common administration duration for APID is 7 days,and for CPID/SPID models,it ranges from 15 to 21 days.Conclusion Currently,SD rats and Wistar rats are commonly used as experimental animals for PID models.The dual modelling method of mechanical injury combined with mixed bacterial infection aligns closely with clinical pathogenesis and can be used to establish a PID model that simulates postoperative uterine cavity infection.Depending on the research objectives,different positive drugs and detection indicators should be selected for comprehensive evaluation.Most existing PID animal model studies are based on western medical diagnosis,with fewer studies focusing on Traditional Chinese Medicine(TCM)syndromes.There is a need to integrate TCM theories of etiology and pathogenesis to construct PID animal models that are more in line with TCM clinical symptoms.

The aryl hydrocarbon receptor(AhR),also known as dioxin receptor,is a ligand-dependent transcription factor.Because of its important role in occurrence and development of cancer,it has been widely studied.AhR is now considered to be an important regulator of host-environment interactions in immune and inflammatory responses and is involved in pathogenesis of many skin diseases.Because AhR is highly expressed in all types of skin cells and regulates many genes that are critical to skin function,it has the potential to be a new target for the treatment of inflammatory skin diseases.This paper presents and analyzes research findings on the relationship between AhR and inflammatory skin diseases to help accelerate the development of new drugs.

The incidence of augmented renal clearance (ARC) in intensive care patients (ICU) is exceptionally high, and these patients are often co-morbid with infection. The occurrence of ARC will significantly increase the clearance rate of antibiotics, making it difficult for conventional doses to reach effective therapeutic concentrations and affect the patient's anti-infective treatment effect and prognosis. It can be seen that it is crucial to formulate a reasonable dosing regimen for ICU patients with ARC. Regrettably, few reports in China about the adjustment strategy of antibiotic dosing regimens for ARC patients. Therefore, this article reviews the domestic and foreign literature for reference to provide evidence for medical personnel to adjust the dose of antibacterial drugs for such patients.

Znic (Zn) alloys with good cytocompatibility and suitable degradation rate have been a kind of biodegradable metal with great potential for clinical applications. This paper summarizes the biological role of degradable Zn alloy as bone implant materials, discusses the mechanical properties of different Zn alloys and their advantages and disadvantages as bone implant materials, and analyzes the influence of different processing strategies (such as alloying and additive manufacturing) on the mechanical properties of Zn alloys. This paper provides systematic design approaches for biodegradable Zn alloys as bone implant materials in terms of the material selection, product processing, structural topology optimization, and assesses their application prospects with a view to better serve the clinic.
Orthopedics ; Zinc ; Alloys ; Dental Materials ; Prostheses and Implants

Background The converter stations of high-voltage direct current (HVDC) transmission lines generate special total electric fields. At present, few investigations have been conducted on total electric fields in the workplace of converter stations from an perspective of occupational health. Objective To understand the current situation of total electric field strength in the workplace of converter stations. Methods Using purposive sampling, a calibrated HDEM-1 direct current (DC) total electric field strength measurement system was used to measure the total electric fields of 12 converter stations serving 6 DC lines in Southeast and Southwest China according to the Measurement method for total electric field strength and ion current density of the converter stations and DC transmission lines (DL/T 1089—2008). The results were evaluated according to occupational exposure limits recommended by The limits of electromagnetic environment at ±800 kV UHV DC converter station (DL/T 275—2012), the American Conference of Governmental Industrial Hygienists (ACGIH), and the International Commission on Non-Ionizing Radiation Protection (ICNIRP). Results A total of 615 check points were planned, the total electric field strength was 0.05-37.05 kV·m⁻¹, and the median was 10.45 kV·m⁻¹. The total electric field strength of 39 check points (6.3%) exceeded 25 kV·m⁻¹ (the limits of ACGIH and ICNIRP), and the total electric field strength of 12 check points (2.0%) exceeded 30 kV·m⁻¹ (the limit of DL/T 275—2012). There were statistically significant differences in the total electric field strength values and the proportions of exceeding 25 kV·m⁻¹ between the neutral regions and the positive regions and between the neutral regions and the negative regions (P < 0.01). The proportion of total electric field strength exceeding 30 kV·m⁻¹ in the negative regions was higher than that in the positive regions (P < 0.01). There were no significant differences in the total electric field strength of converter stations at different voltage levels and different altitudes (P > 0.05). There were no significant differences in the proportions of total electric field exceeding 25 kV·m⁻¹ and exceeding 30 kV·m⁻¹ in converter stations at different voltage levels and different altitudes (P > 0.05). Conclusion The total electric field in some workplace of converter stations exceeds selected limits. Converter station operators may be exposed to high-strength total electric field for a short time.

