Objective:To investigate the effect of low expression of human epidermal growth factor-like domain protein 7 (EGFL7) gene in cervical cancer cell Hela on its migration and invasion ability.Methods:Cells in the experimental group used small interfering RNA (siRNA) to target the human EGFL7 gene to reduce the expression of EGFL7 in human cervical cancer cells Hela, and cells in the control group were transfected with Mock-siRNA. Real-time quantitative PCR was used to detect the EGFL7 mRNA content of cancer cells in each group; Western blot was used to detect EGFL7 protein expression of cancer cells in each group; The cell scratch healing experiment and Transwell experiment were used to detect the migration and invasion ability of Hela cells in each group.Results:siRNA reduced the protein expression of EGFL7 in human cervical cancer cell Hela. The wound closure percentage of Hela cells in the control group was 74.1%±6.8%. After the expression of EGFL7 was reduced, the percentage of cervical cancer cells was 42%±4.9%, and the wound closure ability was significantly reduced ( P<0.05) . The results of Transwell cell transfer showed that the number of cells successfully transferred by Hela cells in the control group was 179.24±20.01, while the number of cells successfully transferred by Hela cells with low EGFL7 expression was 79.22±13.16. The transfer ability of cells transfected with EGFL7-siRNA was significantly reduced ( P<0.05) . The results of invasion experiments showed that the number of successfully transferred cells in the control group was 79.35±8.04, the number of cells successfully transferred in the EGFL7-siRNA group was 26.98±6.24, and the invasion ability of Hela cells with low expression of EGFL7 decreased ( P< 0.05) . The expression of E-cad in Hela cells with low expression of EGFL7 was up-regulated, and the expression of MMP2/9 protein was down-regulated (all P<0.05) . Conclusion:The low expression of EGFL7 can reduce the migration and invasion ability of cervical cancer cell Hela through the EMT pathway.

Objective:To explore the effect of positive psychological intervention based on PERMA model on the fear of cancer recurrence and psychological capital in breast cancer patients during chemotherapy.Methods:A total of 60 breast cancer patients who received chemotherapy from November 2019 to January 2020 were divided into intervention group and control group with 30 cases in each group. The control group received routine health education. On this basis, the intervention group was given 8 times of positive psychological intervention. The general situation questionnaire, Fear of Progression Questionnaire-Short Form (FoP-Q-SF) and Positive Psychological Capital Questionnaire (PPQ) were used to evaluate the results of the two groups before and after intervention.Results:Before, 4 weeks after and 8 weeks after the intervention, the score of fear of cancer recurrence in the intervention group were 39.67±4.24, 34.60±3.78 and 31.17±3.60. The difference of the score of fear of cancer recurrence at different time points was statistically significant ( F value was 257.757, P<0.05). The scores of psychological capital were 113.20±19.75, 125.57±18.79 and 134.43±17.59. The difference of psychological capital level at different time points was statistically significant ( F value was 154.225, P<0.05). Conclusions:Positive psychological intervention based on PERMA model can reduce the fear of cancer recurrence in breast cancer patients during chemotherapy and improve their psychological capital level.

Objective:To explore application and effect evaluation of group prenatal care model in primiparas.Methods:A total of primiparas were recruited from December 2019 to May 2020 in the department of Obstetric clinic. Group prenatal care was carried out in the intervention group and the routine nursing was implemented in the control group. Positive capital Questionnaire and pregnancy outcome were used to evaluate the effects of intervention.Results:The scores of pre-intervention, intervention for one month and post-intervention of PPQ was (123.87±18.86), (130.70±13.41) and (142.23±8.37) respectively. Higher level of natural childbirth rate([86.7%] versus [63.3%]; χ2=4.356; P=0.037<0.05) and lower rate of perineal injury([16.7%] versus [43.3%]; χ2=5.079; P=0.024<0.05). The rate of exclusive breastfeeding during hospitalization ([90.0%] versus [66.7%]; χ2=4.812; P=0.028<0.05) after intervention as compared with those who received routine care. Conclusion:Group prenatal care intervention model can improve the level of positive psychological capital and pregnancy outcome.

