Objective: To systematically evaluate the lapse rate of voluntary blood donation in China, and to provide theoretical reference for blood centers to develop donor retention strategies. Methods: Cross-sectional studies on the lapse rate of voluntary blood donation and its influencing factors, published between January 2015 and May 2024, were retrieved from the CNKI, Wanfang Data, VIP, PubMed, Web of Science and The Cochrane Library databases. Two reviewers independently screened the literature and extracted data, and Review Manager 5.4 software was used for meta-analysis. Results: A total of 14 literature involving 428 343 lapsed blood donors were included. Meta-analysis showed that the overall lapse rate of blood donation in China was 47.0% [95% CI(33.0, 61.0)]; and age among 18 to 25[OR=1.40, 95% CI(1.02, 1.92)], no previous donation history [OR=4.54, 95% CI(2.13, 9.67)], relocation from the city of last donation [OR=2.89, 95% CI(1.13, 7.37)], low satisfaction [OR=1.80, 95% CI(1.42, 2.28)], adverse reactions [OR=1.92, 95% CI(1.07, 3.45)], and negative public opinion [OR=2.72, 95% CI(1.23, 6.02)] were the influential factors for blood donors. The sub group analysis results showed that the lapse rate of blood donation in China during different research period was 44.0%[95% CI (26.7, 62.4)] from 2017 to 2019 and 50.0%[95% CI(37.2, 63.1)] from 2020 to 2023; and the lapse rate of blood donors was 42.0%[95% CI(31.8, 52.8)] among platelet donors and 64.0%[95% CI(52.2, 83.2)] among whole blood donors. Sensitivity analysis suggests that the research results are stable, and the funnel diagram combined with Egger test and Begg' test shows that there is no publication bias. Conclusion: The lapse rate of blood donation remains relatively high in China. Blood centers should take measures such as strengthening guidance for first-time blood donors, promoting refined services, implementing predictive interventions, and improving to monitor the social media to enhance blood donor's willingness for donating repeatedly and reduce the lapse rate of blood donors.

ObjectiveTo establish a quality evaluation method for Zhuye Shigao granules（Zhuye Shigaotang） based on fingerprint and determination of index components， and to investigate the therapeutic effect of Zhuye Shigao granules on mice with cold-dampness pestilence attacking lung syndrome. MethodsThe fingerprint of Zhuye Shigao granules was established by high performance liquid chromatography（HPLC）， and the methods for determination of total calcium， orientin， isoorientin， ginsenosides Rg₁， Re and Rb₁ and other 2 index components were established. Fifty ICR mice were randomly divided into the blank group， model group， Zhuye Shigao granules low， medium and high dose groups（9.3， 18.6， 37.2 g·kg^-1·d^-1）， with 10 mice in each group. In addition to the blank group， the model mice with cold-dampness pestilence attacking lung syndrome was prepared by nasal drip of lipopolysaccharide combined with cold-dampness environment. Each administration group was given the corresponding liquid by gavage according to the dose， while the blank group and model group were given the same volume of normal saline by gavage. Then， the body temperature and organ index of mice in each group were measured， hematoxylin-eosin（HE） staining was used to investigate the lung tissue injury of mice in each group， and enzyme-linked immunosorbent assay（ELISA） was used to detect the changes of tumor necrosis factor-α（TNF-α）， interleukin（IL）-lβ， IL-6， IL-10 levels in serum and lung tissue， as well as immunoglobulin（Ig）A and IgM levels in serum. ResultsThe fingerprint similarity of 10 batches of Zhuye Shigao granules was>0.950， and 20 common peaks were calibrated. Seven of them were identified， including peak 11（isoorientin）， peak 12（orientin）， peak 14（apioside liquiritin）， peak 15（liquiritin）， peak 17（apioside isoliquiritin）， peak 19（isoliquiritin） and peak 20（liquiritigenin）. The results of quantitative analysis showed that the content range of each index component in 10 batches of Zhuye Shigao granules was as follows：Total calcium of 9.978-11.294 mg·g^-1， isoorientin of 0.033-0.041 mg·g^-1， orientin of 0.046-0.055 mg·g^-1， ginsenoside Rg₁+ginsenoside Re of 0.748-0.762 mg·g^-1， ginsenoside Rb₁ of 0.151-0.197 mg·g^-1， liquiritin of 1.106-1.366 mg·g^-1， glycyrrhizic acid of 0.904-1.182 mg·g^-1_. Compared with the blank group， the body temperature of mice in the model group was significantly increased， the organ indexes of liver， lung and spleen were significantly decreased， the organ index of thymus was significantly increased， HE staining of lung tissue showed infiltration of inflammatory cells， a small amount of serous exudation was observed in the alveoli， and lung tissue was damaged. After the intervention of Zhuye Shigao granules， the pathological changes were improved compared with the model group. The expression levels of IL-1β， IL-6 and TNF-α were significantly increased， the expression level of IL-10 was significantly decreased in serum and lung tissue. The levels of IgA and IgM in serum were significantly decreased（P<0.01）. Compared with the model group， the body temperature， the organ indexes and immune factor levels in serum and lung tissue of mice in the Zhuye Shigao granules medium and high dose groups were significantly reduced（P<0.05， P<0.01）. ConclusionIn this study， the quality evaluation of Zhuye Shigao granules was carried out based on fingerprint combined with determination of index components， and the fingerprint of four herbs（Lophatheri Herba， Ophiopogonis Radix， Pinelliae Rhizoma and Glycyrrhizae Radix et Rhizoma） in this formula and the determination of 8 index components were established. The therapeutic effect of Zhuye Shigao granules on mice with cold-dampness pestilence attacking lung syndrome may be related to inhibiting inflammatory response and mediating immune regulation.

