Phytochemical investigation on the leaves of Pileostegia viburnoides Hook.f.et Thoms led to the isolation of twenty-five compounds, and their structures were identified as n-dotriacontane (1), taraxeryl acetate (2), friedelin (3), epifriedelinol (4), canophyllal (5), stigmast-4-en-3-one (6), stigmasterol (7), (24R)-5A-stigmastane-3,6-dione (8), ursolic acid (9), pomolic acid (10), umbelliferone (11), 4-epifriedelin (12), n-octatriacontanol (13), β-amyrin (14), α-amyrin (15), taraxerol (16), nonadecanol (17), friedelane (18), arachic acid (19), protocatechuic acid (20), n-pentatriacontanol (21), hexadecanoic acid (22), vincosamide (23), daucosterol (24), and skimming (25), respectively. To our best knowledge, compounds 1, 2, 12, 13, 17 - 19 and 21-23 were new within Saxifragaceae family. Compounds 15, 16, and 20 were produced from this genus for the first time. Compounds 4, 14 and 25 were first obtained from species P. viburnoides and compounds 3, 5 - 11, and 24 were achieved from the leaves of P. viburnoides for the first time. Furthermore, the anti-neuroinflammatory activity of these isolates was evaluated.
Coumarins ; Humans ; Palmitic Acid ; Saxifragaceae ; Stigmasterol ; Triterpenes

Despite a low risk of liver failure and preserved liver function, non-cirrhotic hepatocellular carcinoma (HCC) has a poor prognosis. In the current study, we evaluated an active regulator of SIRT1 (AROS) as a prognostic biomarker in non-cirrhotic HCC. mRNA levels of AROS were measured in tumor and non-tumor tissues obtained from 283 non-cirrhotic HCC patients. AROS expression was exclusively up-regulated in recurrent tissues from the non-cirrhotic HCC patients (P=0.015) and also in tumor tissues irrespective of tumor stage (P<0.001) or BCLC stage (P<0.001). High mRNA levels of AROS were statistically significantly associated with tumor stage (P<0.001), BCLC stage (P=0.007), alpha fetoprotein (AFP) level (P=0.013), microvascular invasion (P=0.001), tumor size (P=0.036), and portal vein invasion (P=0.005). Kaplan-Meir curve analysis demonstrated that HCC patients with higher AROS levels had shorter disease-free survival (DFS) in both the short-term (P<0.001) and long-term (P=0.005) compared to those with low AROS. Cox regression analysis demonstrated that AROS is a significant predictor for DFS along with large tumor size, tumor multiplicity, vascular invasion, and poor tumor differentiation, which are the known prognostic factors. In conclusion, AROS is a significant biomarker for tumor aggressiveness in non-cirrhotic hepatocellular carcinoma.
Adult ; Age Distribution ; Aged ; Biomarkers, Tumor/*metabolism ; Carcinoma, Hepatocellular/*epidemiology/*metabolism/pathology ; Disease-Free Survival ; Female ; Humans ; Liver Cirrhosis/epidemiology/metabolism/pathology ; Liver Neoplasms/*epidemiology/*metabolism/pathology ; Male ; Middle Aged ; Neoplasm Invasiveness ; Nuclear Proteins/*metabolism ; Prevalence ; Reproducibility of Results ; Republic of Korea/epidemiology ; Risk Factors ; Sensitivity and Specificity ; Sex Distribution ; Transcription Factors/*metabolism ; Young Adult

Pulmonary arterial hypertension (PAH) causes right ventricular failure due to a gradual increase in pulmonary vascular resistance. The purposes of this study were to confirm the engraftment of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) placed in the correct place in the lung and research on changes of hemodynamics, pulmonary pathology, immunomodulation and several gene expressions in monocrotaline (MCT)-induced PAH rat models after hUCB-MSCs transfusion. The rats were grouped as follows: the control (C) group; the M group (MCT 60 mg/kg); the U group (hUCB-MSCs transfusion). They received transfusions via the external jugular vein a week after MCT injection. The mean right ventricular pressure (RVP) was significantly reduced in the U group after the 2 week. The indicators of RV hypertrophy were significantly reduced in the U group at week 4. Reduced medial wall thickness in the pulmonary arteriole was noted in the U group at week 4. Reduced number of intra-acinar muscular pulmonary arteries was observed in the U group after 2 week. Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4. The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining. After hUCB-MSCs were administered, there were the improvement of RVH and mean RVP. Reductions in several protein expressions and immunomodulation were also detected. It is suggested that hUCB-MSCs may be a promising therapeutic option for PAH.
Animals ; Cytokines/metabolism ; Disease Models, Animal ; Endothelin-1/metabolism ; Fetal Blood/*cytology ; Gene Expression Regulation/drug effects ; Hemodynamics ; Humans ; Hypertension, Pulmonary/chemically induced/*therapy ; Hypertrophy, Right Ventricular/physiopathology ; Immunohistochemistry ; Lung/metabolism/pathology ; Male ; Matrix Metalloproteinase 2/metabolism ; *Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells/*cytology/metabolism ; Monocrotaline/toxicity ; Nitric Oxide Synthase Type III/metabolism ; Pulmonary Artery/pathology ; Rats ; Rats, Sprague-Dawley ; Receptor, Endothelin A/metabolism