Objective:To explore the effects of Group Prenatal Care (GPC) model on the fear of childbirth and pregnancy outcome of primiparas.Methods:This study was a quasi-experiment study. Totally 60 primiparas with fear of childbirth who established medical records in the Department of Obstetrics of a Class Ⅲ Grade A hospital in Hunan Province between December 2019 and April 2020 were selected by convenient sampling and divided into the intervention group and control group, with 30 cases in each group. Primiparas in the control group received routine care, while primiparas in intervention group received the central group-based pregnancy care model. The Childbirth Attitudes Questionnaire and pregnancy outcome evaluation were used to evaluate the effects in the two groups before and after intervention. SPSS 22.0 was used for data analysis.Results:After 1 month of intervention and at the end of the intervention, the total scores of fear of childbirth in the intervention group were (24.93±3.27) and (22.93±2.29) , which were lower than (35.30±8.58) and (34.47±8.70) in the control group, respectively. Repeated measurement variance analysis showed that there were temporal, grouping and interaction effects in the total scores of the two groups, and the differences were statistically significant ( Ftime=36.117, Fgroup=15.990, Finteraction=26.802; P<0.05) . The natural delivery rate in the intervention group was 86.7% (26/30) , which was higher than 63.3% (19/30) in the control group; the perineal injury rate in the intervention group was 16.7% (5/30) , which was lower than 43.3% (13/30) in the control group, and the differences were statistically significant (χ 2=4.356, 5.079; P<0.05) . There was no statistically significant difference in 2 h postpartum blood loss of primiparas and 1min Apgar score of newborns between the two groups of primipara ( P>0.05) . Conclusions:GPC model in primiparas with fear of childbirth can help to relieve the fear of childbirth in primiparas, and improve their negative pregnancy outcome, which is of significance for clinical practice.

Objective To investigate the distribution and changes of pathogens in intensive care unit of our hospital from 2010 to 2015,and provide treatment suggestions for infection.Methods The data of pathogens and multi-drug resistant organisms(MDROs)in ICU from 2010 to 2015 were analyzed retrospectively by WHONET5.6,Microsoft Office Excel2007 and SPSS19.0.Results The numbers of pathogens in ICU were increasing year by year while the top six were Acinetobacter baumanni,Pseudomonas aeruginosa,Klebsiella pneumoniae,Escherichia coli,Staphylococcus aureus and Candida albicans.The dominant MDROs were multi-drug resistant Acinetobacter baumanni,multi-drug resistant Pseudomonas aeruginosa,extended spectrum β lactamase producing Escherichia coli and Klebsiella pneumonia,methicillin-resistant coagulase negative Staph-ylococcus and methicillin-resistant Staphylococcus aureus,etc.There were statistically dramatic significant differences of most MDROs during the six years(P<0.01).Conclusion The pathogens of ICU were mainly bacteria related to the healthcare associated infection,and the multi-drug resistance was obviously in dynamic change as well as most pathogens.As a suggestion,antibiotic agents should be used rationally according to the antimicrobial susceptibility results for treating the MDROs.

Objective Measuring effects of obsessive-compulsive symptom on resting-state functional connectivity changes for children with first-episode schizophrenia. Methods Resting-state functional magnetic resonance imaging data analysis toolkit (REST) were employed to analyze resting-state functional magnetic resonance imaging data. The data were obtained from 51 children with first-episode schizophrenia and 30 healthy controls (HCG). The children with schizophrenia were divided into two groups, 21 ones with obsessive-compulsive symptom (OCG), and 30 ones without obsessive-compulsive symptom (NOCG). The right side of the medial frontal gyrus was selected as seed point to analyze its functional connectivity with the whole brain, utilizing REST. And the MNI coordinate was set as (18, 42, 27). Connectivity differences between any two groups were compared by two-sample t-test. Results Compared with HCG, functional connectivity with the left frontal lobe in OCG and NOCG was strengthened, while functional connectivity with the left precentral gyrus, the left inferior frontal gyrus, the left medial frontal gyrus, and the right frontal lobe was weakened (t=4.141,-4.223,-4.833,-4.636,-3.859, P<0.05). In NOCG, functional connectivity with the right middle occipital gyrus and parietal, the left inferior frontal gyrus and precentral gyrus was enhanced, while functional connectivity with the left lentiform nucleus, the left medial frontal gyrus, the left cingulate gyrus, the right lentiform nucleus, the right brainstem and frontal lobe was weakened(t=3.735, 4.321, 3.453, 4.393,-3.134,-3.987,-3.456,-4.739,-4.034,-4.322,P<0.05). In OCG, functional connectivity with the right middle frontal gyrus, and the left frontal lobe was enhanced, but connectivity with the right cerebellum anterior lobe, and the right precuneus was weakened (t=4.493, 4.403, 4.259,-3.513,-3.522, P<0.05). In OCG, compared with NOCG, functional connectivity with brain regions including the right middle frontal gyrus, the right lentiform nucleus, and the right inferior parietal lobule was strengthened, while functional connectivity with brain regions including the right cerebellum anterior lobe, and the right precuneus was weakened (t=4.493, 4.403, 4.259,-3.513,-3.522, P<0.05). Conclusions For children with first-episode schizophrenia with and without obsessive-compulsive symptom, there may be differences in resting-state functional connectivity disorder. Obsessive-compulsive symptom may have effect on resting-state functional connectivity changes for children in first-episode schizophrenia.