Objective:To investigate the status quo of the salary status of Guangxi's village doctors under the background of the implementation of"Employed by Townships and Work in the Villages"Policy.Methods:A total of 142 village doctors'salary data were obtained and sorted out through field investigation.Village doctors'salary level was analyzed with Chi-square test,rank test and correlation analysis.Results:More than 70%of village doctors'annual salary in the surveyed counties exceeded 50 000 yuan.Performance allowance is the main component of village doctors'annual salary.There are differences between the implementation number of"Employed by Townships and Work in the Villages"Policy and village doctors'annual salary level in different urban areas(P＜0.05).The village doctors'service population was positively correlated with the allowance of basic public health service,the general medical service population and the annual salary level(P＜0.05).Conclusion:Under the"Employed by Townships and Work in the Villages"Policy,the salary level of Guangxi's village doctors has been improved,and the salary component could be further optimized.It is suggest that the"Employed by Townships and Work in the Villages"Policy should be promoted in accordance with the economic development conditions of different urban areas,the income standards that match village doctors'academic,professional ranks,professional qualifications and working years,and the performance evaluation standards linked to village doctors'subsidy standards should be improved.

Objective:To investigate the status quo of the salary status of Guangxi's village doctors under the background of the implementation of"Employed by Townships and Work in the Villages"Policy.Methods:A total of 142 village doctors'salary data were obtained and sorted out through field investigation.Village doctors'salary level was analyzed with Chi-square test,rank test and correlation analysis.Results:More than 70%of village doctors'annual salary in the surveyed counties exceeded 50 000 yuan.Performance allowance is the main component of village doctors'annual salary.There are differences between the implementation number of"Employed by Townships and Work in the Villages"Policy and village doctors'annual salary level in different urban areas(P＜0.05).The village doctors'service population was positively correlated with the allowance of basic public health service,the general medical service population and the annual salary level(P＜0.05).Conclusion:Under the"Employed by Townships and Work in the Villages"Policy,the salary level of Guangxi's village doctors has been improved,and the salary component could be further optimized.It is suggest that the"Employed by Townships and Work in the Villages"Policy should be promoted in accordance with the economic development conditions of different urban areas,the income standards that match village doctors'academic,professional ranks,professional qualifications and working years,and the performance evaluation standards linked to village doctors'subsidy standards should be improved.

