Objective To analyze the differences in structural integrity of cerebral white matter fiber bundles in vegetative state(VS)patients with different prognoses,and to construct an early-stage prognostic prediction model for 1-year post-stabilization prognosis.Methods A retrospective analysis was conducted on 52 VS patients admitted to the Department of Rehabilitation Medicine at Zhujiang Hospital of Southern Medical University.Patients were stratified into good prognosis(n=22)and poor prognosis(n=30)at 1-year follow-up based on Coma Recovery Scale-Revised(CRS-R)scores.The fractional anisotropy values of cerebral white matter fiber bundles were derived from diffusion tensor imaging,and for the first time,the scores of the visual subscales of CRS-R were combined with FA values as input features for the prognostic model.To optimize model construction,the least absolute shrinkage and selection operator regression was employed for feature screening,and synthetic minority over-sampling technique for data balancing.The prognostic prediction model was subsequently developed using a support vector machine algorithm and validated via leave-one-out cross-validation.Model performance was evaluated using area under receiver operating characteristic curve,along with accuracy,sensitivity,specificity,and F1 score metrics.Results Following LASSO regression feature screening,the pontine crossing tract,medial lemniscus,tapetum,splenium of corpus callosum,and visual subscale scores were identified as key predictors.A multimodal SVM-based prediction model constructed with the above features could effectively predict the 1-year prognosis of VS patients,achieving a high predictive performance(AUC=0.894).Conclusion The SVM-based model integrating FA values of specific white matter fiber bundles and visual subscale scores demonstrates excellent predictive performance in predicting the 1-year prognosis of VS patients.

Objective:To evaluate the current situation of the protective effect of hearing protection device (HPD) worn by manufacturing workers and discuss their possible influencing factors.Methods:A total of 3634 noisy workers were surveyed and tested. The study conducted surveys of workers on the use of HPD. The 3M TM E-A-R Fit TM binaural verification system was used to measure the personal attenuation device (PAR) of workers wearing HPD. Results:The M ( Q1, Q3) of baseline PAR obtained by 3634 workers was 12 (2, 19) dB. There was a statistically significant difference in baseline PAR among the three types of HPDs ( H=336.39, P<0.01) . After pairwise comparison, it was found that the baseline PAR of workers wearing foam earplugs and earmuffs was higher than that of pre-molded earplugs ( P<0.01) . There were differences in baseline PAR among workers in different industries ( Z=359.73, P<0.01) . Education level, age of using HPD, types of HPDs, noise exposure intensity, with or without knowledge of correct methods, and comfort evaluation were the main factors affecting baseline PAR ( P<0.05) . There were 1536 workers (43.4%) failed the baseline PAR test. After the intervention, the median PAR increased significantly from 1 (0, 6) dB (baseline) to 18 (14, 22) dB (after the intervention) ( P<0.01) . The follow-up test found that the follow-up PAR of 328 workers was higher than the baseline PAR of the initial test, and the follow-up PAR was higher than the post-intervention PAR of the initial test ( P<0.01) . Conclusion:Under the conditions of this study, the protective effect of HPD was affected by factors such as incorrect understanding of wearing methods, exposure to high-intensity noise, low education level, a short period of time of HPD use and low comfort of hearing protectors. The protective effect could be improved through training, optimizing the wearing of hearing protector models, and follow-up interventions. Enterprises should use suitability verification to ensure the correct selection and wearing of noisy workers.

