[Objective] To analyze the infection status of hepatitis E virus (HEV) among blood donors in Zhengzhou, so as to provide data support for formulating local blood screening strategies. [Methods] Random samples from blood donors from January to December 2022 were tested for HEV RNA using PCR technology. Reactive samples were sequenced for gene analysis, and the donors were followed up. [Results] Among 21 311 samples, 3(0.14‰) were reactive for HEV RNA, all of whom were male. Genetic sequencing results revealed that one strong positive sample was genotype 4, while sequencing failed for the other two due to low viral load. A follow-up of 25 strong positive donors showed that ALT significantly increased on day 7 after donation, anti-HEV IgM and anti-HEV IgG turned positive. On day 21, ALT returned to normal, and on day 35, HEV RNA turned negative. Notably, anti-HEV IgM and anti-HEV IgG persisted until day 482. [Conclusion] There is HEV infection among blood donors in Zhengzhou, and it is necessary to expand the screening scope to comprehensively explore the prevalence and genotype distribution of HEV among blood donors.

Objective:To investigate the effects of dagliprazin combined with olmesartan ester on the expression of Plin and miR-26a in renal tissues of diabetic nephropathy rats.Methods:Sixty male SD rats were selected and fed adaptively for 1 week, then fed high-fat and high-sugar diet for 2 months, and then injected streptozotocin (STZ) 30 mg/kg for modeling. They were given 10 mg/kg daaglizin intragastric administration as daglizin group, 10 mg/kg olmesartan ester intragastric administration as olmesartan ester group, 5 mg/kg daaglizin and 5 mg/kg olmesartan ester intragastric administration as daglizin and olmesartan ester combination group, respectively. Twenty rats were given 10 mg/kg normal saline intragastric administration as normal control group. After 3 months, the serum samples of rats were taken to detect the levels of fasting blood glucose, blood creatinine, urea nitrogen, 24h urinary microalbumin and other renal function indicators and the levels of C-reactive protein (CRP), tumor necrosis factor (TNF-α), cell surface specific marker antigen (ED-1) and other inflammatory responses. Then the rats were killed and their kidney tissues were taken. The protein and gene expression of vascular endothelial growth factor (VEGF), perilipin (Plin), Nephlrin, miR-26a were detected by Western blot and real-time fluorescence quantitative PCR.Results:The levels of fasting blood glucose, urea nitrogen, creatinine and 24h urinary microalbumin in dagliprazin group, olmesartan ester group and combined group were higher than those in normal control group ( P<0.05), while the levels of fasting blood glucose, urea nitrogen, creatinine and 24h urinary microalbumin in combined group were lower than those in dagliprazin group and olmesartan ester group ( P<0.05). The difference between groups was statistically significant ( P<0.05). The levels of serum CRP, TNF-αand ED-1 in dagliprazin group, olmesartan ester group and combined group were higher than those in normal control group ( P<0.05), while the levels of CRP, TNF-αand ED-1 in combined group were lower than those in dagliprazin group and olmesartan ester group ( P<0.05), and the differences between groups were statistically significant ( P<0.05). The expression of serum VEGF, Plin and Nephlrin protein in dagliprazin group, olmesartan ester group and combined group were lower than those in normal control group ( P<0.05), while the protein and gene of Plin and Nephlrin in combined group were lower than those in dagliprazin group and olmesartan ester group. The mRNA and protein expressions of miR-26a and VEGF were higher than those of Daglipzin group and olmesartan ester group ( P<0.05), and the differences between groups were statistically significant ( P<0.05) . Conclusion:Dagliprazin combined with olmesartan ester can reduce kidney injury and delay the progression of diabetic nephropathy by regulating the expression of Plin and miR-26a protein and gene in renal tissue of diabetic nephropathy rats.

