Objective To explore the risk factors in other vertebral new fractures after percutaneous vertebroplasty (PVP) in the patients with osteoporotic vertebral compression fractures (OVCF).Methods The pa-tients with OVCF receiving the PVP treatment in Dianjiang County People's Hospital from May 2021 to May 2022 were selected as the study subjects and their clinical data were collected.They were divided into the re-fracture group and the non-refracture group according to whether or not other vertebral developing the new fracture within postoperative one year.The data in the two groups conducted the univariate screening and mul-tivariate binary logistic regression analysis to investigate the influencing degree of different risk factors on new fracture in other vertebrae.The receiver operating characteristic (ROC) curves for the main influencing fac-tors were drawn to make the related predictive models.Results The multivariate logistic regression analysis revealed that the preoperative bone density absolute value (OR=3.693,P<0.05),bone cement type (OR=3.646,P<0.05),postoperative regular anti-osteoporosis treatment (OR=4.163,P<0.05),postoperative co-ronal Cobb angle (OR=1.017,P=0.17),number of segments during the initial surgery (OR=1.578,P<0.05) and the score of fracture degree (OR=2.002,P<0.05) were the influencing factors for the new frac-ture in other vertebrae.The ROC curve indicated that when preoperative bone density absolute value was<3.85,the risk of new fracture occurrence was up to 77.7％.Conclusion Various risk factors influence the in-cidence rate of new fracture in the other vertebrae in different degrees,the influence of preoperative bone den-sity absolute value has the greatest impact on it,so the individualized preventive measures should be developed for the patients after surgery.

PURPOSE: Osteoarthritis (OA) is a commonly occurring illness without a definitive cure, at present. Long non-coding RNAs (lncRNAs) have been widely confirmed to be involved in the modulation of OA progression. This study aimed to investigate the role and mechanism of lncRNA H19 in OA. MATERIALS AND METHODS: Abundances of H19 and microRNA-130a (miR-130a) in lipopolysaccharide (LPS)-treated C28/I2 cells were measured by reverse-transcription quantitative PCR (RT-qPCR). CCK-8 and flow cytometry analyses were carried out to assess cell viability and apoptosis. Starbase online software was used to predict the putative binding sites between H19 and miR-130a. Luciferase reporter, RNA pull down, and RT-qPCR were performed to analyze the true interaction between H19 and miR-130a. RESULTS: A notably dose-dependent elevation of H19 levels was observed in LPS-treated C28/I2 cells. Knockdown of H19 ameliorated the injury of LPS-induced C28/I2 cells, reflected by induced viability, decreased apoptosis, and reduced inflammatory factor secretions. Moreover, H19 negatively regulated the expression of miR-130a via acting as a molecular sponge for miR-130a. The stimulatory effects of H19 on cell damage were abolished following the restoration of miR-130a. CONCLUSION: LncRNA H19 aggravated the injury of LPS-induced C28/I2 cells by sponging miR-130a, hinting a novel regulatory mechanism and a potential therapeutic target for OA.
Apoptosis ; Binding Sites ; Cell Survival ; Flow Cytometry ; Luciferases ; Osteoarthritis ; Polymerase Chain Reaction ; Porifera ; RNA ; RNA, Long Noncoding ; Sincalide