Sarcopenia is a syndrome with clinical manifestations of gradual decline in muscle mass and function. It mostly occurs in the elderly population, which can lead to weight loss and muscle strength weakening, resulting in difficulties in daily activities and seriously affecting the quality of life of patients. In recent years, the relationship between sarcopenia and cardiovascular metabolic diseases has received extensive attention. Studies have shown that sarcopenia is closely related to cardiovascular metabolic diseases such as hypertension, coronary heart disease and diabetes mellitus, and interacts with each other through insulin resistance, endothelial dysfunction, inflammation and other mechanisms. This paper reviews the research progress on sarcopenia and cardiovascular metabolic diseases in the elderly in recent years, focusing on the relationship between sarcopenia and cardiovascular metabolic diseases, aiming to provide new ideas for clinical prevention and treatment of sarcopenia.

Objective: To explore the longitudinal relationship between bullying victimization and sleep quality among rural boarding junior high school students in Hebei Province, and to investigate the chain mediated role of loneliness and rumination, so as to provide evidence for promoting sleep health in the population. Methods: A baseline survey was conducted in May, 2023 (T1) by convenient sampling method, and two rounds of longitudinal surveys were conducted in November, 2023 (T2) and May, 2024 (T3) among students in two rural boarding junior high schools in Hebei Province, and a sample of 601 students who completed all the surveys was finally obtained. Students completed questionnaires, including the Delaware Bullying Victimization Scale Student (DBVS-S), the University of California at Los Angeles Loneliness Scale (UCLA), the Ruminative Responses Scale, and the Pittsburgh Sleep Quality Index (PSQI). Group differences were examined by using t-test or ANOVA, correlations between variables were analyzed by using Pearson correlation coefficients, and a serial mediation structural equation model was constructed, with mediation effects tested via the Bootstrap method. Results: Female students scored higher on sleep quality than male students (7.47±2.70, 6.47 ±2.46, t =4.74, P <0.01). Pearson correlation analysis indicated that bullying victimization was positively correlated with loneliness, rumination, and sleep quality; loneliness was positively correlated with rumination and sleep quality; and rumination was positively correlated with sleep quality ( r =0.26, 0.33, 0.23; 0.39, 0.38; 0.54, all P <0.01). Mediation analysis showed that T2 loneliness had an independent mediating effect of 0.70 (95% CI =0.36-1.35) between T1 bullying victimization and T3 sleep quality, T2 rumination had an independent mediating effect of 1.34 (95% CI =0.71-2.45), and the serial mediation effect of T2 loneliness and T2 rumination was 0.64 (95% CI =0.37-1.13), accounting for 22.11% of the total effect (all P <0.01). Conclusions Bullying victimization adversely affects sleep quality among rural boarding junior high school students through a longitudinal chain mediating pathway involving loneliness and rumination. Psychological interventions should be strengthened for students who experience bullying to alleviate their loneliness and reduce rumination, thereby improving sleep quality.

Objective To distinguish Cremastra appendiculata(D.Don)Makino,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and its easily confusing products Oreorchis patens and Iphigenia indica Kunth using the ITS sequence in DNA barcodes;To explore the genetic diversity of Cremastra appendiculata germplasm resources.Methods Three different original Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and their easily confusing products Cremastrae Pseudobulbus of Oreorchis patens and Iphigenia indica Kunth were selected as the research objects,and the genomic DNA of the above samples were extracted by the modified CTAB method,and then the ITS sequences were amplified,sequenced and spliced by PCR technology.The Kimura 2-Parameter(K2P)model was used to calculate the genetic distance,and the phylogenetic tree was constructed with the help of neighbour joining method(NJ)for genetic relationship analysis.Results Except for the Iphigenia indica Kunth species that were not found during the BLAST search,the BLAST comparison results of the other samples were higher than 95%.At the same time,the results of phylogenetic tree showed that Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides were clustered into one branch,respectively,and the easily confusing products were also respectively clustered into one branch.Conclusion The ITS sequence in DNA barcodes can be used to accurately distinguish Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and its easily confusing products Oreorchis patens and Iphigenia indica Kunth.

