ObjectiveUltra performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry （UPLC-Q-TOF-MS/MS） coupled with network pharmacology and molecular docking was utilized to explore the efficacy and mechanism of action of Ermiao Situ decoction on rats with damp-heat eczema. MethodsA rat model of damp-heat eczema was established by artificial climate chamber intervention combined with sensitization induction by dinitrochlorobenzene （DNCB）， and it was randomly divided into the normal group， the model group， the medium- and high-dose groups of Ermiao Situ decoction （3.40 g·kg^-1 and 6.80 g·kg^-1）， and the prednisone acetate group （2.51 mg·kg^-1）， with eight rats in each group， totalling 46 rats， of which six rats were tested with the drug-containing serum. The chemical analysis of drug-containing serum from rats was carried out by UPLC-Q-TOF-MS/MS， combined with network pharmacology for the prediction of key components， core targets， and signaling pathways， and molecular docking experiments were performed by CB-Dock2 online website. The pharmacological effects of Ermiao Situ decoction in the treatment of damp-heat eczema were investigated by epitaxial indexes combined with the pathologic tissue staining method. The serum levels of gastrin （GAS）， interleukin-4 （IL-4）， and interleukin-13 （IL-13） were measured by enzyme-linked immunosorbent assay （ELISA）. Interleukin-6 （IL-6）， Janus kinase 1 （JAK1）， phosphorylated （p）-JAK1， signal transduction and activation of transcription factor 3 （STAT3）， and p-STAT3 protein expression level was determined by Western bolt. ResultsA total of 19 active ingredients were detected in drug-containing serum samples of rats， which were predicted to act on 198 targets for the treatment of damp-heat eczema， among which the key ingredients included rhodopsin， huangpai alkaloids， and quercetin， and the main core targets included STAT3， tumor necrosis factor （TNF）， and IL-6， which were mainly involved in the cancer signaling pathway， phosphatidylinositol 3-kinase （PI3K）/protein kinase （Akt） signaling pathway， T helper 17 （Th17） cell differentiation signaling pathway， and JAK/STAT signaling pathway. The molecular docking results suggested that the key components had strong binding activities with the core targets IL-6， JAK1， and STAT3 in the JAK/STAT signaling pathway. The results of animal experiments showed that compared with those in the normal group， rats in the model group were depressed. They had loose hair， loose stools， epidermal oozing， vesiculation， and generation of thick scabs in the form of scales， decreased body weight， increased anus temperature and water intake， and increased indexes of the spleen， thymus gland， and stomach （P<0.05， P<0.01）， and the lesion tissue could be seen to be hyperkeratotic， with the aggregation of inflammatory cells and nonsignificant separation of epidermis and dermis. The gastric mucosa was thinned， deficient， and structurally disorganized， and obvious inflammatory cell aggregation was seen. The levels of GAS， IL-4， and IL-13 in serum were significantly reduced （P<0.05， P<0.01）， and the protein expression levels of IL-6， JAK1， p-JAK1， and p-STAT3 in the lesion tissue were significantly increased （P<0.05， P<0.01）. Compared with those in the model group， rats in each administration group had stable mental states， formed feces， a clean perianal area， and basically normal epidermis. Only a small amount of scaly scabs existed， and the rats had body weight increased， with decreased anal temperature and water intake， as well as decreased spleen， thymus， and gastric indexes （P<0.05， P<0.01）. Epidermal thickness was decreased， and epidermal and dermal separation boundaries were obvious， but hyperkeratotic and accumulation of inflammatory cells could still be seen. The thickness of gastric mucosa increased， and the structure was restored to varying degrees. The levels of GAS， IL-4， and IL-13 content in the serum of rats were increased to varying degrees， and the protein expression levels of IL-6， JAK1， p-JAK1， and p-STAT3 in the dermal lesion tissue were significantly decreased （P<0.05， P<0.01）. ConclusionErmiao Situ decoction may exert therapeutic effects on rats with damp-heat eczema by modulating the JAK/STAT signaling pathway.

