Objective To study the protective effect of Wedelolactone(WEL)against inflammatory injury in human umbilical vein endothelial cells(HUVECs)and its molecular mechanism by inducing PI3K/Akt/mTOR.Methods The model of atherosclerosis(AS)oxidative stress injury in HUVECs was induced with 200 μmol·L-1 of hydrogen peroxide for 24 h.The experimental groups were as follows:normal control group,DMSO(dimethyl sulfoxide)group,H2O2 group,and WEL group.MTT was used to measure the cell survival rate of each group;flow cytometry was used to assess intracellular ROS levels;fluorescence microscopy was used to detect the expression of p62 protein;immunoblotting assay was used to determine the protein expression levels for apoptosis-related proteins associated with PI3K/Akt/mTOR signaling pathway and autophagy-related proteins.Results Compared with the H2 O2 group,the HUVEC cell survival rate was significantly inhibited in the WEL group(P＜0.05).ROS production was significantly lower,and the protein expressions of SOD1 and p62 were significantly increased in the WEL group as compared to the hydrogen peroxide group.The protein expression of p-mTOR,p-Akt,and p-PI3K was significantly decreased in hydrogen peroxide(P＜0.01);In the WEL experiment,p-mTOR,p-Akt,and p-PI3K were increased significantly in the post-injury HUVECs(P＜0.01).Conclusion Wedelolactone inhibits HUVECs'autophagy by suppressing H2O2-induced inflammatory damage in HUVECs,which may be related to the fact that WEL promotes the phosphorylation of PI3K,Akt,and mTOR proteins,inhibits autophagy and thus resists oxidative stress damage in HUVECs cells.

Objective To evaluate the effect of isokinetic muscle training combined with semiconductor laser on acute knee osteoarthritis (KOA). Methods Ninety-eight KOA patients were randomly divided into 4 groups. All patients were treated with conventional rehabilitation treatment and nursing. Based on that treatment, the group 1 received semiconductor laser irradiation, the group 2 received isokinetic muscle training, and the group 3 received laser irradiation combined with isokinetic muscle training. All patients were assessed with WOMAC osteoarthritis rating scale and affected knee extensor and flexor muscles strength measurement including peak torque, peak work, average power, average work and flex/extend before and 4 weeks after the treatment. Results All of the four treatments can significantly alleviate the condition of acute KOA patients and improve the muscle condition around the ipsilateral knee joint. The isokinetic muscle training improves the knee function of KOA patients better than the laser irradiation treatment. Isokinetic strength training combined with laser irradiation can get the most significant improvement of knee joint pain, stiffness, dysfunction, muscle strength of flexors and extensors in KOA patients. Conclusions The combination of isokinetic muscle training and semiconductor laser irradiation has a significant effect on relieving pain, reducing the stiffness, improving the function of knee of the patients with KOA in the acute phase and improving the muscle strength of the affected lower extremities. That methad is superior to drug therapy, physical therapy, or exercise alone, and is better to solve the problem of relieving symptoms and enhancing function simultaneously.

OBJECTIVE: To evaluate the quality of methodology and reporting of Meta-analyses on acupuncture treating ischemic stroke in China. METHODS: The domestic literature of systematic reviews (SRs) on acupuncture treating ischemic stroke was retrieved from CBM, VIP, CNKI and WANFANG databases. The quality of methodology and reporting was measured by A Measurement Tool to Assess Systematic Reviews (AMSTAR) and Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). RESULTS: Seven studies involving 6 papers in Chinese and 1 paper in English were chosen from 402 papers. The deficiencies of methodology mainly contained incomplete retrieval strategy, less attention to the publication bias and the heterogeneity, etc. The shortages of reporting was on retrieval strategy reporting, the studies features and the risk of bias reporting and evidence strength level reporting, etc. CONCLUSION At present the quality of methodology and reporting of SRs on acupuncture treating ischemic stroke in China has flaws, which needs improvement.
Acupuncture Therapy ; Brain Ischemia ; therapy ; China ; Humans ; Publication Bias ; Stroke ; therapy

OBJECTIVETo investigate the effects of long non-coding RNA(lncRNA) RP11-770J1.3 and transmembrane protein 25 (TMEM25) on paclitaxel resistance in human breast cancer MCF-7/PR cell line.

METHODSThe expression of lncRNA RP11-770J1.3 and TMEM25 in human breast cancer MCF-7(paclitaxel sensitive) and MCF-7/PR(paclitaxel resistant) cells were detected by quantitative RT-PCR. The synthetic interfering fragments of lncRNA RP11-770J1.3 and TMEM25 were transfected into MCF-7/PR cells. Sulforhodamine B assay was used to detect the sensitivity of MCF-7/PR cells to paclitaxel after interference of lncRNA RP11-770J1.3 and TMEM25. The expression of multidrug-resistance genes and proteins were detected by qRT-PCR and Western blot, respectively.

RESULTSlncRNA RP11-770J1.3 and TMEM25 were highly expressed in MCF-7/PR cells, and were significantly down-regulated after transfection of synthetic interfering fragments. Down-regulation of lncRNA RP11-770J1.3 and TMEM25 enhanced the sensitivity of MCF-7/PR cells to paclitaxel, and inhibited the expression of MRP, BCRP and MDR1/P-gp (all<0.05). Such effects were more significant when lncRNA RP11-770J1.3 and TMEM25 were both down-regulated (all<0.05).

