Objective To investigate the correlation between the epidermal growth factor receptor(EGFR)gene polymorphisms and non-small cell lung cancer(NSCLC)in Yunnan Han population.Methods A total of 407 NSCLC patients and 526 healthy controls were included.Five SNPs(rs1050171,rs2072454,rs2227983,rs1140475 and rs2293347)in EGFR were genotyped using TaqMan assay.The association between SNPs and NSCLC,as well as pathological types,were analyzed.Results In dominant mode,rs2072454 C/T-T/T may be a risk factor for NSCLC(P=0.004;OR=1.50,95%CI 1.14～1.96).There was a significant difference in genotype frequency between the SCC group and the control group(P=0.007),but no difference in allele frequency after Bonferroni correction(P>0.01);In the dominant mode,C/T-T/T at this locus relative to C/C was a risk for AC(P=0.002;OR=1.86,95%CI 1.24～2.80).rs1050171 A/A had a significantly higher risk of SCC in recessive mode(P=0.006,OR=2.66,95%CI 1.33～5.33).In dominant mode,rs2227983 A/G-G/G/G was a risk factor for the development of SCC relative to A/A(P=0.007;OR=1.83,95%CI 1.15～2.89).Conclusion The SNPs rs2072454,rs1050171 and rs2227983 in EGFR gene may be associated with the risk of NSCLC development as well as the type of pathology in Yunnan Han population.

To evaluate APTT for monitoring heparin neutralization and rebound following CPB. Method: Twenty patients undergoing cardiac valve replacement were randomly selected, APTT and activated clotting time (ACT) were measured 10 minutes following protamine in 0.5:1,0.7:1and 1:1 ratio to total heparin administered, and 3 and 6 hours after 1:1 ratio. Result: After the neutralization of the heparin/protamine at ratio of 0.5:1, the further doses of protamine (0.7: 1,1:1)decreased APTT markedly (P0.05). APTT with 0.5:1 or 0.7:1 ratio neutralization decreased significantly after in vitro protamine titration (P0.05). Conclusion= APTT is a better assay for monitoring both inadequate reversa. of heparin and heparin rebound,and is more sensitive to minimal dose heparin than ACT. The dose ratio of protamine to heparin at 1:1 may reverse heparin effect completely.