Objective: To understand the epidemiological characteristics and disease burden of influenza like illness (ILI) among student populations, so as to provide data support for policy formulation and optimal allocation of health resources. Methods: From January 2024 to February 2025, a questionnaire survey was conducted among parents of kindergarten, primary school, junior and senior high school students in 9 districts of Shenzhen, including Longhua, Futian, Bao an, Longgang, Luohu, Nanshan, Guangming, Pingshan and Yantian. Parents were asked to complete the questionnaire based on whether their children had fever, cough, vomiting, diarrhea, rash and other common symptoms in 2024. A total of 3 537 parents were investigated, and 444 ILI cases were included as study subjects. The epidemiological burden, including incidence rate of influenza, visitation rate, years lived with disability (YLDs) and economic burden (including direct economic burden, indirect economic burden and intangible burden) were analyzed. Results: The incidence rate of influenza among students in Shenzhen in 2024 was 12.55%. The ILI incidence rates in kindergarten, primary school, junior and senior high school were 14.01%, 11.69% and 5.23%, respectively, with a statistically significant difference ( χ 2= 45.20, P <0.01). The ILI consultation rate among students was 85.36%, and the consultation rates in kindergarten (87.36%) and primary school students (84.62%) were higher than those in junior and senior high school students ( 56.52 %) ( χ 2=16.47, P <0.01). A total of 78.88% of cases did not receive etiological detection.The median total economic burden per ILI case was 2 354.62 yuan, including direct medical costs of 300.00 yuan, direct non medical costs of 212.50 yuan, indirect costs of 1 000.00 yuan, and intangible burden of 500.00 yuan. Conclusions Schools are high risk environment for influenza, and younger students are a high risk group for ILI. The disease burden caused by student ILI remains substantial.

Objective:To examine radiological and clinical data to investigate the influence of PVP/PKP surgery on disc degeneration and treatment outcomes in patients with osteoporotic vertebral compression fractures. Additionally, by analyzing clinical and radiological data, we aim to identify the risk factors for postoperative re-fracture in patients with osteoporotic vertebral compression fractures.Methods:This retrospective study collected clinical data from 100 patients who underwent PVP/PKP surgery and conservative treatment for osteoporotic vertebral compression fractures (OVCF) at our hospital from Jan. 2021 to Jan. 2023. Clinical characteristics of the included patients were collected, including demographic data, surgical duration, intraoperative blood loss, and volume of bone cement injected. Furthermore, the visual analog scale (VAS) scores, oswestry disability index (ODI) scores, radiographic measurements, anterior vertebral height, bone mineral density (BMD) T-scores, Cobb angle were collected for all patients during the one-year follow-up period, and to diagnose whether the patient developed a new vertebral fracture postoperatively.Results:Starting from the first month after surgery, the VAS scores and ODI scores of the PVP/PKP group were significantly lower than those of the conventional group ( P<0.05). Additionally, there were no significant differences in BMD T-scores, anterior vertebral height, or Cobb angle at all time points between the two groups of patients. Compared to OVCF patients in the non-re-fracture group, patients in the re-fracture group had significantly older age, significantly lower preoperative BMD T-scores, and significantly higher preoperative Cobb angles. Logistic regression analysis showed that preoperative BMD T-scores and preoperative Cobb angle were risk factors for vertebral re-fracture in OVCF patients within 1 year after surgery. Conclusions:This retrospective study demonstrates that PVP/PKP treatment effectively alleviates clinical symptoms in OVCF patients without increasing the risk of re-fracture. These findings provide new clinical evidence for the treatment of OVCF patients.

Objective:To examine radiological and clinical data to investigate the influence of PVP/PKP surgery on disc degeneration and treatment outcomes in patients with osteoporotic vertebral compression fractures. Additionally, by analyzing clinical and radiological data, we aim to identify the risk factors for postoperative re-fracture in patients with osteoporotic vertebral compression fractures.Methods:This retrospective study collected clinical data from 100 patients who underwent PVP/PKP surgery and conservative treatment for osteoporotic vertebral compression fractures (OVCF) at our hospital from Jan. 2021 to Jan. 2023. Clinical characteristics of the included patients were collected, including demographic data, surgical duration, intraoperative blood loss, and volume of bone cement injected. Furthermore, the visual analog scale (VAS) scores, oswestry disability index (ODI) scores, radiographic measurements, anterior vertebral height, bone mineral density (BMD) T-scores, Cobb angle were collected for all patients during the one-year follow-up period, and to diagnose whether the patient developed a new vertebral fracture postoperatively.Results:Starting from the first month after surgery, the VAS scores and ODI scores of the PVP/PKP group were significantly lower than those of the conventional group ( P<0.05). Additionally, there were no significant differences in BMD T-scores, anterior vertebral height, or Cobb angle at all time points between the two groups of patients. Compared to OVCF patients in the non-re-fracture group, patients in the re-fracture group had significantly older age, significantly lower preoperative BMD T-scores, and significantly higher preoperative Cobb angles. Logistic regression analysis showed that preoperative BMD T-scores and preoperative Cobb angle were risk factors for vertebral re-fracture in OVCF patients within 1 year after surgery. Conclusions:This retrospective study demonstrates that PVP/PKP treatment effectively alleviates clinical symptoms in OVCF patients without increasing the risk of re-fracture. These findings provide new clinical evidence for the treatment of OVCF patients.

Objective:To investigate the effect and potential mechanism of serum exosome-derived cirC_0009362 on the osteogenic differentiation of human bone marrow mesenchymalstem cells (hBMSCs) .Methods:Serum samples from patients with osteoporosis (OP) were collected and exosomes were isolated. The expression level of circ_0009362 in exosomes was detected by qRT-PCR. hBMSCs osteogenesis was induced and the expression of circ_0009362 and miR-29b-3p was detected. The interaction between circ_0009362 and miR-29b-3p was detected by dual luciferase reporter assay. Alkaline phosphatase (ALP) kit was used to detect ALP activity and alizarin red (ARS) staining was used to detect calcium deposition.Results:Compared with healthy control group, the expression of circ_0009362 in serum exosomes of OP patients was increased, and the expression of circ_0009362 was decreased after inducing hBMSCs osteogenesis (all P<0.05) . The ALP activity and the percentage of calcium deposition in hBMSCs were decreased by exosomes, and this effect was achieved by secreting circ_0009362. The effect of exosomes was partially offset by circ_0009362 expression in knockdown exosomes (all P<0.05) . The expression of miR-29b-3p was increased after inducing hBMSCs osteogenesis ( P<0.05) . Circ_0009362 had a targeting relationship with miR-29b-3p, and exosomes inhibited the expression of miR-29b-3p by secreting circ_0009362. The ALP activity and the percentage of calcium deposition in hBMSCs were promoted by overexpression of miR-29b-3p, which was partially offset by exosomes (all P<0.05) . Conclusion:Serum exosomes of OP patients inhibit the osteogenic differentiation of hBMSCs by secreting circ_0009362 to down-regulate the expression of miR-29b-3p.