Objective To analyze the infection status and subtype distribution characteristics of human papillomavirus(HPV)patients in Dongguan area from 2017 to 2023,as well as the relationship between the subtype distribution of HPV-positive patients and their cervical histopathology.Methods A total of 21 316 patients who underwent HPV genotyping in Dongguan Maternal and Maternal and Child Health Hospital of Dongguan from 2017 to 2023 were selected as the research subjects.Cervical exfoliated cells were collected from females,and specimens from lesion sites were collected from males.The 23 types of HPV genotyping were performed on the exfoliated cell specimens of the patients by PCR-reverse dot hybridization.Statistically analyze the annual prevalence of HPV subtypes from 2017 to 2023,the viral infection status of patients of dif-ferent genders and age groups,as well as the relationship between the subtype distribution of HPV-positive patients and their cervical histopathology.Results The overall detection rate of HPV was 31.5％(6 719/21 316).The annual prevalence of HPV showed a significant downward trend from 2017 to 2023(P＜0.001),and HPV types 52,16,58,53,and 51 were the most common.Female infections were mainly high-risk HPV types 52,16,58,53 and 51.Male infections were mainly low-risk HPV types 6 and 43 and high-risk HPV types 52,51 and 68.People under the age of 25 and those aged 55-＜65 were high-risk groups for infection,with detection rates of 46.6％(668/1 432)and 42.3％(473/1 117),respectively.There was a statistically significant difference in the overall detection rate among different age groups(P＜0.001).The HPV16 type increased significantly with the progression of cervical lesions from 15.0％(80/545)in patients with cervical intraepi-thelial neoplasia(CIN)Ⅰ to 36.3％(198/545)in patients with CIN Ⅱ-Ⅲ,and the difference was statistically significant(P＜0.001).HPV16 is the main infectious subtype of CIN Ⅱ-Ⅲ.Conclusion The detection rate of HPV in Dongguan area shows a downward trend from 2017 to 2023,and it is age and gender-specific.HPV 16 is closely related to high-grade CIN in HPV genotyping.