AIM: To investigate the active chloroform fraction of the ethanol extract of Ipomoea carnea flowers on hematological changes in toluene diisocyanate-induced inflammation in Wistar rats. METHOD: Except for the control group, all of the rats were sensitized with intranasal application of 5 μL of 10% toluene diisocyanate (TDI) for 7 days. One week after second sensitization, all of the rats were provoked with 5 μL of 5% TDI to induce airway hypersensitivity. After the last challenge, blood and bronchoalvelor lavage (BAL) fluid were collected and subjected to total and differential leucocytes count. Flash chromatography was performed on the most active chloroform fraction to isolate an individual component. RESULTS: Treatment with the ethanolic extract and its chloroform fraction at an oral dose of 200 mg·kg⁻¹ showed a significant decrease in circulating neutrophil and eosinophil in blood and BAL as compared with standard dexamethasone (DEXA). The structure of the compound obtained from chloroform fraction of Ipomea carnea was elucidated as stigmast-5, 22-dien-3β-ol on the basis of spectral data analysis. CONCLUSION The chloroform fraction was found to be more effective to suppress airway hyper reactivity symptoms, and decreased count of both total and differential inflammatory cells.
Animals ; Asthma ; blood ; chemically induced ; drug therapy ; metabolism ; Bronchoalveolar Lavage Fluid ; Eosinophils ; metabolism ; Female ; Flowers ; chemistry ; Hematology ; Inflammation ; blood ; chemically induced ; drug therapy ; metabolism ; Ipomoea ; chemistry ; Leukocyte Count ; Male ; Molecular Structure ; Neutrophils ; metabolism ; Phytotherapy ; Plant Extracts ; chemistry ; pharmacology ; therapeutic use ; Rats ; Rats, Wistar ; Stigmasterol ; analogs & derivatives ; chemistry ; isolation & purification ; pharmacology ; therapeutic use ; Toluene 2,4-Diisocyanate

To investigate the chemical constituents of Chamaecyparis obtusa var. breviramea f. crippsii, various column chromatography and spectroscopic methods were used for the isolation and elucidation of compounds. One new monoterpenoid glucoside, (4S)-4-isopropylcyclohex-l-enecarboxylic acid 4-O-beta-D-glucopyranoside (1), together with five known compounds, (4R)-p-menth-l-ene-7, 8-diol 7-O-beta-D-glucopyranoside (2), skimmin (3), 7-[[6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranosyl]oxy]-2H-1-benzopyran-2-one (4), stigmast-4-en-3-one (5) and 1, 4-benzenedicarboxylic acid 1-butyl-4-(2-methylpropyl) ester (6) were isolated and identified from the twigs of this plant. All compounds were isolated from this plant for the first time. The methanol extract of this plant showed cytotoxicity on cancer cell lines A549, BGC-823, Du145 and MDA-MB-231 with IC50 values of 0.94, 1.07, 0.95 and 0.96 microg x mL(-1), respectively. Yet, compounds 1, 2 and 3 showed no cytotoxicity on cancer cell lines HeLa, BGC-823 and A549.
Antineoplastic Agents, Phytogenic ; chemistry ; isolation & purification ; pharmacology ; Cell Line, Tumor ; Chamaecyparis ; chemistry ; Coumarins ; chemistry ; isolation & purification ; pharmacology ; Glucosides ; chemistry ; isolation & purification ; pharmacology ; Humans ; Molecular Structure ; Monoterpenes ; chemistry ; isolation & purification ; pharmacology ; Plant Leaves ; chemistry ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Stigmasterol ; analogs & derivatives ; chemistry ; isolation & purification ; pharmacology

OBJECTIVETo study the constituents of the whole herbs of Vernonia cinerea. by bio-activity guided isolation with PC-12 model.

METHODThe constituents were separated by column chromatography and the structures were elucidated by spectroscopic methods.

RESULTFour compounds were identified to be (+)-lirioresinol B (1), stigmasterol (2), stigmasterol-3-O-beta-D-glucoside (3), 4-sulfo-benzocyclobutene (4), and their NGF inducing activity were also investigated.