Objective:To investigate the effects of dagliprazin combined with olmesartan ester on the expression of Plin and miR-26a in renal tissues of diabetic nephropathy rats.Methods:Sixty male SD rats were selected and fed adaptively for 1 week, then fed high-fat and high-sugar diet for 2 months, and then injected streptozotocin (STZ) 30 mg/kg for modeling. They were given 10 mg/kg daaglizin intragastric administration as daglizin group, 10 mg/kg olmesartan ester intragastric administration as olmesartan ester group, 5 mg/kg daaglizin and 5 mg/kg olmesartan ester intragastric administration as daglizin and olmesartan ester combination group, respectively. Twenty rats were given 10 mg/kg normal saline intragastric administration as normal control group. After 3 months, the serum samples of rats were taken to detect the levels of fasting blood glucose, blood creatinine, urea nitrogen, 24h urinary microalbumin and other renal function indicators and the levels of C-reactive protein (CRP), tumor necrosis factor (TNF-α), cell surface specific marker antigen (ED-1) and other inflammatory responses. Then the rats were killed and their kidney tissues were taken. The protein and gene expression of vascular endothelial growth factor (VEGF), perilipin (Plin), Nephlrin, miR-26a were detected by Western blot and real-time fluorescence quantitative PCR.Results:The levels of fasting blood glucose, urea nitrogen, creatinine and 24h urinary microalbumin in dagliprazin group, olmesartan ester group and combined group were higher than those in normal control group ( P<0.05), while the levels of fasting blood glucose, urea nitrogen, creatinine and 24h urinary microalbumin in combined group were lower than those in dagliprazin group and olmesartan ester group ( P<0.05). The difference between groups was statistically significant ( P<0.05). The levels of serum CRP, TNF-αand ED-1 in dagliprazin group, olmesartan ester group and combined group were higher than those in normal control group ( P<0.05), while the levels of CRP, TNF-αand ED-1 in combined group were lower than those in dagliprazin group and olmesartan ester group ( P<0.05), and the differences between groups were statistically significant ( P<0.05). The expression of serum VEGF, Plin and Nephlrin protein in dagliprazin group, olmesartan ester group and combined group were lower than those in normal control group ( P<0.05), while the protein and gene of Plin and Nephlrin in combined group were lower than those in dagliprazin group and olmesartan ester group. The mRNA and protein expressions of miR-26a and VEGF were higher than those of Daglipzin group and olmesartan ester group ( P<0.05), and the differences between groups were statistically significant ( P<0.05) . Conclusion:Dagliprazin combined with olmesartan ester can reduce kidney injury and delay the progression of diabetic nephropathy by regulating the expression of Plin and miR-26a protein and gene in renal tissue of diabetic nephropathy rats.

Objective:To investigate the expression of ras-related C3 botulinum toxin substrate 3 (RAC3) in glioma tissues and its effect on the migration and invasion of glioma cells.Methods:The expression of RAC3 in 57 glioma patients and their adjacent tissues from the First People’s Hospital of Shangqiu was detected by immunohistochemical assay. According to the experimental requirements, brain glioma cells U87MG were divided into experimental group and control group. The experimental group U87MG cells were transfected with RAC3-siRNA plasmid, and the control group U87MG cells were transfected with MOCK-siRNA plasmid. RAC3 mRNA in each group was detected by fluorescence quantitative PCR. The expressions of RAC3 and MMP2 in each group were detected by Western blot. Transwell was used to detect the migration and invasion ability of cells in each group.Results:The positive rate of RAC3 in glioma patients was 89.47% (51/57 cases) , and the expression rate in paracancer tissues was 14.04% (8/57 cases) . The expression rate of RAC3 in glioma tissues was significantly higher than that in paracancer tissues, with statistical significance ( P<0.01) . After siRNA transfection, mRNA expression of RAC3 in experimental group and control group was 1.23±0.20 and 0.43±0.12, and protein expression of RAC3 was 1.19±0.11 and 0.23±0.08, respectively. The expression of MMP2 protein was 1.19±0.11 and 0.23±0.08, respectively. The expression of MMP2 in experimental group was significantly decreased ( P<0.05) . Transwell assay showed that the number of invasive cells in experimental group and control group U87MG cells was (22±5) and (45±8) , and the number of migratory cells was (34±6) and (90±11) , respectively. In experimental group, U87MG cell migration and invasion ability decreased significantly (both P<0.05) . Conclusion:The high expression of RAC3 in glioma tissues may be related to the malignant degree of development, and affect the migration and invasion ability of glioma cells by regulating the expression of MMP2.

Pulmonary blast injury has become the main type of trauma in modern warfare, characterized by externally mild injuries but internally severe injuries, rapid disease progression, and a high rate of early death. The injury is complicated in clinical practice, often with multiple and compound injuries. Currently, there is a lack of effective protective materials, accurate injury detection instrument and portable monitoring and transportation equipment, standardized clinical treatment guidelines in various medical centers, and evidence-based guidelines at home and abroad, resulting in a high mortality in clinlcal practice. Therefore, the Trauma Branch of Chinese Medical Association and the Editorial Committee of Chinese Journal of Trauma organized military and civilian experts in related fields such as thoracic surgery and traumatic surgery to jointly develop the Clinical treatment guideline for pulmonary blast injury ( version 2023) by combining evidence for effectiveness and clinical first-line treatment experience. This guideline provided 16 recommended opinions surrounding definition, characteristics, pre-hospital diagnosis and treatment, and in-hospital treatment of pulmonary blast injury, hoping to provide a basis for the clinical treatment in hospitals at different levels.