ObjectiveTo optimize the processing technology of honey bran-fried Rosae Laevigatae Fructus（h-RLF）， formulate relevant quality standards， and explore its improving effect and mechanism on mice with ulcerative colitis（UC） induced by dextran sodium sulfate（DSS）. MethodsTaking the content of polysaccharides and water-soluble extract as the indexes， L₉（3⁴） orthogonal test was used to optimize parameters of the amount of honey bran， frying time and frying temperature. The quality of 15 batches of h-RLF decoction pieces was evaluated according to the optimized process， and the inspection limit standard was preliminarily drawn up. Eighty SPF male Kunming mice were randomly divided into 8 groups， including the blank group， model group， mesalazine group（0.13 g·kg^-1）， RLF group（3.77 g·kg^-1）， bran-fried RLF group（3.77 g·kg^-1）， h-RLF low， medium and high dose groups（1.89， 3.77， 7.54 g·kg^-1）， with 10 mice in each group. The mice in the blank group were free to drink pure water， and the other groups were free to drink 3% DSS solution for 7 days to prepare UC mouse model. Each treatment group was given corresponding drugs by intragastric administration， and the blank and model groups were given equal volume of normal saline. The body weight of mice was recorded daily and the disease activity index（DAI） was calculated. After the administration， the colon tissues of mice were collected to observe the pathological changes by hematoxylin-eosin（HE） staining. The levels of tumor necrosis factor（TNF）-α， interleukin（IL）-1β， IL-6 and IL-10 in the colon of mice were detected by enzyme-linked immunosorbent assay（ELISA）. Western blot was used to detect the expression levels of phosphorylation nuclear transcription factor-κB p65（p-NF-κB p65）， Toll-like receptor 4（TLR4）， p-p38 mitogen-activated protein kinase（p-p38 MAPK）， p-extracellular signal-regulated kinase（p-ERK） and p-c-Jun N-terminal kinase（p-JNK） proteins in colon tissues. ResultsThe optimum processing technology of h-RLF was 20 g honey bran per 100 g RLF， and stir-frying at 200 ℃ for 8 min. The limit standard under the examination of h-RLF was preliminarily formulated as follows：the polysaccharide content should not be less than 25% based on anhydrous glucose（C₆H₁₂O₆）， the content of water-soluble extract should not be less than 38%， the moisture content should not be more than 12.0%， the total ash content should not be more than 5.0%， and the acid-insoluble ash content should not be more than 1.0%. The cluster heat map analysis showed that the quality of RLF from Huanggang， Hubei province was better. Animal experiments showed that compared with the blank group， the DAI score of the model group was significantly increased， the levels of TNF-α， IL-1β and IL-6 in the colon tissue were significantly increased， the IL-10 level was significantly decreased， the colonic mucosa was seriously damaged， accompanied by a large number of inflammatory cell infiltration， tissue congestion and a significant reduction in glands， and the expression levels of p-NF-κB p65， TLR4， p-p38 MAPK， p-ERK and p-JNK proteins were significantly increased（P<0.01）. Compared with the model group， each administration group could alleviate the symptoms of colonic ulcer， the structure of colonic crypt was basically intact， and the glands were arranged in an orderly manner. Among them， the high-dose group of h-RLF had a better effect， which could significantly reduce the DAI score and the levels of TNF-α， IL-1β and IL-6 in colon tissue（P<0.01）， and significantly increase the level of IL-10（P<0.01）， alleviate the colonic mucosal injury， and effectively inhibit the expression levels of p-NF-κB p65， TLR4， p-p38 MAPK， p-ERK and p-JNK proteins（P<0.01）. ConclusionThe key parameters of the processing technology of h-RLF are determined， and the optimized technology is stable and feasible. The established quality standard is simple and reliable， and can be used for the quality control. h-RLF can effectively alleviate DSS-induced UC， and its mechanism may be related to inhibiting the activation of NF-κB/TLR4/MAPK pathway.

Bisphenols (BPs) are extensively used in food packaging, personal care products, and plastics, making them prevalent in both living and working environments, which has raised significant concern. As endocrine-disrupting chemicals, BPs exert toxic effects on the female reproductive system by binding to estrogen receptors, thereby activating or inhibiting the expression of genes related to reproductive functions, which disrupts the normal function of the endocrine system. This paper reviewed the effects of bisphenol A (BPA), bisphenol S (BPS), and bisphenol F (BPF) on female reproductive function, focusing on three key aspects: the effects on the female reproductive organs, the occurrence of associated reproductive disorders, and the mechanisms of toxicity. Specifically, this review highlighted the effects on ovarian function, uterine morphology and function, and fallopian tube function, as well as their correlation with polycystic ovary syndrome, endometriosis, miscarriage, and eclampsia. Additionally, the toxic mechanisms of BPs exposure were summarized, providing a scientific basis for future research on the impact of BPs on the female reproductive system, as well as for the assessment of potential health risks and the development of preventive measures.