OBJECTIVE To investigate the antidepressant mechanism of Shugan hewei tang （SGHWT） based on the metabolomics of prefrontal cortex （PFC）-nucleus accumbens （NAc）-ventral tegmental area （VTA） neural circuit. METHODS Male SD rats were randomly divided into blank group， model group， SGHWT low-， medium- and high-dose groups [3.67， 7.34， 14.68 g/（kg·d）， by raw material]， and fluoxetine group [1.58 mg/（kg·d）， positive control]， with 12 rats in each group. Except for the blank group， the depression model was established by chronic unpredictable mild stress combined with individual cage housing in the remaining groups， and the corresponding drug solution or normal saline was administered via gavage during modeling， once a day， for 6 consecutive weeks. After the last administration， the body weight， sucrose preference rate， total moving distance， frequency into the center and immobility time of rats in each group were detected. Samples of PFC， NAc and VTA areas of rats in the blank group， model group， SGHWT medium-dose group and fluoxetine positive control groups were collected，and their histomorphological features were observed， and non-targeted metabolomics analysis （except for fluoxetine group）were performed and validated. RESULTS Compared with model group， the cytolysis， structural damage and other pathological damages in three brain regions of rats were significantly alleviated in each drug group， while their body weight， sucrose preference rate， total moving distance and frequency into the center were all significantly higher or longer （P＜0.05）， and immobility time was significantly shorter （P＜0.05）. The results of non-targeted metabolomics showed that a total of 78 endogenous differential metabolites were identified， with 40， 35 and 24 in the PFC， NAc and VTA regions respectively， mainly involved in amino acid， lipid and sphingolipid metabolism. The results of metabolic pathway enrichment analysis showed that SGHWT affected the neural circuits of depressed rats by regulating sphingolipid metabolism， alanine， aspartic acid and glutamic acid metabolism， saturated fatty acid biosynthesis， among which alanine， aspartic acid and glutamic acid metabolism was predominantly involved. Validation experiments showed that SGHWT significantly increased the phosphorylation levels of protein kinase B （Akt） and mammalian target of rapamycin （mTOR）， and decreased the protein expression of N-methyl-D-aspartic acid receptor 1 （NMDAR1） in the NAc region of rats. CONCLUSIONS SGHWT significantly improves the depression-like behavior and attenuates pathological damage of PFC-NAc-VTA neural circuit of model rats， the mechanism of which is associated with inhibiting NMDAR1 expression and activating the Akt/mTOR signaling pathway.

Objective The aim of this study was to investigate the expression of nuclear pore membrane protein 121(POM121)in head and neck mucosal melanoma(HNMM)and its effect on the migration and invasion of HNMM cells.Methods The cancer tissues from 63 patients with HNMM and 20 adjacent normal oral epithelial tissues who were treated in the Harbin Cancer Hos-pital of Harbin Medical University from March 1,2011 to March 30,2015 were collected,and the expression of POM121 was detected by immunohistochemistry.The relationship between the expression of POM121 and the clinicopathological features of HNMM patients and its effect on the prognosis of patients were analyzed.Western blot was used to detect the expression of POM121 in HNMM cells(MM9H-1 cells)and human oral epithelial cells(human oral keratinocytes,HOK cells).After knocking down POM121 with small in-terfering RNA(siRNA),the effects on the migration and invasion of HNMM cells were detected by Scratch assay and Transwell assay.Results Immunohistochemistry showed that expression of POM121 was significantly higher in HNMM tissues than that in adjacent tissues(P<0.001).The expression of POM121 was associated with tumor size and stage(P<0.05).Kaplan-Meier analysis showed that the disease-free survival(DFS)and overall survival(OS)of patients with low expression of POM121 were significantly longer than those with high expression of POM121.Cox regression analysis showed that POM121 expression,AJCC stage and lymph node metasta-sis were influencing factors for OS and DFS of HNMM patients(P<0.05).Western blot results showed that the expression of POM121 protein in MM9H-1 cells was significantly higher than that in HOK cells(P<0.05).After knocking down POM121,the cell migration rate and cell invasion ability were significantly reduced(P<0.001).Conclusion POM121 is highly expressed in HNMM tissues and cells,and down-regulation of POM121 significantly inhibits the migration and invasion of HNMM cells.