Objective:To investigate the changes in the level of microRNA-193b-3p（miR-193b-3p）in patients with aneurysmal subarachnoid hemorrhage（aSAH）after hyperbaric oxygen（HBO）treatment and to explore its significance.Methods:A total of 176 patients with aSAH admitted to the Emergency Department of the First People’s Hospital of Shangqiu from January 2019 to September 2020 were selected as the research subjects. They were divided into sound prognosis group（ n=128）and poor prognosis group（ n=48）according to scores of the modified Rankin Scale（mRS）90 days after treatment. The relative expression levels of miR-193b-3p were detected by real-time quantitative PCR（RT-qPCR）. The prognostic risk factors of aSAH patients were analyzed by logistic regression. The value of miR-193b-3p in judging the prognosis of aSAH patients was evaluated by receiver operating characteristic（ROC）curve. The dose-response relationship between miR-193b-3p and prognosis in aSAH patients was analyzed by the logistic regression using restricted cubic splines. Results:The relative expression levels of miR-193b-3p in the sound prognosis group before and after treatment were higher than those in the poor prognosis group at the same time points（ P<0.05），and the relative expression levels of miR-193b-3p in both groups were decreased after treatment（ P<0.05）. The area under the ROC curve（AUC），sensitivity，and specificity of post-treatment miR-193b-3p in judging the prognosis of aSAH patients were 0.818，68.75%，and 85.94%，respectively. Hunt-Hess Ⅲ-Ⅳ，cerebral vasospasm（CVS），Glasgow Coma scale（GCS）rating，and high C-reactive protein（CRP）level were independent risk factors for aSAH prognosis（ P<0.05）；the relatively high expression level of post-treatment miR-193b-3p was an independent protective factor for aSAH prognosis（ P<0.05）. Post-treatment miR-193b-3p had a non-linear relationship with the prognosis of aSAH（ P<0.05）. Conclusion:After HBO treatment，the relative expression level of miR-193b-3p in aSAH patients was decreased，which is related to the poor prognosis of aSAH.

Objective:To investigate the changes in the level of microRNA-193b-3p（miR-193b-3p）in patients with aneurysmal subarachnoid hemorrhage（aSAH）after hyperbaric oxygen（HBO）treatment and to explore its significance.Methods:A total of 176 patients with aSAH admitted to the Emergency Department of the First People’s Hospital of Shangqiu from January 2019 to September 2020 were selected as the research subjects. They were divided into sound prognosis group（ n=128）and poor prognosis group（ n=48）according to scores of the modified Rankin Scale（mRS）90 days after treatment. The relative expression levels of miR-193b-3p were detected by real-time quantitative PCR（RT-qPCR）. The prognostic risk factors of aSAH patients were analyzed by logistic regression. The value of miR-193b-3p in judging the prognosis of aSAH patients was evaluated by receiver operating characteristic（ROC）curve. The dose-response relationship between miR-193b-3p and prognosis in aSAH patients was analyzed by the logistic regression using restricted cubic splines. Results:The relative expression levels of miR-193b-3p in the sound prognosis group before and after treatment were higher than those in the poor prognosis group at the same time points（ P<0.05），and the relative expression levels of miR-193b-3p in both groups were decreased after treatment（ P<0.05）. The area under the ROC curve（AUC），sensitivity，and specificity of post-treatment miR-193b-3p in judging the prognosis of aSAH patients were 0.818，68.75%，and 85.94%，respectively. Hunt-Hess Ⅲ-Ⅳ，cerebral vasospasm（CVS），Glasgow Coma scale（GCS）rating，and high C-reactive protein（CRP）level were independent risk factors for aSAH prognosis（ P<0.05）；the relatively high expression level of post-treatment miR-193b-3p was an independent protective factor for aSAH prognosis（ P<0.05）. Post-treatment miR-193b-3p had a non-linear relationship with the prognosis of aSAH（ P<0.05）. Conclusion:After HBO treatment，the relative expression level of miR-193b-3p in aSAH patients was decreased，which is related to the poor prognosis of aSAH.

