Objective : To investigate the role of enhancer of zeste homolog 2(EZH2)-trimethylated lysine 27 of histone H3(H3K27me3) axis in the dedifferentiation of thyroid cancer and its clinical value as a potential target for the treatment of anaplastic thyroid cancer(ATC). Methods : Immunohistochemical SP method was used to detect the expression of EZH2, H3K27me3, paired box gene 8(PAX8), thyroglobulin(TG) and thyroid transcription factor 1(TTF1) in ATC and papillary thyroid carcinoma(PTC) and their adjacent tissues. The relationship between EZH2 and thyroid differentiation markers(PAX8, TTF1, TG) was further analyzed by gene expression omnibus(GEO) database. ATC cell lines 8305C and BHT-101 were culturedin vitro. Real-time reverse transcription PCR(RT-qPCR) was used to detect the expression of thyroid differentiation markers(TTF1, PAX8) mRNA in ATC cell lines treated with EZH2 inhibitor(GSK126), and evaluate the potential therapeutic effect of GSK126in vitro. The effects of GSK126 and BRAF inhibitor vemurafenib on the proliferation of ATC cell lines were observed by cell proliferation assay. Results : The expression of EZH2 in ATC tissues was significantly higher than that in papillary thyroid carcinoma and adjacent tissues(P<0.05). The expression of H3K37me3 in ATC tissues was significantly lower than that in PTC tissues(P<0.05). EZH2 was negatively correlated with PAX8 and TG expression levels, but not with TTF1 expression level.In vitroexperiments, GSK126 could reverse the expression of thyroid differentiation markers PAX8 and TTF1 in ATC cell lines. GSK126 combined with BRAF inhibitor vemurafenib could significantly inhibit the growth of ATC cell lines. Conclusion The EZH2-H3K27me3 axis plays an important role in regulating thyroid specific markers, and the inhibition of EZH2 by small molecular compounds is a promising target for ATC treatment in the future.

Objective:To analysis effectiveness of the "14 plus 7 day quarantine" and "nucleic acid plus total antibody testing" strategy (combined screening strategy) for screenin the imported patients with COVID-19 in Xiamen.Methods:The study populations were overseas travelers arriving in Xiamen from March 17 to December 31, 2020, and overseas travelers who had quarantine outside Xiamen for less than 21 days from July 18 to December 31, 2020. Data were collected and analyzed on the timing of detection, pathways, and test results of the imported patients with COVID-19 after implementing combined screening strategy.Results:A total of 304 imported patients with COVID-19 were found from 174 628 overseas travelers and 943 overseas travelers from other cities. A total of 163 cases (53.6%) were diagnosed by multitime, multisite intensive nucleic acid testing after positive finding in total antibody testing. Among them, 27 (8.9%) were first positive for nucleic acid in 14 plus 7 day quarantine and 136 were first positive for nucleic acid in 14-day quarantine. Only 8 of these individuals were tested positive for nucleic acid after positive total antibody testing. The other 128 individuals were tested positive for nucleic acid after being negative for average 2.3 times (maximum of 6 times). Aditional 155 cases might be detected by using the combined "14 plus 7 day quarantine" and " nucleic acid plus total antibody testing" strategy compared with "14-day quarantine and nucleic acid testing" strategy, accounting for 51.0% of the total inbound infections. So the combined screening strategy doubled the detection rate for imported patients with COVID-19. No second-generation case caused by overseas travelers had been reported in Xiamen as of February 26, 2021.Conclusions:Xiamen's combined screening strategy can effectively screen the imported patients with COVID-19 who were first positive for nucleic acid after 14 day quarantine. Compared with "14 day quarantine and nucleic acid testing", the combined screening strategy improved detection rate and further reduced the risk of the secondary transmission caused by the imported patients with COVID-19.

Ethanol is one of the major risk factors for esophageal cancer.The main mechanisms of ethanol induced esophageal cancer include the direct carcinogenesis of acetaldehyde,the genetic polymorphism of enzymes related to alcohol metabolism,the carcinogenic effect of reactive oxygen species,the disorder of nutrient metabolism induced by ethanol,and the synergistic effect of ethanol and tobacco.

The aim of this study is to quantify the 146S antigen in foot-and-mouth disease virus (FMDV) inactivated vaccine by size-exclusion chromatography (SEC). The analysis was performed on a TSKgel G4000SWXL column (7.8 mm×30 cm), with a pH 7.2 buffer salt system as the mobile phase. The flow rate was 0.6 mL/min, the injection volume was 100 μL and the detection wavelength was 259 nm. The calibration curve was established by using purified inactivated FMDV (serotype O) 146S antigen; 3 batches of vaccine formulated by inactivated antigen solution were tested to verify the accuracy, reproducibility, specificity and tolerability of the method. At last 16 batches of vaccine were determined by the SEC method. Results showed a good linearity between peak area and concentration of 146S antigen in the range between 0.56 and 67.42 μg/mL (R2=0.996, n=10), and the average recovery rate of 146S antigen in the 3 batches of vaccine formulated in lab were 93.6% (RSD=2.7%, n=3), 102.3% (RSD=2.6%, n=3), and 95.5% (RSD=5.1%, n=3). The method was proved accurate and reliable with good reproducibility (RSD=0.5%, n=6), and applied to determine 16 batches of the commercial FMDV vaccine. According to the above results, the SEC method is high effective for 146S antigen quantify in the inactivated FMDV vaccine and would provide strong support for the vaccine quality control.