Objective:To analyze parents' knowledge, attitudes and practice (KAP) on antibiotics use in children.Methods:A survey of parents' KAP on antibiotics use in children was conducted among 482 parents in Tangqiao community who were visiting the vaccination unit of our center during January to June 2018. The influencing factors of patients' KAP on antibiotics use in children were investigated with univariater and multivariate logistic regression analysis.Results:In terms of knowledge, 89.0% (429/482) of parents agreed that common cold did not require antibiotics treatment; 88.8% (428/482) of parents knew that antibiotics had adverse reactions; 82.0% (395/482) of parents knew that overuse of antibiotics would increase the possibility of drug resistance of pathogenic bacteria; and 69.7% (327/482) of the relevant knowledge acquisition came from doctors. In terms of attitudes, 84.1% (405/482) of the parents agreed with the phenomenon of excessive use of antibiotics in our country; 68.2% (405/482) of the parents agreed that they should follow the doctor's instruction but not ask the doctor to prescribe drugs; 30.7% (148/482) parents thought they had enough knowledge about antibiotics drugs; 24.7% (148/482) parents were willing to use more expensive antibiotics for their children. In terms of practice, 13.9% (67/482) of parents would buy antibiotics by themselves; 35.7% (172/482) of parents had antibiotics at home. Multi-factor regression analysis results showed that parents with high annual family income are likely to better understand antibiotics ( OR=2.619, 95 %CI: 1.431—4.793); parents who had antibiotics at home ( OR=4.713, 95 %CI: 1.964—11.309)and purchased antibiotics by themselves ( OR=2.768, 95 %CI: 1.237—6.194) were more likely to use antibiotics for their children. Conclusion:There are still some problems in parents' knowledge, attitude and behavior of using antibiotics in children in the community, and public education on rational use of antibiotics in children is necessary.

OBJECTIVE: To analyze the current status of occupational exposure to power frequency electromagnetic field in converter stations. METHODS: Eight converter stations with voltage levels of ±500 kV and ±800 kV within normal operation were selected as the research subjects using the typical sampling method. Power frequency electric field and power frequency magnetic field strengths were measured and calculated according to the GBZ/T 189.3-2018 Measurement of Physical Agents in Workplace--Part 3: Electric Field and Magnetic Field between 1 Hz and 100 kHz. The GBZ 2.2-2007 Occupational Exposure Limits for Hazardous Factors in the Workplace--Part 2: Physical Factors were used to evaluate whether the power frequency electric field strength exceeds the regulatory limit(the occupational exposure limit of power frequency electric field in 8 hours workplace is 5.000 kV/m). Meanwhile, the test results were evaluated according to the short-term occupational exposure limit of 50 Hz electric field and magnetic field recommended by the International Committee on Nonionizing Radiation Protection in 2010 that are 10.000 kV/m and 1 000.00 μT. RESULTS: The power frequency electric field and magnetic field strengths of 582 working environment detection points were measured. The median and 0-100 th percentile of power frequency electric field and power frequency magnetic field strength were 4.342(0.001-12.003) kV/m and 5.51(0.10-186.90) μT, respectively.The exceeding standard rate of power frequency electric field strength in converter station workplaces was 37.8%(220/582), which concentrated in 500 kV alternating current filter area and 500 kV alternating current field area. Among them, 5 detection points had power frequency electric field strength exceeding 10.000 kV/m. The magnetic flux density of all the detection points did not exceed 1 000.00 μT. The power frequency electric field strength in ultra-high voltage region was higher than that in high voltage region(P<0.01). There was no significant difference in power frequency magnetic field strength(P>0.05). There was no significant difference in power frequency electric field and magnetic field between rectifier stations and inverter stations(P>0.05). The 8 hours time weighted average(TWA) value of power frequency electric field strength of 8 converter station operators was 1.044-2.335 kV/m, which did not exceed the occupational exposure limit. CONCLUSION: The converter station operators might be exposed to excessive power frequency electric fields for a short time, but the 8 hours TWA value of the power frequency electric field meets the requirements of standards, and the power frequency magnetic field exposure strength also meets the requirements of the relevant standards.