Lobectomy with mediastinal node dissection has been standard treatment for non-small cell lung cancer (NSCLC). Nowadays, video-assisted thoracoscopic surgery (VATS) is gaining acceptance as an alternative treatment option, given the quality-of-life benefits that it confers. For the VATS procedure, most surgeons create two or three ports with a utility incision of 3 to 5 cm. However, with acquired skill and instrumentation advances, single-incision thoracoscopic surgery has emerged over time. Here, we report the case of an 86-year-old female with NSCLC treated by single-incision segmentectomy.
Aged, 80 and over ; Carcinoma, Non-Small-Cell Lung* ; Female ; Humans ; Lung Neoplasms ; Mastectomy, Segmental* ; Surgical Procedures, Minimally Invasive ; Thoracic Surgery, Video-Assisted ; Thoracoscopy

OBJECTIVE: This study was done to formulate Korean fetal biometry charts of midtrimester presenting percentile values as a function of gestational age. METHODS: The relationships between the ultrasound measurement of 5 fetal parameters (biparietal diameter, head circumference, abdominal circumference, femur length, transverse cerebellar diameter) and nuchal skinfold thickness and menstrual age were determined by a cross-sectional study. The study group consisted of 215 normal healthy pregnant Korean women with known last menstrual period and regular menstrual period who had been performed genetic amniocentesis at our hospital and for whom complete pregnancy outcome information was available. All ultrasound examination was performed by one expert examiner. For each of the 5 parameters, the 5th, 10th, 25th, 50th, 75th, 90th, and 95th percentiles were determined. RESULTS: Ready-to-use fetal measurement charts in midtrimester are presented in a format giving the percentile values as a function of gestational age. CONCLUSION: These fetal biometry charts, obtained from midtrimester, Korean women can be used as a reference value.
Amniocentesis ; Biometry ; Cross-Sectional Studies ; Female ; Femur ; Fetal Development* ; Gestational Age* ; Head ; Humans ; Pregnancy ; Pregnancy Outcome ; Pregnancy Trimester, Second* ; Pregnancy* ; Reference Values ; Skinfold Thickness ; Ultrasonography*

OBJECTIVE: To assess the outcomes of pregnancies in women with kyphoscoliosis. METHOD: A total of 15 patients (17 pregnancies) complicated by kyphoscoliosis were reviewed among 19,717 deliveries between Jan. 1991 and Apr. 2001, from the Department of Obstetrics and Gynecology, Seoul National University Hospital. Their prenatal course, mode of deliveries, and pregnancy outcomes were scrutinizingly investigated. RESULTS: The incidence of kyphoscoliosis in this study was one per 1160 deliveries. The mean age of these patients in their pregnancies was 30.6+/-4.4 years (range 23-38), mean height 143.5+/-14.0 cm (range 124-160), and mean weight 55.8+/-14.4 kg (range 38-96). The causes of kyphoscoliosis included idiopathic (n=8), spinal tuberculosis (n=3), external trauma (n=3), poliomyelitis (n=1), spinal muscular atrophy (n=1), and progressive muscular dystrophy (n=1). The mean forced vital capacity (FVC) was 2.098+/-0.774 L (range 0.54-3.59) and mean vital capacity (VC) % predicted was 68.2+/-20.6% (range 24-105) prior to delivery. Vaginal delivery was performed in 4 cases, and cesarean section in 13. Fetal growth restriction was identified in 7 cases, and one case had both fetal heart anomaly and imperforate anus. Two babies were managed in neonatal intensive care unit; preterm birth at 34 weeks in one case, and term birth with low apgar score in the other. Maternal pulmonary complication was developed in two cases, the lowest two values of FVC and VC % predicted, one was FVC 0.86 L, VC % predicted 33% and the other was FVC 0.54L, VC % predicted 24%. These mothers were managed with transnasal oxygen therapy in one, artificial oxygen therapy in the other. CONCLUSION: The maternal and perinatal risks in pregnancy associated with kyphoscoliosis may be dependent on maternal pulmonary function prior to delivery.
Anus, Imperforate ; Apgar Score ; Cesarean Section ; Female ; Fetal Development ; Fetal Heart ; Gynecology ; Humans ; Incidence ; Infant, Newborn ; Intensive Care, Neonatal ; Mothers ; Muscular Atrophy, Spinal ; Muscular Dystrophies ; Obstetrics ; Oxygen ; Poliomyelitis ; Pregnancy Outcome ; Pregnancy* ; Premature Birth ; Seoul ; Term Birth ; Tuberculosis, Spinal ; Vital Capacity

