Objective:To preliminarily establish a valuable threshold for the college students’ psychological Suzhi questionnaire simplified version (CSPSz-SV) by means of the latent profile analysis and the receiver operating characteristic(ROC) curve.Methods:The CSPSz-SV was applied to investigate 1 870 college freshmen from undergraduate colleges. The identification threshold value of the CSPSz-SV was identified by combining latent profile analysis (LPA) and the ROC curve.Both Mplus 8.3 and R 4.3.1 software were used for the data analysis.Results:Based on LPA results, the college freshmen were divided into three categories according to the levels of psychological Suzhi: the low psychological Suzhi group, the medium psychological Suzhi group, and the high psychological Suzhi group.Following the LPA, the ROC analysis was conducted to obtain the optimal critical value (91) of the psychological Suzhi level for college freshmen (Youden's index was 0.934, sensitivity was 0.979, specificity was 0.955, positive predictive value was 0.855, negative predictive value was 0.994, accuracy was 0.960, and AUC was 0.991, respectively). College freshmen were divided into the abnormal psychological Suzhi group and the normal psychological Suzhi group in accordance with this critical value, and the difference between the two groups was statistically significant (Cohen's d=1.74-2.18, P<0.001). Conclusion:The threshold value established and obtained in this study can be reviewed as a reference for evaluating the psychological Suzhi level of college freshmen.

Objective:To preliminarily establish a valuable threshold for the college students’ psychological Suzhi questionnaire simplified version (CSPSz-SV) by means of the latent profile analysis and the receiver operating characteristic(ROC) curve.Methods:The CSPSz-SV was applied to investigate 1 870 college freshmen from undergraduate colleges. The identification threshold value of the CSPSz-SV was identified by combining latent profile analysis (LPA) and the ROC curve.Both Mplus 8.3 and R 4.3.1 software were used for the data analysis.Results:Based on LPA results, the college freshmen were divided into three categories according to the levels of psychological Suzhi: the low psychological Suzhi group, the medium psychological Suzhi group, and the high psychological Suzhi group.Following the LPA, the ROC analysis was conducted to obtain the optimal critical value (91) of the psychological Suzhi level for college freshmen (Youden's index was 0.934, sensitivity was 0.979, specificity was 0.955, positive predictive value was 0.855, negative predictive value was 0.994, accuracy was 0.960, and AUC was 0.991, respectively). College freshmen were divided into the abnormal psychological Suzhi group and the normal psychological Suzhi group in accordance with this critical value, and the difference between the two groups was statistically significant (Cohen's d=1.74-2.18, P<0.001). Conclusion:The threshold value established and obtained in this study can be reviewed as a reference for evaluating the psychological Suzhi level of college freshmen.

Objective To systematically evaluate the clinical efficacy of Chinese Medicine Compound(CHM)for menopause syndrome.Methods China National Knowledge Infrastructure(CNKI),China Science and Technology Journal Database(VIP)and Wanfang full-text database were searched for a randomized controlled trial(RCT)related to the efficacy of CHM therapy published from January 1993 to May 2023.Literature quality was evaluated according to the Cochrane manual and the Meta-analysis was performed using RevMan 5.4 software.Results A total of 131 RCTs were included in this study,and Meta-analysis showed that CHM improved clinical response rate(OR=3.90,95％CI:3.50-4.34,P＜0.00001),reduced Kupperman score(MD=-3.69,95％9∶-4.79--2.58,P＜0.000 01)and decreased LH level(MD=-2.79,95％CI:-4.65--0.92,P=0.003).The results of subgroup analysis showed that the clinical efficacy of menopausal patients treated by Gui-Zhi-Long-Gu-Mu-Li decoction,Dan-Zhi-Xiao-Yao-San,Er-Xian decoction,Liu-Wei-Di-Huang decoction,Zhi-Bai-Di-Huang decoction and Zi-Shui-Qing-Gan decoction was better than that of hormone therapy(OR=3.83,95％CI:3.13-4.68,P=0.002).Conclusion CHM treatment can significantly improve clinical efficiency,relieve discomfort symptoms,reduce Kupperman score and improve LH level.