CONCLUSIONCompounds 1, 3, 4 were isolated from this genus for the first time, and compound 4 was identified as a new natural product. Compounds 1, 3, 4 showed cytotoxicity on PC-12, and compounds 2, 3, 4 showed inhibition activity. Compound 4 showed a specific effect on the survival of TrkA fibroblasts, and resulted in the inducing NGF activity.

Animals ; Drugs, Chinese Herbal ; chemistry ; Glucosides ; chemistry ; Magnetic Resonance Spectroscopy ; Molecular Structure ; PC12 Cells ; Polycyclic Compounds ; chemistry ; pharmacology ; Rats ; Stigmasterol ; analogs & derivatives ; chemistry ; Vernonia ; chemistry

OBJECTIVETo study the chemical constituents of Dendobium crystallinum.

METHODCompounds were isolated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were identified by physicochemical properties and spectral analyses.

RESULTNine compounds were obtained and identified as: 4, 4'-dihydroxy-3, 5-dimethoxybi-benzyl (1), gigantol (2), naringenin (3) , p-hydroxybenzoic acid (4), n-tetracosyl trans-p-cou-marate (5), n-octacosy trans-p-coumarate (6), n-hexacosyl trans-ferulate (7), stigmasterol (8), daucosterol (9).

CONCLUSIONAll these compounds were obtained from this plant for the first time, compounds 1 and 4 were isolated firstly from the genus.

Bibenzyls ; Chromatography ; Dendrobium ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Flavanones ; chemistry ; Guaiacol ; analogs & derivatives ; chemistry ; Magnetic Resonance Spectroscopy ; Parabens ; chemistry ; Sitosterols ; chemistry ; Stigmasterol ; chemistry

OBJECTIVETo investigate the chemical constituents from the root of Psammosilene tunicoides.

METHODColumn chromatographic methods were used to isolate the chemical constituents of this plant. ESI-MS, EI-MS and NMR methods were employed for their structural elucidation.

RESULTSeven compounds were isolated and identified as goyaprosaponin (1), Soya-cerebroside (2), tectoridin (3), alpha-spinasterol (4), tetracosanoic acid (5), beta-sitosterol (6), daucosterol (7) respectively.

CONCLUSIONCompounds 2-7 were obtained from genus Psammosilene for the first time.

Caryophyllaceae ; chemistry ; Cerebrosides ; chemistry ; isolation & purification ; Isoflavones ; chemistry ; isolation & purification ; Magnetic Resonance Spectroscopy ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Spectrometry, Mass, Electrospray Ionization ; Stigmasterol ; analogs & derivatives ; chemistry ; isolation & purification

OBJECTIVETo study the chemical constituents in the fruits of Eucalyptus globulus Labill.

METHODThe chemical constituents were isolated by various column chromatographic methods and structurally elucidated by IR, NMR and MS evidences.

RESULTFifteen compounds were obtained and identified as beta-sitosterol (1), betulinic acid (2), stigmasterol (3), euscaphic acid (4), 2a-Hydroxybetulinic acid (5), macrocarpal B (6), macrocarpal A (7), oleanolic acid (8), 3,4,3'-O-trimethylellagic acid (9), 3-O-methylellagic acid 4'-O-(2"-O-acetyl )-alpha-L-rhamnopyranoside (10), camaldulenside (cypellocarpin C, 11), 3-O-methylellagic acid 4'-O-alpha-L-rhamnopyranoside (12), 3-O-methylellagic acid (13), ellagic acid (14), and gallic acid (15).

CONCLUSIONCompounds 4 and 5 from genera Eucalyptus, 1, 3 and 11 from plant E. globulus, and 6, 7, 9 and 15 from the fruits of E. globulus were isolated for the first time.

Cyclohexanecarboxylic Acids ; chemistry ; isolation & purification ; Eucalyptus ; chemistry ; Fruit ; chemistry ; Glucosides ; chemistry ; isolation & purification ; Molecular Structure ; Phloroglucinol ; analogs & derivatives ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Sesquiterpenes ; chemistry ; isolation & purification ; Sitosterols ; chemistry ; isolation & purification ; Stigmasterol ; chemistry ; isolation & purification

OBJECTIVETo study the chemical constituents from the roots of Ficus hirta.

METHODCompounds were isolated by chromatographic techniques on silica gel and HP-20 resin columns. Their structures were elucidated by chemical and spectroscopic methods.