Objective:To evaluate the current situation of the protective effect of hearing protection device (HPD) worn by manufacturing workers and discuss their possible influencing factors.Methods:A total of 3634 noisy workers were surveyed and tested. The study conducted surveys of workers on the use of HPD. The 3M TM E-A-R Fit TM binaural verification system was used to measure the personal attenuation device (PAR) of workers wearing HPD. Results:The M ( Q1, Q3) of baseline PAR obtained by 3634 workers was 12 (2, 19) dB. There was a statistically significant difference in baseline PAR among the three types of HPDs ( H=336.39, P<0.01) . After pairwise comparison, it was found that the baseline PAR of workers wearing foam earplugs and earmuffs was higher than that of pre-molded earplugs ( P<0.01) . There were differences in baseline PAR among workers in different industries ( Z=359.73, P<0.01) . Education level, age of using HPD, types of HPDs, noise exposure intensity, with or without knowledge of correct methods, and comfort evaluation were the main factors affecting baseline PAR ( P<0.05) . There were 1536 workers (43.4%) failed the baseline PAR test. After the intervention, the median PAR increased significantly from 1 (0, 6) dB (baseline) to 18 (14, 22) dB (after the intervention) ( P<0.01) . The follow-up test found that the follow-up PAR of 328 workers was higher than the baseline PAR of the initial test, and the follow-up PAR was higher than the post-intervention PAR of the initial test ( P<0.01) . Conclusion:Under the conditions of this study, the protective effect of HPD was affected by factors such as incorrect understanding of wearing methods, exposure to high-intensity noise, low education level, a short period of time of HPD use and low comfort of hearing protectors. The protective effect could be improved through training, optimizing the wearing of hearing protector models, and follow-up interventions. Enterprises should use suitability verification to ensure the correct selection and wearing of noisy workers.

Objective:To investigate the differences in brain age and brain age gap (BAG) between patients with prolonged disorders of consciousness (pDoC) and healthy controls (HC).Methods:A retrospective cross-sectional study was performed; 43 patients with pDoC admitted to Rehabilitation Medicine Center, Zhujiang Hospital, Southern Medical University from January 2020 to October 2022 were enrolled; 26 healthy volunteers recruited at the same time and 187 healthy subjects from the publicly available healthy control dataset Nathan Kline Institute-Rockland Sample (NKI-RS) were chosen as HC group. The clinical and imaging data of these subjects were collected. A brain age estimation model was constructed based on amplitude of low-frequency fluctuation (ALFF) in resting-state functional magnetic resonance imaging (rs-fMRI) for healthy individuals, and the pDoC group was used as the test set. A two-sample t-test was used to compare the brain age and BAG differences between the pDoC group and HC group. Pearson correlation analysis was used to explore the correlation between BAG and coma recovery scale-revised (CRS-R) in the pDoC group. Results:The chronological age and estimated brain age in the HC group were (41.54±9.61) and (42.32±10.65) years, respectively, without significant difference ( t=-0.254, P=0.801). The chronological age and estimated brain age in the pDoC group were (49.91±12.03) and (62.79±15.00) years, respectively, with significant difference ( t=-4.341, P<0.001). The BAG in the HC and pDoC groups were (0.78±4.59) and (12.88±7.17) years, respectively, with significant difference ( t=-7.822, P<0.001). Correlation analysis results showed that in the pDoC patients, no correlation was noted between BAG and CRS-R score on the day of imaging scan or 6 months after the day of imaging scan ( r=0.090, P=0.738; r=0.205, P=0.674); no correlation was noted between BAG and difference in CRS-R score (difference value of CRS-R score 6 months after the day of imaging scan-CRS-R score on the day of imaging scan, r=0.246, P=0.687). Conclusion:Compared with the HC subjects, patients with pDoC exhibit an abnormal increase in brain age, suggesting that pDoC caused by severe brain injury may lead to accelerated brain aging.