Purpose To investigate the expression status of GNL3 in gastric cancer,and to explore the role of GNL3 in tumor proliferation,invasion and migration.Methods The ex-pression of GNL3 mRNA in gastric cancer tissues was analyzed by searching database.The expression of GNL3 protein in 51 gastric cancer tissues and 51 adjacent non-tumor tissues was de-tected by immunohistochemistry(IHC)SP method.The correla-tion between GNL3 protein expression and gastric cancer clinical pathological features was analyzed by x2 test.The expression of GNL3 in gastric cancer cells was silenced by transfection of sh-RNA,and the silencing efficiency was verified by Western blot.The effect of silencing GNL3 on the proliferation of gastric cancer cells was examined by CCK-8,colony formation and EdU stai-ning.In addition,wound-healing assay and Transwell assay were performed to detect the effect of GNL3 silencing on cell invasion and migration.Results SangerBox and UALCAN database re-trieval showed that the expression of GNL3 mRNA was signifi-cantly increased in gastric cancer tissues(P＜0.01).IHC stai-ning showed that the positive expression rate of GNL3 protein in gastric cancer tissues was 96.1％,and the high expression rate was 78.4％,which was significantly higher than that in adjacent non-tumor tissues(74.5％,51.0％,P＜0.01).Moreover,the high expression of GNL3 was significantly correlated with lymph node metastasis in gastric cancer patients(x2=4.933,P=0.026).CCK-8,colony formation and EdU staining showed that GNL3 silencing inhibited the proliferation of gastric cancer cell SGC-7901.The wound-healing and Transwell assay showed that GNL3 silencing inhibited the migration and invasion of gastric cancer cell.Conclusion The GNL3 protein is highly expressed in gastric cancer tissues,and closely related to the proliferation,migration and invasion of gastric cancer cells.

Objective To systematically evaluate the clinical efficacy of Chinese Medicine Compound(CHM)for menopause syndrome.Methods China National Knowledge Infrastructure(CNKI),China Science and Technology Journal Database(VIP)and Wanfang full-text database were searched for a randomized controlled trial(RCT)related to the efficacy of CHM therapy published from January 1993 to May 2023.Literature quality was evaluated according to the Cochrane manual and the Meta-analysis was performed using RevMan 5.4 software.Results A total of 131 RCTs were included in this study,and Meta-analysis showed that CHM improved clinical response rate(OR=3.90,95％CI:3.50-4.34,P＜0.00001),reduced Kupperman score(MD=-3.69,95％9∶-4.79--2.58,P＜0.000 01)and decreased LH level(MD=-2.79,95％CI:-4.65--0.92,P=0.003).The results of subgroup analysis showed that the clinical efficacy of menopausal patients treated by Gui-Zhi-Long-Gu-Mu-Li decoction,Dan-Zhi-Xiao-Yao-San,Er-Xian decoction,Liu-Wei-Di-Huang decoction,Zhi-Bai-Di-Huang decoction and Zi-Shui-Qing-Gan decoction was better than that of hormone therapy(OR=3.83,95％CI:3.13-4.68,P=0.002).Conclusion CHM treatment can significantly improve clinical efficiency,relieve discomfort symptoms,reduce Kupperman score and improve LH level.

