Objective:To investigate the clinicopathological features, genetic characteristics, and differential diagnosis of glomangiomatosis with uncertain malignant potential.Methods:Two cases of glomangiomatosis with uncertain malignant potential were collected at Henan Provincial People′s Hospital from 2013 and 2023. Immunohistochemistry and next generation sequencing (DNA-seq) were used to detect the related protein and gene variation. Patients were followed up.Results:Case 1 was male, 34 years old; and case 2 was female, 28 years old. Both had tumor recurrence in the original site. There were multiple nodules at right calf and ankle, involving superficial subcutaneous tissue and deep interfascicular muscles; some nodules were borderless and painful. Microscopically, the tumor was nodular with fibrous pseudocapsule, some had indistinct borders and diffuse infiltration to the surrounding adipose tissue. The tumor cells were round to ovoid with inconspicuous nucleoli, partly surrounding small irregularly dilated thin-walled blood vessels. The recurrent tumors showed epithelioid morphology in some of the tumor cells, with eosinophilic cytoplasm, some apparent nucleoli, mild to moderate nuclear atypia, and brisk mitotic figures. Focally, perimuscular cell differentiation was noted. The small lesion showed intravascular tumor thrombus. NGS revealed BRAF V600E mutation in case 1, and BRAF V600E mutation combined with PDGFRB gene amplification in case 2.Conclusions:Glomangiomatosis with uncertain malignant potential is a rare variant of glomus tumor. It has a unique growth pattern morphologically, BRAF V600E mutation, and invasive biological behavior.

BACKGROUND:Bone marrow mesenchymal stem cells in patients with osteoporosis show significant senescence and decreased activity and osteogenic differentiation.miR-212-3p inhibits osteogenic differentiation of human bone marrow mesenchymal stem cells.However,its regulation of senescence of bone marrow mesenchymal stem cells and its mechanism remain unclear.OBJECTIVE:To investigate the effect of miR-212-3p on senescence of bone marrow mesenchymal stem cells by targeting mitogen-activated protein kinase 3 (MAPK3) and its mechanism.METHODS:Rat bone marrow mesenchymal stem cells were isolated and cultured in vitro,and the third generation was collected for the following experiments:(1) Cultured in two groups:The control group was added with complete culture medium,and the model group was added with complete culture medium containing H2O2.After 72 hours of culture,β-galactosidase activity,miR-212-3p and MAPK3 mRNA expression,as well as MAPK3,p16,and p21 protein expression were detected.(2) Cultured in three groups:control group,inhibitor control group,and miR-212-3p inhibitor group.After transfection for 24 hours,miR-212-3p,mRNA and protein expression of MAPK3 were detected.(3) Dual luciferase reporter gene combined with qRT-PCR and western blot assay were used to verify the targeting regulation of miR-212-3p and MAPK3.(4) Cultured in different groups:control inhibitor group,miR-212-3p inhibitor group,miR-212-3p inhibitor+interference control group,and miR-212-3p inhibitor+MAPK3 interference group.After transfection for 24 hours,MAPK protein and mRNA expression levels in cells were detected.They were divided into control group,H2O2 group,H2O2+control inhibitor group,H2O2+miR-212-3p inhibitor group,H2O2+miR-212-3p inhibitor+interference control group,and H2O2+miR-212-3p inhibitor+MAPK3 interference group.Cells were transfected for 24 hours and then cultured with H2O2 for 72 hours.Aging-related β-galactosidase activity and p16 and p21 protein expression were detected.RESULTS AND CONCLUSION:(1) Compared with the control group,β-galactosidase activity,miR-212-3p mRNA expression and p16,p21 protein expression were increased in the model group (P<0.05),while MAPK3 mRNA and protein expression levels were decreased (P<0.05).(2) Compared with the control group,the mRNA expression of miR-212-3p was decreased (P<0.05),and the mRNA and protein expression levels of MAPK3 were increased (P<0.05) in miR-212-3p inhibitor group.(3) Double luciferase reporter gene experiment confirmed that MAPK3 was the downstream target gene of miR-212-3p.(4) Compared with the control inhibitor group,the mRNA and protein expression levels of MAPK3 were increased in miR-212-3p inhibitor group (P<0.05).Compared with the miR-212-3p inhibitor group,the mRNA and protein expression levels of MAPK3 in the miR-212-3p inhibitor+MAPK3 interference group were decreased (P<0.05).Compared with H2O2+control inhibitor group,β-galactosidase activity in H2O2+miR-212-3p inhibitor group was decreased (P<0.05).Compared with H2O2+miR-212-3p inhibitor group,β-galactosidase activity in H2O2+miR-212-3p inhibitor+MAPK3 interference group was higher than that in H2O2+miR-212-3p inhibitor group (P<0.05).Compared with the H2O2+control inhibitor group,the protein expression levels of p16 and p21 in the H2O2+miR-212-3p inhibitor group were decreased (P<0.05).Compared with H2O2+miR-212-3p inhibitor group,the protein expression levels of p16 and p21 in H2O2+miR-212-3p inhibitor+MAPK3 interference group were increased (P<0.05).(5) To conclude,downregulation of miR-212-3p inhibits the senescence of rat bone marrow mesenchymal stem cells,and its mechanism of action may be achieved by targeting up-regulation of MAPK3 expression.

