Objective To investigate the prevalence, distribution characteristics, and influencing factors of mental health problems among the elderly, and to provide a scientific basis for policy-making.  Methods A convenience sampling method was used to investigate depression, anxiety, and cognitive function among permanent residents aged 65 and older at 59 mental health care sites for the elderly in Hubei Province. Multinomial logistic regression was employed to analyze influencing factors. Results The screening rates for depression, anxiety, and cognitive function at critical/high-risk levels among the elderly in Hubei Province were 9.7%, 5.4%, and 12.2%, respectively. Urban elderly had lower risks of depression and cognitive function at critical/high-risk levels compared to rural elderly (OR for critical depression = 0.640, P < 0.001; OR for high-risk depression = 0.595, P = 0.012; OR for critical cognitive function = 0.448, P < 0.001; OR for high-risk cognitive function = 0.188, P < 0.001). Six key population groups had higher risks of depression, anxiety, and cognitive function at critical/high-risk levels than others (OR for critical depression = 1.463, P < 0.001; OR for high-risk depression = 1.912, P < 0.001; OR for critical anxiety = 1.462, P < 0.001; OR for high-risk anxiety = 2.882, P < 0.001; OR for critical cognitive function = 1.381, P < 0.001; OR for high-risk cognitive function = 2.345, P < 0.001). A higher number of chronic diseases was associated with increased risks of critical and high-risk depression (OR for critical = 1.316, P < 0.001; OR for high-risk = 3.677, P < 0.001) and cognitive impairment (OR for critical depression = 1.316, P < 0.001; OR for high-risk depression = 3.677, P < 0.001; OR for critical anxiety = 1.512, P < 0.001; OR for high-risk anxiety = 1.801, P < 0.001). Conclusion It is recommended to expand mental health care sites in rural areas, improve the layout of mutual-support elderly care facilities, and explore sustainable models for rural elderly care. Efforts should also focus on enhancing social participation among the elderly through community-based activities, and strengthening cognitive screening and emotional regulation interventions, with particular attention to the mental health needs of older, isolated, and chronically ill individuals.

Objective To investigate the relationship between the expression of discoidin,CUB and LCCL domain containing protein 1(DCBLD1)and cytoskeleton associated protein 2(CKAP2)in cervical cancer(CC)tissues and epithelial mesenchymal transition(EMT)and clinical prognosis.Methods 94 CC patients diagnosed and treated in Shiyan Hospital of Traditional Chinese Medicine from February 2017 to February 2019 were selected.The expression of DCBLD1 messenger ribonucleic acid(mRNA),CKAP2 mRNA,N-cadherin(N-cad)mRNA,vimentin(Vim)mRNA and TWIST mRNA in tissues was detected by real-time fluorescence quantitative PCR(qRT-PCR).The expressions of DCBLD1 and CKAP2 were detected by immunohistochemistry(IHC).Pearson correlation analysis was used to analyze the relationship between DCBLD1 mRNA,CKAP2 mRNA and EMT-related indicators.Kaplan-Meier survival curve was drawn to compare the prognosis of CC patients with different DCBLD1 mRNA and CKAP2 mRNA expression.COX regression analysis was used to analyze the prognostic factors of CC patients.Results The expression of DCBLD1 mRNA,CKAP2 mRNA,N-cad mRNA,Vim mRNA and TWIST mRNA in CC cancer tissues was higher than that in adjacent tissues,and the differences were statistically significant(t=32.763～52.824,all P<0.05).The positive rates of DCBLD1 protein(89.36%)and CKAP2 protein(87.23%)in CC cancer tissues were higher than those in adjacent normal tissues(7.45%,6.38%),and the differences were statistically significant(χ2=126.278,123.396,all P<0.001).The expression of DCBLD1 mRNA and CKAP2 mRNA in CC cancer tissues were positively correlated with the expression of N-cad mRNA,Vim mRNA and TWIST mRNA(r=0.655～0.744,all P<0.001).The expression of DCBLD1 mRNA and CKAP2 mRNA in patients with FIGO stage IB2～IIB and lymph node metastasis were higher than those in patients with stage IA～IB1 and without lymph node metastasis(t=25.644～35.674,all P<0.05).The 5-year progression free survival rates of the high expression groups of DCBLD1 mRNA and CKAP2 mRNA were 63.04%and 62.22%,respectively,which were lower than those of the low expression groups of DCBLD1 mRNA and CKAP2 mRNA(91.67%and 91.84%),respectively,and the differences were statistically significant(Log-Rank χ2=7.181,6.527,all P<0.05).FIGO stage IB2～IIB,high DCBLD1 mRNA and high CKAP2 mRNA were risk factors affecting the prognosis of CC patients(Wald χ2=8.277,15.877,10.927,all P<0.05).Conclusion The expression of DCBLD1 and CKAP2 in CC cancer tissues is significantly increased,which is related to EMT related indicators and plays a promoting role in the progression of CC tumors.They are new prognostic markers for CC.