Objective To explore the improvements of high-fat intake on lung injury induced by Paragonimus proliferus infection in rats, and to preliminarily explore the mechanisms underlying the role of cytochrome P450 4A1 (CYP 4A1) in the improve ments. Methods SD rats were randomly assigned into three groups, including the normal control group (n = 10), the infection and normal diet group (n = 12) and the infection and high-fat diet group (n = 12). Rats in the normal control group were fed with normal diet and without any other treatments, and animals in the infection and normal diet group were subcutaneously injected with 8 excysted metacercariae of P. proliferus via the abdominal wall, followed by feeding with normal diet, while rats in the infection and high-fat diet group were subcutaneously injected with 8 excysted metacercariae of P. proliferus via the abdominal wall, followed by feeding with high-fat diet. All rats were sacrificed 28 weeks post-infection, and serum samples and lung specimens were collected. Following hematoxylin-eosin (HE) staining of rat lung specimens, the rat lung injury was observed under an optical microscope, and alveolitis was evaluated using semi-quantitative scoring. Serum interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF-α) levels were measured using enzyme-linked immunosorbent assay (ELISA), and the cytochrome P450 4A1 (CYP 4A1) expression was quantified in rat lung specimens at transcriptional and translational levels using quantitative real-time PCR (qPCR) and Western blotting assays. Results Alveolar wall thickening, edema and inflammatory cell infiltration were alleviated 28 weeks post-infection with P. proliferus in rats in the infection and high-fat diet group relative to the infection and normal diet group, and no alveolar consolidation was seen in the infection and high-fat diet group. The semi-quantitative score of alveolitis was significantly higher in the infection and normal diet group [(2.200 ± 0.289) points] than in the normal control group [(0.300 ± 0.083) points] and the infection and high-fat diet group [(1.300 ± 0.475) points] (both P values < 0.05), and higher serum IL-1β [(151.586 ± 20.492)] pg/mL and TNF-α levels [(180.207 ± 23.379) pg/mL] were detected in the infection and normal diet group than in the normal control group [IL-1β: (103.226 ± 3.366) pg/mL; TNF-α: (144.807 ± 1.348) pg/mL] and the infection and high-fat diet group [IL-1β: (110.131 ± 12.946) pg/mL; TNF-α: (131.764 ± 27.831) pg/mL] (all P values < 0.05). In addition, lower CYP 4A1 mRNA (3.00 ± 0.81) and protein expression (0.40 ± 0.02) was quantified in lung specimens in the infection and normal diet group than in the normal control group [(5.03 ± 2.05) and (0.84 ± 0.14)] and the infection and high-fat diet group [(11.19 ± 3.51) and (0.68 ± 0.18)] (all P values < 0.05). Conclusion High-fat intake may alleviate lung injuries caused by P. proliferus infection in rats through up-regulating CYP 4A1 expression in lung tissues at both translational and transcriptional levels.

Objective:To investigate the value of minimal residual disease (MRD) in prediction of prognosis in acute lymphoblastic leukemia (ALL) patients with or above complete remission 2 (CR2) underwent.Methods:A retrospective analysis was performed on 201 ALL patients who received allogeneic stem cell transplantation (allo-SCT) and pretransplant disease status ≥CR2 in Peking University People′s Hospital from January 2009 to December 2018. MRD was measured by multi-parameter flow cytometry at 1 month before transplantation and 1 month, 2 months, 3 months, 4 months, 6 months, 9 months or 12 months after transplantation. To investigate the influence of dynamic changes of MRD before and after transplantation on prognosis.Results:201 ALL patients, including 126 males and 75 females, with a median age of 18 years. The 3-year cumulative incidence of relapse (CIR), non-relapse mortality (NRM), leukemia-free survival (LFS) and overall survival (OS) of all cases were 34%, 16%, 50%, and 56%, respectively. Positive pre-SCT MRD patients with higher 3-year CIR (47% vs 26%, P=0.003), lower 3-year LFS (40% vs 55%, P=0.047) and OS (42% vs 60%, P=0.065) than those with negative one. Subjects with positive post-MRD had higher 3-year CIR (73% vs 22%, P<0.001) and lower 3-year LFS (28% vs 56%, P=0.005) and OS (32% vs 60%, P=0.040) compared with those with negative one. Multivariate analysis showed that both pre-MRD and post-MRD were associated with higher CIR ( HR=1.823, P=0.018; HR=3.474, P<0.001), lower LFS ( HR=1.779, P=0.007; HR=2.185, P=0.001) and OS ( HR=1.609, P=0.034; HR=1.970, P=0.001). Negative pre-and post-SCT MRD group had lower 3-year CIR (17%, 42%, 82%; P<0.001) and higher 3-year LFS (61%, 44%, 18%; P<0.001) and OS (63%, 47%, 27%; P<0.001) compared with those unrisen post-SCT MRD group, and increased post-SCT MRD group. Multivariate analysis showed that pre-and post-SCT MRD dynamics were associated with CIR, LFS and OS ( P<0.01 for all) independently. The pre-and post-SCT MRD dynamics could better distinguish CIR (C=0.669) from that of pre-SCT MRD (C=0.587) and post-SCT MRD (C=0.629). Conclusion:Our data suggest that pre-SCT MRD, post-SCT MRD and the dynamic peri-SCT MRD could be used to predict transplant outcome of ALLpatients with or above CR2 who underwent allo-SCT.