BACKGROUND:Bone marrow mesenchymal stem cells in patients with osteoporosis show significant senescence and decreased activity and osteogenic differentiation.miR-212-3p inhibits osteogenic differentiation of human bone marrow mesenchymal stem cells.However,its regulation of senescence of bone marrow mesenchymal stem cells and its mechanism remain unclear.OBJECTIVE:To investigate the effect of miR-212-3p on senescence of bone marrow mesenchymal stem cells by targeting mitogen-activated protein kinase 3 (MAPK3) and its mechanism.METHODS:Rat bone marrow mesenchymal stem cells were isolated and cultured in vitro,and the third generation was collected for the following experiments:(1) Cultured in two groups:The control group was added with complete culture medium,and the model group was added with complete culture medium containing H2O2.After 72 hours of culture,β-galactosidase activity,miR-212-3p and MAPK3 mRNA expression,as well as MAPK3,p16,and p21 protein expression were detected.(2) Cultured in three groups:control group,inhibitor control group,and miR-212-3p inhibitor group.After transfection for 24 hours,miR-212-3p,mRNA and protein expression of MAPK3 were detected.(3) Dual luciferase reporter gene combined with qRT-PCR and western blot assay were used to verify the targeting regulation of miR-212-3p and MAPK3.(4) Cultured in different groups:control inhibitor group,miR-212-3p inhibitor group,miR-212-3p inhibitor+interference control group,and miR-212-3p inhibitor+MAPK3 interference group.After transfection for 24 hours,MAPK protein and mRNA expression levels in cells were detected.They were divided into control group,H2O2 group,H2O2+control inhibitor group,H2O2+miR-212-3p inhibitor group,H2O2+miR-212-3p inhibitor+interference control group,and H2O2+miR-212-3p inhibitor+MAPK3 interference group.Cells were transfected for 24 hours and then cultured with H2O2 for 72 hours.Aging-related β-galactosidase activity and p16 and p21 protein expression were detected.RESULTS AND CONCLUSION:(1) Compared with the control group,β-galactosidase activity,miR-212-3p mRNA expression and p16,p21 protein expression were increased in the model group (P<0.05),while MAPK3 mRNA and protein expression levels were decreased (P<0.05).(2) Compared with the control group,the mRNA expression of miR-212-3p was decreased (P<0.05),and the mRNA and protein expression levels of MAPK3 were increased (P<0.05) in miR-212-3p inhibitor group.(3) Double luciferase reporter gene experiment confirmed that MAPK3 was the downstream target gene of miR-212-3p.(4) Compared with the control inhibitor group,the mRNA and protein expression levels of MAPK3 were increased in miR-212-3p inhibitor group (P<0.05).Compared with the miR-212-3p inhibitor group,the mRNA and protein expression levels of MAPK3 in the miR-212-3p inhibitor+MAPK3 interference group were decreased (P<0.05).Compared with H2O2+control inhibitor group,β-galactosidase activity in H2O2+miR-212-3p inhibitor group was decreased (P<0.05).Compared with H2O2+miR-212-3p inhibitor group,β-galactosidase activity in H2O2+miR-212-3p inhibitor+MAPK3 interference group was higher than that in H2O2+miR-212-3p inhibitor group (P<0.05).Compared with the H2O2+control inhibitor group,the protein expression levels of p16 and p21 in the H2O2+miR-212-3p inhibitor group were decreased (P<0.05).Compared with H2O2+miR-212-3p inhibitor group,the protein expression levels of p16 and p21 in H2O2+miR-212-3p inhibitor+MAPK3 interference group were increased (P<0.05).(5) To conclude,downregulation of miR-212-3p inhibits the senescence of rat bone marrow mesenchymal stem cells,and its mechanism of action may be achieved by targeting up-regulation of MAPK3 expression.