Objective:To investigate the intervention effect of five elements music of traditional Chinese medicine and western classical music on the sleep quality of stroke patients, and to compare the difference between the two, to provide a reference for the clinical care measures to improve the sleep quality of stroke patients.Methods:By adopting a randomized controlled trial, 75 stroke patients who were hospitalized in the rehabilitation hospital of the Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine from July 2022 to July 2023 were selected by convenience sampling method as the study subjects, and the patients were randomly divided the conventional treatment group, the five-element music group, and the classical music group according to the method of randomized numerical table with 25 cases in each group. On the basis of general treatment, the conventional treatment group took sleep health education, the five elements music group were given five-element music intervention based on conventional treatment, and the classical music group were given classical music intervention based on conventional treatment. The changes of Athens Insomnia Scale (AIS), Pittsburgh Sleep Quality Index (PSQI) and polysomnography (PSG) examination parameters in the 3 groups were compared.Results:There were 2 cases of shedding in the conventional treatment group, and 23 cases were finally included, 15 males and 8 females, aged (54.02 ± 7.80) years; there were no cases of shedding in the five-element music group, and 25 cases were finally included, 12 males and 13 females, aged (53.69 ± 6.02) years; and there was 1 case of shedding in the classical music group, and 24 cases were finally included, 10 males and 14 females, aged (52.34 ± 7.08) years. Comparison of AIS, PSQI and PSG scores among the 3 groups of patients before intervention showed no statistically significant differences (all P>0.05). After the intervention, the AIS scores and PSQI scores of the 3 groups were (9.48 ± 1.53) and (12.22 ± 2.94), (6.76 ± 1.36) and (7.64 ± 2.08), (7.46 ± 1.38) and (10.33 ± 2.82), respectively, and the differences were statistically significant ( F=23.21, 18.44, both P<0.05). PSG sleep structure parameters showed that the total sleep time, REM latency time and sleep efficiency of the patients in the five-element music group after the intervention were (399.89 ± 51.76) min, (136.26 ± 25.36) min, (78.87 ± 8.21)%, higher than (368.45 ± 47.88) min, (124.46 ± 26.25) min, (73.36 ± 7.86)% in the classical music group and (345.48 ± 38.69) min, (111.37 ± 23.23) min, (69.44 ± 7.88)% in the conventional treatment group, and the differences between three groups were statistically significant ( F=8.27, 5.93, and 8.49, all P<0.05); the sleep latency time, awakening time, and number of awakenings in the five-element music group were (28.86 ± 17.68) min, (54.37 ± 25.15) min, and (2.72 ± 1.19) times, respectively, which were lower than those in the classical music group (35.67 ± 16.99) min, (64.28 ± 29.34) min, and (3.67 ± 1.12) times and (42.38 ± 18.96) min, (78.38 ± 37.26) min, (4.87 ± 1.46) times in the conventional treatment group, and the differences between three groups were statistically significant ( F=3.51, 3.66, and 17.56, all P<0.05). The results of the PSG sleep progression showed that the duration of the N1 stage of sleep in the five-element music group after the intervention was (95.71 ± 15.23) min, which was higher than (83.20 ± 18.34) min in the classical music group and (80.93 ± 16.47) min in the conventional treatment group, and the difference between three groups was statistically significant ( F=5.53, P<0.01); the N3 stage sleep time and the sleep percentage of the five-element music group after the intervention were respectively (84.23 ± 20.98) min and (23.98 ± 5.89)%, which were higher than (65.33 ± 18.82) min and (18.34 ± 3.78)% in the classical music group and (45.87 ± 18.65) min and (15.03 ± 5.56)% in the conventional treatment group, and the differences between three groups were statistically significant ( F=23.08, 18.50, both P<0.05). Conclusions:Both five elements music and classical music can improve the sleep quality of stroke patients, and the effect of five elements music to improve sleep is more significant.