CONCLUSIONSlncRNA RP11-770J1.3 and TMEM25 are highly expressed in MCF-7/PR cells, and the down-regulation of lncRNA RP11-770J1.3 and TMEM25 can enhance paclitaxel sensitivity in MCF-7/PR cells.

OBJECTIVETo investigate the effect of CXC chemokine receptor 4 (CXCR4) on cell cycle of breast cancer and its molecular mechanisms.

METHODSThe expression of CXCR4 and S phase kinase associated protein 2 (Skp2) was detected by real-time fluorescence quantitative PCR (fqRT-PCR) and Western blot in breast cancer cells. The expression of signal proteins and the downstream genes of Skp2 was detected by Western blot. The effect of CXCR4, PI3K/Akt pathway inhibitor LY294002 and ERK pathway inhibitor U0126 on cell cycle of breast cancer was detected by propidium iodide staining.

RESULTSSkp2 was significantly down-regulated in CXCR4-downregulated cells and up-regulated in CXCR4-upregulated cells. CXCR4 also regulated the expression of Skp2 and other downstream genes by signaling protein. The proportion of cells in G/Gphase increased and that in S phase declined in CXCR4-downregulated cell, and the effect was more significant when combined with the use of LY294002 or U0126.

CONCLUSIONSCXCR4 can affect cell cycle and inhibit the proliferation of breast cancer cells by regulating Skp2 gene expression through PI3K/Akt and ERK signaling pathway.

Aim To study the inhibitory effects of gambogenic acid in combination with miR-218 on cervical cancer HeLa cells and its mechanisms.Methods Eukaryotic expression vector of miR-218(pmi8-218) was transfected into HeLa cells.Transcript levels of miR-218 were quantified by real-time quantitative PCR.HeLa cells were incubated with different concentrations of gambogenic acid alone or in combination with pmiR-218.The cell growth inhibiting ratio of HeLa cells was assessed by MTT assay.Cell apoptosis was measured by fluorescence activated cell sorting.The expression levels of Bcl-2, Bax and E-cadherin were measured by Western blot and qRT-PCR.Results Levels of miR-218 transcript significantly increased in pmiR-218 transfected HeLa cells.Overexpression of miR-218 may enhance the sensitivity of HeLa cells to gambogenic acid.Over expression of miR-218 could enhance the effect of gambogenic acid on inhibition cell proliferation, promoting apoptosis of HeLa cells.pmiR-218 could enhance the regulation of Bax expression and decrease the expression of Bcl-2 in HeLa cells.Conclusions Over expression of miR-218 may enhance the sensitivity of HeLa cells to gambogenic acid;miR-218 can enhance the effect of gambogenic acid on inhibition cell proliferation and promote the apoptosis of HeLa cells, and the mechanism may be related to down-regulation of Bcl-2/Bax expression.

Objective To investigate the common allergens in the patients with hand eczema.Methods From November 2014 to March 2016,the patients with hand eczema were tested by the patch test kit of daily life series.Results The results of the patch test of 192 patients with hand eczema were collected.Allergens were detected in 178 (92.71％) cases.The top 5 allergens were nickel chloride (23.96％),cobalt chloride (18.75％),aromatic compounds (17.19％),nickel sulfate (16.67％) and thimerosal (13.54％).The positive rates of kappa mixture,aromatic compounds,tertiary butyl phenolic resin in males were 16.88％,14.29％,11.69％,respectively,which were higher than those (5.22％,4.35％,3.48％) in females.Conclusion Nickel chloride,cobalt chloride,aromatic compounds,nickel sulfate and thimerosal are common allergens in patients with hand eczema.

Objective To investigate the common allergens in the patients with hand eczema.Methods From November 2014 to March 2016,the patients with hand eczema were tested by the patch test kit of daily life series.Results The results of the patch test of 192 patients with hand eczema were collected.Allergens were detected in 178 (92.71％) cases.The top 5 allergens were nickel chloride (23.96％),cobalt chloride (18.75％),aromatic compounds (17.19％),nickel sulfate (16.67％) and thimerosal (13.54％).The positive rates of kappa mixture,aromatic compounds,tertiary butyl phenolic resin in males were 16.88％,14.29％,11.69％,respectively,which were higher than those (5.22％,4.35％,3.48％) in females.Conclusion Nickel chloride,cobalt chloride,aromatic compounds,nickel sulfate and thimerosal are common allergens in patients with hand eczema.

Objective To summarize the experience in nursing patients undergoing skin biopsy on head and face . Method One hundred and twenty patients with head facial dermatosis undergoing skin tissue biopsy were nursed from August 2012 to October 2013. Result All operation for biopsy were completed smoothly, with the cure rate at the first phase 100.0%, healing time 5~7 days, averaged (7.0 ± 3.0)d and no complications. Conclusion For those patients having undergone facial skin tissue biopsy, the nursing measures like psychological nursing, health education before operation, preoperative relief of mental disorder, postoperative wound nursing and health education, prevention of complications are critical for their recovery.

Objective To explore the curative effect of 308 nm excimer laser on child vitiligo and nursing measures. Method Fourty-eight children with vitiligo were treated with 308 nm excimer laser and nursed with targeted psychological treatment, skin and diet nursing.Result After treatment,6 of them got recovered(12.5%),24 remarkably improved(50%),15 slightly improved(31.2%)and 3 ineffectively treated(6.2%).Conclusions 308 nm excimer laser is effective for the treatment of child vitiligo.High quality nursing can ensure and improve the efficiency of the 308 nm excimer laser.