RESULTTen compounds were identified as beta-sitosterol (1), stigmasterol (2), psoralene (3), 3beta-hydroxy-stigmast-5-en-7-one (4), 5-hydroxy-4', 6, 7, 8-tetramethoxy flavone (5), 4', 5, 6, 7, 8-pentamethoxy flavone (6), 4', 5, 7-trihydroxy-flavone (7), 3beta-acetoxy-beta-amyrin (8), 3beta-acetoxy-alpha-amyrin (9) and hesperidin (10).

CONCLUSIONThe compounds 4, 5, 6 were obtained from this genus for the first time, and all the compounds were obtained from this plant for the first time.

Ficus ; chemistry ; Ficusin ; chemistry ; isolation & purification ; Flavones ; chemistry ; isolation & purification ; Oleanolic Acid ; analogs & derivatives ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Sitosterols ; chemistry ; isolation & purification ; Stigmasterol ; chemistry ; isolation & purification

OBJECTIVETo investigate the chemical constituents of the brown alga D. divaricata, and to test cytotoxicities of the purified compounds.

METHODCompounds were isolated by normal phase silica gel, Sephadex LH-20 chromatography and reverse phase HPLC techniques. Their structures were elucidated by spectroscopic methods including IR, MS and NMR. Cytotoxicities were tested by MTT method.

RESULTEight compounds were isolated from ethanolic extract of the brown alga D. divaricata and their structures were identified as (-)-torreyol (I), 4beta, 5alpha-dihydroxycubenol (II), 3-farnesyl-p-hydroxybenzioc acid (III), chromazonarol (IV), fucosterol (V), phenyl acetylamine (VI), 4-hydroxybenzoic acid (VII) and n-hexadecanoic acid (VIII).

CONCLUSIONCompound II and IV were obtained from this alga for the first time. The others were isolated from the Dictyotaceae algae for the first time. All compounds were inactive (IC50 > 10 microg x mL(-1)) against human tumor cell lines KB, Bel-7402, PC-3M, Ketr 3 and MCF-7.

Cell Line, Tumor ; drug effects ; Humans ; Parabens ; chemistry ; isolation & purification ; pharmacology ; Phaeophyta ; chemistry ; Stigmasterol ; analogs & derivatives ; chemistry ; isolation & purification ; pharmacology ; Terpenes ; chemistry ; isolation & purification ; pharmacology ; Xanthenes ; chemistry ; isolation & purification ; pharmacology

OBJECTIVETo study the chemical constituents from the rhizome of Impatiens pritzellii var. hupehensis.

METHODThe rhizome were extracted with methanol, isolated and purified by column chromatography on silica gel. All the compounds were identified on the basis of spectral analysis (including IR, MS, NMR) and physico-chemical characters.

RESULTFive compounds were identified as a-spinasterol (I), alpha-spinasteryl-7, 22-dien-3-O-beta-D-glucopyranoside (II), stigmast-7, 22-dien-3-one (III), stearic acid (IV), hentriantane (V).

CONCLUSIONCompound I is isolated from this plant for the first time, Compound II, III, IV, V are isolated from genus Balsaminaceae for the first time.

Impatiens ; chemistry ; Plants, Medicinal ; chemistry ; Rhizome ; chemistry ; Stearic Acids ; chemistry ; isolation & purification ; Stigmasterol ; analogs & derivatives ; chemistry ; isolation & purification

AIMTo study the constituents of the root of Gypsophila oldhamiana Miq.

METHODSSilica gel column chromatographic technique was used for the isolation and purification of compounds. The structures were elucidated on the basis of spectral data and chemical evidences.

RESULTSFive compounds were obtained and identified as the beta-D-glucoside of alpha-spinasterol (I), tetracosyl caffeate (II), sucrose (III), beta-sitosterol (IV) and daucosterol (V).

CONCLUSIONCompound II is a new compound. Compound I was isolated from this plant for the first time.

Caffeic Acids ; chemistry ; isolation & purification ; Caryophyllaceae ; chemistry ; Molecular Conformation ; Molecular Structure ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Stigmasterol ; analogs & derivatives ; chemistry ; isolation & purification ; Sucrose ; chemistry ; isolation & purification