Objective To explore the effects of Qingre Yiqi Huayu Chinese Medicine Plaster,an hospital preparation of Shenzhen Traditional Chinese Medicine Hospital,on acute soft tissue injury in rabbits from the perspective of the apoptosis caspase-3.Methods Forty rabbits were randomly divided into blank control group,model group,Qingre Huayu Chinese Medicine Plaster group(called original prescription plaster group for short),Qingre Yiqi Huayu Chinese Medicine Plaster group[called combined(with Astragali Radix)plaster group for short],and Qingre Huayu Chinese Medicine impregnating group[called combined(with Astragali Radix)impregnating group for short],with eight rabbits in each group.Except for the blank control group,rabbits in all other groups were used to construct acute soft tissue injury model by striking method.The changes in caspase-3 expression and PGE2 level were observed in the injured tissues after 24-hour and 72-hour drug administration.Results After 24-hour drug administration,compared with the blank control group,the caspase-3 positive expression level of the three treatment groups and the model group were increased(P<0.05),among which the combined plaster group had the least expression of caspase-3 and the lowest pathological score,followed by the combined impregnating group and the original prescription plaster group.After 72-hour drug administration,the caspase-3 positive expression level of the three treatment groups was not statistically significant compared with that of the blank control group(P>0.05),while the positive expression level of caspase-3 in the model group was still higher than that of the blank control group(P<0.05).After 24-hour drug administration,the serum PGE2 level of the model group was significantly higher than that of the blank control group(P<0.05),while the difference of the serum PGE2 levels between the three treatment groups and the blank control group or the model group being not statistically significant(P>0.05);after 72-hour drug administration,the difference of the serum PGE2 level between the original prescription plaster group and the model group being not statistically significant(P>0.05),while the serum PGE2 level of the combined plaster group and combined impregnating group was lower than that of the model group,respectively(P<0.05).Conclusion All of Qingre Huayu Chinese Medicine Plaster,Qingre Yiqi Huayu Chinese Medicine Plaster,Qingre Yiqi Huayu Chinese Medicine impregnation can effectively accelerate the apoptosis of injured muscle tissue cells in rabbits with acute soft tissue injury,reduce inflammatory reaction and accelerate the repair of tissue damage,and the effect of Qingre Yiqi Huayu Chinese Medicine Plaster was superior to that of Qingre Huayu Chinese Medicine Plaster and Qingre Yiqi Huayu Chinese Medicine impregnation.

Objective To explore the clinical application of manipulations in treating cervical vertigo and the status of research on its therapeutic mechanism with bibliometric methods.Methods Using"cervical vertigo","cervicogenic dizziness","manipulations"and"tuina"as the key words,the literatures on clinical application of manipulations in treating cervical vertigo and its therapeutic mechanism were retrieved from the databases of China National Knowledge Infrastructure(CNKI),China Science and Technology Journal Database(VIP)and Wanfang Data Knowledge Service Platform,and the retrieval time was set from the establishment of each database to December 31,2023.The relevant literature obtained from the databases was screened,classified and summarized,and then statistical analysis was performed in terms of the chronological distribution,literature types and research types,therapies,and characteristics of the basic researches of the included literature.Results A total of 1 412 articles about manipulations in treating cervical vertigo and with postive outcomes have been issued in China in the recent 30 years.The quantity of literature showed an overall increasing trend before 2013,and then gradually decreased after 2013.Of the 1412 articles,1 231(87.18％)were issued by the journals,1 202(85.13％)articles were about clinical research,while only 48(3.40％)articles were about basic research.Of the 1 202 articles about clinical research,only 357 articles mentioned the name of manipulations in details definitely,which accounted for 155 types in a variety of types or names.Of the 1 202 articles about clinical research,34.44％of them were about therapy of manipulations alone for the treatment cervical vertigo,and the other clinical research were all about combined therapies,the combined therapy of manipulations plus acupuncture-moxibustion/electro-acupuncture being the most common(25.37％).Of the 48 articles about basic research,research on biomechanics was most commonly seen(38 articles,79.17％).Conclusion Manipulation therapy has been widely used for the treatment of cervical vertigo in the recent 30 years.At present,the quality of clinical research on the treatment of cervical vertigo by manipulation therapy is not high,the manipulation techniques were in a wide variety,and the research lacks of objective criterions.The research direction for basic studies is single,and breakthroughs are needed in the quality and quantity of basic research.In the future research,the naming of manipulations for cervical vertigo should be considered,and the explicit naming principles and criterions should be established;the quality level of clinical studies should be improved and the basic research should be emphasized,so as to provide more reliable theoretical and experimental evidences for manipulations in treating cervical vertigo,and promote its clinical application.