OBJECTIVE To evaluate the effectiveness and safety of warfarin anticoagulation therapy guided by gene test in patients undergoing left ventricular assist device （LVAD） implantation， and to analyze the influencing factors of warfarin anticoagulation efficacy. METHODS Patients who underwent LVAD implantation at the Heart and Vascular Center of Northern Jiangsu People’s Hospital from January 2023 to October 2024 and required warfarin anticoagulant therapy were selected as the study subjects. They were divided into genetic testing group （n＝51） and empirical treatment group （n＝17） based on whether they underwent CYP2C9 and VKORC1 gene test. The gene test group was given warfarin based on the predicted dose calculated by gene test， while the empirical treatment group was given warfarin by clinical doctors based on international normalized ratio （INR） experience， all patients were given warfarin once a day. Follow-up observation was conducted for 6 months to compare the effectiveness [time in therapeutic range（TTR）， the time required to reach INR for the first time， the incidence of embolic events， the incidence of INR＜1.5 events] and safety （the incidence of major and minor bleeding events，the incidence of INR＞3.5 events） of warfarin treatment between two groups of patients. According to whether the patient’s TTR was ≥60%， they were divided into TTR≥60% group （n＝20） and TTR＜60% group （n＝48）. Univariate and multivariate binary Logistic regression analysis were used to determine the factors affecting the anticoagulant effect of warfarin in patients. RESULTS The TTR of patients in the gene test group was significantly higher than that in the empirical treatment group （P＜0.05）. The incidence of INR＜1.5 events in the gene test group was significantly lower than in the empirical treatment group （P＜0.05）. The incidence of minor bleeding events and INR＞3.5 events in the gene test group were lower than in the empirical treatment group， but the difference was not statistically significant （P＞0.05）. The results of multivariate binary Logistic regression analysis showed that gene test was an independent protective factor for warfarin anticoagulant therapy [odds ratio （OR）＝10.842， 95% confidence interval （CI）： 1.211-27.037， P＝0.033]， and the combination of statins was an independent risk factor for warfarin anticoagulant therapy [OR＝0.196， 95%CI： 0.045-0.861， P＝0.031]. CONCLUSIONS Under the guidance of gene test， warfarin anticoagulation therapy for LVAD patients after implantation can improve TTR， shorten the anticoagulation target time， and has good safety； meanwhile， it should be noted that the combination of statins may enhance the anticoagulant effect of warfarin， thereby increasing the risk of bleeding in patients.

Objective:To analyze the risk factors for dysphagia in older adult patients with Parkinson's disease.Methods:The clinical data of 100 older adult patients with Parkinson's disease admitted to Zhejiang Xin'an International Hospital between January 2022 and December 2023 were retrospectively analyzed. The patients were divided into two groups based on the presence of dysphagia: an occurrence group ( n = 40) and a non-occurrence group ( n = 60). General data and clinical characteristics of both groups were compared. Multivariate logistic regression analysis was performed to analyze the risk factors associated with the occurrence of dysphagia in older adult patients with Parkinson's disease. Results:In the cohort of 100 patients, the percentages of those aged 70 years and above, those with a Parkinson's disease H-Y classification greater than 3, those with an Eating Assessment Tool-10 (EAT-10) score of 3 or higher, and the Unified Parkinson's Disease Rating Scale (UPDRS) score in the occurrence group were 75.00% (30/40), 75.00% (30/40), 80.00% (32/40), and (17.23 ± 1.02), respectively. These measurements were higher than those in the non-occurrence group [16.67% (28/60), 43.33% (26/60), 33.33% (20/60), (13.23 ± 0.48), χ2 = 7.91, 9.77, 20.94, t = 22.44, P < 0.05]. The level of uric acid in the occurrence group was significantly lower than that in the non-occurrence group [(345.63 ± 11.35) μmol/L vs. (375.62 ± 10.24) μmol/L, t = 12.41, P < 0.05]. The results of the multivariate logistic regression analysis indicated that age of 70 years and older, H-Y classification greater than 3, Eating Assessment Tool-10 score of 3 or higher, Unified Parkinson's Disease Rating Scale score, and uric acid level were relevant risk factors for dysphagia in older adult patients with Parkinson's disease ( OR = 3.44, 3.82, 3.86, 3.60, 3.71, all P < 0.05). Conclusions:Age of 70 years and older, H-Y classification greater than 3, Eating Assessment Tool-10 score of 3 or higher, Unified Parkinson's Disease Rating Scale score, and uric acid level are relevant risk factors for dysphagia in older adult patients with Parkinson's disease.