Objective:To explore the effect of autologous vascular graft bridging of the popliteal artery defect to reconstruct the blood supply of leg on survival of lower extremity.Methods:From May 2021 to May 2024, 10 patients with traumatic popliteal artery injury with defect were treated in the Department of Hand and Foot Surgery, Nanhua Hospital Affiliated to University of South China. A retrospective analysis was conducted on clinical data of the patients. Injury sites were: 6 in left lower extremity and 4 in right lower extremity. Causes of injury were: 4 of traffic accident, 2 of heavy object compression, 2 of electric saw, and 2 of falling from heights. Sixe patients had large vessel injury combined with fracture and (or) knee dislocation, and 4 had simple large vessel injury. Six patients had open vascular injury and 4 had closed vascular injury. There were 6 popliteal artery defect and 4 femoral artery defect, with the defects ranging from 5.0 to 10.0 cm in length. Based on the condition of the arterial defect, autologous great saphenous vein (9 patient) and small saphenous vein (1 patient) were taken and prepared to different forms of vascular bridging graft, such as "single crotch-shape" (6 patient), "double crotch-shape" (3 patient) and "triple crotch-shaped" (1 patient) vascular bridging grafts, to have the defects of popliteal artery reconstructed. Scheduled postoperative follow-up was conducted at outpatient clinic and via telephone interviews. Detailed outcomes including the peripheral blood flow, presence of necrosis or ulcer as well as patient satisfaction were recorded.Results:All of the blood vessel anastomoses in 10 patients were successful and the distal blood supply of the affected legs was restored. All of the affective legs survived. Postoperative follow-ups at 3 to 12 months showed good blood flow in the legs without necrosis nor ulcer as well as a high patient satisfaction.Conclusion:The "crotch-shaped" autologous blood vessel graft can timely and effectively reconstruct defected main artery in popliteal fossa, reduce ischemia time of the affected leg, promote extremity survival and restore its function.

Inguinal hernia is a common general surgical non-oncological condition,with more than 20 million inguinal hernia surgeries performed annually worldwide.Surgery is the only treatment for inguinal hernias in adults due to the lack of spontaneous healing and ineffective pharmacological treatment,as well as the risk of life-threatening intestinal necrosis due to herniorrhaphy.The incidence of inguinal hernia is on the rise,and there may be an association between its pathogenesis and the mechanism of sarcopenia,as well as the adverse effects of sarcopenia on various postoperative conditions.This paper aims to investigate the relationship between the recognition,diagnosis,treatment,pathogenesis,early postoperative clinical recovery and recurrence of sarcopenia in patients with inguinal hernia.

The triceps surae muscle-tendon unit(MTU)is the key for effective force generation,transmission,and energy storage and release in human movement,with great influences on sports efficiency,but its injury rate remains high.This review summarizes MTU function and its biomechanical adaptations during running,aiming to deepen the understanding of MTU's role in movement and explore how external factors influence its biomechanical properties,offering scientific insights for improving running performance and preventing injuries.During running,under forefoot striking and wearing shoes with greater stiffness,such as shoes with carbon plate,the energy contraction or return could be achieved through a more economical MTU behavior,namely reducing energy consumption by more economically muscle contraction and more energy storage of elastic elements,and the muscle contraction was exerted at a length closer to the optimal muscle fascicle length and reduced energy consumption caused by muscle contraction.However,adopting forefoot striking,barefoot running,or wearing minimalist shoes during running could increase the mechanical loads on the MTU.After gait retraining,the MTU could contract or return energy more efficiently during running,while the impact of other training methods on MTU was relatively poor,and the research in this area is still not sufficient.Therefore,future research should focus on optimizing the biomechanical properties of the MTU,MTU interaction and balanced development through changes in movement patterns,equipment,and training methods to enhance performance and reduce injuries.

Objective To compare the value of standard deviation(SD)method and root mean square(RMS)method for measuring signal-to-noise ratio(SNR)of MRI of American College of Radiology(ACR)phantom based on phased array coils.Methods ACR phantom was scanned with head coil(Head),abdominal coil(Anterior),dual piece flexible coil(Flex L)and abdominal coil+flexible coil(Anterior+Flex L)each for 6 times,respectively,with sequences of axial T 1W-spin echo(SE)and T2W-SE.And the scanning schemes were divided into 8 groups based on 4 types of coils and 2 sequences.SNR of 8 groups were measured with standard deviation method and root mean square method,respectively,and the differences between the above 2 methods were observed.Results For T1W-SE sequence,SNRsD of MRI based on Head,Anterior,Flex L and Anterior+Flex L was 1.25±0.07,0.56±0.02,1.15±0.10 and 1.04±0.11,respectively,while SNRRMs was 1.10±0.07,0.58±0.03,1.01±0.13 and 1.31±0.15,respectively.For T2W-SE sequence,SNRsD was 1.40±0.08,0.48±0.02,1.04±0.07 and 1.08±0.06,respectively,while SNRRMs was 1.29±0.09,0.53±0.03,0.84±0.08 and 1.35±0.12,respectively.Compared pairwise,significant difference of SNRsD was found in 9 pairs(all P＜0.05),while of SNRRMs was detected in 10 pairs(all P＜0.05).Conclusion Compared with standard deviation method,root mean square method was more suitable for measuring SNR of MRI of ACR phantom based on phased array coils.