BACKGROUND:Cervical rotatory manipulation is widely used in the treatment of neck-related diseases with a clear curative effect,but it also has some risks in clinical practice.The previous study of our group found that cervical rotatory manipulation can reduce the tensile mechanical properties of the atherosclerotic carotid artery,but it is not clear about the effects of different cervical rotatory manipulations(positioning/non-positioning)and different degrees(mild/moderate/severe)of atherosclerosis on the tensile mechanical properties of the carotid artery. OBJECTIVE:To explore the effects of different cervical rotatory manipulations and different degrees of atherosclerosis on the tensile mechanical properties of the internal carotid artery. METHODS:The 120 male New Zealand rabbits were randomly divided into eight experimental groups with different degrees of atherosclerosis and different cervical rotatory manipulations:severe atherosclerosis + positioning/non-positioning cervical rotatory manipulation,moderate atherosclerosis + positioning/non-positioning cervical rotatory manipulation,mild atherosclerosis + positioning/non-positioning cervical rotatory manipulation,and normal rabbit + positioning/non-positioning cervical rotatory manipulation,as well as three model control groups:mild/moderate/severe atherosclerosis + non-cervical rotatory manipulation,and the blank control group.Two-factor analysis of variance was used to explore the main effects and interactive effects of different cervical rotatory manipulations and different degrees of atherosclerosis on the tensile mechanical properties of the internal carotid artery.One-way analysis of variance was applied to explore the influence of different cervical rotatory manipulations on the tensile mechanical properties of the internal carotid artery under the same degree of atherosclerosis. RESULTS AND CONCLUSION:(1)Both different cervical rotatory manipulations and different degrees of atherosclerosis were the main effect factors affecting the tensile mechanical properties of the internal carotid artery.(2)For both mild and severe atherosclerosis,both positioning and non-positioning cervical rotatory manipulations reduced the maximum stress of the internal carotid artery(P<0.05)and also increased the physiological elastic modulus of the internal carotid artery(P<0.05).(3)For moderate atherosclerosis,positioning and non-positioning cervical rotatory manipulations also increased the physiological elastic modulus of the internal carotid artery(P<0.05).Non-positioning cervical rotatory manipulation reduced the maximum strain of the internal carotid artery(P<0.05),and its maximum strain was also less than the internal carotid artery of the positioning cervical rotatory manipulations(P<0.05).(4)For the normal internal carotid artery,in addition to the maximum strain,both positioning and non-positioning cervical rotatory manipulations had no statistically significant effects on other tensile mechanical indicators of the internal carotid artery(P>0.05).(5)The results suggest that both positioning and non-positioning cervical rotatory manipulations may increase the stiffness of the atherosclerotic internal carotid artery,reducing its elasticity and brittleness.Therefore,both positioning and non-positioning cervical rotatory manipulations may increase the risk of cardiovascular events in mild/moderate/severe atherosclerotic internal carotid artery,and the more severe the atherosclerosis is,the greater the risk of positioning/non-positioning cervical rotatory manipulation treatment,but the risk of positioning cervical rotatory manipulation is not lower than that of non-positioning cervical rotatory manipulation.