Background and aims:Promoting gastrointestinal function recovery is one of the key aspects of enhanced recovery after surgery(ERAS)for colorectal cancer(CRC).Many ancient Chinese medical texts suggest that acupuncture at four acupoints—Zusanli,Shangjuxu,Sanyinjiao,and Neiguan—helps promote gastrointestinal peristalsis.However,there is currently no systematic study on multi-point acupuncture to promote postoperative gastrointestinal function recovery in CRC patients.Therefore,this study was conducted to evaluate the clinical efficacy of multi-point acupuncture in promoting gastrointestinal function recovery in CRC patients through a prospective study to provide new options for implementing ERAS in CRC patients in clinical practice. Methods:Using a prospective,randomized,controlled design,a total of 100 eligible patients who underwent laparoscopic CRC radical surgery at the Department of Gastrointestinal Surgery,Third Xiangya Hospital of Central South University,between January 2021 and February 2022,were randomly divided into an observation group and a control group,with 50 patients in each group.Both groups underwent the same ERAS protocols and radical surgery during the perioperative period.The observation group received acupuncture treatment from the day of surgery to the third postoperative day,once per day,while the control group did not receive acupuncture.Primary outcomes included the time to bowel sound recovery,time to first flatus,and time to first defecation.Secondary outcomes included abdominal pain scores,incidence of bloating,nausea,vomiting,white blood cell count,neutrophil percentage,C-reactive protein,hemoglobin,serum albumin,blood potassium levels,postoperative hospital stay,and incidence of postoperative complications. Results:A total of 96 patients completed the study,with 48 in each group.There were no statistically significant differences in baseline characteristics such as age,gender,or surgery time between the two groups(all P＞0.05).Compared with the control group,the observation group had a significantly shorter time for bowel sound recovery,first flatus,first defecation,and postoperative hospital stay(all P＜0.05).Additionally,the observation group showed reduced abdominal pain scores on postoperative day(POD)2 and 3,a lower incidence of bloating on POD 1 and 2,and a reduced incidence of nausea and vomiting on POD 3(all P＜0.05).There were no statistically significant differences in the remaining comparisons between the two groups(all P＞0.05). Conclusion:Multi-point acupuncture treatment can promote the recovery of gastrointestinal function in patients undergoing minimally invasive CRC surgery without increasing postoperative complications,and it is recommended for integration into routine ERAS protocols.

Objective:To investigate the functional connectivity of default mode network (DMN) and limbic system, the expression level of inflammatory cytokine and their correlation in bipolar disorder type Ⅱ(BDⅡ) patients with depressive episodes.Methods:Thirty-three BD Ⅱ patients with depressive episodes and forty-six healthy controls were recruited to complete the resting-state functional magnetic resonance imaging (rs-fMRI). After image preprocessing, the DMN and limbic system were extracted from the image data by independent component analysis (ICA), so as to compare the differences of functional connectivity of resting brain network between the patients and the controls.Serum levels of inflammatory cytokines interleukin-6 (IL-6), interleukin-8(IL-8), interleukin-10(IL-10), tumor necrosis factor-α (TNF-α), and C-C motif chemokine ligand 4 (CCL4) in patients and healthy controls were detected.The correlation between functional connectivity of different brain regions and inflammatory cytokines was analyzed.SPSS 17.0 software was used for data statistical analysis.The two samples were compared using t-test or Mann-Whitney U-test, and Spearman was used for correlation testing. Results:In BDⅡ patients, the functional connectivity of the right medial prefrontal cortex(cluster-size=7 voxel, cluster-level PGRF<0.05, MNI: x=6, y=54, z=9, t=-3.765) and the left superior frontal gyrus(cluster-size=10 voxel, cluster-level PGRF<0.05, MNI: x=-21, y=54, z=15, t=-4.139) in DMN decreased, while the left cerebellum Ⅳ and Ⅴ lobules of limbic system (cluster-size=21 voxel, cluster-level PGRF<0.05, MNI: x=-15, y=-24, z=-30, t=4.468) and cerebellar tonsil of left cerebellum posterior lobe(cluster-size=8 voxel, cluster-level PGRF<0.05, MNI: x=-15, y=-51, z=-45, t=4.138) in the limbic system increased.Compared with the healthy controls, the serum levels of IL-10(7.39 (6.33, 9.32) pg/mL vs 6.54 (5.84, 7.39) pg/mL, Z=-2.937, P=0.003)and CCL4 (39.31 (25.77, 68.70) pg/mL vs 31.30 (20.32, 40.89) pg/mL, Z=-2.209, P=0.027) were higher in BDⅡ patients.The functional connectivity of the left cerebellum Ⅳ and Ⅴ lobules was positively correlated with the serum levels of IL-10 ( r=0.432, P=0.031) and that of the cerebellar tonsil of left cerebellum posterior lobe was positively correlated with the serum levels of IL-10 ( r=0.429, P=0.032) and CCL4 ( r=0.402, P=0.046). Conclusion:The functional connectivity of DMN and limbic system in BDⅡ patients with depressive episode is abnormal in resting-state fMRI.The expression level of inflammatory cytokines in patients' serum increases, and has correlation with the functional connection of limbic system.