Syphilis can not be cultured in vitro.So far, serologic testing is still regarded as the mainstay for diagnosing syphilis and for monitoring the efficacy of subsequent antibiotic treatment.However, single serological tests have limitations in sensitivity or specificity.Detective algorithms with two or more serological methods will help to improve the effectiveness of syphilis diagnosis, and decrease missed diagnosis and misdiagnosis.This article will review advances on etiological examination, serological tests, and detective algorithms for syphilis.In particular, it specially introduces the merits and demerits of three detective algorithms for syphilis,so as to explore suitable screening methods,and provide basis for relevant administrative departments to formulate related laws, regulations and guidelines for syphilis.

OBJEVTIVETo evaluate the effect of different restoration methods on fracture resistance of endodontically treated teeth.

METHODSFifty intact extracted maxillary first premolars were randomly divided into 5 groups. Medial-occlusal cavity models were established in all the test groups (B-E) according to the same standard, followed by treatments with defect exposure only, defect filling with light cured composite resin, indirect resin inlays, or light cured composite resin combined with Biosplint fiber. Each specimen was tested using a universal test machine at 1.00 mm/min until fracture and the fracture load was recorded. The load angle was 45 degree to the long axis of the teeth, and the load was pointed to the middle of the lingual surface on the buccal cusp. The fracture resistance was analyzed statistically.

RESULTSThe mean load to cause fracture of the samples in each group group A to E was 1.27∓0.41, 0.23∓0.17, 0.55∓0.31, 0.89∓0.40, and 0.98∓0.34 kN, respectively, showing significant differences between the groups.

CONCLUSIONThe fracture resistance of the teeth is reduced after endodontic therapy, but can be increased significantly by restoration with composite resin inlay or light cured composite resin combined with Biosplint fiber.

Bicuspid ; physiopathology ; Composite Resins ; chemistry ; Dental Materials ; chemistry ; Dental Restoration, Permanent ; methods ; Dental Stress Analysis ; Humans ; Inlays ; Maxilla ; Tooth Fractures ; physiopathology ; Tooth, Nonvital ; physiopathology ; therapy

Objective To evaluate the effect of different restoration methods on fracture resistance of endodontically treated teeth. Methods Fifty intact extracted maxillary first premolars were randomly divided into 5 groups. Medial-occlusal cavity models were established in all the test groups (B-E) according to the same standard, followed by treatments with defect exposure only, defect filling with light cured composite resin, indirect resin inlays, or light cured composite resin combined with Biosplint fiber. Each specimen was tested using a universal test machine at 1.00 mm/min until fracture and the fracture load was recorded. The load angle was 45 degree to the long axis of the teeth, and the load was pointed to the middle of the lingual surface on the buccal cusp. The fracture resistance was analyzed statistically. Results The mean load to cause fracture of the samples in each group group A to E was 1.27 ± 0.41, 0.23 ± 0.17, 0.55 ± 0.31, 0.89 ± 0.40, and 0.98 ± 0.34 kN, respectively, showing significant differences between the groups. Conclusion The fracture resistance of the teeth is reduced after endodontic therapy, but can be increased significantly by restoration with composite resin inlay or light cured composite resin combined with Biosplint fiber.

Objective To evaluate the effect of different restoration methods on fracture resistance of endodontically treated teeth. Methods Fifty intact extracted maxillary first premolars were randomly divided into 5 groups. Medial-occlusal cavity models were established in all the test groups (B-E) according to the same standard, followed by treatments with defect exposure only, defect filling with light cured composite resin, indirect resin inlays, or light cured composite resin combined with Biosplint fiber. Each specimen was tested using a universal test machine at 1.00 mm/min until fracture and the fracture load was recorded. The load angle was 45 degree to the long axis of the teeth, and the load was pointed to the middle of the lingual surface on the buccal cusp. The fracture resistance was analyzed statistically. Results The mean load to cause fracture of the samples in each group group A to E was 1.27 ± 0.41, 0.23 ± 0.17, 0.55 ± 0.31, 0.89 ± 0.40, and 0.98 ± 0.34 kN, respectively, showing significant differences between the groups. Conclusion The fracture resistance of the teeth is reduced after endodontic therapy, but can be increased significantly by restoration with composite resin inlay or light cured composite resin combined with Biosplint fiber.