Objective:To explore the relationship between anti-human leukocyte antigen (HLA) antibodies and transplant outcomes in patients with hematological diseases who underwent matched sibling donor transplantation (MSDT).Methods:A retrospective analysis was conducted in 168 patients with hematological diseases who received MSDT in Peking University People's Hospital from March 2015 to November 2017. All patients received detection of anti-HLA antibodies before transplantation, and the correlation between anti-HLA antibodies and transplant outcomes such as hematopoietic cells implantation, blood product transfusion and prognosis after transplantation were analyzed.Results:Among the 168 patients, 28 (16.7%) were positive for anti-HLA class Ⅰ or class Ⅱ antibodies, and 14 (8.3%) were positive for both anti-HLA class Ⅰ and class Ⅱ antibodies. All patients received neutrophil engraftment, 164 patients (97.9%) received platelet engraftment. Univariate analysis showed that there were no effects of anti-HLA antibodies on neutrophil engraftment and engraftment time, platelet engraftment and engraftment time, the volume of red cell transfusion, the volume of platelet transfusion, overall survival (OS) rate, disease free survival (DFS) rate and transplant-related mortality (TRM) in patients with hematological diseases underwent MSDT (all P > 0.05). Multivariate analysis showed that platelet engraftment was associated with better OS ( HR=0.065, 95% CI 0.017-0.252, P < 0.01), better DFS ( HR=0.083, 95% CI 0.024-0.289, P < 0.01) and lower TRM ( HR=0.094, 95% CI 0.014-0.626, P=0.015). Conclusion:Anti-HLA antibodies have no effect on transplant outcomes of patients with hematological diseases who have received MSDT.

Hematologic neoplasm relapse remains one of the major causes of death after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Relaspe prediction, prophylaxis and treatment after allo-HSCT are the key issues for improving the therapeutic effects and prognosis. This paper chooses some representative reports from 60th American Society of Hematology (ASH) Annual Meeting to discuss the progress of allo-HSCT.

Objective To investigate the effects of three-level comprehensive interventionof family, community and society on physical and mental health of empty nest disabled elderly. Methods From February 2017 to July 2017, the empty nest elderly aged more than 75 years old and daily life ability scale score ≥14 from two communities under 10 community health service centers directly subordinate toTangshanWorker's Hospital and Hongci Hospital were randomly selected, thenthey were divided into theintervention group andthe control group with 50 persons each. The control group was given blank control, and the intervention group was given a 6-month intervention based on the theory of healthy ecology. The intervention consisted of threes-level of family, community and society, each aspect included four aspects such as daily life, environmental safety, medical care and spiritual comfort. Six months later, the Health Promotion Lifestyle Scale, the Comprehensive Frailty Assessment Instrument, Loneliness Scale, Perceived Social Support from Family Scale, Social Support Rating Scale, Activityof Daily Living Scalewere used to evaluate. Results Sixmonths later, the scores of health promotion behavior (114.44± 6.14), family support (9.16 ± 0.93) and social support (30.06 ± 1.48) in the intervention group were higher than those in the control group(t=45.363, 21.672, 64.046, P<0.01); the scores of frailty(35.96 ± 3.71), loneliness (34.94 ± 4.13) and ADL (20.88 ± 1.49) in the intervention group were lower than those before intervention (t=28.946,-31.295,-18.886, P<0.01). Conclusions Thecomprehensive intervention in three-level of family, community and society based on the theory of ecology health can improve the support of empty nest disabled elderly, reduce loneliness and weakness, improve the health promotion behavior and daily life ability of empty nest elderly, and promote physical and mental health.