Objective: To understand the association of maternal emotional symptoms and parent-child relationship with emotional and behavioral problems (EBPs) in primary school students, so as to provide a reference for the prevention of EBPs in primary school students. Methods: In May 2023, a convenient cluster sampling method was used to select 1 324 students from two primary schools in Hefei City, Anhui Province as the study subjects. The Generalized Anxiety Disorder-7 (GAD-7), Patient Health Questionnaire (PHQ-9), Family Adaptation and Cohesion Evaluation ScalesⅡ (FACES Ⅱ), and Strengths and Difficulties Questionnaire (SDQ) of parental version were used to investigate maternal emotional symptoms, parent-child relationship, and EBPs of primary school students, respectively. Multivariate Logistic regression analysis model was used to analyze the association of different maternal emotional symptoms and parent-child relationship with EBPs of primary school students. Results: The abnormal detection rates of emotional symptoms, conduct problems, hyperactivity disorder, peer problems, prosocial behavior and total difficulties in primary school students were 13.6%, 19.4%, 20.2%, 36.5%, 20.9% and 51.9%, respectively. Multivariate Logistic regression analysis showed that the detection rates of EBPs in primary school students whose mothers had anxiety and depression symptoms with low level of parent-child relationship increased ( OR=1.52-6.97, P <0.01). The combined effect of mothers who had anxiety and depression symptoms with low level of parent-child relationship increased the risk of EBPs in all dimensions of primary school students ( OR=4.07-10.14, P <0.01). After gender stratification, the combined effect of mothers who had anxiety and depression symptoms with low level of parent-child relationship increased the risk of EBPs in all dimensions of boys and girls ( OR=3.71- 10.95 , P <0.01). Conclusions Maternal emotional symptoms and parent-child relationship are associated with EBPs in primary school students. Reducing maternal anxiety and depression symptoms, and improving parent-child relationship are beneficial to reduce the incidence of EBPs problems in primary school students.

A 66-year-old male patient with type 2 diabetes mellitus was treated with oral dapagliflozin (10 mg once daily) and pioglitazone hydrochloride and metformin hydrochloride (515 mg twice daily) regularly for more than 5 months and no adverse reactions occurred. Pioglitazone hydrochloride and metformin hydrochloride was changed to acarbose capsules (50 mg thrice daily orally) by himself. Three days later, the patient developed generalized skin itching with fever (at high temperature up to 41.0 ℃), followed by pustules in the size of soybean to broad beans scattering on the whole body. Then acarbose capsules were stopped immediately. After consultation with dermatologists, acute generalized exathematous pustulosis was diagnosed. The patient was treated with dexamethasone for anti-allergy, insulin for management blood sugar, and cefoxitin for anti-infection. On the 4th day after discontinuation of acarbose capsules, the patient′s rash was improved and scab formed at the ulceration site; on the 9th day, the rash subsided.

A 66-year-old male patient with type 2 diabetes mellitus was treated with oral dapagliflozin (10 mg once daily) and pioglitazone hydrochloride and metformin hydrochloride (515 mg twice daily) regularly for more than 5 months and no adverse reactions occurred. Pioglitazone hydrochloride and metformin hydrochloride was changed to acarbose capsules (50 mg thrice daily orally) by himself. Three days later, the patient developed generalized skin itching with fever (at high temperature up to 41.0 ℃), followed by pustules in the size of soybean to broad beans scattering on the whole body. Then acarbose capsules were stopped immediately. After consultation with dermatologists, acute generalized exathematous pustulosis was diagnosed. The patient was treated with dexamethasone for anti-allergy, insulin for management blood sugar, and cefoxitin for anti-infection. On the 4th day after discontinuation of acarbose capsules, the patient′s rash was improved and scab formed at the ulceration site; on the 9th day, the rash subsided.

Objective To analyze the research achievements and their outcome influence factors of the National Natural Science Foundation of China (NSFC) funding projects in Peking University Cancer Hospital during the "Twelfth Five-Year Plan".Methods Based on project data and outcomes of 68 projects supported by NSFC from 2011 to 2015,this paper studies the number of SCI papers,Chinese Journal papers and conference papers published,the citations per paper and talent cultivation of different subcategories of projects,as well as different professional titles,academic degrees,genders,ages,occupations of the principle investigator,and component of project team members.Results 90％ of the 68 projects published SCI papers,basically completed the intended research objectives set in the application.41.7％ of the SCI papers were published in the 1-5 year after the project closure.Research team collaborated between clinical and scientific research staff has higher output.Conclusions Scientific research management department should strengthen the process management and the tracking of project achievements,and should promote the combination of basic and clinical research to achieve mutually complementary.