Objective:To explore the risk factors and prediction model of lateral lymph node metastasis(LLNM)in Hashimo-to's thyroiditis(HT)combined with papillary thyroid carcinoma(PTC).By investigating its risk factors in a multi-dimensional and sys-tematic manner,a precise prediction model with clinical application value will be constructed to provide a basis for clinical diagnosis and treatment,prognosis assessment,and surgical practice decision evaluation.Methods:A retrospective analysis was conducted on the clinical data of 201 patients with HT combined with PTC who were admitted to the Thyroid Surgery Department of the First Affiliated Hospital of Jinzhou Medical University from April 2022 to June 2024.According to whether the patients had LLNM,they were divided into a metastasis group(n=82)and a non-metastasis group(n=119).The relevant clinical indicators of the two groups of patients were compared.Univariate and multivariate analyses were used to identify the independent risk factors affecting the occurrence of LLNM in patients.A logistic regression was employed to establish a prediction model and evaluate its predictive value.Results:The results of univariate analysis showed that there were statistically significant differences between the two groups in terms of TSH value,nodule lo-cation,nodule echo,shape,boundary,aspect ratio,tumor diameter,multifocality,microcalcification,rich blood supply of nodules,ELN,CLNM,capsule invasion,and ETE(P<0.05).The results of multivariate logistic regression analysis indicated that tumor diam-eter,CLNM,rich blood supply of nodules,multifocality,and TSH were all independent risk factors for LLNM(P<0.05).The receiv-er operating characteristic(ROC)curve analysis demonstrated that the areas under the curve(AUC)of tumor diameter,multifocality,CLNM,rich blood supply of nodules,and TSH were 0.613(95%CI:0.533～0.693),0.656(95%CI:0.578～0.733),0.694(95%CI:0.619～0.768),0.622(95%CI:0.543～0.701)and 0.588(95%CI:0.510～0.667),respectively.The sensitivities were 72.20%,69.60%,65.80%,60.80%and 41.80%,respectively and the specificities were 45.90%,62.30%,73.80%,60.70%and 83.60%,respectively.Conclusion:Tumor diameter,multifocality,CLNM,rich blood supply of nodules and TSH are significantly associated with the occur-rence of LLNM in patients with HT combined with PTC.Among them,tumor diameter and multifocality had relatively high sensitivities in predicting LLNM and has a certain predictive value for the occurrence of LLNM in patients with HT combined with PTC.

Tumor-related insomnia is one of the common complications of tumor patients,which is secondary to tumor disease and related to tumor disease itself or tumor treatment.Combined with the unique pathogenesis of"heat-toxicity internal stagnation"of tumor-related insomnia,the important treatment methods are to clear away heat,attack toxicity,regulate qi and supplement healthy qi.This article explained the research status,etiology and pathogenesis,treatment principles of the disease,in order to provide new ideas and methods for the differentiation and treatment of tumor-related insomnia in TCM.

Objective To explore the efficacy of endoscopic thyroidectomy via subclavian single incision in treatment of pa-tients with thyroid cancer.Methods In this study,132 thyroid cancer patients who underwent thyroid surgery at the First Affil-iated Hospital of Jinzhou Medical University from July 2022 to June 2024 were selected.Based on the surgical approach,the pa-tients weredivided into Group A(traditionalanterior neck approach open surgery,68 cases)and Group B(subclavicular single-in-cision laparoscopic surgery,64 cases).The surgical-related indicators,pain levels,thyroid function,incision cosmetic satisfac-tion,and postoperativecomplications werecompared between the two groups.Results The surgery time in Group B was longer than that in Group A,but the intraoperative blood loss in Group B was lower than that in Group A,and the postoperative drain-age volume in Group B was higher than in Group A(all P＜0.05).There was no significant difference in the drainage tube re-moval time and hospitalization duration between the two groups(all P＞0.05).The VAS scores at 3 days,7 days,and 1 month after surgery were all lower in Group B than in Group A(all P＜0.05).Thelevels of TSH,FT3,and FT4 at3 days post-surgery were higher in Group B than in Group A(all P＜0.05).At 3 months post-surgery,the cosmetic satisfaction with the incision in Group B was higher than in Group A(χ2=10.534,P=0.001).There was no significant difference in the overall incidence of postoperative complications between the two groups(χ2=0.706,P=0.401).Conclusion Endoscopic thyroidectomy via subcla-vian single incision is safe and reliable,with less blood loss,less postoperative pain,less influence on thyroid function and high cosmetic satisfaction.

Objective:To explore the associations of temperature, relative humidity, and their interaction effect with fall risk.Methods:Data on fall cases were collected using the national injury surveillance system from May to September, in 2006-2022 in Hubei Province. Combined with the meteorological and air pollution data, we conducted a time-stratified case-crossover design and used conditional logistic regression models and distributed lag nonlinear models to examine the exposure-response relationships between temperature, humidity, and fall risk. We further divided the low and the high temperature groups and the low and the high relative humidity groups and analyzed the excess risk ( ER) of falls attributed to dry-hot or wet-hot events. Finally, we calculated the additive interactions of temperature and humidity on fall risk. Results:A total of 55 401 fall cases were included. With the increase in temperature and relative humidity decrease, the exposure-response curves of fall showed nonlinear upward trends among all populations. Gender and age differences were found in temperature-fall and relative humidity-fall risk relationships. Compared with wet-non-hot (normal temperature and high relative humidity) events, the ER of fall in dry-hot (high temperature and low relative humidity) events was 14.80% (95% CI: 9.69%- 20.15%), and the ER of wet-hot (high temperature and high relative humidity) events was 9.59% (95% CI: 2.52%-17.13%). However, there was no statistically significant difference between dry-hot and wet-hot events in the fall, and no statistically significant difference between different genders, ages, occupations, and fall occurred place (all P>0.05). No significant synergistic additive interaction was found between temperature and relative humidity on fall risk (relative excess risk due to interaction=-0.08, 95% CI: -0.19-0.02). Conclusions:Higher temperatures and lower relative humidity were associated with increased fall risk. Both dry-hot and wet-hot events had a higher risk of fall, while high temperature and low humidity have no synergistic effect on fall risk.