OBJECTIVE: To assess the outcomes of pregnancies in women with kyphoscoliosis. METHOD: A total of 15 patients (17 pregnancies) complicated by kyphoscoliosis were reviewed among 19,717 deliveries between Jan. 1991 and Apr. 2001, from the Department of Obstetrics and Gynecology, Seoul National University Hospital. Their prenatal course, mode of deliveries, and pregnancy outcomes were scrutinizingly investigated. RESULTS: The incidence of kyphoscoliosis in this study was one per 1160 deliveries. The mean age of these patients in their pregnancies was 30.6+/-4.4 years (range 23-38), mean height 143.5+/-14.0 cm (range 124-160), and mean weight 55.8+/-14.4 kg (range 38-96). The causes of kyphoscoliosis included idiopathic (n=8), spinal tuberculosis (n=3), external trauma (n=3), poliomyelitis (n=1), spinal muscular atrophy (n=1), and progressive muscular dystrophy (n=1). The mean forced vital capacity (FVC) was 2.098+/-0.774 L (range 0.54-3.59) and mean vital capacity (VC) % predicted was 68.2+/-20.6% (range 24-105) prior to delivery. Vaginal delivery was performed in 4 cases, and cesarean section in 13. Fetal growth restriction was identified in 7 cases, and one case had both fetal heart anomaly and imperforate anus. Two babies were managed in neonatal intensive care unit; preterm birth at 34 weeks in one case, and term birth with low apgar score in the other. Maternal pulmonary complication was developed in two cases, the lowest two values of FVC and VC % predicted, one was FVC 0.86 L, VC % predicted 33% and the other was FVC 0.54L, VC % predicted 24%. These mothers were managed with transnasal oxygen therapy in one, artificial oxygen therapy in the other. CONCLUSION: The maternal and perinatal risks in pregnancy associated with kyphoscoliosis may be dependent on maternal pulmonary function prior to delivery.
Anus, Imperforate ; Apgar Score ; Cesarean Section ; Female ; Fetal Development ; Fetal Heart ; Gynecology ; Humans ; Incidence ; Infant, Newborn ; Intensive Care, Neonatal ; Mothers ; Muscular Atrophy, Spinal ; Muscular Dystrophies ; Obstetrics ; Oxygen ; Poliomyelitis ; Pregnancy Outcome ; Pregnancy* ; Premature Birth ; Seoul ; Term Birth ; Tuberculosis, Spinal ; Vital Capacity

The incidence of brain metastasis from the ovarian cancer is increasing because of the increased survival rate of the ovarian cancer patients and new development of the diagnostic imaging study. The traditional treatment of the brain metastasis is whole brain radiation therapy and recent trend of the treatment is multidisciplinary treatment (radiation therapy, surgery, radiosurgery, and chemotherapy). Recently we experienced a case with multiple brain metastases from the ovarian cancer which were treated by a multimodality approach including gamma-knife radiosurgery. She was diagnosed as epithelial ovarian cancer, FIGO stage IIIc, and underwent 9 cycles of cisplatin based combination chemotherapy (cyclophospamide, THP, cisplatin) after optimal cytoreductive surgery with Hartmans operation. Complete remission was achieved, but multiple brain metastases were detected 56 months after the initial diagnosis of ovarian cancer. She had no recurrent lesion outside the brain, and underwent surgical resection of the one respectable lesion and then gamma-knife radiosurgery for another unresectable lesion. She remains in a disease-free state without any symptoms for 4 months. We report this case with a brief review of the concerned literatures.
Brain* ; Cisplatin ; Diagnosis ; Diagnostic Imaging ; Drug Therapy, Combination ; Humans ; Incidence ; Neoplasm Metastasis* ; Ovarian Neoplasms* ; Radiosurgery ; Survival Rate

Strumal carcinoid of ovary is an unusual form of monodermal ovarian teratoma with thyroid-like follicles admixed with typical carcinoid tumor pattern. Most of them arise in dermoid cyst or in mature solid teratoma. Recently we experienced a case of strumal carcinoid postoperatively and report this case with a brief review of the concerned literatures.
Carcinoid Tumor* ; Dermoid Cyst ; Female ; Ovarian Neoplasms ; Ovary* ; Teratoma

No abstract available.
Antibodies* ; Hemorrhagic Fever with Renal Syndrome* ; Humans* ; Immunization*