Objective To analyze the differences in structural integrity of cerebral white matter fiber bundles in vegetative state(VS)patients with different prognoses,and to construct an early-stage prognostic prediction model for 1-year post-stabilization prognosis.Methods A retrospective analysis was conducted on 52 VS patients admitted to the Department of Rehabilitation Medicine at Zhujiang Hospital of Southern Medical University.Patients were stratified into good prognosis(n=22)and poor prognosis(n=30)at 1-year follow-up based on Coma Recovery Scale-Revised(CRS-R)scores.The fractional anisotropy values of cerebral white matter fiber bundles were derived from diffusion tensor imaging,and for the first time,the scores of the visual subscales of CRS-R were combined with FA values as input features for the prognostic model.To optimize model construction,the least absolute shrinkage and selection operator regression was employed for feature screening,and synthetic minority over-sampling technique for data balancing.The prognostic prediction model was subsequently developed using a support vector machine algorithm and validated via leave-one-out cross-validation.Model performance was evaluated using area under receiver operating characteristic curve,along with accuracy,sensitivity,specificity,and F1 score metrics.Results Following LASSO regression feature screening,the pontine crossing tract,medial lemniscus,tapetum,splenium of corpus callosum,and visual subscale scores were identified as key predictors.A multimodal SVM-based prediction model constructed with the above features could effectively predict the 1-year prognosis of VS patients,achieving a high predictive performance(AUC=0.894).Conclusion The SVM-based model integrating FA values of specific white matter fiber bundles and visual subscale scores demonstrates excellent predictive performance in predicting the 1-year prognosis of VS patients.

Objective:To investigate the differences in brain age and brain age gap (BAG) between patients with prolonged disorders of consciousness (pDoC) and healthy controls (HC).Methods:A retrospective cross-sectional study was performed; 43 patients with pDoC admitted to Rehabilitation Medicine Center, Zhujiang Hospital, Southern Medical University from January 2020 to October 2022 were enrolled; 26 healthy volunteers recruited at the same time and 187 healthy subjects from the publicly available healthy control dataset Nathan Kline Institute-Rockland Sample (NKI-RS) were chosen as HC group. The clinical and imaging data of these subjects were collected. A brain age estimation model was constructed based on amplitude of low-frequency fluctuation (ALFF) in resting-state functional magnetic resonance imaging (rs-fMRI) for healthy individuals, and the pDoC group was used as the test set. A two-sample t-test was used to compare the brain age and BAG differences between the pDoC group and HC group. Pearson correlation analysis was used to explore the correlation between BAG and coma recovery scale-revised (CRS-R) in the pDoC group. Results:The chronological age and estimated brain age in the HC group were (41.54±9.61) and (42.32±10.65) years, respectively, without significant difference ( t=-0.254, P=0.801). The chronological age and estimated brain age in the pDoC group were (49.91±12.03) and (62.79±15.00) years, respectively, with significant difference ( t=-4.341, P<0.001). The BAG in the HC and pDoC groups were (0.78±4.59) and (12.88±7.17) years, respectively, with significant difference ( t=-7.822, P<0.001). Correlation analysis results showed that in the pDoC patients, no correlation was noted between BAG and CRS-R score on the day of imaging scan or 6 months after the day of imaging scan ( r=0.090, P=0.738; r=0.205, P=0.674); no correlation was noted between BAG and difference in CRS-R score (difference value of CRS-R score 6 months after the day of imaging scan-CRS-R score on the day of imaging scan, r=0.246, P=0.687). Conclusion:Compared with the HC subjects, patients with pDoC exhibit an abnormal increase in brain age, suggesting that pDoC caused by severe brain injury may lead to accelerated brain aging.