Objective:To investigate the clinicopathological features, genetic characteristics, and differential diagnosis of glomangiomatosis with uncertain malignant potential.Methods:Two cases of glomangiomatosis with uncertain malignant potential were collected at Henan Provincial People′s Hospital from 2013 and 2023. Immunohistochemistry and next generation sequencing (DNA-seq) were used to detect the related protein and gene variation. Patients were followed up.Results:Case 1 was male, 34 years old; and case 2 was female, 28 years old. Both had tumor recurrence in the original site. There were multiple nodules at right calf and ankle, involving superficial subcutaneous tissue and deep interfascicular muscles; some nodules were borderless and painful. Microscopically, the tumor was nodular with fibrous pseudocapsule, some had indistinct borders and diffuse infiltration to the surrounding adipose tissue. The tumor cells were round to ovoid with inconspicuous nucleoli, partly surrounding small irregularly dilated thin-walled blood vessels. The recurrent tumors showed epithelioid morphology in some of the tumor cells, with eosinophilic cytoplasm, some apparent nucleoli, mild to moderate nuclear atypia, and brisk mitotic figures. Focally, perimuscular cell differentiation was noted. The small lesion showed intravascular tumor thrombus. NGS revealed BRAF V600E mutation in case 1, and BRAF V600E mutation combined with PDGFRB gene amplification in case 2.Conclusions:Glomangiomatosis with uncertain malignant potential is a rare variant of glomus tumor. It has a unique growth pattern morphologically, BRAF V600E mutation, and invasive biological behavior.

Objective To analyze the genetic characteristics of measles virus in Sichuan province in 2015.Methods Measles virus was isolated from throat swab specimens collected from suspected measles cases.Nucleotide sequences coding for C terminus of nucleoprotein were amplified by RT-PCR and sequenced for phylogenetic analysis.Results 398 measles virus isolates were obtained from 971 throat swab specimens.Phylogenetic analysis showed that 397 belonged to H1 genotype,the sequences homologies were 96.2％-98.0％ compared with H1 genotype reference strain;and all those isolates separated into 2 clusters in phylogenetic tree with the average nucleotide divergence of 2.0％.1 isolate belonged to A genotype,and shared 98.0％ nucleotide homology compared with A genotype reference strain.Conclusions H1 genotype virus remained predominant circulating in Sichuan Province.There was no much genetic variation in N gene.But a minor nucleotide divergence existed between different clusters,leading to 2 transmission chains.

Through integration of medical imaging resources of 12 community hospitals in the region, a community hospital diagnostic image center was established in 6th hospital. This paper describes the design of the structure and mode of data storage of regional PACS system and the establishment of a database of medical imaging, through which the medical imaging diagnostic level in the community was improved significantly. Through the application of regional PACS system, medical resource was saved and patient treatment facilitated.
Computer Communication Networks ; Information Storage and Retrieval ; methods ; Radiology Information Systems ; Software Design

Through integration of medical imaging resources of 12 community hospitals in the region, a community hospital diagnostic image center was established in 6th hospital. This paper describes the design of the structure and mode of data storage of regional PACS system and the establishment of a database of medical imaging, through which the medical imaging diagnostic level in the community was improved significantly. Through the application of regional PACS system, medical resource was saved and patient treatment facilitated.

This paper introduces a new application of PACS in our hospital. Through the integration of PACS, HIS and RIS, digital transformation is made in every step. The functional modules of Body Parts Examined in DICOM is set and good link between PACS and DR is made. So the equipment can retrieval the inspection area automatically and make adjustment on the parameters correspondingly. It makes the workflow optimized and improves the efficiency greatly.
Automatic Data Processing ; instrumentation ; methods ; Radiology Information Systems

BACKGROUNDThe gene expressions of the two primary cell types with different morphological phenotypes,namely,cuboidal cells and polygonal cells in so-called pulmonary sclerosing hemangioma(PSH) were still unclear now.Disclosing the different gene expression of the two cell types will provide the basis for the study of histogenesis of PSH tissue and be helpful for differential diagnosis.

METHODSPolygonal and cuboidal cells were obtained from PSH tissue using a laser capture microdissection(LCM) technique.Total RNA was extracted and mRNA levels of cytokeratin(CK),epithelial membrane antigen(EMA),vimentin(VT),surfactant protein B(SP-B),thyroid transcription factor-1(TTF-1),synaptophysin(Syn),and chromogranin A(Ch-A) were analyzed by RT-PCR.

RESULTSThe CK,EMA and SP-B genes were clearly expressed in cuboidal cells,while the VT and Syn genes were clearly expressed and the EMA gene was weakly expressed in polygonal cells.TTF-1 was expressed in both cell types,while neither cell type expressed ChA.

CONCLUSIONSThe differences in morphological phenotype might result from differences in the state of differentiation,cuboidal cells may differentiate into type II pneumocytes,while polygonal cells in stroma possess the multipotency differentiation.