This study aimed at efficiently screening out the toxic herbal medicines in Zhuang Gu Guan Jie pill (ZGGJ) based on the zebrafish model.Zebrafish embryos at 1 day post fertilization (dpf) were administered by ZGGJ and the water decoction (WD) and ethanol extract (EE) of 12 herbal drugs in it with various concentrations.The number of embryo deaths after the administration was recorded at 2-6 dpf and 2-8 dpf,respectively.Embryonic micro-morphology of zebrafish (3 dpf) was observed,LC50 value at 6 dpf was calculated by SPSS16.0.As a result,both the WD and EE of ZGGJ,fructus psoraleae,radix dipsaci,olibanum,myrrh,radix angelicae pubescentis and aucklandiae radix,and the EE of Caulis Spatholobo and Rhizoma Cibotii presented high organ toxicity to the juvenile zebrafish,featuring yolk sac swelling,deforming,blackening,indicating hepatotoxicity in the zebrafish.Their LC50 value at 6 dpf was from 33.2 μg·mL-1 to 392.7 μg·mL-1 (LD50 value was converted to crude drug.The same bellow.).The WD and EE of Epimedii Folium at 1,500 μg· mL-1 led to yolk sac swelling and blackening.The LC50 at 6 dpf varied at 1,100-1,350 μg·mL-1,showing a certain toxicity of Epimedii Folium.The WD of Caulis Spatholobo and Cibotii Rhizoma and both WD and EE of Rhizoma drynariae,taxilli herba and prepared rehmanniae radix didn' t change the toxicity to the organs in zebrafish.LC50 value were over 1,500 μg·mL-1,presenting favorable safety.In conclusion,it was demonstrated that the zebrafish model efficientlyidentified the toxicity of different herbal medicines from ZGGJ and efficiently screened out the potential toxicants in vivo with the advantages of real-time,dynamic and large scale for traditional Chinese medical compounds.

OBJECTIVE To investigate the bone development activity and differences in safety of ethanol extract (EE) and water decoction (WD) of Psoralea corylifolia L.efficiently.METHODS Zebrafish larvae were co-exposed to prednisolone 25 μmol· L-1 and different concentrations of EE and WD (0.1,1.0,10 and 100 mg crude drug· L-1),and etidronate disodium (ED) 30 mg·L-1.All these groups were incubated at 28.5℃ until 9 dpf.The medium solution was changed every other day.Zebrafish skeleton at 9 dpf was stained with alizarin red and inspected under an optical microscope,in addition,the death toll and organ toxicity of zebrafish were also observed.The mRNA expression of osteoprotegrin (OPG) and receptor activator of NF-κB ligand (RANKL) in 9 dpf zebrafish were determined with fluorescence quantitative PCR.Zebrafish embryos (1 dpf) were exposed to various concentrations of EE (10,20,30,35,40,50 and 60 mg crude drug· L-1),WD (10,50,100,125,150,175,200 and 500 mg crude drug· L-1),psoralen (12.5,25.0,50.0,100.0,200.0 and 400.0 μmol·L-1) and bakuchiol (1,5,10,25 and 50 μmol· L-1).Embryonic morphology of zebrafish (3 dpf) was inspected with an optical microscope and the death toll of embryos or larvale was counted from 2 dpf to 9 dpf and LC50was calculated.Components of EE and WD ware analyzed by HPLC method.RESULTS Both EE (0.1 mg crude drug· L-1) and WD (1.0 mg crude drug· L-1) groups could increase the staining area and optical density values of zebrafish skeleton compared with prednisolone group (P＜0.01),indicating the increase in bone mineralization;the OPG mRNA expression in both EE and WD (1.0 mg crude drug· L-1) groups increased,while the RANKL mRNA expression decreased (P＜0.01) and the ratio of OPG/RANKL improved obviously (P＜0.01).Embryos exposed to EE,WD,psoralen and bakuchiol showed swelling of the heart and yalk sac,and decrease in GOT.The LC50 of WD and psoralen was 5～8 and 5～21 times that EE and bakuchiol,respectively.The composition and relative content of EE and WD also varied considerably.CONCLUSION Bone development activity and toxicity of EE are both stronger than those of WD.The lipid soluble characteristic components of Psoralea corylifolia L.,may be critical components of toxicity.