Objective Measuring effects of obsessive-compulsive symptom on resting-state functional connectivity changes for children with first-episode schizophrenia. Methods Resting-state functional magnetic resonance imaging data analysis toolkit (REST) were employed to analyze resting-state functional magnetic resonance imaging data. The data were obtained from 51 children with first-episode schizophrenia and 30 healthy controls (HCG). The children with schizophrenia were divided into two groups, 21 ones with obsessive-compulsive symptom (OCG), and 30 ones without obsessive-compulsive symptom (NOCG). The right side of the medial frontal gyrus was selected as seed point to analyze its functional connectivity with the whole brain, utilizing REST. And the MNI coordinate was set as (18, 42, 27). Connectivity differences between any two groups were compared by two-sample t-test. Results Compared with HCG, functional connectivity with the left frontal lobe in OCG and NOCG was strengthened, while functional connectivity with the left precentral gyrus, the left inferior frontal gyrus, the left medial frontal gyrus, and the right frontal lobe was weakened (t=4.141,-4.223,-4.833,-4.636,-3.859, P<0.05). In NOCG, functional connectivity with the right middle occipital gyrus and parietal, the left inferior frontal gyrus and precentral gyrus was enhanced, while functional connectivity with the left lentiform nucleus, the left medial frontal gyrus, the left cingulate gyrus, the right lentiform nucleus, the right brainstem and frontal lobe was weakened(t=3.735, 4.321, 3.453, 4.393,-3.134,-3.987,-3.456,-4.739,-4.034,-4.322,P<0.05). In OCG, functional connectivity with the right middle frontal gyrus, and the left frontal lobe was enhanced, but connectivity with the right cerebellum anterior lobe, and the right precuneus was weakened (t=4.493, 4.403, 4.259,-3.513,-3.522, P<0.05). In OCG, compared with NOCG, functional connectivity with brain regions including the right middle frontal gyrus, the right lentiform nucleus, and the right inferior parietal lobule was strengthened, while functional connectivity with brain regions including the right cerebellum anterior lobe, and the right precuneus was weakened (t=4.493, 4.403, 4.259,-3.513,-3.522, P<0.05). Conclusions For children with first-episode schizophrenia with and without obsessive-compulsive symptom, there may be differences in resting-state functional connectivity disorder. Obsessive-compulsive symptom may have effect on resting-state functional connectivity changes for children in first-episode schizophrenia.

BACKGROUND:Large numbers of experimental data have confirmed that bone marrow mesenchymal stem cel s have a positive therapeutic effect on cerebral ischemia-reperfusion injury, but there are few reports about intravenous administration of bone marrow mesenchymal stem cel conditioned medium in the treatment of stroke.
OBJECTIVE:To investigate the effects of the conditioned medium of rat bone marrow mesenchymal stem cel s on the recovery of neurological function in rats after cerebral ischemia-reperfusion injury.
METHODS:The bone marrow mesenchymal stem cel s were isolated from rat bone marrow. When cel s at passage 2 or 3 reached 90%confluence, the original culture medium was removed. Then the cel s were cultured in serum-free DMEM for 18 hours. After that, the culture solution was col ected as the conditioned medium of rat bone marrow mesenchymal stem cel s. Adult rats were subjected to 2 hours of right middle cerebral artery occlusion. Ischemia-reperfusion injury rats were randomly assigned to three groups:control group, simple culture medium group and conditioned medium group, and respectively given injection of normal saline, DMEM, conditioned medium (10 mL/kg) via the tail vein at 2, 24, 48 hours after operation.
RESULTS AND CONCLUSION:There was no difference in the behavioral tests among the three groups at postoperative 2 hours (P>0.05). Compared with the control and simple culture medium group, neurological impairment was significantly improved in the conditioned medium group at postoperative 1, 3, 5 days (P<0.05), but there was no significant difference between the control and simple culture medium groups. At postoperative 5 days, brain edema was significantly eased in the conditioned medium group in comparison to the control and simple culture medium groups (P<0.05), and there was also no difference between the latter two groups (P>0.05). These results suggest that rat bone marrow mesenchymal stem cel s-conditioned medium via intravenous administration can significantly ease brain edema and improve the neurologic function after cerebral ischemia-reperfusion injury.