Objective:To analyze the molecular epidemiological characteristics of the Corona virus disease 2019 (COVID-19) cases in Shijiazhuang, which can reveal the origin of the outbreak and provide a scientific basis for COVID-19 prevention and control.Methods:From January 2 to January 8, 2021, a total of 404 samples from 170 COVID-19 cases were collected from the Shijiazhuang Fifth Hospital. The consensus sequence of 2019 novel Coronavirus(2019-nCoV) was obtained through multiplex polymerase chain reaction-based sequencing. The sequences of 170 COVID-19 cases were analyzed by the PANGOLIN, and the data were statistically analyzed by T-test.Results:Among the 404 COVID-19 samples, a total of 356 samples obtained high quality genome sequences (>95%,100×sequencing depth). The whole genome sequences of 170 COVID-19 cases were obtained by eliminating repeated samples. All 170 sequences were recognized as lineage B1.1 using PANGOLIN. The number of single nucleotide polymorphism arrange from 18-22 and most of the single nucleotide polymorphism were synonymous variants. All of 170 genomes could be classified into 48 sub-groups and most of the genomes were classified into 2 sub-groups (66 and 31, respectively).Conclusions:All cases in this study are likely originated from one imported case. The viruses have spread in the community for a long time and have mutated during the community transmission.

Objective:To compare the changes of cerebral blood flow (CBF) in bipolar disorder type Ⅱ patients with and without suicidal ideation.Methods:Pseudo-continuous arterial spin labelling (pCASL) was examined with resting-state functional magnetic resonance imaging(rs-fMRI) on all subjects, CBF images of bipolar disorder type Ⅱ depression with suicidal ideation ( n=48), Bipolar Disorder type Ⅱ without suicidal ideation ( n=41), healthy controls ( n=62) at rest were collected. One-way ANOVA and Gaussian random field (GRF) were used to compare the CBF values between the groups to locate the regions with significant change. Results:Compared with the control group, CBF of left superior temporal gyrus (extending to the temporal pole) ( t=3.87, P<0.01; t=4.44, P<0.01) and left putamen ( t=4.04, P<0.01; t=3.19, P<0.01) increased in bipolar disorder type Ⅱ patients with and without suicidal ideation, and CBF of the right posterior cingulate gyrus (extending to the calcarine gyrus) decreased in bipolar disorder type Ⅱ patients with suicidal ideation( t=-4.66, P<0.01). Conclusions:There were abnormal CBF in left superior temporal gyrus and left putamen in bipolar disorder type Ⅱ patients with suicidal ideation and bipolar disorder type Ⅱ patients without suicidal ideation, while the decreased CBF in right posterior cingulate gyrus may be the specific brain change in bipolar disorder type Ⅱ patients with suicidal ideation.

Objective:To compare the changes of cerebral blood flow (CBF) in bipolar disorder type Ⅱ patients with and without suicidal ideation.Methods:Pseudo-continuous arterial spin labelling (pCASL) was examined with resting-state functional magnetic resonance imaging(rs-fMRI) on all subjects, CBF images of bipolar disorder type Ⅱ depression with suicidal ideation ( n=48), Bipolar Disorder type Ⅱ without suicidal ideation ( n=41), healthy controls ( n=62) at rest were collected. One-way ANOVA and Gaussian random field (GRF) were used to compare the CBF values between the groups to locate the regions with significant change. Results:Compared with the control group, CBF of left superior temporal gyrus (extending to the temporal pole) ( t=3.87, P<0.01; t=4.44, P<0.01) and left putamen ( t=4.04, P<0.01; t=3.19, P<0.01) increased in bipolar disorder type Ⅱ patients with and without suicidal ideation, and CBF of the right posterior cingulate gyrus (extending to the calcarine gyrus) decreased in bipolar disorder type Ⅱ patients with suicidal ideation( t=-4.66, P<0.01). Conclusions:There were abnormal CBF in left superior temporal gyrus and left putamen in bipolar disorder type Ⅱ patients with suicidal ideation and bipolar disorder type Ⅱ patients without suicidal ideation, while the decreased CBF in right posterior cingulate gyrus may be the specific brain change in bipolar disorder type Ⅱ patients with suicidal ideation.

