Objective To investigate the relationship between serum endogenous ligand(Apelin),insulin-like growth factor-2(IGF-2)glucose transporter 1(GLUT1)and tumor hyperplasia and recurrence after withdrawal propranolol in infantile hemangioma(IH)children.Methods A total of 126 IH patients were selected and divided into the proliferative phase group(74 cases)and the involuting phase group(52 cases)based on the pathological results.Meanwhile,56 patients with vascular malformations were selected as the vascular malformation group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum levels of Apelin,IGF-2 and GLUT1 on the day after admission.All IH patients were treated with propranolol for 3 months.According to the follow-up results,IH patients were divided into the recurrence group(43 cases)and the non-recurrence group(83 cases).Serum levels of Apelin,IGF-2 and GLUT1 were compared between different groups of patients.Multivariate Logistic regression analysis was used to analyze risk factors for recurrence after propranolol discontinuation in IH patients.The prediction value of serum Apelin,IGF-2 and GLUT1 levels for recurrence after propranolol discontinuation in IH patients was analyzed by receiver operating characteristic curve(ROC).Results Compared with the vascular malformation group,serum levels of Apelin,IGF-2 and GLUT1were increased in the IH group(P<0.05).Compared with the degenerative group,serum levels of Apelin,IGF-2 and GLUT1 were increased in the proliferative group(P<0.05).Serum levels of Apelin,IGF-2 and GLUT1 were significantly higher in the recurrence group than those in the non-recurrence group(P<0.05).The increased Apelin,IGF-2 and GLUT1,and hemangioma grade Ⅰ-Ⅱ after treatment were independent risk factors for recurrence after withdrawal of propranolol in children with IH(P<0.05).The area under the curve(AUC)of Apelin,IGF-2 and GLUT1 combined predicted IH recurrence was better than that of simple prediction(P<0.05).Conclusion The increased serum levels of Apelin,IGF-2 and GLUT1 in IH patients can be used as serum indicators to predict tumor hyperplasia and recurrence after propranolol withdrawal in patients.

Objective To investigate the relationship between serum endogenous ligand(Apelin),insulin-like growth factor-2(IGF-2)glucose transporter 1(GLUT1)and tumor hyperplasia and recurrence after withdrawal propranolol in infantile hemangioma(IH)children.Methods A total of 126 IH patients were selected and divided into the proliferative phase group(74 cases)and the involuting phase group(52 cases)based on the pathological results.Meanwhile,56 patients with vascular malformations were selected as the vascular malformation group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum levels of Apelin,IGF-2 and GLUT1 on the day after admission.All IH patients were treated with propranolol for 3 months.According to the follow-up results,IH patients were divided into the recurrence group(43 cases)and the non-recurrence group(83 cases).Serum levels of Apelin,IGF-2 and GLUT1 were compared between different groups of patients.Multivariate Logistic regression analysis was used to analyze risk factors for recurrence after propranolol discontinuation in IH patients.The prediction value of serum Apelin,IGF-2 and GLUT1 levels for recurrence after propranolol discontinuation in IH patients was analyzed by receiver operating characteristic curve(ROC).Results Compared with the vascular malformation group,serum levels of Apelin,IGF-2 and GLUT1were increased in the IH group(P<0.05).Compared with the degenerative group,serum levels of Apelin,IGF-2 and GLUT1 were increased in the proliferative group(P<0.05).Serum levels of Apelin,IGF-2 and GLUT1 were significantly higher in the recurrence group than those in the non-recurrence group(P<0.05).The increased Apelin,IGF-2 and GLUT1,and hemangioma grade Ⅰ-Ⅱ after treatment were independent risk factors for recurrence after withdrawal of propranolol in children with IH(P<0.05).The area under the curve(AUC)of Apelin,IGF-2 and GLUT1 combined predicted IH recurrence was better than that of simple prediction(P<0.05).Conclusion The increased serum levels of Apelin,IGF-2 and GLUT1 in IH patients can be used as serum indicators to predict tumor hyperplasia and recurrence after propranolol withdrawal in patients.

Guided bone regeneration (GBR) uses resorbable and non-resorbable membranes as biological barriers. This study compared the differences in hard tissue stability between GBR using evidence-based digital titanium mesh and resorbable collagen membranes during implant placement. A total of 40 patients (65 implant sites) were enrolled and divided into two groups: resorbable membrane and digital titanium mesh groups. The alveolar bone was analyzed at two- and three-dimensional levels using cone-beam computed tomography and by reconstructing and superimposing the hard tissues at four time points: preoperatively, postoperatively, before second-stage surgery, and 1 year after loading. The use of digital titanium mesh showed less alveolar bone resorption in vertical and horizontal directions two-dimensionally before the second-stage surgery and 1 year after loading. Regarding volumetric stability, the percentage of resorption after 6 months of healing with resorbable membrane coverage reached 37.5%. However, it was only 23.4% with titanium mesh. Although postoperative bone volume was greater at all labial sites with resorbable membrane than with digital titanium mesh, after substantial bone resorption within 1 year of loading, the labial bone thickness at the upper part of implants was thinner with resorbable membrane than with digital titanium mesh. Furthermore, digital titanium meshes made according to ideal bone arch contour reduced soft tissue irritation, and the exposure rate was only 10%. Therefore, although both resorbable membrane and digital titanium mesh in GBR were able to successfully reconstruct the bone defect, digital titanium meshes were better at maintaining the hard tissue volume in the osteogenic space.
Bone Regeneration ; Bone Resorption ; Humans ; Osteogenesis ; Surgical Mesh ; Titanium

