Brain organoid-on-chip platforms have emerged as groundbreaking tools in neural disease modeling and drug discovery, offering a unique and highly accurate simulation of human organ physiology and function compared with traditional cell culture systems. This technology is a harmonious fusion of organ-on-a-chip and organoid culture technologies, leveraging their strengths to provide the most realistic in vitro replication of the in vivo environment, both physically and biologically. As both technologies continue to advance rapidly, this platform is highly promising in vitro platform for disease modeling. In this review, we summarize the historical developments, recent advancements, limitations, and future prospects of brain organoid-on-chip technology, aiming to illuminate the transformative potential of this platform in advancing our understanding and treatment of neural diseases.

Objective:To investigate the status quo of the whole process nursing needs of patients with gastrointestinal tumor surgery based on Kano model, and to provide a theoretical reference for the development of high quality nursing service.Methods:From August to September 2023, a cross-sectional survey was conducted on 284 patients undergoing gastrointestinal tumor surgery in Renji Hospital Affiliated to Shanghai Jiao Tong University by simple sampling method. Based on the Kano model as the theoretical framework, referring to the relevant literature, the questionnaire of the whole process nursing needs of patients with gastrointestinal tumor surgery was determined by two rounds of Delphi method, and the 'maximum frequency method' was used to classify the attributes of the whole process nursing needs of patients with gastrointestinal tumor surgery.Results:A total of 270 valid questionnaires were retrieved. Among 270 patients, 159 were males and 111 were females, aged 22-94 (65.34 ± 8.77) years. Among the 26 items, 16 nursing needs were expected attributes, and the first two nursing needs were pain care and relieving oral dryness. 7 essential attributes, the top 2 were relieving insomnia / drowsiness and preoperative preparation and nursing; 2 charm attributes, for postoperative follow-up and follow-up process nursing; 1 indifference attribute, which was the introduction of the surgical environment. The results of quadrant analysis showed that there were 46.15%(12/26) demands in the first quadrant (dominant area), 23.08%(6/26) in the third quadrant (observation area), and both 15.38%(4/26) in each of the other two quadrants.Conclusions:The whole process nursing needs of patients with gastrointestinal tumor surgery are diversified, and the hospital should actively meet the expected attributes and essentials of patients. On this basis, it should provide and innovate charm attribute services to improve patient satisfaction and improve the core competitiveness of the hospital.

Objective The amplitude of low-frequency fluctuation(ALFF)resting state functional magnetic resonance imaging(rs-fMRI)was used to investigate the signals image in the brain functional areas of overactive bladder(OAB)patients.Methods OAB patients treated in 3 participating hospitals during Mar.2021 and Mar.2023 were selected as the OAB group(n=14).Healthy subjects matching the gender,age and years of education of the patients in the OAB group were collected as the control group(NC group,n=14).Changes in the over active bladder symptom score(OABSS),quality of life scale(QoL),self-rating depression scale(SDS),self-rating anxiety scale(SAS)were analyzed.All subjects underwent rs-fMRI to collect blood oxygen level dependent magnetic resonance signals,which were then processed with ALFF.Two-sample t-test was conducted on the results to obtain the different brain regions.Results The OABSS[(8.07±0.37)vs.(1.21±0.18)],QoL[(4.85±0.21)vs.(0.64±0.13)],SAS[(60.14±1.40)vs.(37.64±1.57)]and SDS[(52.50±1.29)vs.(36.14±0.34)]scores of the OAB group were higher than those of the NC group,with significant differences(P＜0.05).The brain regions with significant differences in ALFF were located in the left supplementary motor area,left medial superior frontal gyrus and right anterior central gyrus(P＜0.000 1).Conclusion The abnormal spontaneous activity and coordination ability of the brain in resting state may lead to OAB symptoms,which are displayed in the abnormal functions of the left supplementary motor area,left medial superior frontal gyrus and right anterior central gyrus.

Objective:To analyze the current status of anesthesiology research in the world and China in 2023 and to identify the anesthesiology research hotspots using bibliometrics.Methods:The literature related to anesthesiology published in PubMed in 2023 was searched, and the country and author of the literature, as well as the key words of the literature were visually analyzed by using the software CiteSpace6.2. R4.Results:A total of 22 473 articles were included, the country with the largest number of publications was the United States, and China ranked second. The author with the highest number of publications in the field of anesthesiology in the worldwide in 2023 was Kaye Alan D from the United States. Chronic pain, general anesthesia and pain management were the research hotspots in the field of anesthesiology worldwide in 2023. The research hotspots in the field of anesthesiology in China focused on general anesthesia, oxidative stress and neuropathic pain.Conclusions:The United States is the leader in the research in the field of anesthesiology, with China following behind. The keywords of the literature in the field of anesthesiology between China and the world are roughly the same, reflecting the convergence of Chinese scientific research with global scientific research. Domestic anaesthesia practitioners can refer to or learn from the research hotspots of related countries and strengthen communication and cooperation between teams while conducting academic research.