Objective To provide a basic profile of resting?state functional connectivity (FC) in unmedicated patients with major depressive disorder (MDD). Methods Fifty unmedicated patients with MDD and 51 healthy controls underwent the resting?state functional magnetic resonance imaging (rs?fMRI). After preprocessing of rs?fMRI, the seed?based resting?state FC of the insular subdivisions, including the anterior insula, middle insula, and posterior insula, was evaluated. Two?sample t?test and multiple comparison correction of Threshold?Free Cluster Enhancement (TFCE) were used to compare the FC values of each group to locate the regions with significant change, where its correlation to the Hamilton Depression Scale (HAMD24) scores was also explored. Results In comparison to the healthy controls, the MDD patients decreased FC between the left anterior insula and bilateral anterior cingulate cortices (t=-4.83, P<0.05, TFCE correction), and increased FC between the left anterior insula and the left middle frontal gyrus, as well as the bilateral posterior cingulate cortices/precuneus (t=4.08, 4.42;both P<0.05, TFCE correction). There were no significant correlations between the HAMD24 scores and the FC values from the left anterior insula to the bilateral anterior cingulate cortices (r=-0.125, P=0.387), the left middle frontal gyrus (r=0.149, P=0.302), and the bilateral posterior cingulate cortices/precuneus (r=-0.207, P=0.148). Conclusion Patients with depression have abnormal functional connectivity between the left anterior insula and the other two regions, such as the limbic system and the frontal cortex, which may present with an aweakened insula?anterior cingulate gyrus connectivity, and the enhanced insular?frontal, and insula-posterior cingulate/precuneus connectivity.

Objective To investigate the effects of ganglioside combined with hyperbaric oxygen (HBO) on cognitive dysfunction and brain-derived neurotrophic factors (BDNF) in patients with cerebral hemorrhage after surgery.Methods One hundred and twenty patients with cerebral hemorrhage hospitalized in the Neurology Department of the hospital from April 2014 to December 2018 were enrolled for the study,and were randomly divided into the control group and the observation group,each consisting of 60 patients.The patients of the control group were given ganglioside treatment while the patients of the observation group received HBO therapy in addition to the treatment received by the control group.After 3 courses of treatment,changes in serum BDNF levels before and after treatment were compared between the 2 groups.Cognitive impairment was assessed by using the Mini-Mental State Examination (MMSE) Scale.The scores of neurological function was assessed by using the National Institute of Health Stroke Scale (NIHSS) and Activities of Daily Life(ADL).Clinical curative effects were assessed in accordance with relevant criteria and adverse reactions were observed in the patients of the 2 groups.Results After 3 courses of treatment,the total efficacy rate of the observation group was higher than that of the control group(P ＜ 0.05).The MMSE scores of the 2 groups after treatment were significantly increased,as compared with those before treatment,and the scores of the observation group were obviously higher than those of the control group,and statistical significance could be noted,when comparisons were made between the 2 groups (P ＜ 0.05 or P ＜ 0.01).After treatment,the NIHSS scores of the 2 groups all elevated as compared with the scores before treatment,and the scores of the observation group were obviously lower than those of the control group (P ＜ 0.05 or P ＜ 0.01).Following treatment,the ADL scores of the 2 groups all elevated significantly as compared with the scores before treatment,and the scores of the observation group were obviously higher than those of the control group (P ＜ 0.05).After treatment,serum BDNF levels of the 2 groups all increased as compared with those before treatment,and the levels of the observation group were obviously higher than those of the control group.Statistical significance could be seen,when comparisons were made between the 2 groups(P ＜ 0.05).However,there was no statistical significance in the rate of adverse reactions,when comparisons were made between the 2 groups(P ＞ 0.05).Conclusion HBO combined with ganglioside could effectively promote recovery of cognitive function of the patients with cerebral hemorrhage after surgery,in the meantime it could increase serum BDNF level,with good safety.For this reason,it is worth further clinical extension.

Objective To investigate the characteristics of striatum functional connectivity (FC) in patients with major depressive disorder (MDD).Methods Fifty unmedicated patients with MDD and 48 healthy controls underwent resting-state functional magnetic resonance imaging (rs-fMRI) scan.Routine preprocessing of rs-fMRI data was performed.The striatum was divided into bilateral inferior ventral striatum,bilateral superior ventral striatum,bilateral dorsal caudate,bilateral dorsal caudal putamen,bilateral dorsal rostral putamen,and bilateral ventral rostral putamen.Whole brain functional connectivity analysis was performed with the above 12 seed points.And significant differences of each seed FC among two groups were calculated with Two-sample t-test and multiple comparison correction of threshold-free cluster enhancement (TFCE).Finally,correlation analysis was performed between the FC of the brain regions and clinical features (i.e.Hamilton Depression Scale of 24 items (HAMD24) scores).Results In comparison to the control group,the MDD patients showed significantly decreased FC in the left dorsal caudal putamen and the right superior temporal gyrus (t=-5.46,P＜0.008,TFCE correction),the right dorsal rostral putamen and bilateral postcentral gyrus (right t=-4.85,left t=-4.81,P＜0.008,TFCE correction) and left precuneus (t=-4.58,P＜0.008,TFCE correction).There were no significant correlations between the FC values in these regions and HAMD24 scores (r=0.171,0.002,0.005,0.098;all P＜0.05).Conclusion The MDD patients possibly have abnormal FC in the striatum and the superior temporal gyrus,the postcentral gyrus and the precuneus,and it suggests these sensorimoter areas may play an important role in the pathological mechanisms of MDD.