Objective To investigate the expression of glucagon-like peptide-1 receptor (GLP-1R) of skeletal muscle cells and the changes of skeletal muscle microstructure of diabetic rats, and to explore the relationship between the expressions of GLP-1R and insulin resistance in skeletal muscle. Methods Male Wistar rats were divided into two groups, normal glucose tolerance ( NT, n =25 ) , diabetes group ( DM group, n=30), the methods of creating diabetes model were used high-sugar high-fat diet and low-dose in-traperitoneal injection of streptozotocin. Immunohistochemical methods was used to detect the expression of GLP-1R in the muscle cells. The number of GLP-1R positive cells in the muscle of two groups was observed and compared. The relationship was analyzed between the homeostasis model assessment insulin resistance (HOMA-IR) and the number of GLP-1R positive cells. Results ⑴Compared to the NT group, the num-ber of GLP-1R positive cells in muscle of DM group was decreased (P<0. 05); ⑵ Compared to the NT group, HOMA-IR was increased in DM group (P<0. 05);⑶ Pearson correlation analysis showed that the percentage of GLP-1R positive cells expressed in rat skeletal muscle was negatively correlated with HOMA-IR (r= -0. 538, P<0. 01). Conclusions The expression of GLP-1R positive cells was decreased in the skeletal muscle of diabetic rats, insulin resistance increased. Therefore, the expression of GLP-1R on skele-tal muscle cells may promote the development of insulin resistance and diabetes mellitus.

Objective To analyze the impacts of two different loading methods on the sterilization of glass feeding bottle in hospitals,to confirm the best loading sterilization methods and provide the acceptable product for the clinical department.Methods By purposive sampling the glass feeding bottles sterilized between December 2013 and December 2014 in Central Sterile Supply Department,the First People's Hospital of Shangqiu,were chosen.We divided them into experimental group and control group with each 5 groups (120 bottles).The control group applied medical storage tank to load and sterilize.The experimental group sterilized through innovative stainless steel hollow with cover.Both groups were deposited in the storage rack in the clean area with air 300 000 stage air purification and temperature and humidity meeting the national standards.The professional worker supervised this two items at the same time in validity.In the period of validity,professional personnel implemented visual inspection,a magnifying glass detection and bacterial culture at the same time.Results The visualization and magnifying glass detection qualified rate of 5 packages (120 bottles) in the control group was 75.0％,sterilization quality rate 95.0％ by biological cultivation compared with 95.8％ and 100.0％ in the experimental group (P ＜ 0.05).Conclusions The usage of wrapping tool with innovative hollow stainless steel can ensure the effectiveness and the integrity of the glass feeding bottles,can reduce the medical cost and the occurrence of hospital infection.

OBJECTIVETo compare villus cell culture and karyotype analysis with single nucleotide polymorphism (SNP) microarray technology for the detection of chorionic villus chromosome in patients with retention of abortion.

METHODSForty cases were analyzed with the two methods.

RESULTSChorionic villus culturing was successful in 29 cases, among which 10 were found to have an abnormal karyotypes. For the SNP microarray analysis, all 40 cases were successful, among which 16 were shown to have an abnormal molecular karyotype.

CONCLUSIONSNP microarray technology is highly accurate and specific, which is particularly suitable for the detection of chromosomal deletions or duplications, uniparental disomy, low-percentage mosaicism and other chromosomal abnormalities. It has provided an effective supplement to the conventional chorionic villus culture and karyotype analysis.

Abortion, Missed ; genetics ; Adult ; Chorionic Villi ; chemistry ; Chromosome Aberrations ; Female ; Humans ; Karyotyping ; Male ; Oligonucleotide Array Sequence Analysis ; methods ; Polymorphism, Single Nucleotide ; Pregnancy ; Pregnancy Trimester, First ; genetics

OBJECTIVETo investigate the role of sociopsychological factors in occupational hand injury and to provide a reference for intervention measures.

METHODSThe sociopsychological factors in 150 patients with occupational hand injury who were admitted to the Department of Hand Surgery from January to June in 2014 and 150 pair-matched normal controls were surveyed using self-made questionnaire and Life Event Scale (LES). A comparative study was carried out using t test, χ² test, and conditional logistic regression analysis.

RESULTSThe demographic characteristics, such as length of service, education level, and marital status, showed significant differences between the two groups (P<0.05) ; the LES total score and values of positive life events and negative life events also showed significant differences between the study group and control group (P<0.01) ; single-factor and multi-factor conditional logistic regression analyses showed that length of service, marital status, and negative events score were statistically significant variables (P<0.01).

CONCLUSIONSociopsychological factors play an important role in the occurrence of occupational hand injury.

Case-Control Studies ; Hand Injuries ; psychology ; Hospitalization ; Humans ; Occupational Injuries ; psychology ; Surveys and Questionnaires