Dynamic tracking analysis of monoclonal antibodies (mAbs) biotransformation in vivo is crucial, as certain modifications could inactivate the protein and reduce drug efficacy. However, a particular challenge (i.e. immune recognition deficiencies) in biotransformation studies may arise when modifications occur at the paratope recognized by the antigen. To address this limitation, a multi-epitope affinity technology utilizing the metal organic framework (MOF)@Au@peptide@aptamer composite material was proposed and developed by simultaneously immobilizing complementarity determining region (CDR) mimotope peptide (HH24) and non-CDR mimotope aptamer (CH1S-6T) onto the surface of MOF@Au nanocomposite. Comparative studies demonstrated that MOF@Au@peptide@aptamer exhibited significantly enhanced enrichment capabilities for trastuzumab variants in comparison to mono-epitope affinity technology. Moreover, the higher deamidation ratio for LC-Asn-30 and isomerization ratio for HC-Asn-55 can only be monitored by the novel bioanalytical platform based on MOF@Au@peptide@aptamer and liquid chromatography-quadrupole time of flight-mass spectrometry (LC-QTOF-MS). Therefore, multi-epitope affinity technology could effectively overcome the biases of traditional affinity materials for key sites modification analysis of mAb. Particularly, the novel bioanalytical platform can be successfully used for the tracking analysis of trastuzumab modifications in different biological fluids. Compared to the spiked phosphate buffer (PB) model, faster modification trends were monitored in the spiked serum and patients' sera due to the catalytic effect of plasma proteins and relevant proteases. Differences in peptide modification levels of trastuzumab in patients' sera were also monitored. In summary, the novel bioanalytical platform based on the multi-epitope affinity technology holds great potentials for in vivo biotransformation analysis of mAb, contributing to improved understanding and paving the way for future research and clinical applications.

Waterborne viruses that can be harmful to human health pose significant challenges globally,affecting health care systems and the economy.Identifying these waterborne pathogens is essential for preventing diseases and protecting public health.However,handling complex samples such as human and waste-water can be challenging due to their dynamic and complex composition and the ultralow concentration of target analytes.This review presents a comprehensive overview of the latest breakthroughs in waterborne virus biosensors.It begins by highlighting several promising strategies that enhance the sensing performance of optical and electrochemical biosensors in human samples.These strategies include optimizing bioreceptor selection,transduction elements,signal amplification,and integrated sensing systems.Furthermore,the insights gained from biosensing waterborne viruses in human sam-ples are applied to improve biosensing in wastewater,with a particular focus on sampling and sample pretreatment due to the dispersion characteristics of waterborne viruses in wastewater.This review suggests that implementing a comprehensive system that integrates the entire waterborne virus detection process with high-accuracy analysis could enhance virus monitoring.These findings provide valuable insights for improving the effectiveness of waterborne virus detection,which could have sig-nificant implications for public health and environmental management.

Objective:To explore the current status and research hotspots of structural equation modeling in nursing from 2012 to 2021.Methods:We searched for literature on the structural equation modeling in nursing based on Web of Science core collection database. CiteSpace and Excel were used to analyze the time, space, and keywords included in 742 articles.Results:From 2012 to 2021, the number of domestic and international publications showed a rapid growth trend with the growth of the year. China was the country with the highest number of publications. The Western University in Canada was the institution with the highest number of publications. The frequency of keywords such as structural equation modeling, quality of life, job satisfaction, health, social support and stress was high. The clustering effect of keywords was significant ( Q=0.72, S=0.87) , and research hotspots mainly included physical and mental health care and nursing work management. Conclusions:The structural equation modeling in nursing is showing a rapid development trend. In the future, numerous studies should be conducted on the construction and validation of nursing structural relationship models to promote the development of nursing.

In this paper, the domestic and international demand and development trend of clinical diagnostic radionuclides are analyzed, and the medium and high-energy cyclotrons, adequate and systematic facilities, and preparation techniques required for the production of medical radionuclides based on solid targets are introduced. This paper focuses on the research and development carried out by some important medical institutions and scientific research institutes in China over the years in the aspects of medium and high-energy cyclotrons, beam transmission lines, high-power irradiation target stations and new medical isotope production processes etc. It also looks forward to some new directions for the development of medical radionuclides in China during the 14th Five-Year Plan period.