Objective:To investigate the prevalence of brucellosis patients combined with Mycobacterium tuberculosis( M. tuberculosis) and diagnostic efficiency of different tuberculosis immunological methods in screening brucellosis patients complicated with tuberculosis. Methods:A retrospective study was conducted to collect medical records of hospitalized patients diagnosed with brucellosis in the Department of Infectious Diseases 920th Hospital of Joint Logistics Support Force from January 1, 2020 to December 31, 2023, who had tuberculosis interferon gamma release assay (IGRA) examination records. The incidence of M. tuberculosis co-infection in brucellosis patients with different characteristics, as well as the diagnostic efficiency of different tuberculosis immunological methods were compared. Results:A total of 233 patients with brucellosis were included, and 63 patients with combined with M. tuberculosis infection were detected, with a combined M. tuberculosis infection rate of 27.0%. According to different characteristics, the incidence of M. tuberculosis co-infection in brucellosis patients aged > 45 years (31.9%, 52/163) was significantly higher than that in patients aged ≤45 years (15.7%, 11/70), and the difference was statistically significant (χ 2 = 6.51, P = 0.011). Among different tuberculosis immunological methods, the positive rates of IGRA, tuberculosis antibody and tuberculin skin test (TST) were 21.0% (49/233), 5.3% (12/226), and 4.9% (9/185), respectively, with statistically significant differences among the three groups (χ 2 = 38.92, P < 0.001). Conclusions:The proportion of brucellosis patients combined with M. tuberculosis infection is relatively high, and middle-aged and elderly brucellosis patients are the key population for complicated infections. When screening brucellosis patients combined with M. tuberculosis infection, the diagnostic efficiency of IGRA is significantly better than that of tuberculosis antibody and TST testing.

Objective:To investigate the prevalence of brucellosis patients combined with Mycobacterium tuberculosis( M. tuberculosis) and diagnostic efficiency of different tuberculosis immunological methods in screening brucellosis patients complicated with tuberculosis. Methods:A retrospective study was conducted to collect medical records of hospitalized patients diagnosed with brucellosis in the Department of Infectious Diseases 920th Hospital of Joint Logistics Support Force from January 1, 2020 to December 31, 2023, who had tuberculosis interferon gamma release assay (IGRA) examination records. The incidence of M. tuberculosis co-infection in brucellosis patients with different characteristics, as well as the diagnostic efficiency of different tuberculosis immunological methods were compared. Results:A total of 233 patients with brucellosis were included, and 63 patients with combined with M. tuberculosis infection were detected, with a combined M. tuberculosis infection rate of 27.0%. According to different characteristics, the incidence of M. tuberculosis co-infection in brucellosis patients aged > 45 years (31.9%, 52/163) was significantly higher than that in patients aged ≤45 years (15.7%, 11/70), and the difference was statistically significant (χ 2 = 6.51, P = 0.011). Among different tuberculosis immunological methods, the positive rates of IGRA, tuberculosis antibody and tuberculin skin test (TST) were 21.0% (49/233), 5.3% (12/226), and 4.9% (9/185), respectively, with statistically significant differences among the three groups (χ 2 = 38.92, P < 0.001). Conclusions:The proportion of brucellosis patients combined with M. tuberculosis infection is relatively high, and middle-aged and elderly brucellosis patients are the key population for complicated infections. When screening brucellosis patients combined with M. tuberculosis infection, the diagnostic efficiency of IGRA is significantly better than that of tuberculosis antibody and TST testing.

Objective:To explore the chain mediating effect of anxiety and flow experience on perceived stress and mobile phone addiction in nursing college students.Methods:In December 2021, a cross-sectional design survey was conducted on 4 179 freshmen and sophomores in a nursing college in Heilongjiang Province. The Chinese perceived stress scale, generalized anxiety disorder-7, flow state scale, and mobile phone addiction tendency scale were selected separately to assess perceived stress, anxiety symptoms, flow experience and mobile phone addiction. SPSS 26.0 software was used for descriptive analysis, independent sample t-test, Spearman correlation analysis, and AMOS 24.0 software was used for mediating effect test. Results:(1) Among the 3 050 nursing students, there were 714(23.41%) students who were addicted to mobile phones. (2) Spearman correlation analysis indicated that perceived stress(27.31±9.56) was positively correlated with anxiety(7.00(1.00, 10.00), r=0.441, P<0.05), flow experience((12.00±3.40), r=0.517, P<0.05), and mobile phone addiction((42.42±13.05), r=0.476, P<0.05).Anxiety was positively correlated with flow experience ( r=0.430, P<0.01) and mobile phone addiction ( r=0.538, P<0.01).Flow experience was positively correlated with mobile phone addiction ( r=0.490, P<0.01). (3) Anxiety and flow experience played seperate mediating and chain mediating roles between perceived stress and mobile phone addiction, accounting for 26.06%(0.165/0.633), 23.54%(0.149/0.633) and 3.48%(0.022/0.633) of the total effect. Conclusion:Perceived stress not only directly affects the mobile phone addiction of nursing students, but also indirectly affects mobile phone addiction through the independent and chain mediating effects of anxiety and flow experience.