Objective:To analyze the risk factors for dysphagia in older adult patients with Parkinson's disease.Methods:The clinical data of 100 older adult patients with Parkinson's disease admitted to Zhejiang Xin'an International Hospital between January 2022 and December 2023 were retrospectively analyzed. The patients were divided into two groups based on the presence of dysphagia: an occurrence group ( n = 40) and a non-occurrence group ( n = 60). General data and clinical characteristics of both groups were compared. Multivariate logistic regression analysis was performed to analyze the risk factors associated with the occurrence of dysphagia in older adult patients with Parkinson's disease. Results:In the cohort of 100 patients, the percentages of those aged 70 years and above, those with a Parkinson's disease H-Y classification greater than 3, those with an Eating Assessment Tool-10 (EAT-10) score of 3 or higher, and the Unified Parkinson's Disease Rating Scale (UPDRS) score in the occurrence group were 75.00% (30/40), 75.00% (30/40), 80.00% (32/40), and (17.23 ± 1.02), respectively. These measurements were higher than those in the non-occurrence group [16.67% (28/60), 43.33% (26/60), 33.33% (20/60), (13.23 ± 0.48), χ2 = 7.91, 9.77, 20.94, t = 22.44, P < 0.05]. The level of uric acid in the occurrence group was significantly lower than that in the non-occurrence group [(345.63 ± 11.35) μmol/L vs. (375.62 ± 10.24) μmol/L, t = 12.41, P < 0.05]. The results of the multivariate logistic regression analysis indicated that age of 70 years and older, H-Y classification greater than 3, Eating Assessment Tool-10 score of 3 or higher, Unified Parkinson's Disease Rating Scale score, and uric acid level were relevant risk factors for dysphagia in older adult patients with Parkinson's disease ( OR = 3.44, 3.82, 3.86, 3.60, 3.71, all P < 0.05). Conclusions:Age of 70 years and older, H-Y classification greater than 3, Eating Assessment Tool-10 score of 3 or higher, Unified Parkinson's Disease Rating Scale score, and uric acid level are relevant risk factors for dysphagia in older adult patients with Parkinson's disease.

Objective To distinguish Cremastra appendiculata(D.Don)Makino,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and its easily confusing products Oreorchis patens and Iphigenia indica Kunth using the ITS sequence in DNA barcodes;To explore the genetic diversity of Cremastra appendiculata germplasm resources.Methods Three different original Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and their easily confusing products Cremastrae Pseudobulbus of Oreorchis patens and Iphigenia indica Kunth were selected as the research objects,and the genomic DNA of the above samples were extracted by the modified CTAB method,and then the ITS sequences were amplified,sequenced and spliced by PCR technology.The Kimura 2-Parameter(K2P)model was used to calculate the genetic distance,and the phylogenetic tree was constructed with the help of neighbour joining method(NJ)for genetic relationship analysis.Results Except for the Iphigenia indica Kunth species that were not found during the BLAST search,the BLAST comparison results of the other samples were higher than 95%.At the same time,the results of phylogenetic tree showed that Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides were clustered into one branch,respectively,and the easily confusing products were also respectively clustered into one branch.Conclusion The ITS sequence in DNA barcodes can be used to accurately distinguish Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and its easily confusing products Oreorchis patens and Iphigenia indica Kunth.

OBJECTIVE To establish the HPLC fingerprint and content determination of five components in Ranunculus sceleratus L.. METHODS The separation was developed on an Agilent ZORBAX SB C18 chromatographic (4.6 mm×250 mm, 5 μm)column by gradient elution with methanol(A)-0.1 % phosphoric acid aqueous solution(B) as mobile phase to establish HPLC fingerprint of Ranunculus sceleratus L.. Combined with similarity evaluation, cluster analysis, principal component analysis, and orthogonal partial least squares-discriminant analysis, the quality of 13 batches of Ranunculus sceleratus L. was evaluated. RESULTS Thirteen batches of Ranunculus sceleratus L. samples were calibrated with 20 common peaks, of which 5 common peaks were identified, and the similarity ranged from 0.874 to 0.984. The results of cluster analysis and principal component analysis were basically the same, indicating that there might be differences in the content of chemical components of Ranunculus sceleratus L. in different regions. Protocatechuic aldehyde, caffeic acid, ferulic acid, hyperoside and isoquercitrin were determined in thirteen batches of Ranunculus sceleratus L., and their contents were 0.016−0.035, 0.010−0.070, 0.010−0.029, 0.016−0.051, 0.028−0.086 mg·g–1, respectively. CONCLUSION The established HPLC fingerprint and content determination method is simple, stable, and reliable, which can be used for qualitative analysis and provide reference to quality evaluation and resource utilization of Ranunculus sceleratus L..

This article reports a rare case of systemic lupus erythematosus (SLE) complicated with rectal ulcer and stenosis, with difficulty in defecation as the main manifestation. Through multiple multidisciplinary consultations and joint decision-making between doctors and patients, combined with surgical and pathological comprehensive diagnosis, monism is considered: SLE affects the rectum. The patient has a good prognosis after undergoing medication, endoscopic local treatment, and surgical treatment.

This article reports a rare case of systemic lupus erythematosus (SLE) complicated with rectal ulcer and stenosis, with difficulty in defecation as the main manifestation. Through multiple multidisciplinary consultations and joint decision-making between doctors and patients, combined with surgical and pathological comprehensive diagnosis, monism is considered: SLE affects the rectum. The patient has a good prognosis after undergoing medication, endoscopic local treatment, and surgical treatment.