Objective To create a mouse model of colorectal polyps with Apc-Kras-Cre gene mutations using the tamoxifen induction method.Methods Mice with Apc-Kras-Cre mutations were divided into four groups and injected intraperitoneally with different concentrations and dosages of tamoxifen for different durations,with group 1 injected with low dosage tamoxifen(5 mg/kg)for 1 day,group 2 injected with low dosage tamoxifen(5 mg/kg)for 3 days,group 3 injected with high dosage tamoxifen(50 mg/kg)for 1 day,group 4 injected with high dosage tamoxifen(50 mg/kg)for 3 days.C57BL/6J mice were used as a healthy control group and survival and changes in body weight were observed.All mice were euthanized 4 weeks post-tamoxifen induction and the colon length and number and size of intestinal polyps were observed.Histological changes in the intestinal tissue and polyps were detected by hematoxylin and eosin staining.Results The survival rate of male mice was higher(P＜0.001)and the morbidity rate of male mice was lower compared with female mice(P＜0.05).The survival rate differed significantly among the four groups(P＜0.01).All groups showed significant changes in body weight compared with the healthy control group(P＜0.001).There were also significant differences in weight changes between tamoxifen-induced groups 1 and 2,between groups 2 and 3,and between groups 1 and 4(P＜0.001,P＜0.01,P＜0.05,respectively).There were no significant differences in colon length between any treated group and the healthy control group(P＞0.05),but colon length did differ between tamoxifen-induced groups 1 and 3(P＜0.05).Polyp size varied in each group of tamoxifen-treated mice,with most polyps occuring at the distal end of the colon,while mice in groups 3 and 4 had more and larger polyps.Histopathological examination showed intestinal polyps with uneven and misaligned glandular and epithelial arrangements,a loosely-packed intestinal mucosal barrier,and irregularly-distributed crypts in tamoxifen-induced mice compared with the healthy control group,while mice in tamoxifen-induced groups 3 and 4 showed signs of inflammation and mice in group 4 showed necrosis of cells in some regions.Conclusions Tamoxifen-induced Apc-Kras-Cre model mice were successfully established,with the group 3 induction method being the most suitable.

Objective To analyze the current status and influencing factors of occupational burnout among medical staff in Dongguan City. Methods A total of 1 007 medical staff from eight hospitals in Dongguan City were selected as the study subjects using the stratified sampling method. The Maslach Burnout Inventory-Human Service Survey and Moral Injury Symptom Scale (Healthcare Professionals Edition) were used to assess occupational burnout and moral injury among these study subjects. Binary logistic regression analysis was conducted to identify the influencing factors of occupational burnout. Results The detection rate of occupational burnout among the medical staff was 46.2%, and the detection rate of moral injury was 48.1%. The result of binary logistic regression analysis showed that after controlling for confounding factors such as age, marriage status, educational level, religious belief, professional title, and hospital characteristics, male medical staff had a higher risk of occupational burnout than female staff (P<0.01). Medical staff with ≤10 years of work experience had a higher risk of occupational burnout than those with >10 years of work experience(P<0.01). Additionally, medical staff with moral injury had a higher risk of occupational burnout than those without moral injury (P<0.01). Conclusion Occupational burnout is relatively common among medical staff in Dongguan City and is influenced by factors such as gender, working years, moral injury, and others.

A 58-year-old female patient without disease history of diabetes mellitus was enrolled in the phase Ⅲ clinical trial of alpelisib combined with fulvestrant due to ineffective treatment of stage Ⅳ breast cancer. The treatment regimen was alpelisib tablets 300 mg orally once daily and fulvestrant injection 500 mg once a month intramuscularly. One month later, the patient experienced symptoms such as poor appetite, nausea, vomiting, drowsiness, and blurred consciousness. Laboratory tests showed arterial blood pH 7.1, random blood glucose >34.7 mmol/L, serum creatinine 210 μmmol/L, blood potassium 3.00 mmol/L, blood sodium 129 mmol/L, and urine ketone body (++). Diabetic ketoacidosis was diagnosed, and treatments such as intravenous insulin supplementation, rehydration, and correction of acidosis and electrolyte disorders were given. After 5 days, the patient′s symptoms were improved, but the blood glucose was poor controlled. Diabetic ketoacidosis was considered to be related to alpelisib. The dose of alpelisib was reduced by half, oral hypoglycemic drugs were added, and the insulin regimen was adjusted. Later, the blood glucose was relatively well controlled, and urinary ketone bodies turned negative.