Objective To provide a basic profile of resting?state functional connectivity (FC) in unmedicated patients with major depressive disorder (MDD). Methods Fifty unmedicated patients with MDD and 51 healthy controls underwent the resting?state functional magnetic resonance imaging (rs?fMRI). After preprocessing of rs?fMRI, the seed?based resting?state FC of the insular subdivisions, including the anterior insula, middle insula, and posterior insula, was evaluated. Two?sample t?test and multiple comparison correction of Threshold?Free Cluster Enhancement (TFCE) were used to compare the FC values of each group to locate the regions with significant change, where its correlation to the Hamilton Depression Scale (HAMD24) scores was also explored. Results In comparison to the healthy controls, the MDD patients decreased FC between the left anterior insula and bilateral anterior cingulate cortices (t=-4.83, P<0.05, TFCE correction), and increased FC between the left anterior insula and the left middle frontal gyrus, as well as the bilateral posterior cingulate cortices/precuneus (t=4.08, 4.42;both P<0.05, TFCE correction). There were no significant correlations between the HAMD24 scores and the FC values from the left anterior insula to the bilateral anterior cingulate cortices (r=-0.125, P=0.387), the left middle frontal gyrus (r=0.149, P=0.302), and the bilateral posterior cingulate cortices/precuneus (r=-0.207, P=0.148). Conclusion Patients with depression have abnormal functional connectivity between the left anterior insula and the other two regions, such as the limbic system and the frontal cortex, which may present with an aweakened insula?anterior cingulate gyrus connectivity, and the enhanced insular?frontal, and insula-posterior cingulate/precuneus connectivity.

Objective To investigate the effects of ganglioside combined with hyperbaric oxygen (HBO) on cognitive dysfunction and brain-derived neurotrophic factors (BDNF) in patients with cerebral hemorrhage after surgery.Methods One hundred and twenty patients with cerebral hemorrhage hospitalized in the Neurology Department of the hospital from April 2014 to December 2018 were enrolled for the study,and were randomly divided into the control group and the observation group,each consisting of 60 patients.The patients of the control group were given ganglioside treatment while the patients of the observation group received HBO therapy in addition to the treatment received by the control group.After 3 courses of treatment,changes in serum BDNF levels before and after treatment were compared between the 2 groups.Cognitive impairment was assessed by using the Mini-Mental State Examination (MMSE) Scale.The scores of neurological function was assessed by using the National Institute of Health Stroke Scale (NIHSS) and Activities of Daily Life(ADL).Clinical curative effects were assessed in accordance with relevant criteria and adverse reactions were observed in the patients of the 2 groups.Results After 3 courses of treatment,the total efficacy rate of the observation group was higher than that of the control group(P ＜ 0.05).The MMSE scores of the 2 groups after treatment were significantly increased,as compared with those before treatment,and the scores of the observation group were obviously higher than those of the control group,and statistical significance could be noted,when comparisons were made between the 2 groups (P ＜ 0.05 or P ＜ 0.01).After treatment,the NIHSS scores of the 2 groups all elevated as compared with the scores before treatment,and the scores of the observation group were obviously lower than those of the control group (P ＜ 0.05 or P ＜ 0.01).Following treatment,the ADL scores of the 2 groups all elevated significantly as compared with the scores before treatment,and the scores of the observation group were obviously higher than those of the control group (P ＜ 0.05).After treatment,serum BDNF levels of the 2 groups all increased as compared with those before treatment,and the levels of the observation group were obviously higher than those of the control group.Statistical significance could be seen,when comparisons were made between the 2 groups(P ＜ 0.05).However,there was no statistical significance in the rate of adverse reactions,when comparisons were made between the 2 groups(P ＞ 0.05).Conclusion HBO combined with ganglioside could effectively promote recovery of cognitive function of the patients with cerebral hemorrhage after surgery,in the meantime it could increase serum BDNF level,with good safety.For this reason,it is worth further clinical extension.