Objective To explore the effect of exercise-induced fatigue on intra-and interhemispher-ic functional connectivity of the primary motor cortex during unilateral ankle plantar-dorsiflexion.Meth-ods Twenty-four healthy adult males(age:21.6±2.2 years;all right-side dominant)were selected as participants for the study.They ran on a treadmill at an individualized constant speed until fatigue.Fatigue was determined when all the following criteria were met:(1)participants failing to maintain the individualized constant speed despite the strong verbal encouragement from the experimenters;(2)reaching a rating of perceived exertion(RPE)score of at least 19;and(3)their heart rate reaching 90%of their age-predicted maximal heart rate(220-age).Moreover,EEG signals from four channels(C1,C2,C3,and C4)of the primary motor cortex were collected during unilateral ankle dorsi-plan-tarflexion movements before and after fatigue,followed by EEG coherence analyses on the collected da-ta.Results Compared to the pre-fatigue state,the coherence values of the intra-hemispheric primary motor cortex electrode pairs C1-C3 and C2-C4 during unilateral ankle plantar-dorsiflexion decreased significantly in the alpha and gamma bands after fatigue(both P<0.05).Conversely,the value of the interhemispheric primary motor cortex electrode pair C1-C2 increased significantly in the beta band(P<0.05).Conclusion Exercise-induced fatigue significantly weakens the intra-hemispheric functional con-nectivity of the primary motor cortex during unilateral ankle plantar-dorsiflexion.However,it may en-hance the interhemispheric functional connectivity and potentially collaborate to strengthen the regula-tion of fatigued muscles.

Inguinal hernia is a common general surgical non-oncological condition,with more than 20 million inguinal hernia surgeries performed annually worldwide.Surgery is the only treatment for inguinal hernias in adults due to the lack of spontaneous healing and ineffective pharmacological treatment,as well as the risk of life-threatening intestinal necrosis due to herniorrhaphy.The incidence of inguinal hernia is on the rise,and there may be an association between its pathogenesis and the mechanism of sarcopenia,as well as the adverse effects of sarcopenia on various postoperative conditions.This paper aims to investigate the relationship between the recognition,diagnosis,treatment,pathogenesis,early postoperative clinical recovery and recurrence of sarcopenia in patients with inguinal hernia.

Objective To investigate the clinical diagnostic value of lung ultrasound(LUS)com-bined with serum interleukin(IL)-1β and IL-12p70 for pediatric community-acquired pneumonia(CAP).Methods A total of 136 pediatric patients with suspected CAP were enrolled and divided in-to CAP group(95 patients)and non-CAP group(41 patients)based on chest computed tomography(CT)results as the gold standard.The LUS characteristics,LUS scores,serum levels of IL-1 β and IL-12p70 were compared between the two groups at admission.Receiver operating characteristic(ROC)curves were plotted to analyze the diagnostic efficacy of LUS combined with serum IL-1 β and IL-12p70 for CAP.Results The incidence rates of interstitial disease,pleural line abnormalities,pleural effusion,lung consolidation,and bronchial signs were higher in the CAP group than those in the non-CAP group(P＜0.05).The serum levels of IL-1 β,IL-12p70 and LUS scores were also high-er in the CAP group than in the non-CAP group(P＜0.05).ROC curve analysis showed that the area under the curve for the diagnosis of CAP using LUS scores combined with serum IL-1 β and IL-12p70 at admission was 0.981,with a sensitivity of 93.68％,a specificity of 95.12％,and a diagnostic effica-cy was significantly higher than that of each index alone(P＜0.05).Conclusion LUS combined with serum IL-1 β and IL-12p70 has high diagnostic value for pediatric CAP.