Objective:To investigate the curative effects of early continuous blood purification therapy on moderate to severe acute pancreatitis.Methods:The clinical data of 94 patients with moderate to severe acute pancreatitis who received treatment in the First People's Hospital of Shangqiu, China between January 2018 and October 2019 were retrospectively analyzed. These patients were divided into a control group and an observation group ( n =47/group) according to different treatment methods. The control group was treated with conventional treatment, while the observation group was treated with continuous blood purification therapy based on conventional treatment. The time to abdominal pain and distension relief, the changes of vital signs such as respiration and heart rate, renal function, the levels of liver enzymes, C-reactive protein and interleukin-6, median remission time of systemic inflammatory response syndrome, the Acute Physiology, Age, Chronic Health Evaluation II score, modified Marshall score, and modified CT severity index (MCTSI) were compared between the two groups. Results:The time to abdominal pain relief [(2.28 ± 0.44) d] and the median remission time of systemic inflammatory syndrome [(5.27 ± 0.95) d] were significantly shorter than those in the control group [(6.23 ± 1.01) d and (11.30 ± 1.12) d, t = 34.213 and 28.308, both P < 0.05]. The levels of alanine aminotransferase, blood urea nitrogen, serum creatinine, C-reactive protein and interleukin-6 in the observation group were (28.22 ± 34.38) U/L, (5.73 ± 1.83) mmol/L, (70.26 ± 4.34) μmol/L, (102.66 ± 3.29) mg/L, (110.45 ± 5.50) pg/L respectively, which were significantly lower than those in the control group [(47.26 ± 56.61) U/L, (10.55 ± 3.09) mmol/L, (114.21 ± 6.87) μmol/L, (210.51 ± 10.11) mg/L, (281.77 ± 14.99) pg/L, t = 3.520, 23.724, 81.266, 96.618, 114.005, all P < 0.05]. The Acute Physiology, Age, Chronic Health Evaluation II score, modified Marshall score, and modified CT severity index in the observation group were (11.43 ± 0.66) points, (3.06 ± 0.29) points, (8.16 ± 0.27) points, which were significantly lower than (18.49 ± 1.77) points, (4.92 ± 0.37) points, (9.57 ± 0.35) points respectively in the control group ( t = 39.590, 76.458, 67.484, all P < 0.05). Conclusion:Continuous blood purification therapy can effectively remove metabolites and poisons from the body, shorten the duration of systemic inflammatory response syndrome, promote the recovery of body function, and thereby improve the prognosis of moderate to severe acute pancreatitis.

Objective:To evaluate the prognostic value of combined detection of serum C-reactive protein(CRP), procalcitonin and lactic acid in elderly patients with community acquired pneumonia(CAP).Methods:Ninety-five elderly CAP patients in the emergency department of Shangqiu First People's Hospital were included as the case group, and 45 elderly healthy people in the emergency department of Shangqiu First People's Hospital were enrolled as the control group.Levels of blood lactic acid, procalcitonin and CRP were compared between the two groups.Meanwhile, blood levels of lactic acid, procalcitonin and CRP were compared between patients with different outcomes in the case group.The receiver operating characteristic(ROC)curve was used to evaluate the prognostic value of CRP, procalcitonin and blood lactic acid in elderly patients with CAP.Results:Compared with the control group, blood levels of lactic acid, procalcitonin and CRP were increased in the case group( t=20.77, 26.03 and 31.27, all P<0.01). During a 12-month follow-up, 13 cases(13.68%, 13/95)died and 82 cases(86.32%, 82/95)survived in the case group.Blood levels of lactic acid, procalcitonin and CRP were higher in the death group than in the survival group( t=25.56, 8.30 and 13.56, all P<0.01). ROC curve analysis showed that the sensitivity and specificity of serum lactic acid, procalcitonin and CRP in predicting the prognosis of elderly CAP patients were 76.92% and 73.17%, 84.62% and 78.05%, and 69.23% and 70.73%, respectively.The sensitivity and specificity of combined detection of the three indicators were 92.31% and 89.02%, respectively, higher than those of the individual indicators. Conclusions:Serum levels of lactic acid, procalcitonin and CRP are increased in elderly patients with CAP.Combined detection of the three indicators can improve the prognostic value and therefore has important clinical significance.

Objective To summarize the international current status of anesthesia nursing research. Methods The nursing papers were searched across the databank of PubMed and Web of science between 1946 and 2017 and 2012 and 2017,respectively to look into the external characteristics of international anesthesia nursing publications. Using the methods of common words analysis, cluster analysis and citation analysis, we analyzed high frequency subjects and highly cited articles from 2012-2017, analyzing the highlights in the anesthesia nursing. Results 5,041 articles from 1946-2017 were included. The number of articles in anesthesia nursing field was relatively small in first 30 years,increasing year by year since 1970s and speeding up since 1990s.The average number of articles from 2012-2017 was all above 150.The 5,041 articles distributed in 1,260 journals.The top 10 journals accounted for 24.36% of the total number (1228/5041).The articles were mainly published in the developed countries in Europe and America.The hot spots in anesthesia nursing research included anesthesia management and pain management,professional skills of anesthesia nurses and medical team collaboration,intervention on psychological status and complications among perioperative patients. Conclusions The research of anesthesia nursing aboard has attracted more and more attention and formed several research highlights.Domestic research in the field is still at the early stage and in the future,domestic scholars can draw on foreign research highlights to carry out relevant research.