Objective:To evaluate the clinical effects of problem solving therapy on the elderly patients with depression by Meta-analysis.Methods:Randomized controlled trials articles regarding the effects of problem solving therapy on the elderly patients with depression were searched from the database of PubMed, EMbase, Medline, Cochrane Library, China National Knowledge Infrastructure, Chinese Biomedical Database and Wanfang Data electronically. SMD was used as the post merger response, and its 95% CI was calculated. Results:Ten articles were recruited and a total of 1 005 patients were included, 501 patients in the intervention group and 504 patients in the control group. Meta-analysis showed that problem solving therapy was significantly better than the control group in the treatment of depressive symptoms in the elderly patients with depression ( SMD =-0.64, 95% CI -0.98 - -0.31, P<0.05); during the follow-up within 3 months and 6 months after the intervention, the improvement of depression in the intervention group was better than that in the control group ( SMD=-0.62, 95% CI -1.00 - -0.25, P<0.05) and ( SMD =-0.79, 95% CI -1.03 - -0.55, P<0.05), respectively. Compared with the control group, the intervention group had an effect on the improvement of dysfunction ( SMD=-1.28, 95% CI -2.22 - -0.35, P<0.05). Conclusions:Problem solving therapy can improve the depressive symptoms of the elderly patients with depression and the clinical effect remains stable for a certain period of time; problem solving therapy has an improvement effect on the dysfunction of the elderly patients with depression.

【Objective】 To explore and verify the mechanism of curcumin’s inhibition of the proliferation of renal clear cell carcinoma （RCCC） based on network pharmacology and bioinformatics. 【Methods】 We screened common target genes of RCCC and curcumin from PharmMapper and GeneCards databases. We used TCGA database data analysis to screen out common target genes which not only differentially expressed between RCCC tissue samples and normal tissue samples but also affected prognosis. We also used STRING platform to construct curcumin-RCCC targets interaction network， used R software to perform GO biological process analysis and KEGG pathway enrichment analysis based on the above-mentioned screening target proteins. After curcumin and/or active oxygen inhibitor N-acetyl-L-cysteine （NAC） were incubated in renal cancer 786-O and ACHN cells， CCK8 was used to detect the effects of different concentrations of curcumin on cell proliferation and cell viability. Reactive oxygen detection kit （DCFH-DA） was used to detect the level of intracellular reactive oxygen species， and malondialdehyde （MDA） determination kit （TBA method） to detect intracellular malondialdehyde changes. 【Results】 PharmMapper website and GeneCards database screened out 109 common targets of curcumin and RCCC. TCGA database data analysis screened out 37 differentially expressed genes （DEGs） that might affect the overall survival of patients. The core target proteins of curcumin screened out by protein-protein interaction （PPI） that inhibited the biological behavior of RCCC mainly involved CASP3， EGFR， CHEK1， HSP90AA1， and AR. GO enrichment analysis identified 213 items， mainly including reactive oxygen species metabolic process， response to steroid hormones， fibrinolysis and other biologically active processes. KEGG enrichment analysis identified 24 items， which were mainly related to pyruvate metabolism， glycolysis/gluconeogenesis， FoxO signaling pathway， colorectal cancer， tyrosine metabolism， IL-17 signaling pathway， apoptosis and other signaling pathways. Curcumin reduced the cell viability of 786-O and ACHN in a time- and dose-dependent manner （P<0.05）. After curcumin was incubated with kidney cancer cells， the level of reactive oxygen species and MDA increased significantly （P<0.05）. The addition of NAC reversed the effect of curcumin on the cell viability of 786-O and ACHN cells （P<0.05）. 【Conclusion】 Curcumin may participate in the oxidative stress pathway to inhibit the proliferation of renal cell carcinoma.

Coping style is a cognitive or behavioral strategy taken by individuals in the face of stress. Positive coping style is of great significance for improving the physical and mental outcomes of elderly patients with urinary incontinence. Accurate assessment of coping styles for the elderly patients with urinary incontinence can provide reference for the subsequent development of intervention measures. The existing coping style assessment tools for elderly incontinence at home and abroad include specific scale of incontinence, relevant psychological assessment scale, and universal scale. In a word, the progress in the studies on relevant assessment tools is slow, and it mainly focuses on the assessment of female population. The assessment content is relatively single and lacks of pertinence and systematization. In the future, a comprehensive scale with strong adaptability should be developed based on the characteristics of elderly incontinence patients in China.
Adaptation, Psychological ; Aged ; China ; Female ; Humans ; Urinary Incontinence

Objective: To study the mutual effect of EPHA3 gene polymorphism, folic acid intake in early pregnancy, and the occurrence of non-syndromic cleft lip with or without cleft palate, so as to provide the oretical basis for a etiological study on non-syndromic cleft lip with or without cleft palate. Methods: A total of 195 patients and 170 controls were included.The candidate site rs7650466 was genotyped by Snapshot technique.The correlation with folic acid intake in early pregnancy was analyzed. Result: The rs7650466 T allele was highly associated with the pathogenesis of non-syndromic cleft lip with or without cleft palate(OR=0.2472, 95%CI=0.1567-0.3899, P=1.851×10^-9). The T allele had protective effects.Folic acid intake in early pregnancy, and its interaction with EPHA3 gene, were associated with the pathogenesis of this disease(P<0.05). Conclusions EPHA3 gene, folic acid intake during the first trimester, and theinteractionsare associated with the pathogenesis of non-syndromic cleft lip with or without cleft palate.