Objective To explore the efficacy of endoscopic thyroidectomy via subclavian single incision in treatment of pa-tients with thyroid cancer.Methods In this study,132 thyroid cancer patients who underwent thyroid surgery at the First Affil-iated Hospital of Jinzhou Medical University from July 2022 to June 2024 were selected.Based on the surgical approach,the pa-tients weredivided into Group A(traditionalanterior neck approach open surgery,68 cases)and Group B(subclavicular single-in-cision laparoscopic surgery,64 cases).The surgical-related indicators,pain levels,thyroid function,incision cosmetic satisfac-tion,and postoperativecomplications werecompared between the two groups.Results The surgery time in Group B was longer than that in Group A,but the intraoperative blood loss in Group B was lower than that in Group A,and the postoperative drain-age volume in Group B was higher than in Group A(all P＜0.05).There was no significant difference in the drainage tube re-moval time and hospitalization duration between the two groups(all P＞0.05).The VAS scores at 3 days,7 days,and 1 month after surgery were all lower in Group B than in Group A(all P＜0.05).Thelevels of TSH,FT3,and FT4 at3 days post-surgery were higher in Group B than in Group A(all P＜0.05).At 3 months post-surgery,the cosmetic satisfaction with the incision in Group B was higher than in Group A(χ2=10.534,P=0.001).There was no significant difference in the overall incidence of postoperative complications between the two groups(χ2=0.706,P=0.401).Conclusion Endoscopic thyroidectomy via subcla-vian single incision is safe and reliable,with less blood loss,less postoperative pain,less influence on thyroid function and high cosmetic satisfaction.

Objective : To investigate the effects of cinnamaldehyde(CA) on the growth, metastasis and apoptosis of human endometriosis(EMs) cells and to explore whether the mechanism is related to ribosomal protein S7(RPS7) expression. Methods : Endometriosis cells were divided into control group, CA group, sh-NC group, CA+sh-RPS7 group. Effects of CA on cell growth in human endometriotic cells were determined using Cell Counting Kit-8(CCK-8) and colony formation assay. Effects of CA on cell metastasis were performed by motility assay and Transwell assay. Effects of CA on cell apoptosis were evaluated by Hoechst 33258 staining and flow cytometry. Meanwhile, the levels of PCNA, E-cadherin, Vimentin, Bax and Bcl-2 were evaluated using Western blot in human endometriotic cells with treatment CA. The expression of RPS7 was detected by qRT-PCR and Western blot assay. The RPS7 overexpression of human endometriotic cells was established by cell transfection. CA-mediated effects on cell proliferation and apoptosis were determined by CCK-8 assay and flow cytometry in human endometriotic cells with RPS7 overexpression. Results : CA repressed cell growth as well as down-regulated PCNA. The half inhibitory concentration(IC50) value was 53.60 μmol/L after 24 h treatment, and colony formation rate was 25.32%. Additionally, CA inhibited metastasis which was associated with downregulated Vimentin and upregulated E-cadherin. The relative migration rates were 35% and 29% as well as invasion rate was 40%. Further, CA induced apoptosis by cell cycle G2/M phase arrest and cell apoptosis rate was 25.1%, which related to the up-regulation of of Bax and the down-regulation of Bcl-2. CA inhibited the expression of RPS7 and overexpression of RPS7 promoted cell proliferation and suppressed apoptosis in CA-mediated cells. Conclusion CA inhibits cell growth, metastasis, and induces cell apoptosis by downregulating the expression of RPS7.

Abstract CCAAT/enhancer binding protein β(C/EBPβ) is a transcription factor that plays a vital role in diverse biological processes,including cell proliferation,cell apoptosis,inflammation,and immune regulation.It is expressed in female reproductive organs such as the ovary and uterus,and it serves as a critical regulator of both physiological functions and pathological conditions.This review comprehensively summarizes the significant contributions of C/EBPβ to physiological processes such as follicular development and decidualization,as well as its involvements and mechanisms in reproductive disorders including polycystic ovary syndrome,endometriosis,and ovarian tumors,aiming to provide a potential research target in the field of reproduction.