Objective: To investigate the characteristics and prognostic value of peripheral blood T lymphocyte subsets in patients with severe influenza. Methods: This was a single-center cross-sectional study in influenza patients admitted to Peking Union Medical College Hospital from August 2017 to April 2018. Peripheral blood lymphocyte subsets were detected by flow cytometry in both patients and 108 healthy controls. Influenza patients were divided into mild group and severe group. Severe patients were further classified into alive and fatal subgroups. Results: A total of 42 influenza patients were recruited in this study, including 24 severe cases (6 deaths). The remaining 18 cases were mild. The peripheral blood lymphocyte counts and lymphocyte subset counts (B, NK, CD4⁺T, CD8⁺T) in either mild patients[795 (571,1 007), 43 (23,144), 70 (47,135), 330 (256,457), 226 (148,366) cells/μl respectively] or severe patients[661 (474,1 151),92 (52,139), 54 (34,134), 373 (235,555), 180 (105,310) cells/μl respectively] were both significantly lower than those of healthy controls [1 963 (1 603,2 394),179 (119,239), 356 (231,496), 663 (531,824), 481 (341,693) cells/μl respectively]. Meanwhile, the T cells and CD8⁺T counts in fatal patients [370 (260,537) cells/μl and 87 (74,105) cells/μl] were significantly lower than those in severe and alive patients [722 (390,990) cells/μl and 222 (154,404) cells/μl]. CD8⁺HLA-DR/CD8⁺and CD8⁺CD38⁺/CD8⁺T cell activating subgroups in mild cases[(53.7±19.2)% and 74.8% (64.1%,83.7%) respectively] were significantly higher than those in severe cases[(38.5±21.7)% and 53.3% (45.3%,67.2%) respectively].Moreover,CD8⁺HLA-DR/CD8⁺count in severe and alive group was higher than that in fatal group [(46.1±19.1)% vs. (18.2±14.6)%, P<0.01]. Logistic regression analysis showed that CD8⁺T cell count (OR=0.952, 95%CI 0.910-0.997, P=0.035) and CD8⁺HLA-DR/CD8⁺T (OR=0.916, 95%CI 0.850-0.987, P=0.022) were both negatively correlated with mortality.Peripheral blood lymphocyte counts in mild cases rapidly decreased within 1 day after diagnosis, and returned to the basic level one week later. Conclusions All peripheral blood lymphocyte subsets (T,B,NK) in patients with influenza are significantly reduced. These findings are consistent with the immunological characteristics of respiratory viral infections, in which peripheral lymphocytes (especially T cells) migrate to respiratory tract in the early stage and circulate to the peripheral blood after recovery. The activated CD8⁺T cell counts in peripheral blood are negatively correlated with the severity of disease, which could be considered as a prognostic indicator of severe influenza.

OBJECTIVE:To investigate the role of clinical pharmacists in the therapy for acute exacerbation of chronic obstruc-tive pulmonary diseases (AECOPD) complicated with pulmonary infection. METHODS:Clinical pharmacists participated in the therapy for a case of acute exacerbation of AECOPD complicated with pulmonary infection. According to the patients’signs,assis-tant examination and disease condition,clinical pharmacists provided suggestions,i.e. Piperacillin sodium and sulbactam sodium for injection (4∶1) 5.0 g,ivgtt,q12 h,for anti-infective treatment;Bisacodyl enteric-coated tablet 5 mg,po,qd,for promoting defecation;Clostridium butyricum viable bacteria tablet 20 mg,po,tid,for regulating gastrointestinal flora;Pinaverium bromide tablet 50 mg,po,tid,for regulating gastrointestinal smooth muscle tension;Shexiang baoxin pills 22.5 mg,po,tid,instead of flu-id infusion for protecting heart,and isosorbide 5-mononitrate 20 mg,po,tid,for preventing angina pectoris;Cefminox capsule 100 mg,po,tid,for anti-infective treatment;fluconazol 0.4 g,ivgtt,qd,for antifungal treatment,and Bacillus licheniformis 500 mg,po, tid,for preventing alteration of intestinal flora. RESULTS:The physicians adopted clinical pharmacist’s suggestions. No ADR was found during treatment,and the patients transferred to ordinary ward after the disease condition had been controlled. CONCLU-SIONS:The participating of clinical pharmacists in pharmaceutical care can promote rational drug use in the clinic and guarantee the safety of drug use.