Objective To investigate the expression and prognostic value of heat shock protein B7(HSPB7)in gastric cancer.Methods Immunohistochemistry SP was used to detect HSPB7 and p53 protein expression in 73 cases of gastric cancer and adjacent normal tissues,and their relationships with clinicopatho-logical parameters were analyzed.Spearman's rank correlation analysis was performed to analyze their correla-tion.Kaplan-Meier survival curves and Log-rank tests were used for survival analysis,and a Cox proportional hazards model was applied to analyze prognostic factors.qPCR was used to determine HSPB7 mRNA expres-sion in 22 cases of gastric cancer and normal gastric tissues,and differences between cancer and normal tissues were analyzed.Data from The Cancer Genome Atlas(TCGA)database were used to further analyze HSPB7 mRNA expression differences between gastric cancer and normal tissues and their relationship with clinico-pathological parameters.Results HSPB7 was mainly localized in the cytoplasm,and its expression in gastric cancer tissues was significantly lower than that in adjacent normal tissues(P<0.05),and its expression in gastric cancer tissues was related to TNM stage,tumor invasion depth,differentiation degree and lymph node metastasis(P<0.05).The expression of p53 in gastric cancer was significantly higher than that in adjacent normal tissues(P<0.05),and the expression in gastric cancer tissues was correlated with TNM stage,tumor invasion depth and differentiation degree(P<0.05).Spearman rank correlation analysis showed that the ex-pressions of HSPB7 and p53 were negatively correlated in gastric cancer(r=-0.351,P=0.002).The 5-year survival rate of patients in the positive group was significantly higher than that in the negative group(χ2=10.611,P<0.001).Compared with gastric cancer tissues,the mRNA expression level of HSPB7 in adjacent normal tissues was significantly higher(t=-6.180,P<0.001).The results of bioinformatics analysis showed that the mRNA expression of HSPB7 in normal gastric samples was significantly higher than that in gastric cancer samples(P<0.001),and it was related to age,pathological stage and T stage(P<0.05).Conclusion HSPB7 protein is expressed at low levels in gastric cancer tissues,and it is closely related to the clinicopathological pa-rameters and prognosis of gastric cancer patients,which may be used as a predictive index of gastric cancer malignancy.In gastric cancer tissues,they may have antagonistic effects in tumor progression,and their related signaling pathways need to be further explored.