A 58-year-old female patient without disease history of diabetes mellitus was enrolled in the phase Ⅲ clinical trial of alpelisib combined with fulvestrant due to ineffective treatment of stage Ⅳ breast cancer. The treatment regimen was alpelisib tablets 300 mg orally once daily and fulvestrant injection 500 mg once a month intramuscularly. One month later, the patient experienced symptoms such as poor appetite, nausea, vomiting, drowsiness, and blurred consciousness. Laboratory tests showed arterial blood pH 7.1, random blood glucose >34.7 mmol/L, serum creatinine 210 μmmol/L, blood potassium 3.00 mmol/L, blood sodium 129 mmol/L, and urine ketone body (++). Diabetic ketoacidosis was diagnosed, and treatments such as intravenous insulin supplementation, rehydration, and correction of acidosis and electrolyte disorders were given. After 5 days, the patient′s symptoms were improved, but the blood glucose was poor controlled. Diabetic ketoacidosis was considered to be related to alpelisib. The dose of alpelisib was reduced by half, oral hypoglycemic drugs were added, and the insulin regimen was adjusted. Later, the blood glucose was relatively well controlled, and urinary ketone bodies turned negative.

Objective:To construct a nursing quality evaluation index system for midline catheters, so as to provide a reference for the objective and scientific evaluation of the nursing quality of midline catheters.Methods:Taking Donabedian's structure-process-outcome as the theoretical framework, the nursing quality evaluation index system for midline catheters was preliminarily formulated through literature review and semi-structured interview. From July to September 2021, two rounds of expert consultation were conducted through Delphi method and analytic hierarchy process to establish the quality evaluation index system and the weight of each index.Results:The constructed nursing quality evaluation index system for midline catheters included three first-level indicators, 14 second-level indicators, and 73 third-level indicators. Among two rounds of consultation, the positive coefficients of experts were all 100%, the authority coefficients were 0.819 and 0.831, and the coordination coefficients were 0.218 and 0.220, respectively.Conclusions:The constructed nursing quality evaluation index system for midline catheters was extremely scientific, practical and feasible, which can provide a reference for clinical nurses to continuously improve the nursing quality of midline catheters.

With the emergence of DNA nanotechnology in the 1980s, self-assembled DNA nanostructures have attracted considerable attention worldwide due to their inherent biocompatibility, unsurpassed programmability, and versatile functions. Especially promising nanostructures are tetrahedral framework nucleic acids (tFNAs), first proposed by Turberfield with the use of a one-step annealing approach. Benefiting from their various merits, such as simple synthesis, high reproducibility, structural stability, cellular internalization, tissue permeability, and editable functionality, tFNAs have been widely applied in the biomedical field as three-dimensional DNA nanomaterials. Surprisingly, tFNAs exhibit positive effects on cellular biological behaviors and tissue regeneration, which may be used to treat inflammatory and degenerative diseases. According to their intended application and carrying capacity, tFNAs could carry functional nucleic acids or therapeutic molecules through extended sequences, sticky-end hybridization, intercalation, and encapsulation based on the Watson and Crick principle. Additionally, dynamic tFNAs also have potential applications in controlled and targeted therapies. This review summarized the latest progress in pure/modified/dynamic tFNAs and demonstrated their regenerative medicine applications. These applications include promoting the regeneration of the bone, cartilage, nerve, skin, vasculature, or muscle and treating diseases such as bone defects, neurological disorders, joint-related inflammatory diseases, periodontitis, and immune diseases.
Nucleic Acids/chemistry* ; Regenerative Medicine ; Consensus ; Reproducibility of Results ; DNA/chemistry*