Objective To investigate cognitive function and behavior characteristics in the childhood onset schizo?phrenia patients with or without obsessive-compulsive symptoms. Methods One hundred forty-nine schizophrenia chil?dren were recruited and 72 healthy children served as children control group. According to the Schedule for Affective Dis?orders and Schizophrenia for School-Age Children-Present and Lifetime Version (K-SADS-PL), the patients were fur?ther divided into two groups, the children with obsessive-compulsive disorder group (70 cases) and the children without obsessive-compulsive disorder group (79 cases). All the children were retrospectively surveyed and the Achenbach’s Child Behavior Check-list (CBCL) was used to assess their social competence and behavioral characteristics at the age from 6-year-old to 10-year-old. The cognitive function was estimated with WISC, STROOP color and word test, trail making test, visal spatial memory test and maze test. Results Compare to the control group, the CBCL factor scores of behavior problems were higher (P<0.01), social ability factor scores were lower (P<0.05) and all factor scores in the cog?nitive function tests were lower (P<0.05) in the two patients groups. Compare to the patients without obsessive-compul?sive symptoms, the CBCL factor scores of the school situation (P<0.01), splitting force (P<0.01), and physical complaints (P<0.01) were significantly higher and the scores were significantly lower in the test of common sense (P<0.01), wood (P<0.01), STROOP (P<0.01) and BVMT-R (P<0.01) in those with obsessive-compulsive symptoms. Correlation analysis showed that social competence total scores were positively correlated with similarity in the patients with obsessive-com?pulsive symptoms (r=0.31, P<0.01);while behavior problems total scores were negatively correlated with wood (r=-0.31, P<0.01) and patchwork (r=-0.32, P<0.01) in the patients without obsessive-compulsive symptoms. Conclusion The schizophrenia children with obsessive-compulsive symptoms have more behavioral problems in pre-symptom period and their cognitive dysfunction are more severe following onset of the disease. Cognitive function is related to behavioral prob?lems and social competence in the schizophrenia children with or without obsessive-compulsive symptoms .

Objective To observe whether bone marrow mesenchymal stem cell transplantation can improve vasospastic rats sense and motor function.Methods Rats grouped with randomized number method as Control group,Subarachnoid hemorrhage group.Stem cell culture media group and Stem cell transplantation group.Subarachnoid hemorrhage model were made with tail artery blood twice injection,2 days after 2' nd injection.Bone marrow mesenchymal stem cell were transplanted to lateral cistern.Subarachnoid hemorrhage(SAH) group didn' t transplant stem cell.Stem cell culture media group injected DMEM media as DMEM group.Stem cell transplantation group injected 30μl Bone Marrow mesenchymal stem cell suspension,so called BMSCs group.Neurofunctional score and learning memory expression were detected with morris mazer and Neurofunctional Score Scale in each group.Results After transplantation for 7 d,functional score of Control,SAH,DMEM and Stem cell group were 3.95 ±2.51,7.20 ± 1.03,7.23 ± 1.79 and 5.81 ± 1.11 respectively.Compared with others groups,Stem cell group score was significantly decrease(P=0.017).After transplanting stem cell for 14 d,the mean spanning plate time in Control group,SAH group,DMEM group and Stem cell group were 7.38 ± 1.73,4.52 ± 0.90,5.11 ± 1.93 and 7.32 ± 2.16 respectively,SAH and DMEM group vs other 2 groups,there were clearly statistically differences (P =0.009),while between control group and stem cell group,there were no statistically differences (P =0.14).Conclusion SAH rat transplant stem cell can improve sense,motor and learning expression in certain level.