Antimicrobial resistance (AMR) is a global concern. However, in Cambodia, as in other countries in the World Health Organization’s Western Pacific Region, the magnitude of the problem is largely unknown. Thus, this study aimed to determine the prevalence of AMR in common pathogens, namely Escherichia coli and Staphylococcus aureus, isolated from blood cultures at one provincial hospital, a national sentinel site for AMR surveillance, during a 3-year period. Sample processing and analysis were conducted at the hospital’s on-site microbiology laboratory. Blood cultures were processed manually, and conventional methods were used for bacterial identification. Antibiotic susceptibility testing (AST) was performed by disk diffusion and Etest minimum inhibitory concentration measurement, in accordance with current Clinical and Laboratory Standards Institute guidelines. Blood culture data from 1 January 2020 to 31 December 2022 were extracted from the hospital’s microbiology database and, for the AST analysis, deduplicated to include results only for the first isolate per patient per year. Of 6102 blood cultures collected, 529 (9%) were positive. The most common blood culture pathogens found were E. coli (150, 28% of positive isolates) and S. aureus (65, 12% of positive isolates). For E. coli, resistance to ceftriaxone was detected in 110/148 (74%) isolates and resistance to imipenem in 3/147 (2%). For S. aureus, 18/56 (32%) isolates were methicillin-resistant, but vancomycin resistance was not detected. These rates of resistance to first-line treatments are of concern and have the potential to negatively impact patient outcomes.

Introduction: The burden of influenza in Cambodia is not well known, but it would be useful for understanding the impact of seasonal epidemics and pandemics and to design appropriate policies for influenza prevention and control. The severe acute respiratory infection (SARI) surveillance system in Cambodia was used to estimate the national burden of SARI hospitalizations in Cambodia. Methods: We estimated age-specific influenza-associated SARI hospitalization rates in three sentinel sites in Svay Rieng, Siem Reap and Kampong Cham provinces. We used influenza-associated SARI surveillance data for one year to estimate the numerator and hospital admission surveys to estimate the population denominator for each site. A national influenza-associated SARI hospitalization rate was calculated using the pooled influenza-associated SARI hospitalizations for all sites as a numerator and the pooled catchment population of all sites as denominator. National influenza-associated SARI case counts were estimated by applying hospitalization rates to the national population. Results: The national annual rates of influenza-associated hospitalizations per 100 000 population was highest for the two youngest age groups at 323 for <1 year and 196 for 1–4 years. We estimated 7547 influenza-associated hospitalizations for Cambodia with almost half of these represented by children younger than 5 years. Discussion We present national estimates of influenza-associated SARI hospitalization rates for Cambodia based on sentinel surveillance data from three sites. The results of this study indicate that the highest burden of severe influenza infection is borne by the younger age groups. These findings can be used to guide future strategies to reduce influenza morbidity.

Objective: To establish seasonal and alert thresholds and transmission intensity categories for influenza to provide timely triggers for preventive measures or upscaling control measures in Cambodia. Methods: Using Cambodia’s influenza-like illness (ILI) and laboratory-confirmed influenza surveillance data from 2009 to 2015, three parameters were assessed to monitor influenza activity: the proportion of ILI patients among all outpatients, proportion of ILI samples positive for influenza and the product of the two. With these parameters, four threshold levels (seasonal, moderate, high and alert) were established and transmission intensity was categorized based on a World Health Organization alignment method. Parameters were compared against their respective thresholds. Results: Distinct seasonality was observed using the two parameters that incorporated laboratory data. Thresholds established using the composite parameter, combining syndromic and laboratory data, had the least number of false alarms in declaring season onset and were most useful in monitoring intensity. Unlike in temperate regions, the syndromic parameter was less useful in monitoring influenza activity or for setting thresholds. Conclusion: Influenza thresholds based on appropriate parameters have the potential to provide timely triggers for public health measures in a tropical country where monitoring and assessing influenza activity has been challenging. Based on these findings, the Ministry of Health plans to raise general awareness regarding influenza among the medical community and the general public. Our findings have important implications for countries in the tropics/subtropics and in resource-limited settings, and categorized transmission intensity can be used to assess severity of potential pandemic influenza as well as seasonal influenza.