Fermentum Rubrum（FR） is a kind of traditional Chinese medicine with dual-use functions of medicine and food， which has been used for more than 1 000 years. By referring to the ancient herbal classics and modern laws and regulations， the author sorted out the origin of FR， sorted out the development of processing， and analyzed the problems existing in the quality standard， aiming to provide a basis for further research and development of FR. There are many theories about the origin of FR. After summarizing and sorting out the relevant literature， three viewpoints are mainly drawn， including Han dynasty origin theory， Wei and Jin origin theory and Tang dynasty origin theory. After synthesizing the views of various schools， it is believed that the origin of FR should be no later than the Eastern Han dynasty. FR is made from rice by fermentation， which has the effect of strengthening the spleen， eliminating food， promoting blood circulation and removing stasis after fermentation. Although the natural fermentation in ancient times has been able to pay attention to the influence of temperature， humidity and strain on the quality of FR， due to the low level of science and technology， there are still problems such as complicated and cumbersome process， large workload and high labor cost. To the pure fermentation in modern times， the fermentation processing method of FR has been evolved， gradually improved and perfected. However， due to the lack of standardized research， there is no unified standard for the fermentation process of FR in various regions， and the quality standard lags behind seriously， which leads to the unstable product quality on the market. Among the 15 specifications for the preparation of FR， only 6 have been published in the past 5 years， and most of them have not been revised in a timely manner， and some of them have not been included in the updated version. Based on this， it is recommended to carry out a systematic study on processing technology of FR， and the best process is selected to accelerate the revision and improvement of its standardization， so as to improve the quality of FR sold in the market and promote its stable and sustainable development.

[Objective]To identify the relationship between tumor tissue interleukins(ILs)and non-small cell lung cancer(NSCLC)patients with poor response to immune checkpoint blockade(ICB)therapy,and to investigate the differ-ential expression of ILs in tumor of NSCLC patients as well as its effect on ICB response and prognosis.[Methods]A total of 61 patients diagnosed with NSCLC and treated with ICB were retrospectively collected from the data of a previous study.We obtained transcriptome sequencing data from tumor tissues and survival data of the patients before ICB treatment.Us-ing bioinformatics methods,we screened for ILs that significantly affected the efficacy and prognosis of ICB treatment.We evaluated the efficacy of ICB treatment using progressive-free survival(PFS)and assessed the prognosis using overall sur-vival(OS).The Kaplan-Meier survival curve and ROC curve were used to analyze the predictive effect and efficacy of ILs on the efficacy and prognosis of ICB in NSCLC patients.[Results]The results of the univariate Cox regression analysis in our study showed that nine ILs were found to be associated with OS of NSCLC patients treated with ICB at a significance level of P<0.1.Further multivariate analysis revealed that high expression of IL-11,IL-17D,and IL-36A was significant-ly associated with poor prognosis in these patients(P<0.05).The results from the Kaplan-Meier survival curve analysis revealed a significant negative correlation between the high expression of IL-17D and both PFS and OS in NSCLC patients.Specifically,patients with IL-17D high expression had a median PFS of 3.1 months compared with 6.5 months in low ex-pression patients[95%confidence interval(CI)(1.178,3.655),P=0.009].Similarly,the median OS was 9.8 months in the high expression group versus 21.8 months in the low expression group[95%CI(1.116,4.392),P=0.018].ROC curve showed that the prediction performance was favorable[AUCPFS=0.702,95%CI(0.562,0.842),P=0.027;AU-COS=0.684,95%CI(0.550,0.818),P=0.014].Although IL-11 and IL-36A alone were not significant predictors of PFS and OS in NSCLC patients,the median PFS and OS were notably shortened to 2.2 months(P=0.003)and 3.0 months(P<0.001),respectively,when high expression of IL-11 and IL-36A was combined with high expression of IL-17D.The ROC curve analysis demonstrated an improvement in prediction efficiency for both PFS and OS in NSCLC patients[AUCPFS=0.748,95%CI(0.615,0.880),P=0.007;AUCOS=0.703,95%CI(0.573,0.833),P=0.007].[Conclu-sion]The results suggest that high expression of IL-11,IL-17D,and IL-36A is associated with a higher risk of disease progression which correlates to poor PFS and OS in NSCLC patients.