Objective To investigate the effects of miR-1 on the proliferation,migration and invasion of esophageal squamous cell carcinoma.Methods miR-1 mimics,inhibitors and their corresponding controls were transfected into ESCC cells ECa109 and KYSE410.The efficiency of cell transfection was validated by SYBR-green quantitative PCR.The proliferation,migration and invasion ability of ESCC cells were assessed by MTT,foci formation,wound healing and Matrigel invasion assays,respectively.Statistical analysis was carried out using SPSS 22.0software for Windows.Results Overexpression of miR-1 markedly inhibited the cell growth rate and the colony-forming ability of the ESCC cells.While,downregulation of miR-1 promoted the cell growth rate and foci formation(P＜0.05).And upregulation or downregulation of miR-1 significantly suppressed or promoted tumor cell invasion and migration(P＜0.05).Conclu-sion miR-1 inhibited ESCC cell proliferation,invasion and migration,suggesting that miR-1 functions as a tumor suppressor in the tumorigenesis and progression of ESCC and may be a potential therapeutic target.

Objective To investigate the effects of miR-1 on the proliferation,migration and invasion of esophageal squamous cell carcinoma.Methods miR-1 mimics,inhibitors and their corresponding controls were transfected into ESCC cells ECa109 and KYSE410.The efficiency of cell transfection was validated by SYBR-green quantitative PCR.The proliferation,migration and invasion ability of ESCC cells were assessed by MTT,foci formation,wound healing and Matrigel invasion assays,respectively.Statistical analysis was carried out using SPSS 22.0software for Windows.Results Overexpression of miR-1 markedly inhibited the cell growth rate and the colony-forming ability of the ESCC cells.While,downregulation of miR-1 promoted the cell growth rate and foci formation(P＜0.05).And upregulation or downregulation of miR-1 significantly suppressed or promoted tumor cell invasion and migration(P＜0.05).Conclu-sion miR-1 inhibited ESCC cell proliferation,invasion and migration,suggesting that miR-1 functions as a tumor suppressor in the tumorigenesis and progression of ESCC and may be a potential therapeutic target.

Objective: To investigate the correlation between poorly differentiated clusters (PDCs) in colorectal adenocarcinomas with clinicopathological parameters and its clinicopathological significance. Methods: One hundred and eighty-three colorectal adenocarcinomas resected by radical proctocolecomy were collected at Nanjing Hospital(Nanjing First Hospital), Nanjing Medical University, from January to December 2017. There were 122 male and 61 female patients with age ranging from 42 to 89 years (mean of 68 years). Tumor diameter ranged from 2 to 14 cm (mean 4.5 cm). There were 124 colon cancers and 59 rectal cancers. The number and grade of PDCs in the colorectal adenocarcinoma were evaluated by H-E staining. The overall peritumoural inflammatory reaction was also evaluated. The relationship between PDCs and tumor grades and clinicopathological features and overall peritumoural inflammatory reaction of colorectal adenocarcinoma was analyzed. Results: Of 183 cases of colorectal adenocarcinoma, PDCs were seen in 104 cases (56.8%), of which 36 cases (19.7%) were grade 1, 28 cases (15.3%) were grade 2, and 40 cases (21.9%) were grade 3. PDCs were positively correlated with lymph node metastasis, vascular invasion, degree of differentiation, depth of invasion, and pTNM staging(P<0.05). The detection rate of PDCs in colon cancer was higher than that of rectal cancer(P<0.05). PDCs was unrelated to age, gender, tumor size, and degree of overall peritumoural inflammatory reaction (P>0.05). Among clinicopathological parameters, the grade of PDCs was correlated with lymph node metastasis and vascular invasion (higher than those without lymph node metastasis and vascular invasion, P<0.05); There was a positive correlation between the grade of PDCs and age, tumor differentiation and pTNM staging(P<0.05), and no significant difference between the grade of PDCs and gender, tumor size, tumor location, and depth of invasion was seen (P>0.05). There was no correlation between the grade of PDCs and the degree of overall peritumoural inflammatory reaction (P>0.05). Conclusions PDC is a histological feature that predicts the aggressiveness of colorectal adenocarcinoma. Evaluation of PDC grade can better predict the biological behavior of colorectal cancer and more accurately guide the treatment and evaluate prognosis.

Objective To investigate the correlation between poorly differentiated clusters (PDCs) in colorectal adenocarcinomas with clinicopathological parameters and its clinicopathological significance. Methods One hundred and eighty?three colorectal adenocarcinomas resected by radical proctocolecomy were collected at Nanjing Hospital(Nanjing First Hospital), Nanjing Medical University, from January to December 2017. There were 122 male and 61 female patients with age ranging from 42 to 89 years (mean of 68 years). Tumor diameter ranged from 2 to 14 cm (mean 4.5 cm). There were 124 colon cancers and 59 rectal cancers. The number and grade of PDCs in the colorectal adenocarcinoma were evaluated by H?E staining. The overall peritumoural inflammatory reaction was also evaluated. The relationship between PDCs and tumor grades and clinicopathological features and overall peritumoural inflammatory reaction of colorectal adenocarcinoma was analyzed. Results Of 183 cases of colorectal adenocarcinoma, PDCs were seen in 104 cases (56.8%), of which 36 cases (19.7%) were grade 1, 28 cases (15.3%) were grade 2, and 40 cases (21.9%) were grade 3. PDCs were positively correlated with lymph node metastasis, vascular invasion, degree of differentiation, depth of invasion, and pTNM staging(P<0.05). The detection rate of PDCs in colon cancer was higher than that of rectal cancer(P<0.05). PDCs was unrelated to age, gender, tumor size, and degree of overall peritumoural inflammatory reaction (P>0.05). Among clinicopathological parameters, the grade of PDCs was correlated with lymph node metastasis and vascular invasion (higher than those without lymph node metastasis and vascular invasion, P<0.05); There was a positive correlation between the grade of PDCs and age, tumor differentiation and pTNM staging(P<0.05), and no significant difference between the grade of PDCs and gender, tumor size, tumor location, and depth of invasion was seen (P>0.05). There was no correlation between the grade of PDCs and the degree of overall peritumoural inflammatory reaction (P>0.05). Conclusions PDC is a histological feature that predicts the aggressiveness of colorectal adenocarcinoma. Evaluation of PDC grade can better predict the biological behavior of colorectal cancer and more accurately guide the treatment and evaluate prognosis.